LOCUS       sequence-46115-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB530
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(36..138)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             245..256
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      121 cctcttacgt gccgatcaac gtctcatttt cgccagatat cgacgtctgt gtggaattgt
      181 gagcggataa caatgatact tagattcaat tgtgagcgga taacaatttc acacagaatt
      241 cattaaagag gagaaattaa ctatgagtag taaagaacag aaaacgcctg aggggcaagc
      301 cccggaagaa attatcatgg atcagcacga agagattgag gcagttgagc cagaagcttc
      361 tgctgagcag gtggatccgc gcgatgaaaa agttgcgaat ctcgaagctc agctggctga
      421 agcccagacc cgtgaacgtg acggcatttt gcgtgtaaaa gccgaaatgg aaaacctgcg
      481 tcgtcgtact gaactggata ttgaaaaagc ccacaaattc gcgctggaga aattcatcaa
      541 cgaattgctg ccggtgattg atagcctgga tcgtgcgctg gaagtggctg ataaagctaa
      601 cccggatatg tctgcgatgg ttgaaggcat tgagctgacg ctgaagtcga tgctggatgt
      661 tgtgcgtaag tttggcgttg aagtgatcgc cgaaactaac gtcccactgg acccgaatgt
      721 gcatcaggcc atcgcaatgg tggaatctga tgacgttgcg ccaggtaacg tactgggcat
      781 tatgcagaag ggttatacgc tgaatggtcg tacgattcgt gcggcgatgg ttactgtagc
      841 gaaagcaaa
//
