LOCUS       sequence-461165-       16195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Lopez Martinez D, Todorovski I, Noe Gonzalez M, Rusimbi C, Blears D,
            Khallou N, Han Z, Dirac-Svejstrup AB, Svejstrup JQ
  TITLE     PAF1C-mediated activation of CDK12/13 kinase activity is critical 
            for CTD phosphorylation and transcript elongation.
  JOURNAL   Mol Cell. 2025 May 15;85(10):1952-1967.e8. doi: 
            10.1016/j.molcel.2025.04.012. Epub 2025 May 1.
  PUBMED    40315851
REFERENCE   2  (bases 1 to 16195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.04.012"; journalName: "Mol Cell"; date: 
            "2025-05-15- 15"; volume: "85"; issue: "10"; pages: "1952-1967.e8"
COMMENT     Sequence Label: pBIG1a-PAF1CdeltaCDC73
FEATURES             Location/Qualifiers
     source          1..16195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1082..1247
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    1562..1696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2786..2877)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3033..3167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4935..5026)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5182..5316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7489..7580)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7736..7870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8003..8026)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(8027..8050)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(11618..11709)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    11804..11852
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     11877..11896
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    11942..11975
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(12371..12376,12377..12393,12394..12399)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             12588..13121
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  13188..13412
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(13546..13563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13717..14305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13797..13816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14476..15267,15268..15336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15099..15118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15337..15441)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15467..15921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15604..15625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15815..15834
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(15962..16195,1..558))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
       61 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      121 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      181 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
      241 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      301 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      361 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      421 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      481 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      541 gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac
      601 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
      661 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
      721 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
      781 ctacttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga
      841 ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat catttattct
      901 gcctcccagt tcaggctgca aataagcgtt gatattcagt caattacaaa cattaataac
      961 gaagagatga cagaaaaatt ttcattctgt gacagagaaa aagtagccga agatgacggt
     1021 ttgtcacatg gagttggcag gatgtttgat taaaaacata acaggaagaa aaatgccccg
     1081 ctgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta
     1141 tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa
     1201 attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcgg atctcctgtt
     1261 taaaccccga ttgagatata gtttttaatt aacccgtagt ggctatggca gggcttgccg
     1321 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tgggggttgg ggtggggaaa
     1381 aggaagaaac gcgggcgtat tggtcccaat ggggtctcgg tggggtatcg acgtaacccc
     1441 gattgagata tagatttatt taaatggtta tgatagttat tgctcagcgg tggcagcagc
     1501 caactcagct tcctttcggg ctttgttagc agccggatct tctaggctca agcagtgatc
     1561 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1621 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1681 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1741 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     1801 tcctctagta cttctcgaca agcttttaaa ttggacaatc atagatgtga atttcctggt
     1861 catctccaac agacacaatt tttgaaccat ttccattgta ttttactccc cagacctgat
     1921 cctggtgatc aaagaaggtg tgaacacaag tcctcgttcc aacatcccaa acttttacac
     1981 ttttgtcaga cgaactggaa acaaagtgag tgtcatcagg acagaatgca acgttcagca
     2041 cccaggaggc atggccgctc agcgtgccag ccaaattggc atgttgtaca tcatagatct
     2101 tgatgtagcc atcatctgaa gcagtgacaa ggagctggga gtccggggaa aaggtcaagg
     2161 agcgaatggg catggcatgg ccttccaggg tatgcagaag ttttccagtt gcaatatcaa
     2221 aaatattgat gattccatct atggctccac tggctaggta tttcccatca ggactatatg
     2281 caatactaag aatgaatttt cctctcgtgt ccaaagaata ttcctttttc ccactttcca
     2341 caccaaaaat gttcactttc ccgacatgag ttcctgtggc cagatactgg gaatcaggag
     2401 aaaaggccaa agtccaggca tccacaggtc ctgcatctat ggactttatc tgtttgccat
     2461 tttccaagtc ccaaagacga atatgagcat caagagagct ggatgcagca atgggcaggg
     2521 tgtggctgat gtccacagac accactccca gctgatgtcc ctccagactc cactgtaggt
     2581 ccagcctctc atcacgccat ttccagacct tcaccaggtc atctagggag cctgtgacca
     2641 ctgtctcaga gttttccttc ttgtttgtcc cccaagcaac tgaccaaatg gcatcatcat
     2701 gggcttgctc ttgtttgaag agaataccgt actggttggt catggatccg cgcccgatgg
     2761 tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa
     2821 aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta
     2881 ttaatattcc ggagtaggtc gatttaaact aagctatgtg aaccgtttat ttaaatggtt
     2941 atgatagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
     3001 cagccggatc ttctaggctc aagcagtgat cagatccaga catgataaga tacattgatg
     3061 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3121 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3181 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3241 acctctacaa atgtggtatg gctgattatg atcctctagt acttctcgac aagctttcag
     3301 tcactgtcac tatcagcttc actggaatca gaagctgcag cttcactgcc atcctcctgg
     3361 gccgagtgct cgctgccact ggggaaggga ctggcgctgc ggctgtggct ccggctccgc
     3421 tggccacccc cattgctgcc gctgtctgaa tcattgtcac tgccaccttg ggcctgtcct
     3481 ctgtcctcat catcagagtc ggcatcgtcc tcagaatcag catcactgcc aaagatctcc
     3541 tctttgtcac gggcagcccg ggcctcatcc tcgctgctct cgtcctcacc actgccactc
     3601 ttgtcactgg cctcgtccct gtcaccttcc tcccgttcac tctcgctgcc cgagtgctca
     3661 tcttcactgc cctccttctc actgctgctg cccttctcct gctcctcatc tgagccccca
     3721 gcttctttct cttctgtctc catctcctct tcctcttcct cctccggttc gtggttttct
     3781 agctgggcct tccgtgcctc ctgagcttcc agttccttct cattcatgtc ccgatgtttg
     3841 accacaagca gggcgttggt gcctgactga accccagcct tggcccggcg cttactaagg
     3901 cggaccctgg tttccaactc attgtagtaa accccgtcac cctctcggaa gatgaagaag
     3961 tagttttcct catagccctt gctagctttg ttcttcacgt tccagttgta ctcccgagca
     4021 attttgtagt catacacatc atctggtgca tagtccatct cctcctcctg gtcccgcttt
     4081 cgtttcttca acgtctcttc tacaggcagg aaataggcca caaactggtt cccttcctca
     4141 tccatcatgc ccctaatcat ggcctgagac atcatctcca acgcagctgc accactcgtg
     4201 tccttggggg ctgggtctga gtcaaagatc acctgagcac atggattgat ccacatctta
     4261 aagtctggga agacaggcat gacctccacc ggtgtgactc ggggtttgct gtaatgctgt
     4321 gagattgatt tctgggcatc ctcaaaagtc ttctcaatgg ctgtgatctg gctatccctg
     4381 tctttgtata tttcttcctc ggtaaactgc tgcttcacag aaaccccaat cttgacctca
     4441 ggcttctcat tggagatgcc ataacggttg aactcagtgg agatgtactc tgtctttcgc
     4501 atccatggca ccaccttcgc gtgctgctgg gatctcttgg agctggtggg ggcctgaatc
     4561 tcctcttcca aaagtttctc atcagctgga tctagaagaa cattggggtc gatgcggtag
     4621 gtgtcaggat tgatgagatc gatggtgacc cccaggtctg gctcagtcag gaggtcatgt
     4681 ttgtgctgtt tctccaagga agtggctttg tactggacga acctgttctg gtcgaagggg
     4741 taggtgatga acttggggtc gaaggggata tcagggaggc tattgcagta cttgactcgg
     4801 cagaccactc cagacctctc aggcagagtc cggtgggaat tgggcctgtg gccatcctcc
     4861 cgctgggcct gggtctggat ggtgggcgcc atggatccgc gcccgatggt gggacggtat
     4921 gaataatccg gaatatttat aggttttttt attacaaaac tgttacgaaa acagtaaaat
     4981 acttatttat ttgcgagatg gttatcattt taattatctc catgatctat taatattccg
     5041 gagtaggtcg atttaaacat caggcatcat taggtttatt taaatggtta tgatagttat
     5101 tgctcagcgg tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatct
     5161 tctaggctca agcagtgatc agatccagac atgataagat acattgatga gtttggacaa
     5221 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5281 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5341 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     5401 tgtggtatgg ctgattatga tcctctagta cttctcgaca agctttcaat catcatcttc
     5461 ttcctcttca tcgctgatca catacttctt atgcttttta tttgctttat catcatcttc
     5521 tgcttttctc tttccggaag gttcaccttc ctcatcactg gtaagtttct ttgctttgag
     5581 taatctttga gctttatctt cttctgatcc ctcatcactg tctgatgaat agattctggc
     5641 tcgttcctct cgaatgcccc ctttatatcg gtttttaatg gcagccaagc tgatggactc
     5701 ctcgccttcc tcctcctcat cgtatcgatc aggttccagg taactggcgc tcagcccccg
     5761 ctggtgctgt ttctctctca ttcggcgctg ctgagattcc ctacgtatgg aagccctcaa
     5821 acgttcttct tctttcttaa tcatttctgt gcgttggcat tcaggatcac gaccagccat
     5881 tggcaagatt ctaatcttct gtgtctttga acacctatct gcaagtgaca gagtcatctt
     5941 tctatgtgtg gcactgtccg tagagtgagg tctgaaggtg agtttcgttt taaagactgc
     6001 ttgtccctgt agaccagtac cttgtcttat aaaaagatga ttgtggtcgc cctgcagtgg
     6061 ggctttgtac acatcaaaca cttcattgcc taaatgcagg gacatgcttc catctgacca
     6121 cttgactatc cgagcattgc tttctttaat ttcatttcct tcttcatctc ggcgtatcct
     6181 ccatcttata gtattttcta cctttaattt taacctggtt ctaccttctt catccagcat
     6241 ttcttcatct tcaaattcat cttcataata ctgaggatca aaaggtctgg gctctacact
     6301 gagaaagttg ggcagtttaa caaaatataa gtcgtttcct aaatcagtgt ttactttggg
     6361 tatttctact tctattctgg tctcaggaat tggctcctct tcctgttgat cctgaggcaa
     6421 tccattttca tcaacaggct gtcctggagt aggtggtttg tcttctccat cactccctga
     6481 agagatatca tctgcacctc caaataaatc catggttcca ctattatctt ttggcacctc
     6541 agtgtcacta tccgcttctg aatcagatgc aatcgcattc ttgcgtttca ttcgtaaaac
     6601 ttcatcttca ctatcactgc ctcttgcaga ttctgattta tgatcctgtt cctcttcatc
     6661 atctgaatgc ctgtgttctt catctgaggc ctgtggcctt tcatcatcag aattttgcat
     6721 tttctcttca tcagacagct gtggttgttc ttcatcatca gaattctgtt tctcatcatc
     6781 attatcagaa gctaccggcc gttcatcatc agaattagcc ttttcctcct cagatagctg
     6841 ttgtctctca tcatcggaaa gctgaggcct ctcctcatca tctgtgttct gcattttctc
     6901 atcatcgtca gaattctgca gcttatcttc atcagatcct tgtgccctct cctcatcatc
     6961 agaattttgt atcttttcat catctgactg gtcactttta tcttctctgc cccatttttc
     7021 atcatctgaa tgtgcttttt cagaaccttc tgcttctgaa tgatggctcc ctccatccga
     7081 tctatgacct tcgtcttcat catcattagg ggcttctgat ccactgtgct gatctacatc
     7141 tgaggggtca ttgtcctcat ggtcagaacg ctcagaagct tctgatctat tgtctgatct
     7201 ttcagagtga ttatcactac cactatgatg tgaagctccc tcgtcctcac tgtcatctcc
     7261 aaacagttcc ttattacttg gttgtcctga atcacctctt tcatcctgat cactttcact
     7321 tccagaggca ttactgccag aggcagcatt ctcttgatca gaatctgagt cagatccaga
     7381 atcagaatct ttacgctcag cttcgctgtc ggcgtcgctc ccgaagagat cctccatatc
     7441 cgccatggat ccgcgcccga tggtgggacg gtatgaataa tccggaatat ttataggttt
     7501 ttttattaca aaactgttac gaaaacagta aaatacttat ttatttgcga gatggttatc
     7561 attttaatta tctccatgat ctattaatat tccggagtag gtcgatttaa acgtgcaata
     7621 gtatccagtt tatttaaatg gttatgatag ttattgctca gcggtggcag cagccaactc
     7681 agcttccttt cgggctttgt tagcagccgg atcttctagg ctcaagcagt gatcagatcc
     7741 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7801 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7861 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7921 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     7981 agtacttctc gacaagcttt cacttttcga actgcgggtg gctccagggt ccctgaaaga
     8041 ggacttcaag tgcaccgtca ctatcatctg acccatgttc tgagcctcta tctgaggcct
     8101 cattgttgga tccctctggt tccgattcat ttccagagcc ttggccagaa ccctcattat
     8161 cagatcctct ctccgattcg tgatctgatt cggcacttgg agaagctggg cgagagtcgt
     8221 tctcagaaac tcctgagtgg cttctccctg actgcacaga ttcattgtca gactgctcag
     8281 aaccggaggg ccttctcttt ctggatggct ggtcactgtc tgagtcctga tctgaccgct
     8341 gtcttcgtgg cctccgggga ctgccggcct cgctgccaga cttgttctgg ttctcatcgg
     8401 aatcactctc tgatgaggca catttctttc gttgttcgtc ctcgtctgag tcactgttgc
     8461 tgttgctgtt cctgggatgt ccttcatcag caattttaag tttatcctca tccgaagagt
     8521 catcacttga tgaaattatg gctttggatt ttatttttcc cttcattgat ggaggcagac
     8581 gttctggctt gggagccttt ttcttctctg cttttggtgg acgtcgtttt tttggtttgg
     8641 ggccattttc tgtttcatca tcatcagatc cttcttctcc ctttggatgt cttctcctct
     8701 ttttcttctt tctctcacca ccctcctcat cttcaccttc ttgttcactg ccactaccct
     8761 ttcttctctt cttctttttg gatataggta ggtcatcatc agtgtcatca ttgacaaatt
     8821 catcaaactc tcctcccttc ttagaacgcc gtccaccacc accacctctt ttcttctctt
     8881 ttgttgcttc agtctcacca gtaaacataa gaatattttt ggtcttctcc acatactggg
     8941 cccgctgttc caaaagtttc ttttgctctt ccttttctct gagacgtttc tcttcctgtt
     9001 ctttaagaag tttctgcctt aacagctcct tttcttgctc ttgcttggcc cgcagctccc
     9061 gctcttcttc atcttgtttg cgtgcccggg ccacatggta ctgggcctgg ctcagtaagt
     9121 cagaacactg cctggcttct gtagcagcaa gggccaaatc aaatctcatt ttatctccca
     9181 ctttactcaa ataactgaag tatctatgtg caagctccag ttctttcaca gcattaagta
     9241 cttccttcag attacttttt tcatctttca ggacagaggt agctaatctt tgcaggacca
     9301 aggccacatt aaacataaga actgtatcac tgggtgccac atgtctagcc ttcagcaaag
     9361 tctgtttgca ttcctgtaac ttgccacact tgaagagggc ccgggccaaa tagagtacaa
     9421 cttcagtgtt ttggtgctta tagaactttc ggaggcagtt ttcatacatc tgaacggcgc
     9481 tgatgtactg cttttgctcc acatagatgt gtgctaagtt cagccacaca tcactaatat
     9541 ctgctgttgc ttctcttact tgggcaaata catcacgagc ttcacgaaaa tatcctttgt
     9601 gggccaaaac agctcctatg ccattggcag catacagatt ctttgcatca tttctgagta
     9661 cttgtttgta gatggccaga gcacgatctt gatgacgctt ttccttttct cgatctcggg
     9721 tgggctgatg taaagtttgg agccacacgt tgccaagggc tagcatagaa taggtatcac
     9781 tctgtgtgga tggctgtttt aatatcctct caaacttctt ctgcccagga ccccattctt
     9841 gttttgccaa atgaagattg ccaatcaaag accaagcatc tggatgatcc tgattaatct
     9901 gaagagcttc cttaaaccaa tctgaagcct cataaaagtt tcccttatct ctagccatgg
     9961 ctcctaggcg caaatagcag tcaacataat taggatgttc gcgtaagatg tttttataca
    10021 gtttttctgc ttcatggaat tcacacatcg cctcatatag cctggcgaga ttatatgacg
    10081 tagtaacgga aatggcgtta tagtaatgct catcgtgttc cgcttctgct tttgcacggt
    10141 ccaatgacgc caaaaaatat ttcttagcct cccctaggtt tccaagtcta aaatggaggg
    10201 cacccacatt attgagaatc tctggaggaa catcggcctg cactttctcc tgaaggattc
    10261 gtgttgctgt tccataggct gaaagggcac cctgtatatc agtctgttct aagatttgtg
    10321 ccaattcaat ccaagcttca acatcatcgg gatactgttc tgtgaccttc ttcaaatggc
    10381 ccttggcaat atctcgtttt tcttgatctt ctgaggcagc atagagagag ccgagaattt
    10441 tcatagtttc gtaattatta ggataagctt tcaaaacctt ctcaaagcac tgagatgcat
    10501 tttctttgtc acctcgataa atatacattt gtcccaaacc aaaaaatggg agcacaaaag
    10561 aggatgaggc aaactgtgtg gcttgataat agtactgaaa agcttggtca taatcttcct
    10621 gaacatggaa tgatctagct agctgatagc agctctctgc ttgcatagct tccacttctg
    10681 tattatggaa tgcatggagg gccagatgct ggactttact ataatccttt ttgaagaaaa
    10741 agtgatttgc caaatggttc aataccatag ggttgctagg atcaatagta taggctctgg
    10801 aaagaagctg gacaccattt ttaatggaat cagcctcttt attgttgagt tctagaacag
    10861 ccagtccaac caatgctccc acgcatttgg aattgagttc cagggctctg ctgaatgcca
    10921 gacgagcttt ttccagtttg ttaagtttca caaagcaatg acccattcct aaacgaactt
    10981 ccgctggaca tcctgggtta gtacgcaatg ctttcttata gtaagcaaga gctcctctgt
    11041 aatccttctt gttgaaggaa atgcaagctt taccaagaag ggctggaata ttatttggag
    11101 actgattgag tacaaaatga aactgtgcat cagcttgatc cattttgtca ccctcaagta
    11161 ggcagaagca ggctcttccc aacaaatggt tctgatcata cataataatt ttatcggcca
    11221 ttgtatacaa caaggtggcc tgtgtaataa gatccttttt attgtcctta ttcttttctt
    11281 tccgagcctg ttgtacataa tacgctgcca atgtatccaa gcaagtcatc tggtcttttt
    11341 catggtctct atagtccaaa ttgccatcta tacgtgctgc ttccaacaat tttacaaact
    11401 cttctgtttt tccttgcttg tagtattcca gcgccaaagc aatccatatg tgcagttgtg
    11461 tgtgttcctg tttcagaata ctgataactt catctccctc cggtaactga tcgaagtcaa
    11521 gttcaatgac ctcgtcagtg tcccggaggg gaatctcgat ggagccccgc gacatggatc
    11581 cgcgcccgat ggtgggacgg tatgaataat ccggaatatt tataggtttt tttattacaa
    11641 aactgttacg aaaacagtaa aatacttatt tatttgcgag atggttatca ttttaattat
    11701 ctccatgatc tattaatatt ccggagtagg tcgatttaaa tctttagacc atagagcgtt
    11761 cacttgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
    11821 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
    11881 ctccttccgt gtttcagtta gcctcgataa aaactttaga ccatagagcg tttaaaccca
    11941 gataacttcg tataatgtat gctatacgaa gttatggtac cgcggccgcg tagaggatct
    12001 gttgatcagc agttcaacct gttgatagta caaacaaagc tgtagccaac cactagaact
    12061 atagctagag tcctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac
    12121 cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc taccgatctc
    12181 ctgaagcaag ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa
    12241 tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc
    12301 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
    12361 acaaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
    12421 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
    12481 tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca
    12541 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
    12601 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg
    12661 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg
    12721 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg
    12781 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag
    12841 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg
    12901 agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg
    12961 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc
    13021 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag
    13081 aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga
    13141 tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccgcgaa tatagtcttt
    13201 accatgccct tggccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga
    13261 agagtttgct ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga
    13321 tttcagttta caactattct gtctagttta agactttatt gtcatagttt agatctattt
    13381 tgttcagttt aagactttat tgtccgccca cacccgctta cgcagggcat ccatttatta
    13441 ctcaaccgta accgattttg ccaggttacg cggctggtct atgcggtgtg aaataccgca
    13501 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
    13561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    13621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    13681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    13741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    13801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    13861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    13921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    13981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    14041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    14101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaat
    14161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    14221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    14281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    14341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    14401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    14461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    14521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    14581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    14641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    14701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    14761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    14821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    14881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    14941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    15001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    15061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    15121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    15181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    15241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    15301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    15361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    15421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
    15481 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
    15541 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
    15601 gttccagttt ggaacaagag tccactatta agaacgtgga ctccaacgtc aaagggcgaa
    15661 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
    15721 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
    15781 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
    15841 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
    15901 atgcgccgct acagggcgcg tcccattcgc caatccgtcg gcttgaacga attgttagac
    15961 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
    16021 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
    16081 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
    16141 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcac
//
