LOCUS       sequence-461190-        5923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer near C8orf76, chr8:123232060-123232824 cloned into pGL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5923)
  TITLE     Wysocka Lab enhancer plasmids 2025
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3_C8orf76
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(77..96)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(756..1547,1548..1616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1379..1398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1617..1721)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1748..2203
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1835..1854)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2045..2066
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2334..2382
                     /note="synthetic polyadenylation signal"
     misc_feature    2396..2487
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2436..2455
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3449..3645
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3496..3631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      3675..3684
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3681..5333
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3747..3765)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5374..5495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5385..5404)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5439..5458
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5749..5766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5920..5923,1..585))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
       61 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      121 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      181 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      241 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      301 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      361 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      421 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      481 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      541 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      601 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      961 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1021 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1081 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1141 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1201 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1261 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1321 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1381 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1441 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1501 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1561 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1621 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1681 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1741 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1801 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1861 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1921 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1981 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2041 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2101 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2161 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg
     2221 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag
     2281 ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa
     2341 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat
     2401 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
     2461 caagtgcagg tgccagaaca tttctctatc gataggtacc gagctcttac gcgtgctagc
     2521 ctgtcttcag ccaggtatct gaaagaccca gccacttttc ttagatcctg gcctaatgat
     2581 tccattgtaa tattcaaggc tttctaaaaa tccaaatggt aatttccttg aggatttgaa
     2641 caaactccac ttccaaattt tatttggctt agacatttta aacaggatag ccctgaagct
     2701 ctccatgctt catgacgcat tctaattgaa tccaaatgcc ttccctggta accattacca
     2761 aggtcaatta taatttttgg ccaaaagggg ttgcttagtt tccttttgat cagaaaatcc
     2821 ttttcaaatc tccttaacca gcgccaacat ctcttgccta acttgccata tggcaaatgt
     2881 cctctgggtt agtaaaaaca ggcctgcttt ttgtaagttg aagtttgcgg agagcagatg
     2941 gcagactcaa attaccttca agttgatgtg aggtctaagg aaagcctggc ctgcaagcgt
     3001 ccagatcagg ctccctcaca gaaccacact gagcatgaaa catgagcaaa actacctggt
     3061 gtacggcccc aggaaactgt acaccagcaa gtgaaggcct gcgggcagct ctaaatattc
     3121 ccacattgag tgaaaagata ggagataaag ccccatacga ctgatttgct gacactgtac
     3181 cttcccaaag acagaacaca tctcgtttta ttgttactat tgttcagcat ctgtccttcc
     3241 caacagacca tttagctcca taagagcttt tgttcaatgt aaattcctag tacagagcac
     3301 agctcctcgg ccatcacaga tgttaaatca cgtttgagta atgacttttc taaagtcaaa
     3361 tcatgtcatt tataaggaaa acaacatgta tagactacat ctgtaacaaa tgctaccctt
     3421 cttctgtaac cactcgagat ctgcgatctg catctcaatt agtcagcaac catagtcccg
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3541 cgctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggcattccg
     3661 gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
     3721 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
     3781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
     3841 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
     3901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
     3961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     4021 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
     4081 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
     4141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     4201 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
     4261 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
     4321 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     4381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     4441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
     4501 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     4561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     4621 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     4681 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     4741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     4801 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     4861 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     4921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     4981 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     5041 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     5101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     5161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     5221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     5281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga
     5341 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     5401 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     5461 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     5521 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     5581 gtggtaaaat cgataaggat ctggatccgt cgaccgatgc ccttgagagc cttcaaccca
     5641 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     5701 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     5761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttc
//
