LOCUS       sequence-461197-       10148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NPY-mNeonGreen fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10148)
  TITLE     expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-NPY-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..10148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2367..3545
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2598..3536
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3493..3513
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3872..4582
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    4604..5192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4657..4677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5075..5086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5716..5896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5922..5939)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5928..6152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6198..6626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6285..6304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6495..6516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6635..6655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6640..6969
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6820..6955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6882..6901
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7017..7064
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7083..7457
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7587..7708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7624..7643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7678..7697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7757..7773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7757..7773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7770..7792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7781..7797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7805..7811,7812..7829,7830..7835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7850..7871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7988..8005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8159..8747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8239..8258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8918..9709,9710..9778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9541..9560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9779..9883)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9958..9977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     2401 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     2461 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2521 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2581 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2641 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2701 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2761 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2821 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2881 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2941 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3001 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3061 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3121 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3181 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3241 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3301 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3361 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3421 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3481 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3541 cgtgaggaat tcggccatta cggccaccat gctaggtaac aagcgactgg ggctgtccgg
     3601 actgaccctc gccctgtccc tgctcgtgtg cctgggtgcg ctggccgagg cgtacccctc
     3661 caagccggac aacccgggcg aggacgcacc agcggaggac atggccagat actactcggc
     3721 gctgcgacac tacatcaacc tcatcaccag gcagagatat ggaaaacgat ccagcccaga
     3781 gacactgatt tcagacctct tgatgagaga aagcacagaa aatgttccca gaactcggct
     3841 tgaagaccct gcaatgtggg aattcggatc tatggtgagc aagggcgagg aggataacat
     3901 ggcctctctc ccagcgacac atgagttaca catctttggc tccatcaacg gtgtggactt
     3961 tgacatggtg ggtcagggca ccggcaatcc caatgatggt tatgaggagt taaacctgaa
     4021 gtccaccaag ggtgacctcc agttctcccc ctggattctg gtccctcata tcgggtatgg
     4081 cttccatcag tacctgccct accctgacgg gatgtcgcct ttccaggccg cgatggtaga
     4141 tggctccgga taccaagtcc atcgcacaat gcagtttgaa gatggtgcct cccttactgt
     4201 aaactaccgc tacacctacg agggaagcca catcaaagga gaggcccagg tgaaggggac
     4261 tggtttccct gctgacggtc ctgtgatgac caactcgctg accgctgcgg actggtgcag
     4321 gtcgaagaag acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac
     4381 cactggaaat ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc
     4441 aatggcggct aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctgaa
     4501 gcactccaag accgagctga acttcaagga gtggcaaaag gcctttaccg atgtgatggg
     4561 catggacgag ctgtacaagt aaggccgcct cggccgcggc cgcaatcaac ctctggatta
     4621 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4681 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4741 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4801 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4861 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4921 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4981 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     5041 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5101 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5161 gagtcggatc tccctttggg ccgcctcccc gcctgcctgc aggtttgtcg agacctagaa
     5221 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5281 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     5341 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5401 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     5461 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     5521 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     5581 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     5641 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     5701 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5881 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5941 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6001 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6061 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6121 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6181 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6601 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6721 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6781 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6841 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6901 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6961 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7021 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7081 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     7141 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     7201 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     7261 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     7321 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     7381 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     7441 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     7501 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7561 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7681 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7801 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8581 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9901 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9961 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10021 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10081 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    10141 tacgcgtt
//
