LOCUS       sequence-461146-       11750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing 
            construct into the C. neoformans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11750)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2648
FEATURES             Location/Qualifiers
     source          1..11750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1058..1074
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1059..1075
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2887..2940)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6166..7182)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="PELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVL
                     RVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETE
                     LPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVM
                     DDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDS
                     QYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFD
                     DAAWAQGRCDAIVRSGAGTVGRTQIAHRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(8025..8044)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8026..8044)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8051..8068)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8051..8067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8060..8082)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8208..8663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8295..8314)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8505..8526
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8794..9597)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9598..9813)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9837..9856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9956..9978
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10016..10034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10075..10578
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10615..10719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10720..10788,10789..11580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10938..10957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctcg aggtcgacgg tatcgatcgt
     1081 tcaatctcat agtcttctaa agatcccccg ttcccaaaca tgacattcaa catcttcgct
     1141 tcgacttcca cctcgaccaa ccccttttct tccaaatcca ctcgacgtgc gccatccccc
     1201 tcttcctgcc ccatccctcc tccgtttgcc ccgccaactc ttcccaggtt gatgaagggg
     1261 gtgccctggc ccgacaagcg tcgaggcgag gtaagtgtgt ggctgagatt caaggcatta
     1321 acgaggacgt gtgagcaggt ggaagtgcgg ttgcggtagg aaggacgagg tcagggatgc
     1381 gtagagacat cggtaattct gtacatctgt ttcttgcttc tttccccgtt ctttccatgc
     1441 aacaaactat tattacaaca tgaactctct tagcatgaca gtccacagaa ccgagatggt
     1501 cagtgcatgt cgagattatt tcgtggtgat ctccaggaac gactgaaaaa aggagtgacg
     1561 gattaaggaa gacgtgaccg actaaaggaa aggaaggatg gaaggaacga tatcaatgcg
     1621 atggtggcga cctgagaatg agtaaatagt tgaaagcact aaaataaagc agcaacgaag
     1681 gttattccaa gttatagctc tcgtgcaaat ttcaacagtg atggccattt tattgttgca
     1741 agagacgtcg tcagccgaga aatattcgat ttcgcggaca actatcggga atccaataac
     1801 ggacaaacga catggaatga tatatcacgg caatgaagat tttgaagcgc agaggtatac
     1861 atcagcaaat aagacacaaa acgtaagaag cacaagtagc aacagtcatc cgatgcatat
     1921 tacgcgtaag aagggtcact acataagtaa ttaggaggag gtcttctgct gggtcatcat
     1981 gttctttcgg tagtttcgga cggggggcca accgacgacc tgggccttgg cggggggctt
     2041 ggaggggtcc ttgagggtgg tcttctcctt ggggacggcg gagacgttct tgaggtcgac
     2101 ggaaccctcc ttgttggact ggaggttgag catgaggtcg acggtctcgg agaaaccact
     2161 acctccggat ccagaaccct tgtagagctc gtccataccc atgacgtcgg tgaaggcctt
     2221 ctgccactcc ttgaagttga gctcggtctt ggagtgcttg agctcggtct ttcggaagac
     2281 gtacatgggc tggttcttga ggtagttggc ggccatgggc ttggcgaagg tgtaggtggt
     2341 tcgggcggtg gatcggtatc gcttaccgtt accggtggtg taggaccact tgaaggtgga
     2401 gatgatggtc ttgtcgttgg ggtaggtctt cttggatcga caccagtcgg cggcggtgag
     2461 ggagttggtc atgacgggac cgtcggcggg gaaaccggta cccttgacct gggcctcacc
     2521 cttgatgtgg gaaccctcgt aggtgtatcg gtagttgacg gtgagggagg caccgtcctc
     2581 gaactgcatg gttcggtgga cctggtaacc ggaaccgtcg accatggcgg cctggaaggg
     2641 ggacataccg tcggggtagg ggaggtactg gtggaaaccg taaccgatgt gggggacgag
     2701 gatccagggg gagaactgga ggtcaccctt ggtggacttg aggttgagct cctcgtaacc
     2761 gtcgttgggg ttaccggtac cctgaccgac catgtcgaag tcgacaccgt tgatggaacc
     2821 gaagatgtgg agctcgtggg tggcggggag ggaggccatg ttgtcctcct cacccttgga
     2881 gaccatggga ccggggttct cctcgacgtc accacaggtg aggagggaac ctcgaccctc
     2941 tccgcttcct gatctgagga tcttgacgaa gttgggggcg tcgtctcggg caccggcggt
     3001 ggttcggtag acgtagagct tctcgacctt ggggaggtca ccgtggttct cctccatctc
     3061 gttggaaccg tctcgctcgt tgatgacctc gacgttgagc atgggcatct tggaggcgag
     3121 gacctgacaa cccttgaggg tgacgttaca ggaggacatc cagagggatc gcatggtctc
     3181 gtatcgggcg aagttaccga ggagggcggc gtcaccgaag ggggagtctc ggatctcgag
     3241 ctttcggagg ttcttacaac cgttcatgac gtgcatcata cccttgtcgg agtcaccggc
     3301 gaaggcgatg gagagcatct cgagctgctt ggcgtactta ccgatgtaca tgaagacctt
     3361 gtcggtgagg agaccggaga tggagagtcg ctggagaccc ttacactctc ggacgatggc
     3421 accgaaaccc tcgtcgaggg gctgggaggt gacgacgtcg ggcttaccgg gctcgaggat
     3481 acagagtcgg aatcgggtga agttgggaca gttcttggcg acggtgacga gggcggcgtt
     3541 ggtcatctgg tgacagaagt agaggaggga gttgagcttg ggacaaccga gggagacggc
     3601 gacgagaccc tcctcggtga cggcggagta accggcgacg tagaagtcgg aggggaagac
     3661 tcggagctcc tggaggtcct tacaggagga ggcgacgacc tggagaccct tgtcggagat
     3721 acagtcgagg acccagagtc gctggagctt gacacatcgg gagatcatct tggtgaggtc
     3781 ggaggcgtcg agggtggggg cgtaggagag gttgagaccg gtgagctggg cacagagggg
     3841 gtagatgaag gagagacaga cgggggaggc gtcccagaaa ccggagaggg atcggagcat
     3901 cttacacttc tcgagggcgg aggtgagctt gaagtaggac tcggtctgga agtcgtcggt
     3961 gaggttaccg gtaccgaggt cctcgaggtt gggggttcgg aggaggatct tggcgagggt
     4021 gtcgacggag acggatcggt tgagtcggag ggatcggagg ttgggggatc gggagacgag
     4081 tcgctcgagg gaaccggcgt tgacctcacc cttgatacag gcgaagttga gggagacgag
     4141 ggaggtacag gagtcgggga aacaggagag ccatcgggga cctcggtcct cgacctcgtt
     4201 ctcctggagg tcgagctctc ggaggagctt acagtgctgt cgggagatgt tagtggagta
     4261 acatgttgcg ggaaacgggg ttacatacgg aggcgacggc ggcgagaccg tcggtggaga
     4321 aaccctcaca ggagatgagg acgagggctc ggaatcgggg gaaggatcgg gcgaggagct
     4381 cgagggactc gtcggagacg accattcgct tcattcggag ctcctcgaga ccgtgacaac
     4441 ctcgggcggc ggcctcgatc cagggaccgg cgtaaccacc ccagtcgggg gggacgaggt
     4501 tgaagtcggc accgtggggc ttacccttga cggtgagggc tcggacgttg gggaatcggg
     4561 cggcgactcg accggctcgg acggcgtaac agttaccgac gaagacacct cgtcgggaga
     4621 gtcgctcgat ctcgtaccag accttacaga cgagggagac ggtgtttcgg tctcgctggg
     4681 cggggaggaa ggagaagatg tgctcgacga cctcctcggg gaagtaggtc attttgaagt
     4741 tttctgtgga gatcgttagc atgtgcgaag aggcaatgat aggacaataa agtgttaggg
     4801 cgttgtgagt tggaagggca tgtatccgat gggttgaaaa ttttcaagag ccaccaaatt
     4861 tttttcttca ccacacacaa gcccatgttg agctcagtag tggcaaaaca gtactctgca
     4921 gtcgacgcaa cagatgaggg caaaagcatg gaagacatcg ccaaaaacaa gacagaatga
     4981 tgtgggtggt cgaccatgct cagctccact ctgccccgac ttcacctgca gaagccacca
     5041 gcgagaattg ttttacgtgc cctacgactg gcgcggattt tcaaaggtgt caaaagactt
     5101 tgggtactca cctaggtgtt ttggttaaag aatgtagaga taaagaaggt ggtgggagaa
     5161 aggaatgtgc gagggattta attctttccg gttgatgggc agggcggatt ctcggattaa
     5221 ttcagaaaat cccacaattt ttttcgaaac ctccagaatg tgaaaagctc actggaaatt
     5281 tcatccgtat caaactccgt ttttcaccga gtgtggcaca tgggacttag gaggcaagaa
     5341 atgtgaggag gatggagcga ccgcaacaac ggagaaattt aaagtggagg tcgttcccag
     5401 gcacgtcttc gcaccaaaaa ttcaacgggt ggcaagaggc ccctgccgtc taaacatgtg
     5461 ggagtggctc atcctcacac cgagcaggcc aatgcatatg ataagagtcg tccagaagcc
     5521 gacgtgcatg aatggagctt cacaaaatgc gaaattagaa gaagacgaga tgcccatgat
     5581 cttctgggaa gggggcgagg caacttgctt ggcgaatggg gctgaatatt ggggcgttcg
     5641 ctaatggcca tgacgcttgt tcatggcagg gtagaacacg acattattgc ttgtgcgtgg
     5701 gggcttctgg ctgctttttg tacattgcgc gaagggttgc catgatgaga ctgctgtatt
     5761 atgctcttat tcttttgttt aaagggagtg atacttgaca taccgatgat agatagttaa
     5821 gaagcgcccg caggtgggtc aaccacactt gcgaccattg ttaatcattc agaacggtcg
     5881 acaaggtcag tgtaagatca ttcgctatgg agcttgcata gtagccacaa tcttgtaccg
     5941 tatagcatag aatggccatg aagatcctga ggattcaacg gcgttgctga gggtgggaag
     6001 gatggtattg ttgtctgtag atagattaat gggcatataa tgtcaaaaaa gatgattgac
     6061 acgatgtctg tctcgaattc gcagtgtgct aaaattgtat cacttctcta atatgtatat
     6121 atacaccctc taaggaaaat taacccctta ccgcctcacg aattcctatt cctttgccct
     6181 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     6241 ccagacggcc gcgcttctgt gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     6301 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     6361 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     6421 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     6481 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     6541 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     6601 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     6661 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     6721 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     6781 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     6841 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     6901 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag
     6961 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     7021 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     7081 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     7141 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggtggatcac cttcttcttc
     7201 catagacatg ttgggcgagt ttactaatgg aaaaagacaa ttagcaaaga ggctgcatat
     7261 taggagacgc ggtgaagatt ggggaggaca atagggtccg tgcaccaaaa agtcctgacg
     7321 gcagctctgt tcaacgcaca tccatctctt ccagctatgc gtgtggcacc agcacggatg
     7381 gattgttgca ccaaaaggac aaggcgagat ggatgcaggt cgaggcgatg cggtgctatc
     7441 ggccctcgtt gtcgaacgcc acgtcgtcct ctcggcgacc gtcaatcctg gaaggaaatg
     7501 accaagcgtg cacccagcat ccagcatcca acatccagca cccagcacct agcacccatg
     7561 atccaatggc cggcatgcag atcagcgtgg ggagagcacc atctagcagc ccctggcagg
     7621 cccagcattg tgctcggatg tgcgataggc ggtgggtgcg ataaggtgga ttagtggagg
     7681 atggaggact gagtggcggg caagggaagg gggcgggaag atgactcacc ttgggcagtg
     7741 ggtgggggat gcggtatagg ggatgtatgt ggatgctggc ggaggataga agctgtaagc
     7801 tgagctggtg ccaacggtga cgctgtgaga gtggtctcgg cgaccctgac ggcgactcgg
     7861 cgttaccccg tgtggcacac cggacgcgcg tcgagtgacg ttgctgctat tgttgttacc
     7921 atcatcctct cctccgacaa ccatacactc agtgcacacc atgccactcg aatcctgcat
     7981 gcacagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt
     8041 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8101 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8161 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     8221 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8281 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8341 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8401 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8461 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8521 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8581 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8641 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     8701 atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt
     8761 tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa
     8821 atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt
     8881 tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca catcatccac
     8941 ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat
     9001 aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat
     9061 aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga
     9121 tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac
     9181 ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt
     9241 cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt
     9301 attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa
     9361 tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt
     9421 ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt
     9481 acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt
     9541 ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat
     9601 ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata ctgggcaatt
     9661 tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct
     9721 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     9781 aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca
     9841 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     9901 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     9961 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
    10021 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacggatcgc
    10081 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
    10141 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
    10201 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
    10261 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
    10321 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
    10381 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
    10441 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
    10501 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
    10561 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
    10621 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10681 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10741 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10801 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10861 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10921 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10981 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11041 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11101 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11161 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11221 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11281 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11341 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11401 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11461 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11521 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11581 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11641 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
