LOCUS       sequence-461210-        6456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Korsten G, Osinga M, Pelle RA, Serweta AK, Hoogenberg B, Kampinga 
            HH, Kapitein LC
  TITLE     Nuclear poly-glutamine aggregates rupture the nuclear envelope and 
            hinder its repair.
  JOURNAL   J Cell Biol. 2024 Nov 4;223(11):e202307142. doi: 
            10.1083/jcb.202307142. Epub 2024 Aug 16.
  PUBMED    39150509
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-11-4"; volume: "223(11):e202307142. doi"; pages:
            " 10.1083/jcb.202307142. Epub 2024 Aug 16"
COMMENT     Sequence Label: emiRFP670-cGAS
FEATURES             Location/Qualifiers
     source          1..6456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3244..3365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3281..3300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3335..3354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3372..3827)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3509..3530)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3721..3740
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3854..3958
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3960..4317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3960..4151
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3983..4003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4168..4303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4352..5146
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4352..5146
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4406..4425)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5016..5035
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5334..5353)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5378..5425
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5754..6342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6243..6262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggcgga aggctccgtc gccaggcagc ctgacctctt gacctgcgaa
      661 catgaagaga tccacctcgc cggctcgatc cagccgcatg gcgcgcttct ggtcgtcagc
      721 gaacatgatc atcgcgtcat ccaggccagc gccaacgccg cggaatttct gaatctcgga
      781 agcgtactcg gcgttccgct cgccgagatc gacggcgatc tgttgatcaa gatcctgccg
      841 catctcgatc ccaccgccga aggcatgccg gtcgcggtgc gctgccggat cggcaatccc
      901 tctacggagt actgcggtct gatgcatcgg cctccggaag gcgggctgat catcgaactc
      961 gaacgtgccg gcccgtcgat cgatctgtca ggcacgctgg cgccggcgct ggagcggatc
     1021 cgcacggcgg gttcactgcg cgcgctgtgc gatgacaccg tgctgctgtt tcagcagtgc
     1081 accggctacg accgggtgat ggtgtatcgt ttcgatgagc aaggccacgg cctggtattc
     1141 tccgagtgcc atgtgcctgg gctcgaatcc tatttcggca accgctatcc gtcgtcgact
     1201 gtcccgcaga tggcgcggca gctgtacgtg cggcagcgcg tccgcgtgct ggtcgacgtc
     1261 acctatcagc cggtgccgct ggagccgcgg ctgtcgccgc tgaccgggcg cgatctcgac
     1321 atgtcgggct gcttcctgcg ctcgatgtcg ccgtgccatc tgcagttcct gaaggacatg
     1381 ggcgtgcgcg ccaccctggc ggtgtcgctg gtggtcggcg gcaagctgtg gggcctggtt
     1441 gtctgtcacc attatctgcc gcgcttcatc cgtttcgagc tgcgggcgat ctgcaaacgg
     1501 ctcgccgaaa ggatcgcgac gcggatcacc gcgcttgaga gctccggact cagatctatg
     1561 cagccttggc acggaaaggc catgcagaga gcttccgagg ccggagccac tgcccccaag
     1621 gcttccgcac ggaatgccag gggcgccccg atggatccca ccgagtctcc ggctgccccc
     1681 gaggccgccc tgcctaaggc gggaaagttc ggccccgcca ggaagtcggg atcccggcag
     1741 aaaaagagcg ccccggacac ccaggagagg ccgcccgtcc gcgcaactgg ggcccgcgcc
     1801 aaaaaggccc ctcagcgcgc ccaggacacg cagccgtctg acgccaccag cgcccctggg
     1861 gcagaggggc tggagcctcc tgcggctcgg gagccggctc tttccagggc tggttcttgc
     1921 cgccagaggg gcgcgcgctg ctccacgaag ccaagacctc cgcccgggcc ctgggacgtg
     1981 cccagccccg gcctgccggt ctcggccccc attctcgtac ggagggatgc ggcgcctggg
     2041 gcctcgaagc tccgggcggt tttggagaag ttgaagctca gccgcgatga tatctccacg
     2101 gcggcgggga tggtgaaagg ggttgtggac cacctgctgc tcagactgaa gtgcgactcc
     2161 gcgttcagag gcgtcgggct gctgaacacc gggagctact atgagcacgt gaagatttct
     2221 gcacctaatg aatttgatgt catgtttaaa ctggaagtcc ccagaattca actagaagaa
     2281 tattccaaca ctcgtgcata ttactttgtg aaatttaaaa gaaatccgaa agaaaatcct
     2341 ctgagtcagt ttttagaagg tgaaatatta tcagcttcta agatgctgtc aaagtttagg
     2401 aaaatcatta aggaagaaat taacgacatt aaagatacag atgtcatcat gaagaggaaa
     2461 agaggaggga gccctgctgt aacacttctt attagtgaaa aaatatctgt ggatataacc
     2521 ctggctttgg aatcaaaaag tagctggcct gctagcaccc aagaaggcct gcgcattcaa
     2581 aactggcttt cagcaaaagt taggaagcaa ctacgactaa agccatttta ccttgtaccc
     2641 aagcatgcaa aggaaggaaa tggtttccaa gaagaaacat ggcggctatc cttctctcac
     2701 atcgaaaagg aaattttgaa caatcatgga aaatctaaaa cgtgctgtga aaacaaagaa
     2761 gagaaatgtt gcaggaaaga ttgtttaaaa ctaatgaaat accttttaga acagctgaaa
     2821 gaaaggttta aagacaaaaa acatctggat aaattctctt cttatcatgt gaaaactgcc
     2881 ttctttcacg tatgtaccca gaaccctcaa gacagtcagt gggaccgcaa agacctgggc
     2941 ctctgctttg ataactgcgt gacatacttt cttcagtgcc tcaggacaga aaaacttgag
     3001 aattatttta ttcctgaatt caatctattc tctagcaact taattgacaa aagaagtaaa
     3061 gaatttctga caaagcaaat tgaatatgaa agaaacaatg agtttccagt ttttgatgaa
     3121 ttttgatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     3181 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3241 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3361 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3421 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3481 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3541 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3601 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3661 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3721 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3781 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3841 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3901 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3961 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4081 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4141 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     4321 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4381 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4441 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4501 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4561 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4621 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4681 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4741 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4801 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4861 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4921 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4981 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5041 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5101 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5161 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     5221 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5281 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     5341 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     5401 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     5461 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     5521 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5581 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5641 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5701 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5761 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5821 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5881 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6061 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6361 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6421 cctgattctg tggataaccg tattaccgcc atgcat
//
