LOCUS       sequence-461149-        4840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-5xQUAS+Delta-pes-10P::AI::eTsChR
FEATURES             Location/Qualifiers
     source          1..4840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2302..2324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2424..2443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2521..2539)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2607..2711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2712..2780,2781..3572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2930..2949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3743..4331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4232..4251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4485..4502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4619..4640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4655..4660,4661..4678,4679..4685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4693..4709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4698..4720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4717..4733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4717..4733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gggtaatcgc ttatcctcgg
       61 ataaacaatt atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt
      121 aatcgcttat ccttaagctt gatatccctg caggatcgat tttttgcaaa ttacgagcgt
      181 tgtagggggc ggagcgatag gtcctatagg ttttggtata tcatcattca ttcattcatt
      241 ggtacattca tttacccacc ttcctctttc tgagcttctc tggagttctg tgcttccttt
      301 ttcccttatc tttatactgt aatttttaac tttcagggat ccccgggatt ggccaaagga
      361 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
      421 gagggtaccg agctcagaaa aaatgttcgc catcaacccc gaatatatga atgagactgt
      481 gctgctggac gaatgcaccc caatctacct ggatattgga ccactgtggg agcaggtggt
      541 ggctagggtg actcagtggt tcggagtgat cctgtctctg gtgtttctga tctactacat
      601 ctggaacact tacaaggcta cctgtgggtg ggaggaactg tacgtgtgca cagtggagtt
      661 ctgtaagatc atcatcgaac tgtacttcga gtatactccc cctgccatga tcttccagac
      721 aaatggacaa gtgactccct ggctgcggta tgctgagtgg ctgctgacat gccctgtgat
      781 cctgattcac ctgtctaaca ttactgggct gaatgacgat tacagtggcc gcaccatgag
      841 cctgatcaca tccgacctgg gcggaatttg tatggctgtg accgccgctc tgagcaaggg
      901 atggctgaaa gccctgttct ttgtgatcgg gtgcggctac ggagccagca ccttctacaa
      961 cgccgcttgt atctacattg agtcctacta taccatgcca cagggaattt gcaggagact
     1021 ggtgctgtgg atggccgggg tgttctttac atcctggttc atgtttcctg gcctgttcct
     1081 ggctggacca gaaggaaccc aggccctgtc ttgggctgga accacaatcg gacatacagt
     1141 ggccgacctg ctgagtaaga acgcttgggg aatgatcggg cactttctga gggtggagat
     1201 ccacaagcat atcatcatcc atggagatgt gcggcgcccc gtgacagtga aggctctggg
     1261 gaggcaggtg agcgtgaatt gtttcgtgga caaagaggaa gaggaagagg atgagagaat
     1321 cgcggccgcc aagagcagga tcaccagcga gggcgagtac atccccctgg accagatcga
     1381 catcaacgtg gtgagctaag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     1441 taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat
     1501 atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     1561 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     1621 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     1681 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     1741 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     1801 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     1861 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     1921 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     1981 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     2041 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     2101 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     2161 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     2221 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
     2281 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     2341 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2401 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     2461 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     2521 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2581 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2641 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2701 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2761 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2821 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2881 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2941 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3001 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3061 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3121 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3181 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3241 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3301 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3361 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3421 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3481 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3541 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3601 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3661 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3721 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3781 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3841 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3901 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3961 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4021 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4081 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4141 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4201 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4261 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4321 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4381 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4441 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4501 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4561 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4621 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4681 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     4741 acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca
     4801 gagcttaaaa atggctgaaa tcactcacaa cgatggatac
//
