LOCUS       sequence-461185-        5826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer near RNF157, chr17:76166910-76167743 cloned into pGL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  TITLE     Wysocka Lab enhancer plasmids 2025
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3_RNF157
FEATURES             Location/Qualifiers
     source          1..5826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1014..1135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1105..1124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1176..2828)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2744..2762
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      2825..2834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2864..3060)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2878..3013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    3925..4016
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3957..3976)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    4030..4078
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4209..4664)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4346..4367)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4558..4577
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4691..4795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4796..4864,4865..5656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5014..5033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctgccg
      781 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
      841 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      901 ggatccagat ccttatcgat tttaccacat ttgtagaggt tttacttgct ttaaaaaacc
      961 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1141 tctgctcgaa gcggccggcc gccccgactc tagaattaca cggcgatctt tccgcccttc
     1201 ttggccttta tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc
     1261 ggtacttcgt ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg
     1321 gcgacgtaat ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg
     1381 gcggcgggaa gttcaccggc gtcatcgtcg ggaagacctg cgacacctgc gtcgaagatg
     1441 ttggggtgtt ggagcaagat ggattccaat tcagcgggag ccacctgata gcctttgtac
     1501 ttaatcagag acttcaggcg gtcaacgatg aagaagtgtt cgtcttcgtc ccagtaagct
     1561 atgtctccag aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg
     1621 tttacataac cggacataat cataggacct ctcacacaca gttcgcctct ttgattaacg
     1681 cccagcgttt tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta
     1741 ccgaccgcgc ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca
     1801 gtgagcccat atccttgcct gatacctggc agatggaacc tcttggcaac cgcttccccg
     1861 acttccttag agaggggagc gccaccagaa gcaatttcgt gtaaattaga taaatcgtat
     1921 ttgtcaatca gagtgctttt ggcgaagaag gagaataggg ttggcaccag cagcgcactt
     1981 tgaatcttgt aatcctgaag gctcctcaga aacagctctt cttcaaatct atacattaag
     2041 acgactcgaa atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg
     2101 aatggaacaa cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct
     2161 ctggcatgcg agaatctcac gcaggcagtt ctatgaggca gagcgacacc tttaggcaga
     2221 ccagtagatc cagaggagtt catgatcagt gcaattgtct tgtccctatc gaaggactct
     2281 ggcacaaaat cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc
     2341 gactgaaatc cctggtaatc cgttttagaa tccatgataa taattttttg gatgattggg
     2401 agcttttttt gcacgttcaa aattttttgc aacccctttt tggaaacgaa caccacggta
     2461 ggctgcgaaa tgcccatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc
     2521 gcaactgcaa ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt
     2581 tcactgcata cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc
     2641 aaccgaacgg acatttcgaa gtactcagcg taagtgatgt ccacctcgat atgtgcatct
     2701 gtaaaagcaa ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct
     2761 ccagcggttc catcttccag cggatagaat ggcgccgggc ctttctttat gtttttggcg
     2821 tcttccatgg tggctttacc aacagtaccg gaatgccaag ctttttgcaa aagcctaggc
     2881 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     2941 ctgcataaat aaaaaaaatt agtcagcgat ggggcggaga atgggcggaa ctgggcggag
     3001 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     3061 gatcgcagat ctaagatctg acacagatgc tcgggtagcc atcaccatct attaccaggc
     3121 cacggaagag ttccagaatg gtattgccag gtaagatcag gagaccagga gagccacttc
     3181 cttcccagat tattaccagg ccacggaaga gttccagaat ggtattacca ggtaagatca
     3241 ggagaccagg cgagccactt ccttcccaga tagggagagt ccatgtctta tccactcagc
     3301 aagggcggaa aacattcttt ttctgcagcc agatgagatc tggttcctga atttagactt
     3361 tcttctacaa agccctaccc tttacctttt cccttcaaat tgtgccatct tggtatagtg
     3421 acaaatggtg gtatagtcct tcaggtcctt aacatgctgt taaaaatcta gtaagctacc
     3481 tttctttgga tgtgtgggtt gaatagaaaa gtttaacgtt gccaagcaag agcaaaggaa
     3541 atggattata aaggaccctt tcttctcttc gctagaccct gttgataatt gagaacaggc
     3601 atgagcagag gaagttgtca gacccatctg tgccggatac tgacttttgc tttgcctccc
     3661 acttgtatca atagctacat tcccaaagac aacagcctcc agtcggagac tgtgcagtac
     3721 aagcgaggag tgtgtcagca gttctgcctg ccctcccaca ccgtggatcc ctccgagtgg
     3781 gccgaagagg aggtgagctg cctctgggga gggtttccca catccactca gaagggccta
     3841 ggggctactt aggactcggt ctgacagcaa aggggctgct cggatgcttg agctagcacg
     3901 cgtaagagct cggtacctat cgatagagaa atgttctggc acctgcactt gcactgggga
     3961 cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta
     4021 ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc
     4081 ggccgctcca agtacctccc gtaccttaat attacttact tatcatggta gcttgggctg
     4141 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4201 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4261 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4321 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4381 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4441 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4501 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4561 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4621 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     4681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5821 atcttc
//
