LOCUS       sequence-461138-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of voltage sensor 'Voltron', regulated by 
            rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::Voltron
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7092..7114)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7214..7233
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7311..7329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7397..7501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7502..7570,7571..8362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7720..7739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8533..9121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9022..9041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9275..9292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9409..9430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9445..9450,9451..9468,9469..9475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9483..9499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9488..9510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9507..9523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9507..9523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atggctgacg tggaaaccga gaccggcatg
     4561 attgcacagt ggattgtctt tgctattatg gctgctgctg ctattgcttt tggagtggct
     4621 gtgcactttc ggccttcaga gctgaagagc gcatactata tcaacattgc catctgcact
     4681 atcgccgcta ccgcttacta tgcaatggcc gtgaactacc aggacctgac aatgaatggt
     4741 gaaaggcagg tggtctacgc aagatatatt aactgggtgc tgaccacacc actgctcctg
     4801 ctcgatctca tcgtcatgac caagatgggc ggagtgatga tttcttgggt catcggcgca
     4861 gacattttca tgatcgtgtt tggtattctg ggcgccttcg aggatgaaca caagttcaaa
     4921 tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc tgacatacgg gatgtataac
     4981 gccacttgga aagacgatct gaagaaaagc cccgagtacc atagctccta tgtcagtctg
     5041 ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg tgtgggcttt cgggtctggt
     5101 agtggcgtgc tgtccgtcga caatgaggcc attctcatgg gaatcctgga tgtgctcgct
     5161 aagccactgt ttggaatggg gtgcctcatt gcccatgaga ctatcttcaa gatcggtact
     5221 ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat
     5281 gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac
     5341 gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc tccagacctg
     5401 atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga ccacgtccgc
     5461 ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt cattcacgac
     5521 tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt
     5581 gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga atttgcccgc
     5641 gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat cgatcagaac
     5701 gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga agtcgagatg
     5761 gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg gcgcttccca
     5821 aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga agaatacatg
     5881 gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc aggcgttctg
     5941 atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac
     6001 atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg cagcgagatc
     6061 gcgcgctggc tgtcgacgct cgagatttcc ggcgagccaa ccactaagag caggatcacc
     6121 agcgagggcg agtacatccc cctggaccag atcgacatca acgtgttctg ctacgagaac
     6181 gaggtgtaag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc
     6241 cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt
     6301 tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     6361 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
     6421 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     6481 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     6541 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
     6601 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     6661 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     6721 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     6781 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
     6841 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
     6901 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
     6961 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
     7021 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat
     7081 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7141 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7201 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     7261 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat
     7321 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7381 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7441 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7501 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7561 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7621 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7681 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7741 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7801 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7861 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7921 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7981 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8041 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8101 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8161 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8221 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8281 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8341 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8641 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9181 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9241 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9301 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9361 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9421 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9481 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     9541 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa
     9601 atggctgaaa tcactcacaa cgatggatac
//
