LOCUS       sequence-46116-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB540
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             396..425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    585..601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    618..634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             653..664
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      121 acctcttacg tgccgatcaa cgtctcattt tcgccagata tcgacgtctg tgtggaattg
      181 tgagcggata acaaattcac acagggccct cggacaccga ggagaatgtc aagaggcgaa
      241 cacacaacgt cttggagcgc cagaggagga acgagctaaa acggagcttt tttgccctgc
      301 gtgaccagat cccggagttg gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa
      361 aagccacagc atacatcctg tccgtccaag cagaggagca aaagctcatt tctgaagagg
      421 acttgttgcg gaaacgacga gaacagttga aacacaaact tgaacagcta cggaactctt
      481 gtgcgtaagg aaaagtaagg aaaacgattc cttctaacag aaatgtcctg agcaatcacc
      541 tatgaactgt cgactcgaga aaatttatca aaaagagtgt tgacttgtga gcggataaca
      601 atgatactta gattcaattg tgagcggata acaatttcac acagaattca ttaaagagga
      661 gaaattaact atgagtagta aagaacagaa aacgcctgag gggcaagccc cggaagaaat
      721 tatcatggat cagcacgaag agattgaggc agttgagcca gaagcttctg ctgagcaggt
      781 ggatccgcgc gatgaaaaag ttgcgaatct cgaagctcag ctggctgaag cccagacccg
      841 tgaacgtgac ggcattttgc gtgtaaaagc cgaaatgg
//
