LOCUS       sequence-461216-       13632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer VT028327 driven GAL4, gated by Sparse.FRT10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Xu C, Luo L
  TITLE     The sparse driver system for in vivo single-cell labeling and 
            manipulation in Drosophila.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507.
  PUBMED    39677673
REFERENCE   2  (bases 1 to 13632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507."
COMMENT     Sequence Label: VT028327-FRT10-STOP-FRT10-GAL4
FEATURES             Location/Qualifiers
     source          1..13632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    676..745
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(970..988)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1440..1511,1913..2186,2261..2915,2977..3292,3496..3627,
                     3698..4312)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5323..5339
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5323..5339
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(5597..8242)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(8243..8296)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(8297..8344)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    9166..9300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9190..9209)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9244..9263
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9572..9592)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9722..9787
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    complement(9907..9954)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    10157..10181
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12359..12383)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12411..12429)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12497..12601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12602..12670,12671..13462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12820..12839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt taccgtcgac
      661 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
      721 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      781 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      841 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      901 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaacat atgggcgcgc
      961 ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt
     1021 ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga
     1081 tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca
     1141 ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc
     1201 cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc
     1261 ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt
     1321 aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg
     1381 attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa
     1441 tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc
     1501 atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac
     1561 tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac
     1621 cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat
     1681 ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact
     1741 atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa
     1801 aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg
     1861 ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag
     1921 cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac
     1981 gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa
     2041 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg
     2101 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc
     2161 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg
     2221 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg
     2281 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc
     2341 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat
     2401 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac
     2461 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg
     2521 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt
     2581 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc
     2641 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg
     2701 atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg
     2761 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc
     2821 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg
     2881 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg
     2941 tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa
     3001 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga
     3061 gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga
     3121 tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg
     3181 gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct
     3241 gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg
     3301 ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt
     3361 agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat
     3421 agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa
     3481 cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc
     3541 acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc
     3601 tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa
     3661 taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt
     3721 ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac
     3781 gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc
     3841 gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct
     3901 ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc
     3961 gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt
     4021 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
     4081 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
     4141 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
     4201 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt
     4261 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     4321 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     4381 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     4441 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     4501 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     4561 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     4621 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     4681 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     4741 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     4801 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     4861 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     4921 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     4981 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     5041 acatttaatt tagaaaatgc ttggatttca ctggaactag ggcgcgcctc cggaacataa
     5101 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     5161 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     5221 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     5281 acgacaggag cacgatcatg cgcacccgtg gccagcggcc gctctagaac tagtggatct
     5341 aaacgagttt ttaagcaaac tcactccctg acaataaaaa cgcaattatc tttaaataaa
     5401 tatattctat ttattaacca agtagctaat atagctaaaa tcaatttgtt gctaacttaa
     5461 aactaacata attatgtatt agttatttac attcttttgg ttggttacaa ccaaataaat
     5521 aatatcactt ttaaaaactt aagccaggaa ctgaataatt ttctatttgg ctttagtcga
     5581 cggtatcgat aagcttctac tccttctttg ggttcggtgg agtatcctca tcatcaaaaa
     5641 gatagttata tacgtcgtcc atcgttgtgg tgttgaacat gccagtggtg ataccgaaag
     5701 cattgtaggc cgtctggtca gtccagcctg gggaaagggg cttgctgttg ttgccgtcgt
     5761 caatcttgga ggcagtaatt tcattattca taaagttatc atgcacattc gagctggcaa
     5821 tgggctggga caatgcctgg gagtttgtct gagtcgtgat caagtttgga ccgttggagc
     5881 tgttggtaaa cgtgaagctc aaggagctgt ccgcaatgtt accactctgg ttgaagttgg
     5941 ctccgccaaa caatgccgag ggcgtaagtg gcaccagact ctgcagttgc tgctgttgtc
     6001 ccaggaatgg ggtaacgcta cgatgcgacg gggtcgatcg gggtatagtg acaggggagt
     6061 tgcgggatgg tgggcggttg ctgagcagct tgaccagatc gctgaaggag gctccgcttt
     6121 tcagcggcac agggctcggg ccgacgctgc ctggacttac atacttcacc gatatgttgt
     6181 taacattttc ctcaggcagc gtgggatact gcgcgattgt agcggagccg acgatattct
     6241 tgatagcgga gccgttggaa ttattgtaac ttatgtgcgg gaggggaatt gcgcattgcg
     6301 aaagaaggaa tggggcgcac acctcttcca acacctggat gtacttttcg caagtctgaa
     6361 tcttgaatgt agcgagtttc ttcaacagca tcaacacggt gttaatttgc tgcaagagct
     6421 gtgctgtctc gttattctcg gcgttcgatt tggagttgct cagaagagtc ttgattggga
     6481 ccaacacggc gttgaacaaa tagtaggagc aattccaggc gaaataaggg gtcacattat
     6541 ggttgtccat ataggacgac accgacatca cggtgcgctg ggctgcgtcg gagagcatta
     6601 tgctgcacct ctttacctcg taggactgat ggtcgttctg atcttgctca agctgcgact
     6661 tcttctgcgt aaaatttgtg aaaaagtccc gcaaaacgta tataatcaga ctcagctgtt
     6721 tccacttcag ctcgaagcgg gtgaaactca gccaggggtg ttctttgagg agattagtca
     6781 gcgccgtagt gcttatgtcc atctgcagga acttgggtgc ctggcggctc acctcctcta
     6841 tctcattgca gatcatcagg catttcttgg cgcagatcgg actcttctcc gcggtcactg
     6901 tcttatccag ctcataaatc ttcgtaaaca cttgaagcaa gcgggccgtc tcaatgatac
     6961 cgtggtagat ggtcgggccc gtagttgtcc gttgaacgtc gtccacgctg gacgggaagg
     7021 aaatcgtgtt ctgactcagc tgaatgctgc gtccgtacaa gagggacagc tggatctccc
     7081 agctgtacac actccaccaa atcctccggc gctgttccag gatcgacgaa tcgctgaagc
     7141 tacttgggag gtcacgatta agtcccaggc taatcgccat ccgaatgctg aacgaatgaa
     7201 agttgtagga tgtattagtt ttctgacgcc actgggtata acgactcagc agatggaggg
     7261 ctgtcacgag gataatgctt ccactttcga agaccttcga ggtgaggtgc gacttggcgt
     7321 tttgatagta aaacacgtcg atatctgtgc tctcgccttc aatacaccaa gcgccgatgg
     7381 cgagaatgca gttgaagagg atctgccact ggtccttcga tgctatctca atctgattgt
     7441 tgtaaagcat catgagagta ggactatgca ctatggggca gtaggggtgg aagttgttaa
     7501 gatagctttg cagcaggcgg cttgtagtac tgcgactggc cagcgtgtaa cggtcggtga
     7561 tcattgtggg aagacgattg acattactgt tcgtgtaatt ttccggctta aaaccaatac
     7621 ttcgcaggat acacagcagg ctgccgtcgc caaagaaacc attgttattg gggtcggtct
     7681 tcaggaatgg gagtccgtcg ctcatatcat cctcctcgct ccagtcaaat ccatgcagtg
     7741 cgtcacgagg catgaagtcc agtggaatgg tgctgttgtc gtggtgggcc gcactgtcga
     7801 tcgataccgt cagttgccgt tgacccttat tggaactttc ttcactactg ctggtagccg
     7861 agatgcgatg ctgccgcaag gtaagtggca tatctgtctc caccgaggca aggcggtccg
     7921 tcacggcatc cttgttgacg ttatcctgaa caaacaggcc agtgagcagg gccttaatat
     7981 cttggagact atccatcttc agtatcatgt cgaggtcttc acggggaaaa atgaggagga
     8041 acaactgctc aaggcgctcc aggcgactct ccacttcagt caaatgagcg cgtgtcaaag
     8101 ggcttcgctt cgtctttggc gagtaccggc actcccaatt gttcttaaga catttggcgc
     8161 acttcggctt ttccttgctg cacttcagct tcttaagtcg gcatatgtca cacgcttgtt
     8221 caatactact cagcagcttc atgggcccgg ggttctcctc cacatcgccg caggtcagca
     8281 ggctgccgcg gccctcgaag ttcctatact ttctagagaa aaggaacttc ggaataggaa
     8341 cttcaagatc ccccggatcc tcgcatggtt ttgcctaatt gtttcagatt tatggggctt
     8401 acatacatac ataattactt tatacaacga aaattactaa atagaatcgt tcctgtaagc
     8461 aggactgaca catttgcttg cgttgattgc tattatatcg acctaccata gtcacataaa
     8521 tatgtacata tataaatatg tatgtatgta caatcgcatg cttgacggca gttaagaaat
     8581 gaagtcacca tttcggtgag ctggcaacga ttatgctgtg gccccgaaag gaatgactca
     8641 tcgtcccaga gaatcgatac caacggtgga aagccaagtg catgccacac tgcgtgcaca
     8701 gcctgctgct acacttgcca caaacagcag gcggaaaagc gcagcagcgg cccgaatcgg
     8761 ggaaaataga aagcgctaat tatagacgaa gtgaacggca gctctgcttt cccgcagtcg
     8821 aaactccgtg caatgacccc gttctttcac cacgaaatga atgatcgctg ggagaaagaa
     8881 aaggcaaaga aaaacatagt ggggttttct tatttctgac aacactgaat ctggccgatt
     8941 ttattcttgt gtacacaact tatcgccgag ttaacttggc ttttctgcta tacgtgtctt
     9001 tgtggataaa atactttatg gtttctcgta caatcttcat cgttttcgta tgcttttcag
     9061 tgttgcagtg aattttttgt tgcgattgga agcgtaaaca ctgatttcga cggttacccc
     9121 gcccaccaat gacgctccca tcgagcgttg aagtggcgcg acgcagatcc agacatgata
     9181 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     9241 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     9301 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     9361 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
     9421 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
     9481 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
     9541 gcaattctga aggaaagtca ttggggtctt ctacctttct cttctttttt ggaggagtag
     9601 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
     9661 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
     9721 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
     9781 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
     9841 agtaaggttc cttcacaaag atcctctagc actagtgcgc gaattctcta gcttcaaaag
     9901 cgctctgaag ttcctatact ttctagagaa aaggaacttc ggaataggaa cttcacgcga
     9961 ggatccaagc agcatgtttt ggtaccgttt ggtatgcgtc ttgtgattca aagttggctt
    10021 attcaaagga tattaacgaa ggcagcggca cgtctgctcg gctcgaacat tcattcacaa
    10081 ctgatgctct cagccacccc gcgccctttt ataccgctgc gctcgatccc cgggcgagct
    10141 cggccggccg tttatcacca ctttgtacaa gaaagctggg tcggcgcgcc cacccttcaa
    10201 cttctacggc aacggcattc atccccattg cgggtttacg cttcgcttaa tggcaaacaa
    10261 taattaaaag tgacaagtaa attagtttca gccgtcaact gattgcgatg agaatgccgg
    10321 cgaagttgtt tcgctaattt tttattagcc acagcgatct ggcggaaacc gatagggctt
    10381 agaagagttt ccggtttttg gccgcgagaa atttgcacct gctcgtgctg caggagcttt
    10441 tgttttcacc attcgctttc agcgatgttt tattcatgcg actattaact gattttatca
    10501 cgctacaaat atgcactaca gggcgtatgc ataataaatt ccagacaccg tttccaggca
    10561 ttgttaccgc taccgctttt accgctttta ctgcaacagc agctgctttc ctcgcaggac
    10621 gaatctgtgc gccgttttgc ggcaatcttc gacgcatttt tatatgccca cgctgtctta
    10681 tgaccttgtt tatccttgtc acatggccca aagtctttaa gcaccgacca caactttgat
    10741 gaactcgtaa aaaggaataa cgaataagtg gacatgaatt agtggcaaga gtcgaattaa
    10801 agttacccag taattgatgg gatcaattgc cagttcattc ataaagatca atggaatgat
    10861 ttgtttaaat taattactgg tatgttattt acacaacata aagtccatca aatattgatt
    10921 tagaaataaa acaaacagta gttaccgtaa gatctataaa atccaacgat tattatttga
    10981 aaaaaaagag cgcgccgaaa gtaggcggat ttttgcttcc atctaaaaaa acgaggagtc
    11041 tgtttgtgat gcctcttcat gaatgagtga ataaattcca aaatggttgt caaaaagcgt
    11101 cacagagaaa ttaatgatga cttttatacg cgagtaattc atggcatttt cataatacac
    11161 caacccacac acatgggata cctttaattt gatttataaa aaaaaaggaa aaatgtgaaa
    11221 aatatttaca tatatgtgaa cgcacgaagg aagagaatgc caaaaaggga gaggcacaaa
    11281 aaaacaggga aaatacccaa attatataaa catatttgtg tgtgagtgtg tgctcttatt
    11341 cttgtgactt ggcgactttt taaaaagctt ttttatgctc gtttgcatac actctgattt
    11401 tgatagcatt cgaagtcagt ttaacagtcg tcgtgcgatt tataacgcat aataagacaa
    11461 tcagaagcaa aaaaaaaaat gaagaaatgc gaccgcgtgc aaagaaaacc cgcaaataaa
    11521 tactatacat ataaatttgt ggcttcagtt cctctaaggc gataaagcaa attgacaatt
    11581 tccccgaccg gcgaccgctc tcggattttc ccggccagct tgtcagcttc ttacggcggt
    11641 cagcaaagtt gtcaaagtat ttccggcaca aaagtttcgc tttcgctctt attttaaaac
    11701 taaaaaccaa actaaacacg aacctggagc tcagcaaata aggctagaat tctattgaat
    11761 ggttgttggg ctgggtgggt caaaggtgaa gtcgggaaat tgtaaaagtt ttgtctgggc
    11821 gttaaaatcc tttctgagaa acgccatatt attgtagggt tctgatttgc atacgtattt
    11881 ttttcttagt tttttttctt agcttgactg agaagtaaga aaaactgcat agccatatat
    11941 tattgtaaat aagatgaaaa tcaaacaaag ataatcattt aatttttcta agcccaatct
    12001 catttaattc aaacttaaaa tcaagacagc aactaaatcg accttgatct taaaaggatt
    12061 tgcggttgtt ctacaactta atcgaagccc aggtcaatca ataaatataa agccacttag
    12121 cctgattact ctaacaactt ttgttttcgc aaaaggactt aaaaaaggag tgaagaggta
    12181 ccctattttt tttgttactt taccttacct taccttacct taccaatcaa tgccaaattg
    12241 aaaattcaat tttcgttaat cggcttaagc taacctcatg gcgtacctct ccggtatcat
    12301 aattttaact tttttccgcc gccacgacca aatgaaaata tcaagggggc ggccgcggag
    12361 cctgcttttt tgtacaaact tgtgataaac gaattcttga agacgaaagg gcctcgtgat
    12421 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    12481 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    12541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    12601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    12661 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    12721 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    12781 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    12841 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    12901 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    12961 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    13021 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    13081 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    13141 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    13201 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    13261 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    13321 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    13381 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    13441 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    13501 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    13561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    13621 caaaggatct tc
//
