LOCUS       sequence-461195-        3634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U1 snRNA tagged with 1 copy of Riboglow (truncated variant A with 5'
            antiPNA motif).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Wierzba AJ, Richards EM, Lennon SR, Batey RT, Palmer AE
  TITLE     Unveiling the promise of peptide nucleic acids as functional linkers
            for an RNA imaging platform.
  JOURNAL   RSC Chem Biol. 2024 Dec 19. doi: 10.1039/d4cb00274a.
  PUBMED    39759445
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RSC Chem 
            Biol. 2024 Dec 19. doi: 10.1039/d4cb00274a."
COMMENT     Sequence Label: env8-AD-5'antiPNA-U1
FEATURES             Location/Qualifiers
     source          1..3634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     968..988
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        970..988
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1748..1764)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1749..1765)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1789..1808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1790..1808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1818..1835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1818..1834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1827..1849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1976..2431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2063..2082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2273..2294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2457..2561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2562..2630,2631..3422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2780..2799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3593..3634,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcggta cccggggaga
     1021 tccggtaagg accagcttct ttgggagaga acagacgcag gggcgggagg gaaaaaggga
     1081 gaggcagacg tcacttcccc ttggcggctc tggcagcaga ttggtcggtt gagtggcaga
     1141 aaggcagacg gggactgggc aaggcactgt cggtgacatc acggacaggg cgacttctat
     1201 gtagatgagg cagcgcagag gctgctgctt cgccacttgc tgcttcacca cgaaggagtt
     1261 cccgtgccct gggagcgggt tcaggaccgc tgatcggaag tgagaatccc agctgtgtgt
     1321 cagggctgga aagggctcgg gagtgcgcgg ggcaagtgac cgtgtgtgta aagagtgagg
     1381 cgtatgaggc tgtgtcgggg cagaggccca agatctcata cttacctgat acaactttgg
     1441 cctaaaagcg tagtgggaaa gtgacgtgaa attcgtccag attacttgat acggttatac
     1501 tccgaatgcc acctaggccg caggggagat accatgatca cgaaggtggt tttcccaggg
     1561 cgaggcttat ccattgcact ccggatgtgc tgacccctgc gatttcccca aatgtgggaa
     1621 actcgactgc ataatttgtg gtagtggggg actgcgttcg cgctttcccc tgactttctg
     1681 gagtttcaaa agtagactgt acgctaaccg gatcctctag agtcgacctg caggcatgca
     1741 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     1801 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     1981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2401 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     2461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3601 ctttttttct gcgcgtaatc tgctgcttgc aaac
//
