LOCUS       sequence-461171-       11802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human INTS4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Sood V, Holewinski R, Andresson T, Larson DR, Misteli T
  TITLE     Identification of molecular determinants of gene-specific bursting 
            patterns by high-throughput imaging screens.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):913-928.e8. doi: 
            10.1016/j.molcel.2025.01.022. Epub 2025 Feb 19.
  PUBMED    39978338
REFERENCE   2  (bases 1 to 11802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.022"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "913-928.e8"
COMMENT     Sequence Label: pLV EF1alpha INTS4 IRES Cherry
FEATURES             Location/Qualifiers
     source          1..11802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3559..3568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6473..7045
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6496..7047
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6640..6657)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             7047..7757
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7192..7210)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7460..7479
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7771..8359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7824..7844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8242..8253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8566..9199
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9327..9343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9327..9343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9340..9362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9351..9367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9375..9381,9382..9399,9400..9405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9420..9441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9558..9575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9729..10317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9809..9828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10488..11279,11280..11348))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11111..11130
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11349..11453)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11501..11635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11538..11557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11592..11611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gttctagagc caccatggcg gcgcacctta agaagcgggt ttatgaggaa
     3601 ttcacgaaag tggttcagcc acaggaggaa attgctacta agaaactccg actaacaaaa
     3661 ccaagtaaat ctgcagcact ccacatagat ctgtgtaaag ctacctcccc agcagatgct
     3721 ttgcaatact tgctccagtt tgccaggaag cctgtcgagg cggaaagcgt agagggagta
     3781 gtcaggattc tcttggaaca ttattacaag gagaatgatc catctgtgag actgaaaatt
     3841 gcatcattgt tgggtttatt atcaaagaca gcaggatttt caccagactg cattatggat
     3901 gatgccatca acatcctgca gaatgaaaag tctcatcaag tcctagctca actgctggat
     3961 actttgcttg caattggcac taagctacca gagaatcaag ctatccaaat gcgattagtt
     4021 gatgtggcct gcaagcatct gacagatacg tctcatggtg taagaaataa gtgcctgcag
     4081 ttacttggca atcttggctc tttggagaaa agtgtcacaa aagatgcaga aggcctagct
     4141 gccagagatg tccagaagat tataggggat tacttcagtg accaagaccc acgtgtcaga
     4201 acagcagcta taaaagccat gttgcagctc catgaaagag gactgaaatt acaccaaaca
     4261 atttataatc aggcctgtaa attactctct gatgactatg aacaagtgcg cagtgctgca
     4321 gtccagctta tctgggtcgt cagtcagctc tatcctgaaa gcattgtccc aattccttct
     4381 tctaatgaag aaatacgctt agttgatgat gcgtttggca aaatttgtca catggtcagt
     4441 gatggctctt gggtggttcg tgttcaggca gcaaaactgt tgggctctat ggagcaagtc
     4501 agttctcatt tcttggagca gacccttgac aagaagctga tgtcagatct gaggaggaaa
     4561 cgtactgcac atgagcgtgc caaggaactt tacagttcgg gggagttttc cagtggcaga
     4621 aagtggggag atgatgctcc caaggaagaa gtagataccg gggctgtgaa cttgattgag
     4681 tcaggagctt gtggagcttt tgttcatggg ttggaagatg agatgtatga ggttcgtatt
     4741 gctgctgtgg aggccctctg catgttggcc cagtcttcac cctcttttgc tgagaagtgc
     4801 cttgatttcc tagttgacat gttcaacgat gaaattgagg aagtacgtct gcagtctata
     4861 cataccatga gaaaaatctc taacaacatc accctccgag aagatcagct tgacactgtc
     4921 ctggctgtgc tagaggattc atccagagat attcgagagg ctcttcatga actcttatgc
     4981 tgtactaatg tttcaaccaa agaagggatt catcttgcat tggtggagct gctgaaaaat
     5041 ttaaccaagt accctactga tagggactcc atatggaagt gcttgaagtt tctgggaagt
     5101 cggcatccaa ccctggtgct tcccttggtg ccagagcttc tgagcaccca cccatttttt
     5161 gacacagctg aaccagacat ggatgatcca gcttatattg cagttttggt acttattttc
     5221 aatgctgcta aaacctgtcc aacaatgcca gcattgttct cagatcacac cttcaggcac
     5281 tatgcctacc tccgagacag tctttctcat cttgttcctg ccttgaggtt accaggtaga
     5341 aaactggtgt catcagctgt ttctcccagc atcatacctc aagaggatcc ttcccagcag
     5401 ttcctgcagc agagccttga aagagtgtat agtcttcagc acttggaccc tcagggagcc
     5461 caggagctgc tggaattcac catcagggat ctgcaaagac ttggagaact tcaatctgaa
     5521 ttggcaggag tagctgattt ctctgccacc tatcttcgct gtcaactact tctcatcaag
     5581 gccttgcagg aaaagttgtg gaatgtagct gcccctttgt atttgaagca gagtgatttg
     5641 gcctcagcag cagcgaaaca gattatggaa gagacctaca aaatggaatt catgtacagt
     5701 ggtgtggaga ataagcaggt ggtgattata catcacatga ggctgcaggc caaagctttg
     5761 caacttatag taacagcacg aactacacga ggacttgacc ccttatttgg gatgtgtgaa
     5821 aaatttttac aggaagtaga cttttttcag aggtatttca tcgctgattt gccccacttg
     5881 caggacagct ttgtggacaa actccttgac cttatgcccc gactcatgac atccaaacct
     5941 gcagaagtgg tcaaaattct acagaccatg ctgcgacaga gtgcctttct gcatctcccg
     6001 cttccagagc agatccacaa agcctcagcc accatcatcg agccagcggg cgagtcagac
     6061 aaccctttgc ggtttacctc tgggttggtg gttgccctgg atgttgatgc aaccctggag
     6121 catgtgcagg atcctcagaa cactgttaag gtccaggtct tatatccaga tggccaggct
     6181 cagatgattc accccaagcc tgcagacttc cggaatcctg gcccagggcg gcaccggctc
     6241 atcactcagg tttatctctc ccacaccgct tggacagagg catgccaggt ggaagtgagg
     6301 ctgctgctgg cctacaactc cagtgctcgc attccaaaat gcccctggat ggagggtggt
     6361 gagatgtcac cacaggtgga aaccagcatc gagggcacca ttcccttcag caagcctgta
     6421 aaagtttata taatgcccaa acctgcacgg cgctaagcgg ccgcggatcc cgcccctctc
     6481 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6541 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6601 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6661 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6721 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6781 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6841 gttggatagt tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac aaggggctga
     6901 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6961 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     7021 tttcctttga aaaacacgat gataatatgg tgagcaaggg cgaggaggat aacatggcca
     7081 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     7141 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7201 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7261 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7321 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7381 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7441 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     7501 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7561 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     7621 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     7681 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     7741 acgagctgta caagtgaacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     7801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8161 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8341 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     8401 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     8461 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     8521 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     8581 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     8641 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     8701 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     8761 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     8821 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     8881 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     8941 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     9001 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     9241 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
     9301 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9901 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    10081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    10141 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    10201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    11041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11461 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    11521 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11581 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11641 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11701 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    11761 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
