LOCUS       sequence-461207-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 5-HT6R coupled to the RGECO1 calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   DeCaen PG, Kimura LF
  TITLE     Methods to Assess Neuronal Primary Cilia Electrochemical Signaling.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70034. doi: 10.1002/jcp.70034.
  PUBMED    40227694
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70034. doi"; pages: " 
            10.1002/jcp.70034"
COMMENT     Sequence Label: pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2572..2581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3931..3987
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     4683..4702
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4726..5169
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     protein_bind    complement(5170..5194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5212..5800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5265..5285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5683..5694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5831..6330
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5866..5889)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6263..6282
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6347..6946
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6347..6366)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6843..6863
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7018..7251
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7323..7444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7360..7379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7414..7433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7484..7619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7639..7658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7640..7658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7668..7685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7668..7684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7677..7699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7826..8281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7913..7932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8123..8144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8307..8411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8412..8480,8481..9272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8630..8649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9443..10031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9932..9951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10185..10202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10319..10340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10355..10360,10361..10378,10379..10385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10393..10409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10398..10420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10417..10433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10417..10433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10452..10472
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10454..10472
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 gttccagagc ccggccctgt caacagtagc accccagcct ggggccccgg gccaccgcca
     2641 gccccagggg gcagcggctg ggtggccgct gcgctgtgcg tggtcatcgt gctgacggca
     2701 gccgccaatt cgctgctgat cgcgctcatc tgcacgcagc ccgcgctgcg caacacgtct
     2761 aacttcttcc tggtgtcgct cttcacgtcg gacctgatgg tgggattggt ggtgatgccc
     2821 ccagccatgc tgaacgcgct gtatgggcgc tgggtgctag ctcgcggcct ctgtctgctt
     2881 tggaccgcct tcgacgtgat gtgctgcagc gcctccattc tcaacctctg cctcatcagc
     2941 ctggaccgct acctgctcat cctctcgccg ctgcgctaca agctgcgcat gacagccccg
     3001 cgtgccctgg cgctcatcct gggtgcctgg agcctcgccg cgctggcctc cttcctgccc
     3061 ctcttgctgg gctggcatga actgggcaaa gctcgaacat ctgccccggg ccagtgccgc
     3121 ctattggcca gcctgcctta tgtcctcgtg gcgtccggcg tcaccttttt cctgccttcg
     3181 ggtgccatct gcttcaccta ctgcaggatc ttgctggctg cccgcaagca ggcggtgcaa
     3241 gtggcctcgc tcaccacggg cacggctacg gctggccagg ccttggaaac cttgcaggtg
     3301 cccaggacac cacgcccagg gatggagtct gctgacagta ggcgtctgac caccaagcat
     3361 agcaggaagg ccttgaaggc cagcctgacc ctcggcatcc tgctgagcat gttctttgtc
     3421 acctggctgc ccttctttgt ggccagcata gctcaggccg tatgtgactg catctctcca
     3481 ggtctcttcg atgtcctcac atggctggga tactgtaata gcaccatgaa ccctatcatc
     3541 tatcccctct tcatgcggga cttcaagagg gccctgggca ggttcgtgcc gtgtgtccac
     3601 tgtcccccgg agcaccgggc cagccccgcc tccccctcca tgtggacctc tcacagtggt
     3661 gccaggccag gcctcagcct gcagcaggtg ctgcccctgc ctctgccacc caactcagat
     3721 tcagactcag cttcaggggg cacctcgggc ctgcagctca cagcccagct tttgctgcct
     3781 ggagaggcga cccgggaccc cccgccaccc accagggccc ctactgtggt caacttcttc
     3841 gtcacagact ctgtggagcc tgagatacgg cagcatccac ttggttcccc catgaacggc
     3901 ggcggaggcg gcggaatggt cgactcttca cgtcgtaagt ggaataaggc aggtcacgca
     3961 gtcagagcta taggtcggct gagctcaccc gtggtttccg agcggatgta ccccgaggac
     4021 ggcgccctga agagcgagat caagaagggg ctgaggctga aggacggcgg ccactacgcc
     4081 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
     4141 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtgc
     4201 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaggtaca
     4261 ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     4321 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     4381 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc
     4441 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     4501 attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg
     4561 tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc
     4621 ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt cccccccgac
     4681 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcta cgcgtgacca actgactgaa
     4741 gagcagatcg cagaatttaa agaggctttc tccctatttg acaaggacgg ggatgggacg
     4801 ataacaacca aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca
     4861 gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacatt cgacttccct
     4921 gagttcctga cgatgatggc aagaaaaatg aatgacacag acagtgaaga ggaaattaga
     4981 gaagcgttcc gcgtgtttga taaggacggc aatggctaca tcggcgcagc agagcttcgc
     5041 cacgtgatga cagaccttgg agagaagtta acagatgagg aggttgatga aatgatcagg
     5101 gtagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca
     5161 gcgaagtaga cccagctttc ttgtacaaag tggtgataat cgaattccga taatcaacct
     5221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5581 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5761 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc
     5821 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5881 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5941 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     6001 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     6061 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     6121 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     6181 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     6241 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     6301 ttctcctctt cctcatctcc gggcctttcg acctcacgtg gccaccatga ccgagtacaa
     6361 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6421 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6481 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6541 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6601 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6661 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6721 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6781 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6841 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6901 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggta cctttaagac
     6961 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7021 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7261 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7321 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7441 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7501 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7561 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7621 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7681 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7741 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7801 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7861 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7921 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7981 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8041 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8101 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8161 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8221 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8281 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9841 aaagcgccac gcttcccgaa gagagaaagg cggacaggta tccggtaagc ggcagggtcg
     9901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10081 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10141 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10201 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10381 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10441 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
