LOCUS       sequence-461155-       12354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing 
            construct into the C. neoformans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12354)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2649
FEATURES             Location/Qualifiers
     source          1..12354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1058..1074
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1059..1075
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2887..2940)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6161..7807)
                     /codon_start=1
                     /gene="amdS"
                     /product="acetamidase from Aspergillus nidulans"
                     /label=amdS
                     /note="confers ability to process acetamide for selection 
                     in fungal cells"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFA
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     primer_bind     complement(8629..8648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8630..8648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8655..8672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8655..8671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8664..8686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8812..9267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8899..8918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9109..9130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9398..10201)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10202..10417)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10441..10460)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10560..10582
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10620..10638)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10679..11182
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        11219..11323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11324..11392,11393..12184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11542..11561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctcg aggtcgacgg tatcgatcgt
     1081 tcaatctcat agtcttctaa agatcccccg ttcccaaaca tgacattcaa catcttcgct
     1141 tcgacttcca cctcgaccaa ccccttttct tccaaatcca ctcgacgtgc gccatccccc
     1201 tcttcctgcc ccatccctcc tccgtttgcc ccgccaactc ttcccaggtt gatgaagggg
     1261 gtgccctggc ccgacaagcg tcgaggcgag gtaagtgtgt ggctgagatt caaggcatta
     1321 acgaggacgt gtgagcaggt ggaagtgcgg ttgcggtagg aaggacgagg tcagggatgc
     1381 gtagagacat cggtaattct gtacatctgt ttcttgcttc tttccccgtt ctttccatgc
     1441 aacaaactat tattacaaca tgaactctct tagcatgaca gtccacagaa ccgagatggt
     1501 cagtgcatgt cgagattatt tcgtggtgat ctccaggaac gactgaaaaa aggagtgacg
     1561 gattaaggaa gacgtgaccg actaaaggaa aggaaggatg gaaggaacga tatcaatgcg
     1621 atggtggcga cctgagaatg agtaaatagt tgaaagcact aaaataaagc agcaacgaag
     1681 gttattccaa gttatagctc tcgtgcaaat ttcaacagtg atggccattt tattgttgca
     1741 agagacgtcg tcagccgaga aatattcgat ttcgcggaca actatcggga atccaataac
     1801 ggacaaacga catggaatga tatatcacgg caatgaagat tttgaagcgc agaggtatac
     1861 atcagcaaat aagacacaaa acgtaagaag cacaagtagc aacagtcatc cgatgcatat
     1921 tacgcgtaag aagggtcact acataagtaa ttaggaggag gtcttctgct gggtcatcat
     1981 gttctttcgg tagtttcgga cggggggcca accgacgacc tgggccttgg cggggggctt
     2041 ggaggggtcc ttgagggtgg tcttctcctt ggggacggcg gagacgttct tgaggtcgac
     2101 ggaaccctcc ttgttggact ggaggttgag catgaggtcg acggtctcgg agaaaccact
     2161 acctccggat ccagaaccct tgtagagctc gtccataccc atgacgtcgg tgaaggcctt
     2221 ctgccactcc ttgaagttga gctcggtctt ggagtgcttg agctcggtct ttcggaagac
     2281 gtacatgggc tggttcttga ggtagttggc ggccatgggc ttggcgaagg tgtaggtggt
     2341 tcgggcggtg gatcggtatc gcttaccgtt accggtggtg taggaccact tgaaggtgga
     2401 gatgatggtc ttgtcgttgg ggtaggtctt cttggatcga caccagtcgg cggcggtgag
     2461 ggagttggtc atgacgggac cgtcggcggg gaaaccggta cccttgacct gggcctcacc
     2521 cttgatgtgg gaaccctcgt aggtgtatcg gtagttgacg gtgagggagg caccgtcctc
     2581 gaactgcatg gttcggtgga cctggtaacc ggaaccgtcg accatggcgg cctggaaggg
     2641 ggacataccg tcggggtagg ggaggtactg gtggaaaccg taaccgatgt gggggacgag
     2701 gatccagggg gagaactgga ggtcaccctt ggtggacttg aggttgagct cctcgtaacc
     2761 gtcgttgggg ttaccggtac cctgaccgac catgtcgaag tcgacaccgt tgatggaacc
     2821 gaagatgtgg agctcgtggg tggcggggag ggaggccatg ttgtcctcct cacccttgga
     2881 gaccatggga ccggggttct cctcgacgtc accacaggtg aggagggaac ctcgaccctc
     2941 tccgcttcct gatctgagga tcttgacgaa gttgggggcg tcgtctcggg caccggcggt
     3001 ggttcggtag acgtagagct tctcgacctt ggggaggtca ccgtggttct cctccatctc
     3061 gttggaaccg tctcgctcgt tgatgacctc gacgttgagc atgggcatct tggaggcgag
     3121 gacctgacaa cccttgaggg tgacgttaca ggaggacatc cagagggatc gcatggtctc
     3181 gtatcgggcg aagttaccga ggagggcggc gtcaccgaag ggggagtctc ggatctcgag
     3241 ctttcggagg ttcttacaac cgttcatgac gtgcatcata cccttgtcgg agtcaccggc
     3301 gaaggcgatg gagagcatct cgagctgctt ggcgtactta ccgatgtaca tgaagacctt
     3361 gtcggtgagg agaccggaga tggagagtcg ctggagaccc ttacactctc ggacgatggc
     3421 accgaaaccc tcgtcgaggg gctgggaggt gacgacgtcg ggcttaccgg gctcgaggat
     3481 acagagtcgg aatcgggtga agttgggaca gttcttggcg acggtgacga gggcggcgtt
     3541 ggtcatctgg tgacagaagt agaggaggga gttgagcttg ggacaaccga gggagacggc
     3601 gacgagaccc tcctcggtga cggcggagta accggcgacg tagaagtcgg aggggaagac
     3661 tcggagctcc tggaggtcct tacaggagga ggcgacgacc tggagaccct tgtcggagat
     3721 acagtcgagg acccagagtc gctggagctt gacacatcgg gagatcatct tggtgaggtc
     3781 ggaggcgtcg agggtggggg cgtaggagag gttgagaccg gtgagctggg cacagagggg
     3841 gtagatgaag gagagacaga cgggggaggc gtcccagaaa ccggagaggg atcggagcat
     3901 cttacacttc tcgagggcgg aggtgagctt gaagtaggac tcggtctgga agtcgtcggt
     3961 gaggttaccg gtaccgaggt cctcgaggtt gggggttcgg aggaggatct tggcgagggt
     4021 gtcgacggag acggatcggt tgagtcggag ggatcggagg ttgggggatc gggagacgag
     4081 tcgctcgagg gaaccggcgt tgacctcacc cttgatacag gcgaagttga gggagacgag
     4141 ggaggtacag gagtcgggga aacaggagag ccatcgggga cctcggtcct cgacctcgtt
     4201 ctcctggagg tcgagctctc ggaggagctt acagtgctgt cgggagatgt tagtggagta
     4261 acatgttgcg ggaaacgggg ttacatacgg aggcgacggc ggcgagaccg tcggtggaga
     4321 aaccctcaca ggagatgagg acgagggctc ggaatcgggg gaaggatcgg gcgaggagct
     4381 cgagggactc gtcggagacg accattcgct tcattcggag ctcctcgaga ccgtgacaac
     4441 ctcgggcggc ggcctcgatc cagggaccgg cgtaaccacc ccagtcgggg gggacgaggt
     4501 tgaagtcggc accgtggggc ttacccttga cggtgagggc tcggacgttg gggaatcggg
     4561 cggcgactcg accggctcgg acggcgtaac agttaccgac gaagacacct cgtcgggaga
     4621 gtcgctcgat ctcgtaccag accttacaga cgagggagac ggtgtttcgg tctcgctggg
     4681 cggggaggaa ggagaagatg tgctcgacga cctcctcggg gaagtaggtc attttgaagt
     4741 tttctgtgga gatcgttagc atgtgcgaag aggcaatgat aggacaataa agtgttaggg
     4801 cgttgtgagt tggaagggca tgtatccgat gggttgaaaa ttttcaagag ccaccaaatt
     4861 tttttcttca ccacacacaa gcccatgttg agctcagtag tggcaaaaca gtactctgca
     4921 gtcgacgcaa cagatgaggg caaaagcatg gaagacatcg ccaaaaacaa gacagaatga
     4981 tgtgggtggt cgaccatgct cagctccact ctgccccgac ttcacctgca gaagccacca
     5041 gcgagaattg ttttacgtgc cctacgactg gcgcggattt tcaaaggtgt caaaagactt
     5101 tgggtactca cctaggtgtt ttggttaaag aatgtagaga taaagaaggt ggtgggagaa
     5161 aggaatgtgc gagggattta attctttccg gttgatgggc agggcggatt ctcggattaa
     5221 ttcagaaaat cccacaattt ttttcgaaac ctccagaatg tgaaaagctc actggaaatt
     5281 tcatccgtat caaactccgt ttttcaccga gtgtggcaca tgggacttag gaggcaagaa
     5341 atgtgaggag gatggagcga ccgcaacaac ggagaaattt aaagtggagg tcgttcccag
     5401 gcacgtcttc gcaccaaaaa ttcaacgggt ggcaagaggc ccctgccgtc taaacatgtg
     5461 ggagtggctc atcctcacac cgagcaggcc aatgcatatg ataagagtcg tccagaagcc
     5521 gacgtgcatg aatggagctt cacaaaatgc gaaattagaa gaagacgaga tgcccatgat
     5581 cttctgggaa gggggcgagg caacttgctt ggcgaatggg gctgaatatt ggggcgttcg
     5641 ctaatggcca tgacgcttgt tcatggcagg gtagaacacg acattattgc ttgtgcgtgg
     5701 gggcttctgg ctgctttttg tacattgcgc gaagggttgc catgatgaga ctgctgtatt
     5761 atgctcttat tcttttgttt aaagggagtg atacttgaca taccgatgat agatagttaa
     5821 gaagcgcccg caggtgggtc aaccacactt gcgaccattg ttaatcattc agaacggtcg
     5881 acaaggtcag tgtaagatca ttcgctatgg agcttgcata gtagccacaa tcttgtaccg
     5941 tatagcatag aatggccatg aagatcctga ggattcaacg gcgttgctga gggtgggaag
     6001 gatggtattg ttgtctgtag atagattaat gggcatataa tgtcaaaaaa gatgattgac
     6061 acgatgtctg tctcgaattc gcagtgtgct aaaattgtat cacttctcta atatgtatat
     6121 atacaccctc taaggaaaat taacccctta ccgccttcac ctatggagtc accacatttc
     6181 ccagcaactt ccccacttcc tctgcaatcg ccaacgtcct ctcttcactg agtctccgtc
     6241 cgataacctg cactgcaacc ggtgccccat ggtacgcctc cggatcatac tcttcctgca
     6301 cgagggcatc aagctcacta accgccttga aactctcatt cttcttatcg atgttcttat
     6361 ccgcaaaggt aaccggaaca accacgctcg tgaaatccag caggttgatc acagaggcat
     6421 acccatagta ccggaactgg tcatgccgta ccgcagcggt aggcgtaatc ggcgcgatga
     6481 tggcgtccag ttccttcccg gccttttctt cagcctcccg ccatttctca aggtactcca
     6541 tctggtaatt ccacttctgg agatgcgtgt cccagagctc gttcatgtta acagctttga
     6601 tgttcgggtt cagtaggtct ttgatatttg gaatcgccgg ctcgccggat gcactgatat
     6661 cgcgcattac gtcggcgctg ccgtcagccg cgtagatatg ggagatgaga tcgtggccga
     6721 aatcgtgctt gtatggcgtc cacggggtca cggtgtgacc ggctttggcg agtgcggcga
     6781 cggtggtttc cacgccgcgc aggataggag ggtgtggaag gacattgccg tcgaagttgt
     6841 agtagccgat attgagcccg ccgttcttga tcttggaggc aataatgtcc gactcggact
     6901 ggcgccaggg catggggatg accttggagt cgtatttcca tggctcctga ccgaggacgg
     6961 atttggcgaa gaggcggagg tcctcaacag agtgcgtaat cggcccgaca acgctgtgca
     7021 ccgtctcctg accctccatg ctgttcgcca tctttgcata cggcagccgc ccatgactcg
     7081 gccttagacc gtacaggaag ttgaacgcgg ccggcactcg aatcgagcca ccgatatccg
     7141 ttcctacacc gatgacgcca ccacgaatcc caacgatcgc accctcacca ccagaactgc
     7201 cgccgcacga ccagttcttg ttgcgtgggt tgacggtgcg cccgatgatg ttgttgactg
     7261 tctcgcagac catcagggtc tgcgggacag aggtcttgac gtagaagacg gcaccggctt
     7321 tgcggagcat ggttgtcaga accgagtccc cttcgtcgta cttgtttagc catgagatgt
     7381 agcccattga tgtttcgtag cccttgactc gaagctggtc tttgagagag atggggaggc
     7441 catggagtgg accaacgggt ctcttgtgct ttgcgtagta ttcatcgagt tcccttgcct
     7501 gcgcgagagc ggcgtcaggg aagaactcgt gggcgcagtt tgttaactgc tgggcgattg
     7561 ctgcccgttt acagaatgct agcgtaactt ccaccgaggt caactctccg gccgccagct
     7621 tggacacaag atctgcagcg gaggcctctg tgatcttcag ttcggcctct gaaaggatcc
     7681 ccgatttctt tgggaaatca ataacgctgt cttccgcagg cagcgtctgg actttccatt
     7741 catcagggat ggtttttgcg aggcgggcgc gcttatcagc ggccagttct tcccaggatt
     7801 gaggcataga catgttgggc gagtttacta atggaaaaag acaattagca aagaggctgc
     7861 atattaggag acgcggtgaa gattggggag gacaataggg tccgtgcacc aaaaagtcct
     7921 gacggcagct ctgttcaacg cacatccatc tcttccagct atgcgtgtgg caccagcacg
     7981 gatggattgt tgcaccaaaa ggacaaggcg agatggatgc aggtcgaggc gatgcggtgc
     8041 tatcggccct cgttgtcgaa cgccacgtcg tcctctcggc gaccgtcaat cctggaagga
     8101 aatgaccaag cgtgcaccca gcatccagca tccaacatcc agcacccagc acctagcacc
     8161 catgatccaa tggccggcat gcagatcagc gtggggagag caccatctag cagcccctgg
     8221 caggcccagc attgtgctcg gatgtgcgat aggcggtggg tgcgataagg tggattagtg
     8281 gaggatggag gactgagtgg cgggcaaggg aagggggcgg gaagatgact caccttgggc
     8341 agtgggtggg ggatgcggta taggggatgt atgtggatgc tggcggagga tagaagctgt
     8401 aagctgagct ggtgccaacg gtgacgctgt gagagtggtc tcggcgaccc tgacggcgac
     8461 tcggcgttac cccgtgtggc acaccggacg cgcgtcgagt gacgttgctg ctattgttgt
     8521 taccatcatc ctctcctccg acaaccatac actcagtgca caccatgcca ctcgaatcct
     8581 gcatgcacag ttctagagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     8641 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8701 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8761 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     8821 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8881 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8941 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9001 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9061 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9121 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9181 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9241 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     9301 cggtatttca caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg
     9361 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc
     9421 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc
     9481 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat
     9541 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg
     9601 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc
     9661 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag
     9721 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg
     9781 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg
     9841 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga
     9901 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg
     9961 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat
    10021 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg
    10081 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata
    10141 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca
    10201 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc
    10261 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
    10321 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa
    10381 atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag gtggtatggt gcactctcag
    10441 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
    10501 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
    10561 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
    10621 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga
    10681 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
    10741 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
    10801 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
    10861 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
    10921 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
    10981 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
    11041 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
    11101 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
    11161 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
    11221 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    11281 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    11341 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    11401 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11461 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11521 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    11581 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    11641 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11701 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11761 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11821 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11881 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11941 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    12001 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    12061 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    12121 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    12181 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    12241 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    12301 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
