LOCUS       sequence-461152-       18401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 18401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1C-CDC73-YY/AA
FEATURES             Location/Qualifiers
     source          1..18401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2733..2756
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2757..2780
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2913..3047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3203..3294
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3340..3363
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3370..3393
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    5119..5253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5409..5500
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7673..7807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7963..8054
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9822..9956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10112..10203
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    11293..11427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  11742..11907
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             12431..13222
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      13263..13717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13350..13369)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13559..13580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13743..13847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13848..13916,13917..14708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14066..14085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14879..15467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15368..15387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15621..15638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  15772..15996
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(16063..16596)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(16785..16790,16791..16807,16808..16813))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(17209..17242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(17288..17307)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    17332..17380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        17475..17566
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 atcctagcaa ccctatggta ttgaaccatt tggcaaatca ctttttcttc aaaaaggatt
       61 atagtaaagt ccagcatctg gccctccatg cattccataa tacagaagtg gaagctatgc
      121 aagcagagag ctgctatcag ctagctagat cattccatgt tcaggaagat tatgaccaag
      181 cttttcagta ctattatcaa gccacacagt ttgcctcatc ctcttttgtg ctcccatttt
      241 ttggtttggg acaaatgtat atttatcgag gtgacaaaga aaatgcatct cagtgctttg
      301 agaaggtttt gaaagcttat cctaataatt acgaaactat gaaaattctc ggctctctct
      361 atgctgcctc agaagatcaa gaaaaacgag atattgccaa gggccatttg aagaaggtca
      421 cagaacagta tcccgatgat gttgaagctt ggattgaatt ggcacaaatc ttagaacaga
      481 ctgatataca gggtgccctt tcagcctatg gaacagcaac acgaatcctt caggagaaag
      541 tgcaggccga tgttcctcca gagattctca ataatgtggg tgccctccat tttagacttg
      601 gaaacctagg ggaggctaag aaatattttt tggcgtcatt ggaccgtgca aaagcagaag
      661 cggaacacga tgagcattac tataacgcca tttccgttac tacgtcatat aatctcgcca
      721 ggctatatga ggcgatgtgt gaattccatg aagcagaaaa actgtataaa aacatcttac
      781 gcgaacatcc taattatgtt gactgctatt tgcgcctagg agccatggct agagataagg
      841 gaaactttta tgaggcttca gattggttta aggaagctct tcagattaat caggatcatc
      901 cagatgcttg gtctttgatt ggcaatcttc atttggcaaa acaagaatgg ggtcctgggc
      961 agaagaagtt tgagaggata ttaaaacagc catccacaca gagtgatacc tattctatgc
     1021 tagcccttgg caacgtgtgg ctccaaactt tacatcagcc cacccgagat cgagaaaagg
     1081 aaaagcgtca tcaagatcgt gctctggcca tctacaaaca agtactcaga aatgatgcaa
     1141 agaatctgta tgctgccaat ggcataggag ctgttttggc ccacaaagga tattttcgtg
     1201 aagctcgtga tgtatttgcc caagtaagag aagcaacagc agatattagt gatgtgtggc
     1261 tgaacttagc acacatctat gtggagcaaa agcagtacat cagcgccgtt cagatgtatg
     1321 aaaactgcct ccgaaagttc tataagcacc aaaacactga agttgtactc tatttggccc
     1381 gggccctctt caagtgtggc aagttacagg aatgcaaaca gactttgctg aaggctagac
     1441 atgtggcacc cagtgataca gttcttatgt ttaatgtggc cttggtcctg caaagattag
     1501 ctacctctgt cctgaaagat gaaaaaagta atctgaagga agtacttaat gctgtgaaag
     1561 aactggagct tgcacataga tacttcagtt atttgagtaa agtgggagat aaaatgagat
     1621 ttgatttggc ccttgctgct acagaagcca ggcagtgttc tgacttactg agccaggccc
     1681 agtaccatgt ggcccgggca cgcaaacaag atgaagaaga gcgggagctg cgggccaagc
     1741 aagagcaaga aaaggagctg ttaaggcaga aacttcttaa agaacaggaa gagaaacgtc
     1801 tcagagaaaa ggaagagcaa aagaaacttt tggaacagcg ggcccagtat gtggagaaga
     1861 ccaaaaatat tcttatgttt actggtgaga ctgaagcaac aaaagagaag aaaagaggtg
     1921 gtggtggtgg acggcgttct aagaagggag gagagtttga tgaatttgtc aatgatgaca
     1981 ctgatgatga cctacctata tccaaaaaga agaagagaag aaagggtagt ggcagtgaac
     2041 aagaaggtga agatgaggag ggtggtgaga gaaagaagaa aaagaggaga agacatccaa
     2101 agggagaaga aggatctgat gatgatgaaa cagaaaatgg ccccaaacca aaaaaacgac
     2161 gtccaccaaa agcagagaag aaaaaggctc ccaagccaga acgtctgcct ccatcaatga
     2221 agggaaaaat aaaatccaaa gccataattt catcaagtga tgactcttcg gatgaggata
     2281 aacttaaaat tgctgatgaa ggacatccca ggaacagcaa cagcaacagt gactcagacg
     2341 aggacgaaca acgaaagaaa tgtgcctcat cagagagtga ttccgatgag aaccagaaca
     2401 agtctggcag cgaggccggc agtccccgga ggccacgaag acagcggtca gatcaggact
     2461 cagacagtga ccagccatcc agaaagagaa ggccctccgg ttctgagcag tctgacaatg
     2521 aatctgtgca gtcagggaga agccactcag gagtttctga gaacgactct cgcccagctt
     2581 ctccaagtgc cgaatcagat cacgaatcgg agagaggatc tgataatgag ggttctggcc
     2641 aaggctctgg aaatgaatcg gaaccagagg gatccaacaa tgaggcctca gatagaggct
     2701 cagaacatgg gtcagatgat agtgacggtg cacttgaagt cctctttcag ggaccctgga
     2761 gccacccgca gttcgaaaag tgaaagcttg tcgagaagta ctagaggatc ataatcagcc
     2821 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2881 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg
     3061 agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     3121 gctgagcaat aactatcata accatttaaa taaactggat actattgcac gtttaaatcg
     3181 acctactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     3241 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     3301 gattattcat accgtcccac catcgggcgc ggatccatgg actacaagga cgacgatgac
     3361 aagctcgatc ttgaagtcct ctttcaggga cccatggcgg acgtgcttag cgtcctgcga
     3421 cagtacaaca tccagaagaa ggagattgtg gtgaagggag acgaagtgat cttcggggag
     3481 ttctcctggc ccaagaatgt gaagaccaac tatgttgttt gggggactgg aaaggaaggc
     3541 caacccagag agtactacac attggattcc attttatttc tacttaataa cgtgcacctt
     3601 tctcatcctg tttatgtccg acgtgcagct actgaaaata ttcctgtggt tagaagacct
     3661 gatcgaaaag atctacttgg atatctcaat ggtgaagcgt caacatcggc aagtatagac
     3721 agaagcgctc ccttagaaat aggtcttcag cgatctactc aagtcaaacg agctgcagat
     3781 gaagttttag cagaagcaaa gaaaccacga attgaggatg aagagtgtgt gcgccttgat
     3841 aaagagagat tggctgcccg tttggagggt cacaaagaag ggattgtaca gactgaacag
     3901 attaggtctt tgtctgaagc tatgtcagtg gaaaaaattg ctgcaatcaa agccaaaatt
     3961 atggctaaga aaagatctac tatcaagact gatctagatg atgacataac tgcccttaaa
     4021 cagaggagtt ttgtggatgc tgaggtagat gtgacccgag atattgtcag cagagagaga
     4081 gtatggagga cacgaacaac tatcttacaa agcacaggaa agaatttttc caagaacatt
     4141 tttgcaattc ttcaatctgt aaaagccaga gaagaagggc gtgcacctga acagcgacct
     4201 gccccaaatg cagcacctgt ggatcccact ttgcgcacca aacagcctat cccagctgcc
     4261 gctaacagag ccgatcagga aagattcaaa ggaaaagaag aaacggaagg cttcaaaatt
     4321 gacactatgg gaacctacca tggtatgaca ctgaaatctg taacggaggg tgcatctgcc
     4381 cggaagactc agactcctgc agcccagcca gtaccaagac cagtttctca agcaagacct
     4441 cccccaaatc agaagaaagg atctcgaaca cccattatca taattcctgc agctaccacc
     4501 tctttaataa ccatgcttaa tgcaaaagac cttctacagg acctgaaatt tgtcccatca
     4561 gatgaaaaga agaaacaagg ttgtcaacga gaaaatgaaa ctctaataca aagaagaaaa
     4621 gaccagatgc aaccaggggg cactgcaatt agtgttacag taccttatag agtagtagac
     4681 cagcccctta aacttatgcc tcaagactgg gaccgcgttg tagccgtttt tgtgcagggt
     4741 cctgcatggc agttcaaagg ttggccatgg cttttgcctg atggatcacc agttgatata
     4801 tttgctaaaa ttaaagcctt ccatctgaag tatgatgaag ttcgtctgga tccaaatgtt
     4861 cagaaatggg atgtaacagt attagaactc agctatcaca aacgtcattt ggatagacca
     4921 gtgttcttac ggttttggga aacattggac aggtacatgg taaagcataa atcgcacttg
     4981 agattctgaa agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
     5041 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5101 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5221 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
     5281 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     5341 atcataacca tttaaataaa cctaatgatg cctgatgttt aaatcgacct actccggaat
     5401 attaatagat catggagata attaaaatga taaccatctc gcaaataaat aagtatttta
     5461 ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt attcataccg
     5521 tcccaccatc gggcgcggat ccatggcgga tatggaggat ctcttcggga gcgacgccga
     5581 cagcgaagct gagcgtaaag attctgattc tggatctgac tcagattctg atcaagagaa
     5641 tgctgcctct ggcagtaatg cctctggaag tgaaagtgat caggatgaaa gaggtgattc
     5701 aggacaacca agtaataagg aactgtttgg agatgacagt gaggacgagg gagcttcaca
     5761 tcatagtggt agtgataatc actctgaaag atcagacaat agatcagaag cttctgagcg
     5821 ttctgaccat gaggacaatg acccctcaga tgtagatcag cacagtggat cagaagcccc
     5881 taatgatgat gaagacgaag gtcatagatc ggatggaggg agccatcatt cagaagcaga
     5941 aggttctgaa aaagcacatt cagatgatga aaaatggggc agagaagata aaagtgacca
     6001 gtcagatgat gaaaagatac aaaattctga tgatgaggag agggcacaag gatctgatga
     6061 agataagctg cagaattctg acgatgatga gaaaatgcag aacacagatg atgaggagag
     6121 gcctcagctt tccgatgatg agagacaaca gctatctgag gaggaaaagg ctaattctga
     6181 tgatgaacgg ccggtagctt ctgataatga tgatgagaaa cagaattctg atgatgaaga
     6241 acaaccacag ctgtctgatg aagagaaaat gcaaaattct gatgatgaaa ggccacaggc
     6301 ctcagatgaa gaacacaggc attcagatga tgaagaggaa caggatcata aatcagaatc
     6361 tgcaagaggc agtgatagtg aagatgaagt tttacgaatg aaacgcaaga atgcgattgc
     6421 atctgattca gaagcggata gtgacactga ggtgccaaaa gataatagtg gaaccatgga
     6481 tttatttgga ggtgcagatg atatctcttc agggagtgat ggagaagaca aaccacctac
     6541 tccaggacag cctgttgatg aaaatggatt gcctcaggat caacaggaag aggagccaat
     6601 tcctgagacc agaatagaag tagaaatacc caaagtaaac actgatttag gaaacgactt
     6661 atattttgtt aaactgccca actttctcag tgtagagccc agaccttttg atcctcagta
     6721 ttatgaagat gaatttgaag atgaagaaat gctggatgaa gaaggtagaa ccaggttaaa
     6781 attaaaggta gaaaatacta taagatggag gatacgccga gatgaagaag gaaatgaaat
     6841 taaagaaagc aatgctcgga tagtcaagtg gtcagatgga agcatgtccc tgcatttagg
     6901 caatgaagtg tttgatgtgt acaaagcccc actgcagggc gaccacaatc atctttttat
     6961 aagacaaggt actggtctac agggacaagc agtctttaaa acgaaactca ccttcagacc
     7021 tcactctacg gacagtgcca cacatagaaa gatgactctg tcacttgcag ataggtgttc
     7081 aaagacacag aagattagaa tcttaccaat ggctggtcgt gatcctgaat gccaacgcac
     7141 agaaatgatt aagaaagaag aagaacgttt gagggcttcc atacgtaggg aatctcagca
     7201 gcgccgaatg agagagaaac agcaccagcg ggggctgagc gccagttacc tggaacctga
     7261 tcgatacgat gaggaggagg aaggcgagga gtccatcagc ttggctgcca ttaaaaaccg
     7321 atataaaggg ggcattcgag aggaacgagc cagaatctat tcatcagaca gtgatgaggg
     7381 atcagaagaa gataaagctc aaagattact caaagcaaag aaacttacca gtgatgagga
     7441 aggtgaacct tccggaaaga gaaaagcaga agatgatgat aaagcaaata aaaagcataa
     7501 gaagtatgtg atcagcgatg aagaggaaga agatgatgat tgaaagcttg tcgagaagta
     7561 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     7621 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     7681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7801 ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag
     7861 ctgagttggc tgctgccacc gctgagcaat aactatcata accatttaaa taaacggttc
     7921 acatagctta gtttaaatcg acctactccg gaatattaat agatcatgga gataattaaa
     7981 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     8041 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatccatgg
     8101 cgcccaccat ccagacccag gcccagcggg aggatggcca caggcccaat tcccaccgga
     8161 ctctgcctga gaggtctgga gtggtctgcc gagtcaagta ctgcaatagc ctccctgata
     8221 tccccttcga ccccaagttc atcacctacc ccttcgacca gaacaggttc gtccagtaca
     8281 aagccacttc cttggagaaa cagcacaaac atgacctcct gactgagcca gacctggggg
     8341 tcaccatcga tctcatcaat cctgacacct accgcatcga ccccaatgtt cttctagatc
     8401 cagctgatga gaaacttttg gaagaggaga ttcaggcccc caccagctcc aagagatccc
     8461 agcagcacgc gaaggtggtg ccatggatgc gaaagacaga gtacatctcc actgagttca
     8521 accgttatgg catctccaat gagaagcctg aggtcaagat tggggtttct gtgaagcagc
     8581 agtttaccga ggaagaaata tacaaagaca gggatagcca gatcacagcc attgagaaga
     8641 cttttgagga tgcccagaaa tcaatctcac agcattacag caaaccccga gtcacaccgg
     8701 tggaggtcat gcctgtcttc ccagacttta agatgtggat caatccatgt gctcaggtga
     8761 tctttgactc agacccagcc cccaaggaca cgagtggtgc agctgcgttg gagatgatgt
     8821 ctcaggccat gattaggggc atgatggatg aggaagggaa ccagtttgtg gcctatttcc
     8881 tgcctgtaga agagacgttg aagaaacgaa agcgggacca ggaggaggag atggactatg
     8941 caccagatga tgtgtatgac tacaaaattg ctcgggagta caactggaac gtgaagaaca
     9001 aagctagcaa gggctatgag gaaaactact tcttcatctt ccgagagggt gacggggttt
     9061 actacaatga gttggaaacc agggtccgcc ttagtaagcg ccgggccaag gctggggttc
     9121 agtcaggcac caacgccctg cttgtggtca aacatcggga catgaatgag aaggaactgg
     9181 aagctcagga ggcacggaag gcccagctag aaaaccacga accggaggag gaagaggaag
     9241 aggagatgga gacagaagag aaagaagctg ggggctcaga tgaggagcag gagaagggca
     9301 gcagcagtga gaaggagggc agtgaagatg agcactcggg cagcgagagt gaacgggagg
     9361 aaggtgacag ggacgaggcc agtgacaaga gtggcagtgg tgaggacgag agcagcgagg
     9421 atgaggcccg ggctgcccgt gacaaagagg agatctttgg cagtgatgct gattctgagg
     9481 acgatgccga ctctgatgat gaggacagag gacaggccca aggtggcagt gacaatgatt
     9541 cagacagcgg cagcaatggg ggtggccagc ggagccggag ccacagccgc agcgccagtc
     9601 ccttccccag tggcagcgag cactcggccc aggaggatgg cagtgaagct gcagcttctg
     9661 attccagtga agctgatagt gacagtgact gaaagcttgt cgagaagtac tagaggatca
     9721 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     9781 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     9841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     9961 cactgcttga gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
    10021 gctgccaccg ctgagcaata actatcataa ccatttaaat aaacactgac attgacttgg
    10081 tttaaatcga cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat
    10141 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
    10201 aatattccgg attattcata ccgtcccacc atcgggcgcg gatccatgac caaccagtac
    10261 ggtattctct tcaaacaaga gcaagcccat gatgatgcca tttggtcagt tgcttggggg
    10321 acaaacaaga aggaaaactc tgagacagtg gtcacaggct ccctagatga cctggtgaag
    10381 gtctggaaat ggcgtgatga gaggctggac ctacagtgga gtctggaggg acatcagctg
    10441 ggagtggtgt ctgtggacat cagccacacc ctgcccattg ctgcatccag ctctcttgat
    10501 gctcatattc gtctttggga cttggaaaat ggcaaacaga taaagtccat agatgcagga
    10561 cctgtggatg cctggacttt ggccttttct cctgattccc agtatctggc cacaggaact
    10621 catgtcggga aagtgaacat ttttggtgtg gaaagtggga aaaaggaata ttctttggac
    10681 acgagaggaa aattcattct tagtattgca tatagtcctg atgggaaata cctagccagt
    10741 ggagccatag atggaatcat caatattttt gatattgcaa ctggaaaact tctgcatacc
    10801 ctggaaggcc atgccatgcc cattcgctcc ttgacctttt ccccggactc ccagctcctt
    10861 gtcactgctt cagatgatgg ctacatcaag atctatgatg tacaacatgc caatttggct
    10921 ggcacgctga gcggccatgc ctcctgggtg ctgaacgttg cattctgtcc tgatgacact
    10981 cactttgttt ccagttcgtc tgacaaaagt gtaaaagttt gggatgttgg aacgaggact
    11041 tgtgttcaca ccttctttga tcaccaggat caggtctggg gagtaaaata caatggaaat
    11101 ggttcaaaaa ttgtgtctgt tggagatgac caggaaattc acatctatga ttgtccaatt
    11161 taaaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
    11221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    11281 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11341 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11401 atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc
    11461 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata
    11521 accatttaaa taaatctata tctcaatcgg ggttacgtcg ataccccacc gagaccccat
    11581 tgggaccaat acgcccgcgt ttcttccttt tccccacccc aacccccaag ttcgggtgaa
    11641 ggcccagggc tcgcagccaa cgtcggggcg gcaagccctg ccatagccac tacgggttaa
    11701 ttaaaaacta tatctcaatc ggggtttaaa caggagatcc gaaccagata agtgaaatct
    11761 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
    11821 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
    11881 agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat
    11941 caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt tctctgtcac
    12001 agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa
    12061 cgcttatttg cagcctgaac tgggaggcag aataaatgat catatcgtca attattacct
    12121 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc
    12181 ttccggtagt caataaaccg gtaagtagcg tatgcgctca cgcaactggt ccagaacctt
    12241 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
    12301 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
    12361 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
    12421 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
    12481 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
    12541 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
    12601 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
    12661 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
    12721 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
    12781 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
    12841 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
    12901 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
    12961 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
    13021 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
    13081 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
    13141 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
    13201 caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgga ttggcgaatg
    13261 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    13321 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    13381 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    13441 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    13501 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tcttaatagt
    13561 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    13621 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    13681 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    13741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    13801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    13861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    13921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    13981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    14041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    14101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    14161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    14221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    14281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    14341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    14401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    14461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    14521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    14581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    14641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    14701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    14761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    14821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    14881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    14941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    15001 gcagagcgca gataccaaat actattcttc tagtgtagcc gtagttaggc caccacttca
    15061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    15121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    15181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    15241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    15301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    15361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    15421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    15481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    15541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    15601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    15661 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagaccag ccgcgtaacc
    15721 tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg
    15781 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    15841 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    15901 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    15961 aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa
    16021 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
    16081 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
    16141 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
    16201 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
    16261 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
    16321 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
    16381 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
    16441 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
    16501 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
    16561 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
    16621 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
    16681 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
    16741 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
    16801 aggcttatgt caactgggtt tgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
    16861 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
    16921 cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
    16981 gcacggatct gcccttgctt caggagatcg gtagacctcg gccgtcgcgg cgcttgccgg
    17041 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
    17101 tgttcgccca ggactctagc tatagttcta gtggttggct acagctttgt ttgtactatc
    17161 aacaggttga actgctgatc aacagatcct ctacgcggcc gcggtaccat aacttcgtat
    17221 agcatacatt atacgaagtt atctgggttt aaacgctcta tggtctaaag tttttatcga
    17281 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
    17341 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
    17401 cagggctggc actctgtcaa gtgaacgctc tatggtctaa agatttaaat cgacctactc
    17461 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
    17521 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
    17581 ataccgtccc accatcgggc gcggatccat gtcgcggggc tccatcgaga ttcccctccg
    17641 ggacactgac gaggtcattg aacttgactt cgatcagtta ccggagggag atgaagttat
    17701 cagtattctg aaacaggaac acacacaact gcacatatgg attgctttgg cgctggaata
    17761 ctacaagcaa ggaaaaacag aagagtttgt aaaattgttg gaagcagcac gtatagatgg
    17821 caatttggac tatagagacc atgaaaaaga ccagatgact tgcttggata cattggcagc
    17881 gtattatgta caacaggctc ggaaagaaaa gaataaggac aataaaaagg atcttattac
    17941 acaggccacc ttgttgtata caatggccga taaaattatt atgtatgatc agaaccattt
    18001 gttgggaaga gcctgcttct gcctacttga gggtgacaaa atggatcaag ctgatgcaca
    18061 gtttcatttt gtactcaatc agtctccaaa taatattcca gcccttcttg gtaaagcttg
    18121 catttccttc aacaagaagg attacagagg agctcttgct tactataaga aagcattgcg
    18181 tactaaccca ggatgtccag cggaagttcg tttaggaatg ggtcattgct ttgtgaaact
    18241 taacaaactg gaaaaagctc gtctggcatt cagcagagcc ctggaactca attccaaatg
    18301 cgtgggagca ttggttggac tggctgttct agaactcaac aataaagagg ctgattccat
    18361 taaaaatggt gtccagcttc tttccagagc ctatactatt g
//
