LOCUS       sequence-461156-       10352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK9/Cyclin T1
FEATURES             Location/Qualifiers
     source          1..10352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(124..141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(295..883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(375..394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1054..1845,1846..1914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1677..1696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1915..2019)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2045..2499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2182..2203)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2393..2412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2540..3331)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  3855..4020
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    4335..4469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6822..6913)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7069..7203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8449..8472)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(8479..8502)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8548..8639)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8734..8782
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8807..8826
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    8872..8905
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(9301..9306,9307..9323,9324..9329)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9518..10051
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  10118..10342
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg gctggtctat
       61 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
      121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      721 cggctacact agaagaatag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2041 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2101 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2161 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaag aacgtggact
     2221 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2281 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2341 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2401 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2461 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccgtcggc
     2521 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     2581 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     2641 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     2701 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     2761 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     2821 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     2881 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     2941 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     3001 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     3061 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     3121 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     3181 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     3241 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     3301 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc
     3361 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3421 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca
     3481 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3541 gtgagcgcat acgctactta ccggtttatt gactaccgga agcagtgtga ccgtgtgctt
     3601 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     3661 gatcatttat tctgcctccc agttcaggct gcaaataagc gttgatattc agtcaattac
     3721 aaacattaat aacgaagaga tgacagaaaa attttcattc tgtgacagag aaaaagtagc
     3781 cgaagatgac ggtttgtcac atggagttgg caggatgttt gattaaaaac ataacaggaa
     3841 gaaaaatgcc ccgctgtggg cggacaaaat agttgggaac tgggaggggt ggaaatggag
     3901 tttttaagga ttatttaggg aagagtgaca aaatagatgg gaactgggtg tagcgtcgta
     3961 agctaatacg aaaattaaaa atgacaaaat agtttggaac tagatttcac ttatctggtt
     4021 cggatctcct gtttaaacca agtcaatgtc agtgttttta attaacccgt agtggctatg
     4081 gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt
     4141 tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta
     4201 tcgacgtaac cccgattgag atatagattt atttaaatgg ttatgatagt tattgctcag
     4261 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tcttctaggc
     4321 tcaagcagtg atcagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     4381 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4441 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4501 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4561 tggctgatta tgatcctcta gtacttctcg acaagctttt acttaggaag gggtggaagt
     4621 ggtggaggag gttctgatgg cagaggtggt ggccggggtt tgtctgtatt gccagatctc
     4681 gaggagattc caccagaccg aggattcaga tagtcactat aaggacgaac aaattcaaat
     4741 gcagtaggct gagtgggctg aacaccctgg gcactgagca gggagtgaag catattcaca
     4801 gtgtcttgat agtctattgt ctgggtcgtg ttgtgaccat tggccccagt gggcccttta
     4861 tccagtttcg aatgagggac acgagtttta cagctgggct gtgaaaagga aaggccactt
     4921 gtgtctgagc tatgcccagg catctgaccc attgtaggaa gtgaagggaa ggagaaattt
     4981 agggaagagg atttagtact cttggcaatc ttggctggat gatcaaacac agcccctcca
     5041 gtctcttcag agggtcccct tttacgagta gaactggaag aggaaaaaga actagacaag
     5101 ctataggttt tatgtgctaa gttgcttgtc tggctactat gttttggatc accaggacgt
     5161 ttgttcccag taccaactgg aagttgggaa tgagagtgct tgtgtgagtg gtgattatga
     5221 tgatgatgat gattagatgg gtgagtcttg tgcttttctt tgtgctctcg gctctttgta
     5281 acactgtcct ctacagaatt gtgcttatca gctgcagcat ggacttttat gcgcattttt
     5341 atctcctctg gttttgaaga cgcagcttta tctccacctg ccactgggat tctcattttg
     5401 agagctgttt tgtcagcctt ttccagaaaa ggccgctcgg ggttttctga accctctatg
     5461 ggcattttta gaatgactga agaatggcta tcatgatgag aaaggagatt ctgggcagca
     5521 tatgcatatt gtgacttcac attggcttcc atgttctcca gttgcctctt ctgggcagcc
     5581 aattcttctg catgcttcgc gcggtattct ttcagtgaca ctttagctga tggcacactc
     5641 ttactattct gcttctggga aataaatgca tttgaaccat cctgtggtaa ggaatgatca
     5701 actcctgtaa gtgctaaatt ctcactagtc cgatgaccct gagtaggttc tagtttgaaa
     5761 gaaggttggg aggacagcca acgcttgccc ggcaacatct ccacactggt taagttgctg
     5821 gatgactctt cggagactgg cagggaaggc actgcacttg tggtagaagt tgacatgctc
     5881 attaaacctg caatggttgt gtctgaagag ctctgggaaa tcatattgag gattgtctgc
     5941 tctgaagtct tttcatctgt tcctcggtca tctgcttttg ttttcttggc agcctcgcat
     6001 gccctccaat tccaaatgcg tttgagcctg ttgggagttt tctccaaaat ctgtagaaac
     6061 tcatgtgtca gttcatctaa aagttccaag gtcacagtgg cgtcaacata ctcccaccag
     6121 tgcttcccgt cagttgagac tgggatctcc caattggacc acttgcaagc caggtgaatg
     6181 cagacacagg ccaccacagg aggtgtgtac tgcaggctaa atgtggtcaa atgcaggctg
     6241 ttggttgcca tgaagtaaga agtctgtgct aagtccttgc ttgctcgaac aagttgagtg
     6301 cactttacta catgagtatg tgggtgatca attgttagtt caaagcctaa agtctgcaaa
     6361 attatgcttt ctaaaatgac cagatcttga acttgttgca aataagcctc acttctagta
     6421 tcaggaaggg attcctgagg atggagacaa gtatgtgcta ccttgatgac atgttccaat
     6481 tttttgggct gctcctccac tttagctgct agaaacaagg ctgctggagc cacagaattt
     6541 ccagggaact gtgtgaagga ctgaatcatg tagaatcgat gcatgtatac tatagcagtg
     6601 ttgatagtca attgtgagac gttaagacgc tgccccatgt cctgaagcag attggccgcc
     6661 tgctggcgat aagaaagttc tttatctggg tccacgccaa aacgacggga tgggctattt
     6721 tccagctgtt ctcgagtgaa ataccaccgt ttgttgttgt tcttcctctc tccctccatg
     6781 gatccgcgcc cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt
     6841 acaaaactgt tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa
     6901 ttatctccat gatctattaa tattccggag taggtcgatt taaacgtgca atagtatcca
     6961 gtttatttaa atggttatga tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
     7021 tttcgggctt tgttagcagc cggatcttct aggctcaagc agtgatcaga tccagacatg
     7081 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     7141 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     7201 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     7261 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt
     7321 ctcgacaagc tttcagaaga cgcgctcaaa ctccgtctgg ttggtggtgg cgggattgcg
     7381 actctggttg gtggactgct gggtgatctg gctgcccttc cggcgcggtg gtgccaagta
     7441 ctcgaacatg gacgtcaggt gggtggagag catgcccttg aggtcggagg gcatggggtc
     7501 ggaccagaag aagtcgtggt tgagggcgtc atcgctgtcg atgcgctggg cagggtccag
     7561 caccagcagc ttgtcgatga ggtccagtgc gtatgggtca cgcacatagg ccttcagcct
     7621 gtccttcacc ttccgcttct ggcccttgac cagctccagc ttttcgtaca gctcatagtt
     7681 gtccacgttt ggccacacct caggggtgat ggagccgcag agctgactga tgagggcgag
     7741 ttggtgctgc tccgtgttgc cctgcatgat ggggctgcgg gtccacatct ctgccatgat
     7801 gcacccagca ccccacaggt caatgggggg gccgtagtcc cgctccccga gcaacagctc
     7861 cgggggccgg taccagagtg tcaccacacg gttggtgtag cggttgggct ggctgttctt
     7921 ggccaggctg aaggcccggg ccagcccaaa gtctgccagc ttcaggaccc catcacgagt
     7981 gataagcaca ttagcagcct tcatgtccct atgcaggatc ttgtttctgt ggatgtagta
     8041 gaggccgtta agcagcatct gcatcaccct cttgatctca gacagcgtga acttgaccaa
     8101 aacattgctc aacagcccag caaggtcatg ctcgcagaag tcgaacacca ggtatatact
     8161 acccttgcag cggttatagg gggaagcttt ggttcgacaa atctcaatca agttgaccac
     8221 attctcgtgt tttagaagct gaaggatctt gatctcccgc aaggctgtaa tggggaaccc
     8281 ctccttctcg ttttccatca gcaccttctt cagagccacc ttctggccgg tcttgcggtg
     8341 cctggccttg aacacctccc cgaaggtgcc ttggccgatc ttggcgagct tctcgtattt
     8401 ggaaacttca tcacaaaaag ggcactccac cgagtcgtac tgctttgcgg gtccctgaaa
     8461 gaggacttca agatcgagct tgtcatcgtc gtccttgtag tccatggatc cgcgcccgat
     8521 ggtgggacgg tatgaataat ccggaatatt tataggtttt tttattacaa aactgttacg
     8581 aaaacagtaa aatacttatt tatttgcgag atggttatca ttttaattat ctccatgatc
     8641 tattaatatt ccggagtagg tcgatttaaa tctttagacc atagagcgtt cacttgacag
     8701 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt
     8761 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
     8821 gtttcagtta gcctcgataa aaactaagct atgtgaaccg tttaaaccca gataacttcg
     8881 tataatgtat gctatacgaa gttatggtac cgcggccgcg tagaggatct gttgatcagc
     8941 agttcaacct gttgatagta caaacaaagc tgtagccaac cactagaact atagctagag
     9001 tcctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc
     9061 ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc taccgatctc ctgaagcaag
     9121 ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga
     9181 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg
     9241 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acaaacccag
     9301 ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac
     9361 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     9421 tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     9481 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt
     9541 tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg
     9601 cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg
     9661 tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg
     9721 gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt
     9781 tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat
     9841 ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa
     9901 tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta
     9961 cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca
    10021 ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc
    10081 ggccgcggag ttgttcggta aattgtcaca acgccgcgaa tatagtcttt accatgccct
    10141 tggccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct
    10201 ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta
    10261 caactattct gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt
    10321 aagactttat tgtccgccca cacccgctta cg
//
