LOCUS       sequence-461125-        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of opsin 'TsChR', regulated by rab-3 
            promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::TsChR
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6315..6337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6437..6456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6534..6552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6620..6724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6725..6793,6794..7585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6943..6962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7756..8344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8245..8264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8498..8515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8632..8653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8668..8673,8674..8691,8692..8698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8706..8722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8711..8733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8730..8746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8730..8746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgttcgcca tcaaccccga atatatgaat
     4561 gagactgtgc tgctggacga atgcacccca atctacctgg atattggacc actgtgggag
     4621 caggtggtgg ctagggtgac tcagtggttc ggagtgatcc tgtctctggt gtttctgatc
     4681 tactacatct ggaacactta caaggctacc tgtgggtggg aggaactgta cgtgtgcaca
     4741 gtggagttct gtaagatcat catcgaactg tacttcgagt atactccccc tgccatgatc
     4801 ttccagacaa atggacaagt gactccctgg ctgcggtatg ctgagtggct gctgacatgc
     4861 cctgtgatcc tgattcacct gtctaacatt actgggctga atgacgatta cagtggccgc
     4921 accatgagcc tgatcacatc cgacctgggc ggaatttgta tggctgtgac cgccgctctg
     4981 agcaagggat ggctgaaagc cctgttcttt gtgatcgggt gcggctacgg agccagcacc
     5041 ttctacaacg ccgcttgtat ctacattgag tcctactata ccatgccaca gggaatttgc
     5101 aggagactgg tgctgtggat ggccggggtg ttctttacat cctggttcat gtttcctggc
     5161 ctgttcctgg ctggaccaga aggaacccag gccctgtctt gggctggaac cacaatcgga
     5221 catacagtgg ccgacctgct gagtaagaac gcttggggaa tgatcgggca ctttctgagg
     5281 gtggagatcc acaagcatat catcatccat ggagatgtgc ggcgccccgt gacagtgaag
     5341 gctctgggga ggcaggtgag cgtgaattgt ttcgtggaca aagaggaaga ggaagaggat
     5401 gagagaatct aagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     5461 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     5521 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     5581 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     5641 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     5701 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     5761 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     5821 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     5881 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     5941 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     6001 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     6061 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     6121 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     6181 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     6241 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     6301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6421 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6481 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     6541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7261 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7321 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7381 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7441 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7501 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7561 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7621 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7681 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7861 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8161 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8461 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8521 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8581 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8641 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8701 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     8761 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
     8821 aaaatggctg aaatcactca caacgatgga tac
//
