LOCUS       sequence-461139-       11289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing 
            construct into the C. neoformans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11289)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2647
FEATURES             Location/Qualifiers
     source          1..11289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1058..1074
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1059..1075
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2887..2940)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6167..6730)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     primer_bind     complement(7564..7583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7565..7583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7590..7607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7590..7606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7599..7621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7747..8202
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7834..7853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8044..8065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8333..9136)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9137..9352)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9376..9395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9495..9517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9555..9573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9614..10117
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10154..10258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10259..10327,10328..11119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10477..10496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctcg aggtcgacgg tatcgatcgt
     1081 tcaatctcat agtcttctaa agatcccccg ttcccaaaca tgacattcaa catcttcgct
     1141 tcgacttcca cctcgaccaa ccccttttct tccaaatcca ctcgacgtgc gccatccccc
     1201 tcttcctgcc ccatccctcc tccgtttgcc ccgccaactc ttcccaggtt gatgaagggg
     1261 gtgccctggc ccgacaagcg tcgaggcgag gtaagtgtgt ggctgagatt caaggcatta
     1321 acgaggacgt gtgagcaggt ggaagtgcgg ttgcggtagg aaggacgagg tcagggatgc
     1381 gtagagacat cggtaattct gtacatctgt ttcttgcttc tttccccgtt ctttccatgc
     1441 aacaaactat tattacaaca tgaactctct tagcatgaca gtccacagaa ccgagatggt
     1501 cagtgcatgt cgagattatt tcgtggtgat ctccaggaac gactgaaaaa aggagtgacg
     1561 gattaaggaa gacgtgaccg actaaaggaa aggaaggatg gaaggaacga tatcaatgcg
     1621 atggtggcga cctgagaatg agtaaatagt tgaaagcact aaaataaagc agcaacgaag
     1681 gttattccaa gttatagctc tcgtgcaaat ttcaacagtg atggccattt tattgttgca
     1741 agagacgtcg tcagccgaga aatattcgat ttcgcggaca actatcggga atccaataac
     1801 ggacaaacga catggaatga tatatcacgg caatgaagat tttgaagcgc agaggtatac
     1861 atcagcaaat aagacacaaa acgtaagaag cacaagtagc aacagtcatc cgatgcatat
     1921 tacgcgtaag aagggtcact acataagtaa ttaggaggag gtcttctgct gggtcatcat
     1981 gttctttcgg tagtttcgga cggggggcca accgacgacc tgggccttgg cggggggctt
     2041 ggaggggtcc ttgagggtgg tcttctcctt ggggacggcg gagacgttct tgaggtcgac
     2101 ggaaccctcc ttgttggact ggaggttgag catgaggtcg acggtctcgg agaaaccact
     2161 acctccggat ccagaaccct tgtagagctc gtccataccc atgacgtcgg tgaaggcctt
     2221 ctgccactcc ttgaagttga gctcggtctt ggagtgcttg agctcggtct ttcggaagac
     2281 gtacatgggc tggttcttga ggtagttggc ggccatgggc ttggcgaagg tgtaggtggt
     2341 tcgggcggtg gatcggtatc gcttaccgtt accggtggtg taggaccact tgaaggtgga
     2401 gatgatggtc ttgtcgttgg ggtaggtctt cttggatcga caccagtcgg cggcggtgag
     2461 ggagttggtc atgacgggac cgtcggcggg gaaaccggta cccttgacct gggcctcacc
     2521 cttgatgtgg gaaccctcgt aggtgtatcg gtagttgacg gtgagggagg caccgtcctc
     2581 gaactgcatg gttcggtgga cctggtaacc ggaaccgtcg accatggcgg cctggaaggg
     2641 ggacataccg tcggggtagg ggaggtactg gtggaaaccg taaccgatgt gggggacgag
     2701 gatccagggg gagaactgga ggtcaccctt ggtggacttg aggttgagct cctcgtaacc
     2761 gtcgttgggg ttaccggtac cctgaccgac catgtcgaag tcgacaccgt tgatggaacc
     2821 gaagatgtgg agctcgtggg tggcggggag ggaggccatg ttgtcctcct cacccttgga
     2881 gaccatggga ccggggttct cctcgacgtc accacaggtg aggagggaac ctcgaccctc
     2941 tccgcttcct gatctgagga tcttgacgaa gttgggggcg tcgtctcggg caccggcggt
     3001 ggttcggtag acgtagagct tctcgacctt ggggaggtca ccgtggttct cctccatctc
     3061 gttggaaccg tctcgctcgt tgatgacctc gacgttgagc atgggcatct tggaggcgag
     3121 gacctgacaa cccttgaggg tgacgttaca ggaggacatc cagagggatc gcatggtctc
     3181 gtatcgggcg aagttaccga ggagggcggc gtcaccgaag ggggagtctc ggatctcgag
     3241 ctttcggagg ttcttacaac cgttcatgac gtgcatcata cccttgtcgg agtcaccggc
     3301 gaaggcgatg gagagcatct cgagctgctt ggcgtactta ccgatgtaca tgaagacctt
     3361 gtcggtgagg agaccggaga tggagagtcg ctggagaccc ttacactctc ggacgatggc
     3421 accgaaaccc tcgtcgaggg gctgggaggt gacgacgtcg ggcttaccgg gctcgaggat
     3481 acagagtcgg aatcgggtga agttgggaca gttcttggcg acggtgacga gggcggcgtt
     3541 ggtcatctgg tgacagaagt agaggaggga gttgagcttg ggacaaccga gggagacggc
     3601 gacgagaccc tcctcggtga cggcggagta accggcgacg tagaagtcgg aggggaagac
     3661 tcggagctcc tggaggtcct tacaggagga ggcgacgacc tggagaccct tgtcggagat
     3721 acagtcgagg acccagagtc gctggagctt gacacatcgg gagatcatct tggtgaggtc
     3781 ggaggcgtcg agggtggggg cgtaggagag gttgagaccg gtgagctggg cacagagggg
     3841 gtagatgaag gagagacaga cgggggaggc gtcccagaaa ccggagaggg atcggagcat
     3901 cttacacttc tcgagggcgg aggtgagctt gaagtaggac tcggtctgga agtcgtcggt
     3961 gaggttaccg gtaccgaggt cctcgaggtt gggggttcgg aggaggatct tggcgagggt
     4021 gtcgacggag acggatcggt tgagtcggag ggatcggagg ttgggggatc gggagacgag
     4081 tcgctcgagg gaaccggcgt tgacctcacc cttgatacag gcgaagttga gggagacgag
     4141 ggaggtacag gagtcgggga aacaggagag ccatcgggga cctcggtcct cgacctcgtt
     4201 ctcctggagg tcgagctctc ggaggagctt acagtgctgt cgggagatgt tagtggagta
     4261 acatgttgcg ggaaacgggg ttacatacgg aggcgacggc ggcgagaccg tcggtggaga
     4321 aaccctcaca ggagatgagg acgagggctc ggaatcgggg gaaggatcgg gcgaggagct
     4381 cgagggactc gtcggagacg accattcgct tcattcggag ctcctcgaga ccgtgacaac
     4441 ctcgggcggc ggcctcgatc cagggaccgg cgtaaccacc ccagtcgggg gggacgaggt
     4501 tgaagtcggc accgtggggc ttacccttga cggtgagggc tcggacgttg gggaatcggg
     4561 cggcgactcg accggctcgg acggcgtaac agttaccgac gaagacacct cgtcgggaga
     4621 gtcgctcgat ctcgtaccag accttacaga cgagggagac ggtgtttcgg tctcgctggg
     4681 cggggaggaa ggagaagatg tgctcgacga cctcctcggg gaagtaggtc attttgaagt
     4741 tttctgtgga gatcgttagc atgtgcgaag aggcaatgat aggacaataa agtgttaggg
     4801 cgttgtgagt tggaagggca tgtatccgat gggttgaaaa ttttcaagag ccaccaaatt
     4861 tttttcttca ccacacacaa gcccatgttg agctcagtag tggcaaaaca gtactctgca
     4921 gtcgacgcaa cagatgaggg caaaagcatg gaagacatcg ccaaaaacaa gacagaatga
     4981 tgtgggtggt cgaccatgct cagctccact ctgccccgac ttcacctgca gaagccacca
     5041 gcgagaattg ttttacgtgc cctacgactg gcgcggattt tcaaaggtgt caaaagactt
     5101 tgggtactca cctaggtgtt ttggttaaag aatgtagaga taaagaaggt ggtgggagaa
     5161 aggaatgtgc gagggattta attctttccg gttgatgggc agggcggatt ctcggattaa
     5221 ttcagaaaat cccacaattt ttttcgaaac ctccagaatg tgaaaagctc actggaaatt
     5281 tcatccgtat caaactccgt ttttcaccga gtgtggcaca tgggacttag gaggcaagaa
     5341 atgtgaggag gatggagcga ccgcaacaac ggagaaattt aaagtggagg tcgttcccag
     5401 gcacgtcttc gcaccaaaaa ttcaacgggt ggcaagaggc ccctgccgtc taaacatgtg
     5461 ggagtggctc atcctcacac cgagcaggcc aatgcatatg ataagagtcg tccagaagcc
     5521 gacgtgcatg aatggagctt cacaaaatgc gaaattagaa gaagacgaga tgcccatgat
     5581 cttctgggaa gggggcgagg caacttgctt ggcgaatggg gctgaatatt ggggcgttcg
     5641 ctaatggcca tgacgcttgt tcatggcagg gtagaacacg acattattgc ttgtgcgtgg
     5701 gggcttctgg ctgctttttg tacattgcgc gaagggttgc catgatgaga ctgctgtatt
     5761 atgctcttat tcttttgttt aaagggagtg atacttgaca taccgatgat agatagttaa
     5821 gaagcgcccg caggtgggtc aaccacactt gcgaccattg ttaatcattc agaacggtcg
     5881 acaaggtcag tgtaagatca ttcgctatgg agcttgcata gtagccacaa tcttgtaccg
     5941 tatagcatag aatggccatg aagatcctga ggattcaacg gcgttgctga gggtgggaag
     6001 gatggtattg ttgtctgtag atagattaat gggcatataa tgtcaaaaaa gatgattgac
     6061 acgatgtctg tctcgaattc gcagtgtgct aaaattgtat cacttctcta atatgtatat
     6121 atacaccctc taaggaaaat taacccctta ccgccttcac gaattcttag gggcagggca
     6181 tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc gtcgtacagg gcggtgtcca
     6241 ggccgcagag ggtgaacccc atccgccggt acgcgtggat agccggtgcg ttaacgttgg
     6301 tgacctccag ccagaggtgc ccggcgcccc gctcgcgggc gaactccgtc gcgagcccca
     6361 tcaacgcgcg cccgaccccg tgcccccggt gctccggggc gacctcgatg tcctcgacgg
     6421 tcagccggcg gttccagccg gagtacgaga cgaccacgaa gcccgccagg tcgccgtcgt
     6481 ccccgtacgc gacgaacgtc cgggagtccg ggtcgccgtc ctccccgtcg tccgattcgt
     6541 cgtccgattc gtcgtcgggg aacaccttgg tcaggggcgg gtccaccggc acctcccgca
     6601 gggtgaagcc gtccccggtg gcggtgacgc ggaagacggt gtcggtggtg aaggacccat
     6661 ccagtgcctc gatggcctcg gcgtcccccg ggacactggt gcggtaccgg taagccgtgt
     6721 cgtcaagagt ggcggccgcc atagacatgt tgggcgagtt tactaatgga aaaagacaat
     6781 tagcaaagag gctgcatatt aggagacgcg gtgaagattg gggaggacaa tagggtccgt
     6841 gcaccaaaaa gtcctgacgg cagctctgtt caacgcacat ccatctcttc cagctatgcg
     6901 tgtggcacca gcacggatgg attgttgcac caaaaggaca aggcgagatg gatgcaggtc
     6961 gaggcgatgc ggtgctatcg gccctcgttg tcgaacgcca cgtcgtcctc tcggcgaccg
     7021 tcaatcctgg aaggaaatga ccaagcgtgc acccagcatc cagcatccaa catccagcac
     7081 ccagcaccta gcacccatga tccaatggcc ggcatgcaga tcagcgtggg gagagcacca
     7141 tctagcagcc cctggcaggc ccagcattgt gctcggatgt gcgataggcg gtgggtgcga
     7201 taaggtggat tagtggagga tggaggactg agtggcgggc aagggaaggg ggcgggaaga
     7261 tgactcacct tgggcagtgg gtgggggatg cggtataggg gatgtatgtg gatgctggcg
     7321 gaggatagaa gctgtaagct gagctggtgc caacggtgac gctgtgagag tggtctcggc
     7381 gaccctgacg gcgactcggc gttaccccgt gtggcacacc ggacgcgcgt cgagtgacgt
     7441 tgctgctatt gttgttacca tcatcctctc ctccgacaac catacactca gtgcacacca
     7501 tgccactcga atcctgcatg cacagttcta gagcggccgc caccgcggtg gagctccaat
     7561 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     7621 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7681 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7741 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7801 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7861 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7921 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7981 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8041 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8101 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8161 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     8221 ccttacgcat ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg
     8281 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     8341 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     8401 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     8461 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     8521 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     8581 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     8641 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     8701 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     8761 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     8821 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     8881 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     8941 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     9001 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     9061 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     9121 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     9181 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     9241 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     9301 ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaaggtggt
     9361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     9421 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     9481 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     9541 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9601 ggtttcttag acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg
     9661 aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga
     9721 aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta
     9781 aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta
     9841 aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg
     9901 gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa
     9961 gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa
    10021 aactattttt tctttaattt ctttttttac tttctatttt taatttatat atttatatta
    10081 aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt
    10141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10261 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10321 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10381 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10441 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10501 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10561 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10621 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10681 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10741 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10801 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10861 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10921 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10981 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11041 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11101 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11281 aggatcttc
//
