LOCUS       sequence-461164-       13154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1e-CDK13/Cyclin K
FEATURES             Location/Qualifiers
     source          1..13154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  20..244
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(378..395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(549..1137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(629..648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1308..2099,2100..2168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1931..1950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2169..2273)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2299..2753)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2436..2457)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2647..2666
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2794..3585)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     mobile_element  4109..4274
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    4589..4723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6638..6729)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6885..7019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8957..8968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(11505..11528)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(11535..11558)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(11604..11695)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    11790..11838
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     11863..11882
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    11928..11961
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(12357..12362,12363..12379,12380..12385)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             12574..13107
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 taaattgtca caacgccgcg aatatagtct ttaccatgcc cttggccacg cccctcttta
       61 atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc
      121 cagtatgctt tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt
      181 taagacttta ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc
      241 cacacccgct tacgcagggc atccatttat tactcaaccg taaccgattt tgccaggtta
      301 cgcggctggt ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
      361 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      961 tggtggccta actacggcta cactagaaga atagtatttg gtatctgcgc tctgctgaag
     1021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2281 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2461 taagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2521 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2581 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2641 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2701 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     2761 gccaatccgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc
     2821 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     2881 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     2941 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     3001 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     3061 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     3121 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     3181 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     3241 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     3301 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     3361 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     3421 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     3481 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     3541 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
     3601 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     3661 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     3721 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     3781 tctggaccag ttgcgtgagc gcatacgcta cttaccggtt tattgactac cggaagcagt
     3841 gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
     3901 ataattgacg atatgatcat ttattctgcc tcccagttca ggctgcaaat aagcgttgat
     3961 attcagtcaa ttacaaacat taataacgaa gagatgacag aaaaattttc attctgtgac
     4021 agagaaaaag tagccgaaga tgacggtttg tcacatggag ttggcaggat gtttgattaa
     4081 aaacataaca ggaagaaaaa tgccccgctg tgggcggaca aaatagttgg gaactgggag
     4141 gggtggaaat ggagttttta aggattattt agggaagagt gacaaaatag atgggaactg
     4201 ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca aaatagtttg gaactagatt
     4261 tcacttatct ggttcggatc tcctgtttaa accaagtcaa tgtcagtgtt tttaattaac
     4321 ccgtagtggc tatggcaggg cttgccgccc cgacgttggc tgcgagccct gggccttcac
     4381 ccgaacttgg gggttggggt ggggaaaagg aagaaacgcg ggcgtattgg tcccaatggg
     4441 gtctcggtgg ggtatcgacg taaccccgat tgagatatag atttatttaa atggttatga
     4501 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     4561 cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca ttgatgagtt
     4621 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4681 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4741 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4801 ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc ttttatctca
     4861 tccaggctgc ccgccccagc cccccaactg gaggcatgcc gggtggggga atgggcgggg
     4921 gcacaggagg ctgtcctcca ggggggacgg caggaggtgg gtagctggct ggcggcaggc
     4981 ccaaccctgg aggaggatga ggggggactg cgtgggtagg cgggaggcgt gggggtgggg
     5041 gtgggaagtt ggggttgtag gtgggtgggg gtggggggta gccaggagta gggggtggga
     5101 tggcaggtgg ggggaaggag gctgggggag gaggcacagg tggcgggggc gggttgggcg
     5161 ggtagacatg ggggtggtag ggcaggtgtg caggtgggcc gtaggcgggg ggcagggcac
     5221 cgtaggaggg tccctcggtc ttcatcatgg actgcaggct ctggtagccc tctccagaca
     5281 tgtaggagct ggtggtggac atcccggtca tgtagctgga ggggggtggg ggcgggggcc
     5341 ggtgtggcag cggcggtggc tggtgcacag gggccgggtg ggccggaggg ggaatctgga
     5401 ctttggggag gtcagtggca tccacagggc ccggcggctc agcctcacct aaggcagcag
     5461 cgaggggagg cttccggtct ggaggtgggt gggctggagg caacggtgga tgagtggtct
     5521 caattttggg gattttaggt ggtggtggtt ctgctgcttt gttctcttct ttgggagaaa
     5581 caaccacggc tcgcttaacc tgtcggggag aactgggctg cggagagggt ttcttgggct
     5641 gttgggctgg ctgctgctgc tgggctggtt gctgggggtc cttctgctgg ggctgggatg
     5701 gttcggagct ttgagacggc tgtgactgct gtacttgcgg cacttgtggt gtaggctgaa
     5761 gagatggggg ctgttgcagc tgatgggggg tgtgatgagg catctgttgt tttccttgtg
     5821 agtaaagatc caggatttgg tggcagatgt cttccaaaac gtcgaccggg acatcttgaa
     5881 caaactgctc ccaccatctc ctatacatgg gtttggaggt ccattcttgt atttcaaatt
     5941 tgcacaaacg tcctgcgaga tacatcactg ctactgctat gatctctggt tcccactgca
     6001 gtgacaaggt ggtgcagaga ctgtcattta caaatgtcca tgccatttga accaactttt
     6061 gaattttgtt tttatcacct ttgagttgct ttgcatattt tagtaggaac tggtatggat
     6121 gttctacctg taaatcaaac ttgatggtct gcagtaagat tctctccaga accattactt
     6181 cctcctttgg gtcatctcca aactggccaa attgtacatc atttaataaa ctacgagctg
     6241 ttttgatgat atctttacat ttttttggtg tttcttctac tttcccagcc agaaagaggc
     6301 aacaggctcc tgtcacatat cttgggaatt gcttgaagga atgaaacata tagaagcgat
     6361 gaaaataaat tattccagtt gccagggtat catagtgtag ccccaaacgt gtgcccacat
     6421 caaagatgaa ccgagcgccc tctcggcggt accgggcctc ggtggctgga tcaagtcctt
     6481 caagttgtga gggtgtatga gccaagtctt tcttatccca gtaccaacat ggctttgtgt
     6541 ggtccaggtt tgctgaagtt actgaagggc ttgaattttc tttattctcc ttcatggatc
     6601 cgcgcccgat ggtgggacgg tatgaataat ccggaatatt tataggtttt tttattacaa
     6661 aactgttacg aaaacagtaa aatacttatt tatttgcgag atggttatca ttttaattat
     6721 ctccatgatc tattaatatt ccggagtagg tcgatttaaa cgtgcaatag tatccagttt
     6781 atttaaatgg ttatgatagt tattgctcag cggtggcagc agccaactca gcttcctttc
     6841 gggctttgtt agcagccgga tcttctaggc tcaagcagtg atcagatcca gacatgataa
     6901 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6961 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     7021 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     7081 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gtacttctcg
     7141 acaagctttc agtatggtaa ccctctgcct ctgcctctgc cagttgatgt gctaatatgt
     7201 cccctgtatc cttgtgaata tccaggtcct tgggcaggag aagtccaggt ctgtccatgc
     7261 atgaggctgg ggccactcgg attttcctgt aagttaccac catagttata gttgtgcatc
     7321 tttgctggca aaggatgagt actctctggc cccgtggaag ggtcactgct gtttgccagg
     7381 actgcaatag gaccatggct atattcaaat ctatggtcct tgtctccact aaacaacatg
     7441 ggaccagcat tgaggtaaat gtctccatat ccagctactg aactgggaaa tgactcagaa
     7501 aaggcagaag gctgaggtgg accaccagaa tgagatgaag cagtactgta gttatccaaa
     7561 gactgaatat ctgaatttcc aatgaaacta cgtcgttcta gtggtggagc agaactgctt
     7621 cctagaccat cattgctaac ataaggagca gaagagaaag aagaggatcc aaagttgtcc
     7681 ttgtagtctg aagtggacac gtaagtgtct cctgcttgat aatcaatccc accttctctt
     7741 ttggggtcat cctggggctg atgctgagca agcagctgta acagggctgc cttcactccg
     7801 gcatgagggt cagttaatga agaactggtg gtgggtacat gctggttttc tgtccgataa
     7861 tctagatctt cctcagtgac tggtggtggt tcaggaggct cgggaggtcg ttggtcagga
     7921 ggcagaattc gaggccggtc tggttctggc gttagctcca agatcctcat atcttgatgc
     7981 tggatgaggt catcttgtcc cgacaccgga gtgctaggtt ctggaggtgg cctgagttgt
     8041 aatgacgctt catgtcccga tccattttcc ctctcttcta gtagcacatc tttctgcttt
     8101 gactgctgag cctttattaa ctgagtcaac agaactgcaa atgcactctg cacagccgcc
     8161 tgggcagcat cagtctccac tttaggctgg atggtctgag aagaaggctg aattcctccc
     8221 aacggtttcg aagactcctg ttgtggtgtt gatggatctg tctgtttttc accaggtgcc
     8281 acatttgagc tgccctgaga tttcagctgt gaagatggca gcacaccctg gggtgtgttg
     8341 gttctgctgt catccaagcc cagagacaag tccttcctgg gggctttaat tgtggaaaca
     8401 tcatcagtca tgcccatctg cttctgtctt cttcgctttt tactccataa ctcatgacaa
     8461 tcttgccata aagggagatc tggtggaggc atttttgagg gttccacatc tcggaggaac
     8521 tcgcactgaa gagcctgttc agcagtgcag cgcttactag gatccaaggc aagcatgtaa
     8581 tcaaataagt ctagcgcagc tgcaggaata aaaacaaatt cttctcttaa ctttcgacga
     8641 tattgcttct ttggtttcat ggtgttgaaa tatggtagtt tgattacatc aggccacact
     8701 gcaggacatg gactcccaca tattcggctt attaattcta gttgtgcaag ttcctgattt
     8761 gcttgaaata taggtttttt agtgaagagt tcgccaagga tacagccaca gctccataca
     8821 tcaatggctg gtgtgtatcg ttcttctccc agtagcagtt caggtggacg gtaccataaa
     8881 gtaattacct tgttagtata cggccgactt tcttctgagc tatacaatcg agcaagtcca
     8941 aagtctgcaa gttttatctg ccctctatta tttagaagga tattggaaca tttaatatct
     9001 ctatgcaaaa agttcttctt atgacaataa tccagaccct ccatgagctg tctcataaat
     9061 gactttatgt gattttcatt aaaatgaacc aagcctgatt ccagtagtcc catcagatca
     9121 tggtccatat attcaaacac cagataaaat gcacctttgt ccttcttgaa atccaaagca
     9181 tcttctttat cagtcactat ttccttcata ttgataatac tctgatgggt aagctgccgg
     9241 agaattttaa tttctcgaat tgctgtaatt ggaaagcctt ccttttcatt atccagacgt
     9301 acttttttta aggctaccat ttctccagtg tctttatccc tggctttgta aacttgtccg
     9361 taagtacctt ctccaataat tccgatgata tcaaatttat ccacgcagcg ttttccccag
     9421 tcaatatctt tttctttggt ttcaccatag cgaggcccac atattttagg cctcctttta
     9481 ctatgtaact gtgttgctgt tttcttttcc tctggactct ttgaaagatc atctcctcct
     9541 ggtagctcag ggggcagcgg taaatcagca agaagacatc ggagtttctt ttctacttct
     9601 tttttaactg cttttactga aatatttcct cgtaagctat cagcttcttt atcttcaggc
     9661 agcatgggag gcaaaggtaa cggtggtaat gtagaggtga ctaaagctac atgttgctcc
     9721 ttctccttgg ctcctatgct tggtgtaagt ggtttggttt tctctttaag agatactgat
     9781 tcctcctttg tagcagcaga tttactctcc tttccaacta tgactgcttt cttggtggct
     9841 ttatctacaa tcaaattatt ttccaccttc gttacctgaa gaggtggctt tgtttttgct
     9901 ttgtcatttt ttaaagttcc accacttgag ggagaaggtg catgttcaat tttaattttc
     9961 ttcacatcct tcacatggtt tgtttgtgat gcactggcac tagtttccgt gttccccttg
    10021 gtaggtgtag aagtgtttga agcttttgca gccttggcag cagcctcagc agccttagtt
    10081 gcttctgcag ctttcgctgc ttctgcggct ctcgctgcct ctgctgctcg tgctttttta
    10141 ttcttgttca attcagctgc caggctactc ttcagagtta gtgtgctagg agaaatacta
    10201 gaatgacgac ttctggatct agacaatctg tgcctgctac gagatcttga atgcctagat
    10261 gaatacgggc ttctgcttcg ggattttcca gaccgtctga gcacagggct gtagggactg
    10321 cgagagcggc gccagctgct gctgctgtag ggactagggg acacgtcgcc gccccgctcg
    10381 taggagctgt ggcggctgta gctgggggag cggcggcgac tgtaggggct cggggagcgc
    10441 ggcagccgcc gagaataggg gctgctgcca cctcctgccg ggctggggga cttgcggctc
    10501 ctcaggctct gagaggccct gtgggacacc gggctgtcgt cccggcctcc cagtgggctg
    10561 agggaccgcc gccgcctgta ggccttaggc tcggtcttgt cctcccggta ggccttgggc
    10621 ggttccttgt aggccgaagg cggctccttg gacgacttag tccggctgcg gtgggccttc
    10681 gagtcccggt ccttgcggct gctactgctg ctggatgtgg ccgaagcgct tttccggcgg
    10741 ccgccgctgc tgctgctgct gccgctcttg gcgacctcgg cccgttcctc gccgctgtgg
    10801 ctgtggcggc tgcgggactt ggaggcctcg ctgccaccgc gctgcccatc ccgccgccgg
    10861 tgctcgcggt ggcgctcctt gctgcggcca ctgctgctgc ggcggtcccg gcggggcctg
    10921 cgctcgcttc cctctccacg gcgctgtgta ccggagcttg aggcgggtga gcctccacta
    10981 cctcctgtgc cgcctgcagc tgtggctgcg gttgcggctg aagcgccacc caacagcaag
    11041 ccttgctcag attggctcga cacgtcttcg tactcgacca atggggtgac acctccgcct
    11101 gagctagcgc cacctccgcc gtcctgttgc ggctgaggca gcgagaaaac acggcgcttt
    11161 tcagcctctt gtccagctct tggaccacgt ctgcgctttt gacggcctcc tgccctcctt
    11221 tttcctctgg ccaaacgttt cacctccagt ggaggaccgg gggagaagca gctggaagag
    11281 gctgcggcag cagctgcggc cgcggccgct gtacctggtg cagccaggaa caagagaggc
    11341 ggtggagggg gagggggctg caacagttga ggctgcaaca gagggagcaa caagggtggc
    11401 tgttgaggcg acaagaagcg cctacgcttg cgtctctctt cgagtttctt ctcggcccat
    11461 gacaatcctc caccgccacc gagggccgtg tctgacgaac ttgggggtcc ctgaaagagg
    11521 acttcaagat cgagcttgtc atcgtcgtcc ttgtagtcca tggatccgcg cccgatggtg
    11581 ggacggtatg aataatccgg aatatttata ggttttttta ttacaaaact gttacgaaaa
    11641 cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc atgatctatt
    11701 aatattccgg agtaggtcga tttaaatctt tagaccatag agcgttcact tgacagagtg
    11761 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat
    11821 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
    11881 cagttagcct cgataaaaac taagctatgt gaaccgttta aacccagata acttcgtata
    11941 atgtatgcta tacgaagtta tggtaccgcg gccgcgtaga ggatctgttg atcagcagtt
    12001 caacctgttg atagtacaaa caaagctgta gccaaccact agaactatag ctagagtcct
    12061 gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg
    12121 tcagcaccac cggcaagcgc cgcgacggcc gaggtctacc gatctcctga agcaagggca
    12181 gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg
    12241 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
    12301 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacaa acccagttga
    12361 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
    12421 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
    12481 atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
    12541 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa cgatgttacg
    12601 cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat cattcgcaca
    12661 tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt
    12721 gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta cctcgggaac
    12781 ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc ggttgttggc
    12841 gctctcgcgg cttacgttct gcccaagttt gagcagccgc gtagtgagat ctatatctat
    12901 gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct catcaatctc
    12961 ctcaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc agattacggt
    13021 gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt gatgcacttt
    13081 gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg cttcccggcc
    13141 gcggagttgt tcgg
//
