LOCUS       sequence-461130-        4535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 'PRDX-2' on activation via 'QF+hGR' chimeric protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-5xQUAS::Delta-pes-10P::AI::prdx-2G
FEATURES             Location/Qualifiers
     source          1..4535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1957..1979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2079..2098
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2176..2194)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2262..2366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2367..2435,2436..3227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2585..2604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3398..3986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3887..3906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4140..4157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4274..4295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4310..4315,4316..4333,4334..4340)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4348..4364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4353..4375
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4372..4388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4372..4388
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gggtaatcgc ttatcctcgg ataaacaatt atcctcacgg gtaatcgctt atccgctcgg
       61 gtaatcgctt atcctcgggt aatcgcttat ccttaagctt gatatccctg caggatcgat
      121 tttttgcaaa ttacgagcgt tgtagggggc ggagcgatag gtcctatagg ttttggtata
      181 tcatcattca ttcattcatt ggtacattca tttacccacc ttcctctttc tgagcttctc
      241 tggagttctg tgcttccttt ttcccttatc tttatactgt aatttttaac tttcagggat
      301 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
      361 cattttcagg aggacccttg gagggtaccg agctcagaaa aaatgtcgaa agcattcatc
      421 ggaaagccag ctccacaatt caagactcaa gccgtcgttg atggcgagtt cgttgatgtt
      481 tcgctctctg actacaaggg aaaatacgtt gtgctcttct tctacccact tgacttcact
      541 ttcgtgtgcc caaccgagat tatcgccttc tctgaccgtg ctgaggagtt caaggctatc
      601 aacaccgttg tgctcgccgc ttccaccgac tctgtcttct ctcacttggc atggatcaac
      661 cagccacgca agcacggagg actcggagag atgaacattc cagttctcgc tgacaccaac
      721 caccaaatct cccgtgatta cggagttctc aaggaggacg aaggaattgc tttccgtggt
      781 gagttatatt ctgaatggaa tccttcatat tttcgtactt tatttgaaaa ataaaatttc
      841 aggactcttc atcatcgacc catcacaaaa cctccgtcaa atcaccatca atgatcttcc
      901 agtcggacgc tctgttgatg agactcttcg tcttgttcag gccttccagt tcgtcgagaa
      961 gcacggagag gtttgcccag ctggatggac tccaggatcc gacaccatca agccaggagt
     1021 caaagaaagc caagagtact tcaagaagca ctaagcgccg gtcgctacca ttaccaactt
     1081 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac
     1141 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt
     1201 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
     1261 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct
     1321 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa
     1381 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt
     1441 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca
     1501 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     1561 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     1621 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     1681 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt
     1741 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag
     1801 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg
     1861 acagaataca ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc
     1921 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1981 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2041 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     2101 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     2161 caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2221 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2341 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2401 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2461 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2521 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2581 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2641 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2701 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2761 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2821 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2881 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2941 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3001 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3061 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3121 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3181 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4201 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4261 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4321 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4381 gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc
     4441 tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa
     4501 caacttggaa atgaaataag cttgcatgcc tgcag
//
