LOCUS       sequence-461208-       11523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer-free Sparse.FRT10 p65.AD with ccdB for Gateway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Xu C, Luo L
  TITLE     The sparse driver system for in vivo single-cell labeling and 
            manipulation in Drosophila.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507.
  PUBMED    39677673
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507."
COMMENT     Sequence Label: pBP-Sparse.FRT10-p65.AD
FEATURES             Location/Qualifiers
     source          1..11523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    676..745
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(970..988)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1440..1511,1913..2186,2261..2915,2977..3292,3496..3627,
                     3698..4312)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5323..5339
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5323..5339
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(5783..6154)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             complement(6605..6625)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6656..6709)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6710..6757)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    7579..7713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7603..7622)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7657..7676
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7985..8005)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8135..8200
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    complement(8320..8367)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    8570..8694
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8735..9040)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8837..8856)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9382..10041)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9956..9975
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(10095..10101,10102..10119,10120..10125))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10150..10274)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10302..10320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10388..10492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10493..10561,10562..11353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10711..10730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt taccgtcgac
      661 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
      721 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      781 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      841 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      901 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaacat atgggcgcgc
      961 ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt
     1021 ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga
     1081 tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca
     1141 ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc
     1201 cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc
     1261 ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt
     1321 aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg
     1381 attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa
     1441 tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc
     1501 atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac
     1561 tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac
     1621 cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat
     1681 ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact
     1741 atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa
     1801 aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg
     1861 ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag
     1921 cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac
     1981 gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa
     2041 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg
     2101 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc
     2161 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg
     2221 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg
     2281 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc
     2341 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat
     2401 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac
     2461 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg
     2521 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt
     2581 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc
     2641 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg
     2701 atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg
     2761 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc
     2821 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg
     2881 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg
     2941 tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa
     3001 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga
     3061 gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga
     3121 tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg
     3181 gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct
     3241 gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg
     3301 ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt
     3361 agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat
     3421 agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa
     3481 cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc
     3541 acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc
     3601 tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa
     3661 taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt
     3721 ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac
     3781 gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc
     3841 gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct
     3901 ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc
     3961 gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt
     4021 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
     4081 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
     4141 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
     4201 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt
     4261 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     4321 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     4381 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     4441 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     4501 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     4561 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     4621 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     4681 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     4741 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     4801 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     4861 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     4921 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     4981 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     5041 acatttaatt tagaaaatgc ttggatttca ctggaactag ggcgcgcctc cggaacataa
     5101 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     5161 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     5221 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     5281 acgacaggag cacgatcatg cgcacccgtg gccagcggcc gctctagaac tagtggatct
     5341 aaacgagttt ttaagcaaac tcactccctg acaataaaaa cgcaattatc tttaaataaa
     5401 tatattctat ttattaacca agtagctaat atagctaaaa tcaatttgtt gctaacttaa
     5461 aactaacata attatgtatt agttatttac attcttttgg ttggttacaa ccaaataaat
     5521 aatatcactt ttaaaaactt aagccaggaa ctgaataatt ttctatttgg ctttagtcga
     5581 cggtatcgat aagcttttac ttgccgccgc ccagggggcc gtagcgggtg cggtactggc
     5641 tcacgcggtt gcgcaggcgc tgcacgcgct ggcgcagctc ggccacgcgg gtctccaggg
     5701 cggtgttctc gcgctccagg aaggcggcct cgatctccag actagtacct ccacctccac
     5761 ctccaccacc tcctccgcta gcggagctta tctggctcag caaagcggag aaatccatat
     5821 cagcgatgct ggagaaatcc tcatcgccgc tgaggaggcc attgggcaaa cccggggcac
     5881 ccagtggggc aggggctgga tcgggtggtc gctgtgcgcc ggtcaccagt cgcgtgatag
     5941 cctccgggta ttccatcagc atgggctctg tggtatgagg tgcgaccggg attccctgat
     6001 tcaagagctg ctgaaactcg ctgttgtcca cggatgcgag atcggtgaat acggcagggt
     6061 ctgtgctgtt acccagcagg gctcccaagt cttcgtcatc gaactgaagc tgcaagaggg
     6121 cctcgctcag ggttccttcg cccgcctgcg ttggtttcgg agcaggaggg gcaactgcct
     6181 gtgggggtcc aggcgccagc acggggactg gagcgggggc ctgggccaga gcactcacca
     6241 ttgcgggagc cggtgccgga gcgggcgcct gggggaggac ttgcggtggg gccggagcca
     6301 gtgccgatgc ctgggaaatt tgtccactgg ggaacaccat cgtggggaac tcatcatagt
     6361 taatcgtgct aagggaggaa gtgaacgggt agggctgcgg tgcgggtttg gggacgctgg
     6421 cactgctgcg cgaaggaacc gcaatacgac ggggcggggg gcgcgggtcc gtggggcccg
     6481 agaaggggga ctttttcatg atggacttga aggtttcata ggttcgcttc cgcttttctt
     6541 cgatacggtg acggtcatcc gtatcgggca ggtactggaa ctccatcggc gtggaaccca
     6601 attcgacctt tctcttcttt tttggaggct cgggaattaa ttccgcttta tccatgggcc
     6661 cggggttctc ctccacatcg ccgcaggtca gcaggctgcc gcggccctcg aagttcctat
     6721 actttctaga gaaaaggaac ttcggaatag gaacttcaag atcccccgga tcctcgcatg
     6781 gttttgccta attgtttcag atttatgggg cttacataca tacataatta ctttatacaa
     6841 cgaaaattac taaatagaat cgttcctgta agcaggactg acacatttgc ttgcgttgat
     6901 tgctattata tcgacctacc atagtcacat aaatatgtac atatataaat atgtatgtat
     6961 gtacaatcgc atgcttgacg gcagttaaga aatgaagtca ccatttcggt gagctggcaa
     7021 cgattatgct gtggccccga aaggaatgac tcatcgtccc agagaatcga taccaacggt
     7081 ggaaagccaa gtgcatgcca cactgcgtgc acagcctgct gctacacttg ccacaaacag
     7141 caggcggaaa agcgcagcag cggcccgaat cggggaaaat agaaagcgct aattatagac
     7201 gaagtgaacg gcagctctgc tttcccgcag tcgaaactcc gtgcaatgac cccgttcttt
     7261 caccacgaaa tgaatgatcg ctgggagaaa gaaaaggcaa agaaaaacat agtggggttt
     7321 tcttatttct gacaacactg aatctggccg attttattct tgtgtacaca acttatcgcc
     7381 gagttaactt ggcttttctg ctatacgtgt ctttgtggat aaaatacttt atggtttctc
     7441 gtacaatctt catcgttttc gtatgctttt cagtgttgca gtgaattttt tgttgcgatt
     7501 ggaagcgtaa acactgattt cgacggttac cccgcccacc aatgacgctc ccatcgagcg
     7561 ttgaagtggc gcgacgcaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     7621 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     7681 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     7741 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     7801 ggtatggctg attatgatct ctagtcaagg cactatacat caaatatttt tccataattt
     7861 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
     7921 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tcattggggt
     7981 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
     8041 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
     8101 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
     8161 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
     8221 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcctct
     8281 agcactagtg cgcgaattct ctagcttcaa aagcgctctg aagttcctat actttctaga
     8341 gaaaaggaac ttcggaatag gaacttcacg cgaggatcca agcagcatgt tttggtaccg
     8401 tttggtatgc gtcttgtgat tcaaagttgg cttattcaaa ggatattaac gaaggcagcg
     8461 gcacgtctgc tcggctcgaa cattcattca caactgatgc tctcagccac cccgcgccct
     8521 tttataccgc tgcgctcgat ccccgggcga gctcggccgg ccgtttatca ccactttgta
     8581 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     8641 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
     8701 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     8761 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     8821 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     8881 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     8941 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     9001 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     9061 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     9121 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     9181 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     9241 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     9301 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     9361 tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt actgttgtaa
     9421 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     9481 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     9541 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     9601 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     9661 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     9721 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     9781 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     9841 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     9901 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     9961 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
    10021 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta
    10081 actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10141 tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
    10201 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt
    10261 ttgtacaaac ttgtgataaa cgaattcttg aagacgaaag ggcctcgtga tacgcctatt
    10321 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    10381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    10501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    10741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    11041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    11221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    11281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    11341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    11401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11521 ttc
//
