LOCUS       sequence-461145-       11533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of 'GUR-3' using a genomic fragment, 
            regulated by rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::gur-3G
FEATURES             Location/Qualifiers
     source          1..11533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8995..9017)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9117..9136
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9214..9232)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9300..9404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9405..9473,9474..10265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9623..9642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10436..11024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10925..10944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11178..11195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11312..11333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11348..11353,11354..11371,11372..11378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11386..11402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11391..11413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11410..11426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11410..11426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgacgataa cagcgtcgaa tacgttggag
     4561 ttcaaatgga cgtcaccaag gagctcgaga tcgtcattca gtaagttcac tggaatgaac
     4621 ctgtgcagcc ttttattttt caacattgtt tataggaaca acgacagatg cggaacagaa
     4681 aatatcaatt gacatgagta atacttactg tgatcaggta aatgatacag tgatggttcg
     4741 actctaaaaa ttactgagat ttcgaataac taaaaacagt tttttggaat gattaaataa
     4801 gtaaaaattc taaatgtatc aacaatacgt gttgttttga ataattgaac ataaaagaaa
     4861 ataagaggag acaaagtgtg ttaagttgtg aaattttgac tgaagcgaca cattttcatc
     4921 aaagggtaat aatatgaaaa ccataatagc cctacatttt tgtgggtgca ggtatatttg
     4981 agactgcacg cacggtgtgc ctagatttat ttttagtttc aaatattttt catatttccg
     5041 aacgaatagt ggaagcagca aaatgtattt gtagcatcaa gtatttctag ccattttgca
     5101 atttttatat tgttcagttc taatgaagat tgcagaaaaa aacggtaaca aacgcttatt
     5161 caagaacaaa ttgtatatgt tcaggttttg gggcctctgt actcctatat gatggtgttg
     5221 ggacttaatc acactcacag tagcgctcgg aatacaatgt ttaagtggcc attgacaatc
     5281 tacaattatc tcactctagc catattggta agctacatta tgtcctgatc ttcgattaaa
     5341 caagattgta ctttctctgc gtgtgtgtaa tacgagatgt ttataaaagt ttagcccagc
     5401 aagtggaaca ttttcaaatt tttccagaca gcggccacaa ttcgacgcat ttcacaaatc
     5461 aaacaaaaat cggcaacaaa tgaggaaaaa gatgctgctt ttcatgtgct aaacccgacg
     5521 tttgtgctca cgttgtgtca tgctttgcta atgttcaggt tgtgttttgc gcagcgttaa
     5581 gttggttatt aaactcaaac atgaacaaac aaatataacg atacatgaaa atcgcacaaa
     5641 gaatacatgg tttttgattc gcgggcataa gattgtctag tttgctcaaa agtatttgta
     5701 tttcgggaga taattatatt taaaaaattt tgaaatgtta tggaagtggt cgtttgatat
     5761 caagatcgaa caatttttta aagatatgtg tttcctatta gtatggtgta cccataagaa
     5821 ttgcaatctc taaacaatat ttttaatgat atgaacattt aatgatattc ttcattcatt
     5881 tttgaggatt ccttactaag tcacaaaaaa atcgtcgaat taggtatttt ctagatgcca
     5941 attcgaggtt tcaaaaagtg tcacacacaa gttgaatata ccttaataaa aacaagttac
     6001 ttccaatttg gaacaaacca tcaaaactta gtattgtagt ctcttacagt ctaatagtat
     6061 tccacgaaaa catttccggg aattcgtatg gcacgcaata ctattagaag gtaaatcaat
     6121 tttcataaat tataaaccaa aaaaaaatat tgatggaaaa ttattgtttg aacgtttctg
     6181 tagtctattt tattaattta ttgttttatc tcgtatatga cttgtttaat tgacttgcat
     6241 tactagacag tctcaatttc ctaaaaacga actagtttct aattttttga ataagacttt
     6301 agtttcatgg aaattaaggt ttattggaaa ccaatcacaa agctaacatc acaaatttat
     6361 tttaaatgat atgcgctttg tgttattctc atatttaaat ttcagtggtc ttgctgccgg
     6421 attccttttg ctaaagttgc aaaagcaaag agagaaaatg taccacgtga gtgaaattag
     6481 acacggttga ggtagatcac aagaccaaca gggcttgact tacaggtgtt agatcaaggg
     6541 ttgggtagga atagaaatga agaacatgac agtcatcatt tcaagctgaa taagttattt
     6601 atatcaatca gtttttcgtt tgcagctgca ttatcctttg tacaaattgg taacaaaaaa
     6661 gtatgactaa ttcgaaacca ttcgaaaaat ttccagctac aaaaatgaga tatcttgatc
     6721 tccctgacac tcctgatctg attaacagaa aaatctactt tgtgattctt gaaggatacg
     6781 tggtacggtt gatattcttt atgcttgcca aaaattttca aaattaagat tttcatcgct
     6841 tccagttgca tttcattagt cgcaatatta ttttttcaac tatgtagaat actccaattt
     6901 tcgataggac aactaataga agaaatggtg cctaaagaaa aagaagaatg cccactacca
     6961 gaacaatctt tacaacaaat tcacgatgtt caaattcatt accaagaaat ttccaatgct
     7021 aagctgtata tagaacagaa tttctcattt tcactgtttt atacctacgg ctgttgtatt
     7081 ccgctgacct gtctgttggg ctacattgct tttcgaaatg gaattcaggt agtgaaaatg
     7141 agcagcaata ccattgtttc atatcatcat ttaggctgat atggcggaaa ctttttctgt
     7201 ggcaatatgg ctcactaata ctatgttagc attgatgctt ttctcgattc cagcttttat
     7261 gattgctgaa gaggttagta cttataagta tgtatatgtt tgtttaaata catagatata
     7321 gttagagcag aactgagaaa attgagttag taaagttttt gtatttcttc aacttttgat
     7381 gttagctgaa caaaaattaa aaaacaacat aaattaaata taatgcaagc atatttgtga
     7441 acaagaaatg tgtacgtgga taaagtcgcg gtggaattat catttaaccg tgattgcatg
     7501 catataaatc ccacaatacg taaactcaat ttatttatga cagtttcaca attacgatca
     7561 gaaatttaaa ataaataggt tttcaaacac tattcttcag cgacaatatt tctgctaatt
     7621 gtcgctgata ttttatgttc gcaaacgcag taattcaaaa ttgatatcca aacattaagt
     7681 tgtacaaata aaaattaata attacagggc gacaaacttc taaccgcttc tttcaaaatg
     7741 tatcacgaaa ctctatgtga ggaacgtgat cttctagttt tggtgtgttc gttatctcaa
     7801 aaaaaaatcg atcaattcta ctttcagtct caaatgagtt ttctttcgtt tcaaatgcat
     7861 gctacaaagc ttactctcac cgccgggaat ttcttcatga tgaatcgcaa aattatgatc
     7921 agtgtacgtt taaaggcgtg cagctggttt ttagttgata tttaaaatat ttcagttgtt
     7981 ctctgcaatt ttcacatatt ttcttatttt ggtacagttt gatgctgaaa aggagcgagc
     8041 cggagaatgc aataatcaat ctcgtgtgct cattgtccaa ccacctgtgt aagcgccggt
     8101 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     8161 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     8221 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     8281 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     8341 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     8401 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     8461 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     8521 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     8581 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     8641 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     8701 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     8761 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     8821 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     8881 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac
     8941 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9001 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9061 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9121 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9181 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     9241 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9301 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9361 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9421 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9481 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9541 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9601 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9661 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9721 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9781 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9841 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9901 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9961 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10021 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10081 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10141 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10201 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10261 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10321 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11041 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11101 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11161 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11401 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
    11461 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
    11521 caacgatgga tac
//
