LOCUS       sequence-46122-e       15069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 15069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pBT259_(pRosa26-G-tTA2)
FEATURES             Location/Qualifiers
     source          1..15069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1183..1486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1488..1765
                     /label=chicken beta-actin promoter
     intron          1767..2784
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2708..2730
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2792..2811
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2871..2880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2922..2943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    3170..3203
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3204..3237)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3238..3271)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3280..3313
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3320..3353)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3381..3485
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3487..3844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3487..3678
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3510..3530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3695..3830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3879..4673
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3879..4673
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3933..3952)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4543..4562
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4861..4880)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4905..4952
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(5144..5177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5297..5911,5912..5917,5918..5953,5954..5956,5957..5992,
                     5993..5995,5996..6031,6032..6040)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    6162..6283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6199..6218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6253..6272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    6303..10573
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10622..11115
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     11048..11067
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          11790..11855
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(11923..11940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11929..12153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12221..12241)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12221..12239)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12260..12276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12260..12276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12273..12295)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12284..12300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12308..12314,12315..12332,12333..12338))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12353..12374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12491..12508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12662..13250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12742..12761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13421..14212,14213..14281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14044..14063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14282..14386)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14412..14867)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14549..14570)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14761..14780
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14994..15016
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15008..15025
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15009..15025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15035..15054
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15035..15053
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagcct taattaaccg tttaaacaat tctgcaggaa tctagttatt aatagtaatc
     1141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1501 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1561 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg
     1621 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     1681 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     1741 taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg ccccgtgccc
     1801 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1861 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1921 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1981 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     2041 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2101 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     2161 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     2221 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2281 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     2341 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     2401 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     2461 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     2521 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2581 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2641 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2701 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     2761 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     2821 cattttggca aagaattgat ttgataccgc gggcccggga tccgatatcc gccaccatgg
     2881 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2941 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3001 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3061 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3121 acgacttctt caagtccgcc atgcccgaag gttggtatca aagatctccg aagttcctat
     3181 tctctagaaa gtataggaac ttcataactt cgtatagtac acattatacg aagttatata
     3241 acttcgtata ggatacttta tacgaagtta tggatctccg aagttcctat tctctagaaa
     3301 gtataggaac ttcggatcta taacttcgta tagcatacat tatacgaagt tattcaggtg
     3361 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3481 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3661 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3841 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     3901 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3961 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4021 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4081 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4141 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4201 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4261 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4321 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4381 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     4441 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4501 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4561 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4621 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4681 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4741 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4801 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     4861 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4921 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     4981 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     5041 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     5101 ggcaggccct gccatagcct caggttactc aggatctgga tctataactt cgtatagcat
     5161 acattatacg aagttatggt tacaagacag gtttaaggag accaatagaa actgggcatg
     5221 tggagacaga gaagactctt gggtttctga taggcactga ctctctctgc ctattggtct
     5281 attttcccac ccttagtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     5341 actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5401 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc
     5461 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5521 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
     5581 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5641 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5701 cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga
     5761 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     5821 gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     5881 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
     5941 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6001 cgctcttgac gattttgacc ttgacatgct ccccggatga gaattctaga taactgatca
     6061 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6121 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatttaaat tgggcgcgcc
     6301 ggctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg
     6361 ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc ccacagattt
     6421 tcggttttgt cgggaagttt tttaataggg gcaaatagga aaatggagga taggagtcat
     6481 ctggggttta tgcagcaaaa ctacaggtat attgcttgtg atccgcctcg gagatttcca
     6541 tgaggagata aagacatgtc acccgagttt atactctcct gcttagatcc tactacagta
     6601 tgaaatacag tgtygcgagg tagactatgt aagcagattt aatcatttta aagagcccag
     6661 tacttcatat ccatttctcc cgctccttct gcagccttat caaaaggtat ttagaacact
     6721 cattttagcc ccattttcat ttattatact ggcttatcca acccctagac agagcattgg
     6781 cattttccct ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac
     6841 atacaccaca aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct
     6901 tagtacactt tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc
     6961 ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag
     7021 atgtattgag aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt
     7081 ttctccatta taactgagct atwaccatta atggtttcag gtggatgtct cctcccccaa
     7141 tatacctgat gtatctacat attgccaggc tgatatttta agacatwaaa ggtatatttc
     7201 attattgagc cacatggtat tgattactgc tactaaaatt ttgtcattgt acacatctgt
     7261 aaaaggtggt tccttttgga atgcaaagtt caggtgtttg ttgtctttcc tgacctaagg
     7321 tcttgtgagc ttgtattttt tctatttaag cagtgctttc tcttggactg gcttgactca
     7381 tggcattcta cacgttattg ctggtctaaa tgtgattttg ccaagcttct tcaggaccta
     7441 taattttgct tgacttgtag ccaaacacaa gtaaaatgat taagcaacaa atgtatttgt
     7501 gaagcttggt ttttaggttg ttgtgttgtg tgtgcttgtg ctctataata atactatcca
     7561 ggggctggag aggtggctcg gagttcaaga gcacagactg ctcttccaga agtcctgagt
     7621 tcaattccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatgccctct
     7681 tctggtgtgt ctgaagacca caagtgtatt cacattaaat aaataatcct ccttcttctt
     7741 cttttttttt ttttaaagag aatwctgtct ccagtagaat tactgaagta atgaaatact
     7801 ttgtgtttgt tccaatatgg wagccaataa tcaaatactc ttwagcactg gaaatgtacc
     7861 aaggaactat tttatttaag tgwactgtgg acagaggagc cataactgca gacttgtggg
     7921 atacagaaga ccaatgcaga cttaatgtct tttctcttac actaagcaat aaagaaataa
     7981 aaattgaact tctagtatcc tatttgttaa actgctagct ttactaactt ttgtgcttca
     8041 tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc aagtaatgat
     8101 aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg tgcttgcctt
     8161 tatgccttaa tcccagcact ctggaggcag agacaggcag atctctgagt ttgagcccag
     8221 cctggtctac acatcaagtt ctatctagga tagccaggaa tacacacaga aaccctgttg
     8281 gggagggggg ctctgagatt tcataaaatt ataattgaag cattccctaa tgagccacta
     8341 tggatgtggc taaatccgtc tacctttctg atgagatttg ggtattattt tttctgtctc
     8401 tgctgttggt tgggtctttt gacactgtgg gctttcttaa agcctccttc cctgccatgt
     8461 ggtctcttgt ttgctactaa cttcccatgg cttaaatggc atggcttttt gccttctaag
     8521 ggcagctgct gagwtttgca gcctgatttc cagggtgggg ttgggaaatc tttcaaacac
     8581 taaaattgtc ctttaatttt tttttaaaaa atgggttata taataaacct cataaaatag
     8641 ttatgaggag tgaggtggac taatattaat gagtccctcc cctataaaag agctattaag
     8701 gctttttgtc ttatactaac ttttttttta aatgtggtat ctttagaacc aagggtctta
     8761 gagttttagt atacagaaac tgttgcatcg cttaatcaga ttttctagtt tcaaatccag
     8821 agaatccaaa ttcttcacag ccaaagtcaa attaagaatt tctgacttta atgttatttg
     8881 ctactgtgaa tataaaatga tagcttttcc tgaggcaggg tctcactatg tatctctgcc
     8941 tgatctgcaa caagatatgt agactaaagt tctgcctgct tttgtctcct gaatactaag
     9001 gttaaaatgt agtaatactt ttggaacttg caggtcagat tcttttatag gggacacact
     9061 aagggagctt gggtgatagt tggtaaatgt gtttaagtga tgaaaacttg aattattatc
     9121 accgcaacct actttttaaa aaaaaaagcc aggcctgtta gagcatgcta agggatccct
     9181 aggacttgct gagcacacaa gagtagtact tggcaggctc ctggtgagag catatttcaa
     9241 aaaacaaggc agacaaccaa gaaactacag taaggttacc tgtctttaac catctgcata
     9301 tacacaggga tattaaaata ttccaaataa tatttcattc aagttttccc ccatcaaatt
     9361 gggacatgga tttctccggt gaataggcag agttggaaac taaacaaatg ttggttttgt
     9421 gatttgtgaa attgttttca agtgatagtt aaagcccatg agatacagaa caaagctgct
     9481 atttcgaggt ctcttggtta tactcagaag cacttctttg ggtttccctg cactatcctg
     9541 atcatgtgct aggcctwcct taggctgatt gttgttcaaa taacttaagt ttcctgtcag
     9601 gtgatgtcat atgatttcat atatcaaggc aaaacatgtt atatatgtta aacatttgka
     9661 cttaatgtga aagttaggtc tttgtgggtt ttgattttaa tttcaaaacc tgagctaaat
     9721 aagtcatttt acatgtctta catttggtga attgtatatt gtggtttgca ggcaagactc
     9781 tctgacctag taaccctcct atagagcact ttgctgggtc acaagtctag gagtcaagca
     9841 tttcaccttg aagttgagac gttttgttag tgtatactag ttatatgttg gaggacatgt
     9901 ttatccagaa gatattcagg actatttttg actgggctaa ggaattgatt ctgattagca
     9961 ctgttagtga gcattgagtg gcctttaggc ttgaattgga gtcacttgta tatctcaaat
    10021 aatgctggcc ttttttwaaa agcccttgtt ctttatcacc ctgttttcta cataattttt
    10081 gttcaaagaa atacttgttt ggatctcctt ttgacaacaa tagcatgttt tcaagccata
    10141 ttttttttcc tttttttttt tttttttggt ttttcgagac agggtttctc tgtatagccc
    10201 tggctgtcct ggaactcact ttgtagacca ggctggcctc gaactcagaa atccgcctgc
    10261 ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc acgcctggct aagttggata
    10321 ttttgtatat aactataacc aatactaact ccactgggtg gatttttaat tcagtcagta
    10381 gtcttaagtg gtctttattg gcccttatta aaatctactg ttcactctaa cagaggctgt
    10441 tggactagtg gsactaagca acttcctacg gatatactag cagataaggg tcagggatag
    10501 aaactagtct agcgttttgt atacctacca gcttatacta ccttgttctg atagaaatat
    10561 ttaggacatc tagcttatcg atccgtcgac ggtatcgata agcttgatat cgaattctac
    10621 cgggtagggg aggcgctttt ccaaggcagt ctgagcatgc gcttagcagc cccgctggca
    10681 cttggcgcta cacaagtggc ctytggcctc gcacacattc cacatccacc ggtaggcgcc
    10741 aaccggctcc gttctttggt ggccccttcg cgccaccttc twctcctccc ctagtcagga
    10801 agttcccccc cgccccgcag ctcgcgtcgt saggacgtga caaatggaag tagcacgtct
    10861 cactagtctc gtcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
    10921 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
    10981 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
    11041 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
    11101 tctccgggcc tttcgacctg caggtcctcg ccatggatcc tgatgatgtt ttgttattct
    11161 tctaatcttt tgtatggaaa acttttcttc gtaccacggg actaaacctg gttatgtaga
    11221 ttccattcaa aaaggtatac aaaagccaaa atctggtaca caaggaaatt atgacgatga
    11281 ttggaaaggg ttttatagta ccgacaataa atacgacgct gcgggatact ctgtagataa
    11341 tgaaaacccg ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc caggactgac
    11401 gaaggttctc gcactaaaag tggataatgc cgaaactatt aagaaagagt taggtttaag
    11461 tctcactgaa ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa ggttcggtga
    11521 tggtgcttcg cgtgtagtgc tcagccttcc cttcgctgag gggagttcta gcgttgaata
    11581 tattaataac tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta attttgaaac
    11641 ccgtggaaaa cgtggccaag atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa
    11701 tcgtgtcagg cgatctcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
    11761 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
    11821 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
    11881 gggagcagtg gtggaatgca gatcctagag ctcgctgatc agcctcgact gtgccttcta
    11941 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
    12001 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
    12061 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
    12121 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
    12181 gctcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg
    12241 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12301 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    12361 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    12421 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    12481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    12541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    12601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    12661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    12721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    12781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    12841 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    12901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    12961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13081 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    13141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    13321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    13381 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    13441 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    13501 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    13561 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    13621 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    13681 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    13741 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    13801 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    13861 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    13921 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    13981 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    14041 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    14101 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    14161 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    14221 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    14281 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    14341 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    14401 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    14461 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    14521 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    14581 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    14641 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    14701 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    14761 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    14821 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
    14881 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    14941 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    15001 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
    15061 ggagctcca
//
