LOCUS       sequence-461140-        9726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Flp-IN human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pFRT-FLAG-HA-(N)-CDK12
FEATURES             Location/Qualifiers
     source          1..9726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..288
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     239..259
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     333..352
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        333..351
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      416..425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             425..448
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             449..475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    491..515
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4992..5016)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5069..5110
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5148..5165)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5154..5378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5511..5530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5662..5709
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5717..6739
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5717..6739
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6869..6990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6906..6925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6960..6979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7039..7055)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7039..7055)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7052..7074)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7063..7079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7087..7093,7094..7111,7112..7117))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7132..7153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7270..7287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7441..8029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7521..7540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8200..8991,8992..9060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8823..8842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9061..9165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9240..9259)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9431..9726,1..84)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
       61 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      121 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      181 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      241 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      301 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      361 gctggctagc gagctcggat cgatccatcg atagtgtggt ggaattctga tcgccgccgc
      421 catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt acgctttgaa
      481 ccagatatca acaagtttgt acaaaaaagc aggcttcatg cccaattcag agagacatgg
      541 gggcaagaag gacgggagtg gaggagcttc tggaactttg cagccgtcat cgggaggcgg
      601 cagctctaac agcagagagc gtcaccgctt ggtatcgaag cacaagcggc ataagtccaa
      661 acactccaaa gacatggggt tggtgacccc cgaagcagca tccctgggca cagttatcaa
      721 acctttggtg gagtatgatg atatcagctc tgattccgac accttctccg atgacatggc
      781 cttcaaacta gaccgaaggg agaacgacga acgtcgtgga tcagatcgga gcgaccgcct
      841 gcacaaacat cgtcaccacc agcacaggcg ttcccgggac ttactaaaag ctaaacagac
      901 cgaaaaagaa aaaagccaag aagtctccag caagtcggga tcgatgaagg accggatatc
      961 gggaagttca aagcgttcga atgaggagac tgatgactat gggaaggcgc aggtagccaa
     1021 aagcagcagc aaggaatcca ggtcatccaa gctccacaag gagaagacca ggaaagaacg
     1081 ggagctgaag tctgggcaca aagaccggag taaaagtcat cgaaaaaggg aaacacccaa
     1141 aagttacaaa acagtggaca gcccaaaacg gagatccagg agcccccaca ggaagtggtc
     1201 tgacagctcc aaacaagatg atagcccctc gggagcttct tatggccaag attatgacct
     1261 tagtccctca cgatctcata cctcgagcaa ttatgactcc tacaagaaaa gtcctggaag
     1321 tacctcgaga aggcagtcgg tcagtccccc ttacaaggag ccttcggcct accagtccag
     1381 cacccggtca ccgagcccct acagtaggcg acagagatct gtcagtccct atagcaggag
     1441 acggtcgtcc agctacgaaa gaagtggctc ttacagcggg cgatcgccca gtccctatgg
     1501 tcgaaggcgg tccagcagcc ctttcctgag caagcggtct ctgagtcgga gtccactccc
     1561 cagtaggaaa tccatgaagt ccagaagtag aagtcctgca tattcaagac attcatcttc
     1621 tcatagtaaa aagaagagat ccagttcacg cagtcgtcat tccagtatct cacctgtcag
     1681 gcttccactt aattccagtc tgggagctga actcagtagg aaaaagaagg aaagagcagc
     1741 tgctgctgct gcagcaaaga tggatggaaa ggagtccaag ggttcacctg tatttttgcc
     1801 tagaaaagag aacagttcag tagaggctaa ggattcaggt ttggagtcta aaaagttacc
     1861 cagaagtgta aaattggaaa aatctgcccc agatactgaa ctggtgaatg taacacatct
     1921 aaacacagag gtaaaaaatt cttcagatac agggaaagta aagttggatg agaactccga
     1981 gaagcatctt gttaaagatt tgaaagcaca gggaacaaga gactctaaac ccatagcact
     2041 gaaagaggag attgttactc caaaggagac agaaacatca gaaaaggaga cccctccacc
     2101 tcttcccaca attgcttctc ccccaccccc tctaccaact actacccctc cacctcagac
     2161 accccctttg ccacctttgc ctccaatacc agctcttcca cagcaaccac ctctgcctcc
     2221 ttctcagcca gcatttagtc aggttcctgc ttccagtact tcaactttgc ccccttctac
     2281 tcactcaaag acatctgctg tgtcctctca ggcaaattct cagccccctg tacaggtttc
     2341 tgtgaagact caagtatctg taacagctgc tattccacac ctgaaaactt caacgttgcc
     2401 tcctttgccc ctcccaccct tattacctgg agatgatgac atggatagtc caaaagaaac
     2461 tcttccttca aaacctgtga agaaagagaa ggaacagagg acacgtcact tactcacaga
     2521 ccttcctctc cctccagagc tccctggtgg agatctgtct cccccagact ctccagaacc
     2581 aaaggcaatc acaccacctc agcaaccata taaaaagaga ccaaaaattt gttgtcctcg
     2641 ttatggagaa agaagacaaa cagaaagcga ctgggggaaa cgctgtgtgg acaagtttga
     2701 cattattggg attattggag aaggaaccta tggccaagta tataaagcca aggacaaaga
     2761 cacaggagaa ctagtggctc tgaagaaggt gagactagac aatgagaaag agggcttccc
     2821 aatcacagcc attcgtgaaa tcaaaatcct tcgtcagtta atccaccgaa gtgttgttaa
     2881 catgaaggaa attgtcacag ataaacaaga tgcactggat ttcaagaagg acaaaggtgc
     2941 cttttacctt gtatttgagt atatggacca tgacttaatg ggactgctag aatctggttt
     3001 ggtgcacttt tctgaggacc atatcaagtc gttcatgaaa cagctaatgg aaggattgga
     3061 atactgtcac aaaaagaatt tcctgcatcg ggatattaag tgttctaaca ttttgctgaa
     3121 taacagtggg caaatcaaac tagcagattt tggacttgct cggctctata actctgaaga
     3181 gagtcgccct tacacaaaca aagtcattac tttgtggtac cgacctccag aactactgct
     3241 aggagaggaa cgttacacac cagccataga tgtttggagc tgtggatgta ttcttgggga
     3301 actattcaca aagaagccta tttttcaagc caatctggaa ctggctcagc tagaactgat
     3361 cagccgactt tgtggtagcc cttgtccagc tgtgtggcct gatgttatca aactgcccta
     3421 cttcaacacc atgaaaccga agaagcaata tcgaaggcgt ctacgagaag aattctcttt
     3481 cattccttct gcagcacttg atttattgga ccacatgctg acactagatc ctagtaagcg
     3541 gtgcacagct gaacagaccc tacagagcga cttccttaaa gatgtcgaac tcagcaaaat
     3601 ggctcctcca gacctccccc actggcagga ttgccatgag ttgtggagta agaaacggcg
     3661 acgtcagcga caaagtggtg ttgtagtcga agagccacct ccatccaaaa cttctcgaaa
     3721 agaaactacc tcagggacaa gtactgagcc tgtgaagaac agcagcccag caccacctca
     3781 gcctgctcct ggcaaggtgg agtctggggc tggggatgca ataggccttg ctgacatcac
     3841 acaacagctg aatcaaagtg aattggcagt gttattaaac ctgctgcaga gccaaaccga
     3901 cctgagcatc cctcaaatgg cacagctgct taacatccac tccaacccag agatgcagca
     3961 gcagctggaa gccctgaacc aatccatcag tgccctgacg gaagctactt cccagcagca
     4021 ggactcagag accatggccc cagaggagtc tttgaaggaa gcaccctctg ccccagtgat
     4081 cctgccttca gcagaacaga cgacccttga agcttcaagc acaccagctg acatgcagaa
     4141 tatattggca gttctcttga gtcagctgat gaaaacccaa gagccagcag gcagtctgga
     4201 ggaaaacaac agtgacaaga acagtgggcc acaggggccc cgaagaactc ccacaatgcc
     4261 acaggaggag gcagcagcat gtcctcctca cattcttcca ccagagaaga ggccccctga
     4321 gccccccgga cctccaccgc cgccacctcc accccctctg gttgaaggcg atctttccag
     4381 cgccccccag gagttgaacc cagccgtgac agccgccttg ctgcaacttt tatcccagcc
     4441 tgaagcagag cctcctggcc acctgccaca tgagcaccag gccttgagac caatggagta
     4501 ctccacccga ccccgtccaa acaggactta tggaaacact gatgggcctg aaacagggtt
     4561 cagtgccatt gacactgatg aacgaaactc tggtccagcc ttgacagaat ccttggtcca
     4621 gaccctggtg aagaacagga ccttctcagg ctctctgagc caccttgggg agtccagcag
     4681 ttaccagggc acagggtcag tgcagtttcc aggggaccag gacctccgtt ttgccagggt
     4741 ccccttagcg ttacacccgg tggtcgggca accattcctg aaggctgagg gaagcagcaa
     4801 ttctgtggta catgcagaga ccaaattgca aaactatggg gagctggggc caggaaccac
     4861 tggggccagc agctcaggag caggccttca ctgggggggc ccaactcagt cttctgctta
     4921 tggaaaactc tatcgggggc ctacaagagt cccaccaaga gggggaagag ggagaggagt
     4981 tccttactaa gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     5041 ctcaagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     5101 cgattctacg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5161 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5221 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5281 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5341 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5401 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5461 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5521 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5581 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5641 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     5701 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     5761 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     5821 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     5881 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     5941 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     6001 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     6061 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     6121 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6181 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6241 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6301 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6361 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6421 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6481 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6541 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6601 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6661 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6721 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     6781 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6841 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6961 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7021 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7201 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9181 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9241 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9301 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9361 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     9421 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9481 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9541 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9601 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9661 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9721 tggccc
//
