LOCUS       sequence-461143-       10278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of 'PRDX-2' using a genomic fragment and 
            fluorophore 'tagRFP', regulated by rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::prdx-2G::SL2::his-24::tagRFP
FEATURES             Location/Qualifiers
     source          1..10278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6085..6795
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7740..7762)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7862..7881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7959..7977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8045..8149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8150..8218,8219..9010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8368..8387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9181..9769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9670..9689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9923..9940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10057..10078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10093..10098,10099..10116,10117..10123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10131..10147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10136..10158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10155..10171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10155..10171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgtcgaaag cattcatcgg aaagccagct
     4561 ccacaattca agactcaagc cgtcgttgat ggcgagttcg ttgatgtttc gctctctgac
     4621 tacaagggaa aatacgttgt gctcttcttc tacccacttg acttcacttt cgtgtgccca
     4681 accgagatta tcgccttctc tgaccgtgct gaggagttca aggctatcaa caccgttgtg
     4741 ctcgccgctt ccaccgactc tgtcttctct cacttggcat ggatcaacca gccacgcaag
     4801 cacggaggac tcggagagat gaacattcca gttctcgctg acaccaacca ccaaatctcc
     4861 cgtgattacg gagttctcaa ggaggacgaa ggaattgctt tccgtggtga gttatattct
     4921 gaatggaatc cttcatattt tcgtacttta tttgaaaaat aaaatttcag gactcttcat
     4981 catcgaccca tcacaaaacc tccgtcaaat caccatcaat gatcttccag tcggacgctc
     5041 tgttgatgag actcttcgtc ttgttcaggc cttccagttc gtcgagaagc acggagaggt
     5101 ttgcccagct ggatggactc caggatccga caccatcaag ccaggagtca aagaaagcca
     5161 agagtacttc aagaagcact aaggtaccgc tgtctcatcc tactttcacc tagttaactg
     5221 cttgtcttaa aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta
     5281 tataaatggt ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt
     5341 tcctcgtttt tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct
     5401 aattttaatt cagggaaact gcttcaacgc atcatgtctg attccgctgt tgttgccgcc
     5461 gctgtcgagc caaaggtccc aaaggctaag gccgccaagg ccgccaagcc agccgccaag
     5521 gccgccaagc caaccaaagt tgccaaggcc aaggctccag tcgctcaccc accatacatc
     5581 aacatgatca aggaggccat caagcagctc aaggaccgca aaggagcttc caagcaggca
     5641 atcctcaagt tcatctccca aaactacaag ctcggagaca atgtcatcca gatcaatgct
     5701 catctccgtc aggctctcaa gcgtggtgtc accagcaagg cccttgttca agccgccgga
     5761 tccggagcca acggacgttt ccgtgtgcca gagaaggccg ccgccgccaa gaagccagca
     5821 gcagccaaga agccagcagc agccaagaaa ccagcagccg ccaagaaagc cactggagag
     5881 aagaaggcaa agaagccagc cgctgccaag ccaaagaagg ccgccaccgg agataagaag
     5941 gtcaagaagg ccaagagccc aaagaaggtt gccaagccag ctgccaagaa ggtcgccaag
     6001 tctccagcca agaaggccgc tccaaagaag atcgccaagc cagctgccaa gaaggccgcc
     6061 aagccagccg ccaaggccgc atgcatggtg tctaagggcg aagagctgat taaggagaac
     6121 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     6181 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     6241 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
     6301 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     6361 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     6421 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     6481 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
     6541 cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc
     6601 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     6661 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     6721 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     6781 gggcacaaac ttaattccgg actcagatct cgagctcaag cttcgaattc caactgagcg
     6841 ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag
     6901 agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc
     6961 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     7021 ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt
     7081 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     7141 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     7201 aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt
     7261 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     7321 gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc
     7381 gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa
     7441 ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg
     7501 tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt
     7561 ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt
     7621 ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt
     7681 cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     7741 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7801 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     7861 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     7921 tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt
     7981 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     8041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8161 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8221 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8281 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8341 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8401 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8461 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8521 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8581 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8641 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8701 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8761 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9001 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9121 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9901 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9961 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    10021 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10081 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10141 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac
    10201 atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
    10261 actcacaacg atggatac
//
