LOCUS       sequence-461242-       12484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sparse.FRT10 GAL4 for HACK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Xu C, Luo L
  TITLE     The sparse driver system for in vivo single-cell labeling and 
            manipulation in Drosophila.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507.
  PUBMED    39677673
REFERENCE   2  (bases 1 to 12484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507."
COMMENT     Sequence Label: pHACK-Sparse.FRT10-GAL4
FEATURES             Location/Qualifiers
     source          1..12484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    676..745
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_RNA        complement(1192..1267)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1288..1708)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(1709..1784)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1804..2600)
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     protein_bind    complement(2616..2649)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(2659..3003)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    4038..4071
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4249..4959
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4394..4412)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    5079..5200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5264..5297
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5569..8214)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(8215..8268)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(8269..8316)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    9138..9272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9544..9564)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9694..9759
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    complement(9879..9926)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(10683..11120)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    complement(11160..11193)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11263..11281)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11349..11453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11454..11522,11523..12314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11672..11691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt taccgtcgac
      661 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
      721 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      781 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggtgc
      841 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      901 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaacat attaattaag
      961 catacgcatt aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac
     1021 acacgtcaga tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat
     1081 acataccaaa ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac
     1141 gaaacatttt tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact
     1201 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
     1261 ctaaaactcc agctttctca gaatacacga cgttaaattg aaaataggtc tatatatacg
     1321 aactgagtct ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc
     1381 tgcttggcga gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg
     1441 taaaattaat gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa
     1501 aacgtatgaa atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg
     1561 tatttttttt aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct
     1621 gtaaaataaa tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga
     1681 gtaggagcaa tcacaggtga gcaaaaaagc accgactcgg tgccactttt tcaagttgat
     1741 aacggactag ccttatttta acttgctatt tctagctcta aaacgttgtg gtacgctgca
     1801 catcgaagtt cacccggata tctttcctat atatactgta ctcttgcaga atatgtactt
     1861 gggaatcata aggatgcgaa ggacgtacgc gctgtgtctg taactaaggg gttgctttcg
     1921 ggtacctacc atgccaatga tgggactacc gccggatagg aatccctttt gggtgcacac
     1981 cgtcacctcc agttccgtct gcctgagttc ggcaatggaa atcaggtact caaaggatgc
     2041 atcgtagacg gggttgcagt tgtccttgat cacgctcgtc ttgcgtttcg actccttggt
     2101 gcgtccaggc aacagataca gcttaacata cggatcgggg atattgctgg gatcgcgaag
     2161 tggtatcttc tggattttgt gtatggtcac gtctagtttt tgacgctggg cgctgtagcg
     2221 gatcgacaac tgcattcgtc caaggccatg ttcgccgggt gaggaggtgg agtctggaaa
     2281 gcggtgggtc agcacagagg tggccagctc gctgatgggt ttttcgctgg aggtggaggc
     2341 cgacatggcc ggggagattt tcgtagccgc cacaggatct tcttcactga tgggcgattc
     2401 ggcggacaaa cgcttgctgg atgcctgaga ttcctaaaag caatggcaaa ttagattcaa
     2461 ttaaaacaaa aattgtttat ataacaaagt aaaagaaaaa aaaaaagaat cacagattct
     2521 ccccgacggg gaattgaacc ccggtctccc gcgtgacagg cggggatact aaccactata
     2581 ctatcgagga cgttgaaaat gaattcagat cactagaagt tcctatactt tctagagaat
     2641 aggaacttca ctagtctatt actctttttt tgggtttggt ggggtatctt catcatcgaa
     2701 tagatagtta tatacatcat ccattgtagt ggtattaaac atccctgtag tgattccaaa
     2761 cgcgttatac gcagtttggt ccgtccaacc aggtgacagt ggttttgaat tattaccatc
     2821 atcaatttta ctagccgtga tttcattatt catgaagtta tcatgaacgt tagaggaggc
     2881 aattggttgt gaaagcgctt gagaatttgt ttgagttgtt atgaggttcg gaccgttgct
     2941 actgttagtg aaagtgaagg acaatgagct atcagcaata ttcccacttt gattaaaatt
     3001 ggcgccacca aacaaagcag acggggtcag tggcactaat gattgcagct gttgctgttg
     3061 ccctagaaaa ggcgtgactg agcgatgcga aggtgtgctt cttggtattg tcactggaga
     3121 gttacgagag ggtggacggt tagataacag cttgactaga tcactgaaac ttgctcctga
     3181 tttcaatggc acaggtgaag gccctactga gccaggagaa acatatttaa cactgatatt
     3241 gttgacattt tcctccggaa gagtagggta ttgggcgata gttgcagaac cgacaatatt
     3301 tttaatggcg ctaccattac tattgttata actgatatgc ggtaatggga ttgcacactg
     3361 tgataacaga aacggcgcac atacctcttc cagtacttga atgtattttt cacaagtctg
     3421 gattttaaaa gtggccagtt tttttaatag catcagaaca gtgttaattt gttgtaataa
     3481 ttgtgcggtc tcgttattct cagcattcga ttttgagttt gagagtagag tctttatggg
     3541 tactaggact gcattgaaca agtaataaga acaattccag gcaaaatatg gggtgacatt
     3601 atgattgtcc atatagctac ttacagacat aacagttctt tgtgctgcat cgcttaacat
     3661 gatggagcat cgtttaactt cataactttg atgatcattt tgatcctgtt ctagttgtga
     3721 ctttttctgg gtaaaattag tgaaaaaatc tcttaataca taaatgataa gagacaactg
     3781 tttccacttc agttcgaatc ttgtaaagga tagccaaggg tgttccttca acaaattggt
     3841 tagagcggtg gtggaaatat ccatttgtaa aaactttggt gcctgtctcg aaacctcctc
     3901 aatctcatta caaatcatca agcatttttt tgcacatata ggactttttt ctgcagttac
     3961 tgttttgtct agttcataga tttttgtgaa aacttgtaag agccttgctg tttcaatgat
     4021 gccatgacta gtgatctata acttcgtata atgtatgcta tacgaagtta tgacgtcatt
     4081 caattagaga ctaattcaat tagagctaat tcaattagcg ccggagtata aatagaggcg
     4141 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     4201 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atcggcccat ggtgagcaag
     4261 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4321 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4381 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4441 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4501 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4561 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4621 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaaa
     4681 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4741 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4801 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4861 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4921 cgccactcca ccggcggcat ggacgagctg tacaagtaag cgccgcgact ctgatcataa
     4981 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5041 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5101 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5161 attctagttg tggtttgtcc aaactcatca atgtatctta gctggatctt ataaaaaatt
     5221 caattatact tgctaaacta acggttaatc gtcgagtggt accataactt cgtataatgt
     5281 atgctatacg aagttatgca tgcggtggat ctaaacgagt ttttaagcaa actcactccc
     5341 tgacaataaa aacgcaatta tctttaaata aatatattct atttattaac caagtagcta
     5401 atatagctaa aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt
     5461 acattctttt ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccagg
     5521 aactgaataa ttttctattt ggctttagtc gacggtatcg ataagcttct actccttctt
     5581 tgggttcggt ggagtatcct catcatcaaa aagatagtta tatacgtcgt ccatcgttgt
     5641 ggtgttgaac atgccagtgg tgataccgaa agcattgtag gccgtctggt cagtccagcc
     5701 tggggaaagg ggcttgctgt tgttgccgtc gtcaatcttg gaggcagtaa tttcattatt
     5761 cataaagtta tcatgcacat tcgagctggc aatgggctgg gacaatgcct gggagtttgt
     5821 ctgagtcgtg atcaagtttg gaccgttgga gctgttggta aacgtgaagc tcaaggagct
     5881 gtccgcaatg ttaccactct ggttgaagtt ggctccgcca aacaatgccg agggcgtaag
     5941 tggcaccaga ctctgcagtt gctgctgttg tcccaggaat ggggtaacgc tacgatgcga
     6001 cggggtcgat cggggtatag tgacagggga gttgcgggat ggtgggcggt tgctgagcag
     6061 cttgaccaga tcgctgaagg aggctccgct tttcagcggc acagggctcg ggccgacgct
     6121 gcctggactt acatacttca ccgatatgtt gttaacattt tcctcaggca gcgtgggata
     6181 ctgcgcgatt gtagcggagc cgacgatatt cttgatagcg gagccgttgg aattattgta
     6241 acttatgtgc gggaggggaa ttgcgcattg cgaaagaagg aatggggcgc acacctcttc
     6301 caacacctgg atgtactttt cgcaagtctg aatcttgaat gtagcgagtt tcttcaacag
     6361 catcaacacg gtgttaattt gctgcaagag ctgtgctgtc tcgttattct cggcgttcga
     6421 tttggagttg ctcagaagag tcttgattgg gaccaacacg gcgttgaaca aatagtagga
     6481 gcaattccag gcgaaataag gggtcacatt atggttgtcc atataggacg acaccgacat
     6541 cacggtgcgc tgggctgcgt cggagagcat tatgctgcac ctctttacct cgtaggactg
     6601 atggtcgttc tgatcttgct caagctgcga cttcttctgc gtaaaatttg tgaaaaagtc
     6661 ccgcaaaacg tatataatca gactcagctg tttccacttc agctcgaagc gggtgaaact
     6721 cagccagggg tgttctttga ggagattagt cagcgccgta gtgcttatgt ccatctgcag
     6781 gaacttgggt gcctggcggc tcacctcctc tatctcattg cagatcatca ggcatttctt
     6841 ggcgcagatc ggactcttct ccgcggtcac tgtcttatcc agctcataaa tcttcgtaaa
     6901 cacttgaagc aagcgggccg tctcaatgat accgtggtag atggtcgggc ccgtagttgt
     6961 ccgttgaacg tcgtccacgc tggacgggaa ggaaatcgtg ttctgactca gctgaatgct
     7021 gcgtccgtac aagagggaca gctggatctc ccagctgtac acactccacc aaatcctccg
     7081 gcgctgttcc aggatcgacg aatcgctgaa gctacttggg aggtcacgat taagtcccag
     7141 gctaatcgcc atccgaatgc tgaacgaatg aaagttgtag gatgtattag ttttctgacg
     7201 ccactgggta taacgactca gcagatggag ggctgtcacg aggataatgc ttccactttc
     7261 gaagaccttc gaggtgaggt gcgacttggc gttttgatag taaaacacgt cgatatctgt
     7321 gctctcgcct tcaatacacc aagcgccgat ggcgagaatg cagttgaaga ggatctgcca
     7381 ctggtccttc gatgctatct caatctgatt gttgtaaagc atcatgagag taggactatg
     7441 cactatgggg cagtaggggt ggaagttgtt aagatagctt tgcagcaggc ggcttgtagt
     7501 actgcgactg gccagcgtgt aacggtcggt gatcattgtg ggaagacgat tgacattact
     7561 gttcgtgtaa ttttccggct taaaaccaat acttcgcagg atacacagca ggctgccgtc
     7621 gccaaagaaa ccattgttat tggggtcggt cttcaggaat gggagtccgt cgctcatatc
     7681 atcctcctcg ctccagtcaa atccatgcag tgcgtcacga ggcatgaagt ccagtggaat
     7741 ggtgctgttg tcgtggtggg ccgcactgtc gatcgatacc gtcagttgcc gttgaccctt
     7801 attggaactt tcttcactac tgctggtagc cgagatgcga tgctgccgca aggtaagtgg
     7861 catatctgtc tccaccgagg caaggcggtc cgtcacggca tccttgttga cgttatcctg
     7921 aacaaacagg ccagtgagca gggccttaat atcttggaga ctatccatct tcagtatcat
     7981 gtcgaggtct tcacggggaa aaatgaggag gaacaactgc tcaaggcgct ccaggcgact
     8041 ctccacttca gtcaaatgag cgcgtgtcaa agggcttcgc ttcgtctttg gcgagtaccg
     8101 gcactcccaa ttgttcttaa gacatttggc gcacttcggc ttttccttgc tgcacttcag
     8161 cttcttaagt cggcatatgt cacacgcttg ttcaatacta ctcagcagct tcatgggccc
     8221 ggggttctcc tccacatcgc cgcaggtcag caggctgccg cggccctcga agttcctata
     8281 ctttctagag aaaaggaact tcggaatagg aacttcaaga tcccccggat cctcgcatgg
     8341 ttttgcctaa ttgtttcaga tttatggggc ttacatacat acataattac tttatacaac
     8401 gaaaattact aaatagaatc gttcctgtaa gcaggactga cacatttgct tgcgttgatt
     8461 gctattatat cgacctacca tagtcacata aatatgtaca tatataaata tgtatgtatg
     8521 tacaatcgca tgcttgacgg cagttaagaa atgaagtcac catttcggtg agctggcaac
     8581 gattatgctg tggccccgaa aggaatgact catcgtccca gagaatcgat accaacggtg
     8641 gaaagccaag tgcatgccac actgcgtgca cagcctgctg ctacacttgc cacaaacagc
     8701 aggcggaaaa gcgcagcagc ggcccgaatc ggggaaaata gaaagcgcta attatagacg
     8761 aagtgaacgg cagctctgct ttcccgcagt cgaaactccg tgcaatgacc ccgttctttc
     8821 accacgaaat gaatgatcgc tgggagaaag aaaaggcaaa gaaaaacata gtggggtttt
     8881 cttatttctg acaacactga atctggccga ttttattctt gtgtacacaa cttatcgccg
     8941 agttaacttg gcttttctgc tatacgtgtc tttgtggata aaatacttta tggtttctcg
     9001 tacaatcttc atcgttttcg tatgcttttc agtgttgcag tgaatttttt gttgcgattg
     9061 gaagcgtaaa cactgatttc gacggttacc ccgcccacca atgacgctcc catcgagcgt
     9121 tgaagtggcg cgacgcagat ccagacatga taagatacat tgatgagttt ggacaaacca
     9181 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     9241 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     9301 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     9361 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt ccataatttt
     9421 cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
     9481 attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt cattggggtc
     9541 ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
     9601 atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
     9661 ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
     9721 tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
     9781 aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatcctcta
     9841 gcactagtgc gcgaattctc tagcttcaaa agcgctctga agttcctata ctttctagag
     9901 aaaaggaact tcggaatagg aacttcacgc gaggatccaa gcagggacaa ttggatctcc
     9961 caagagtaga cagaccacca aattcggcgt ctttgttcca gaatgctgct atcactgaag
    10021 gacgagggga ggtccctatt caagcccaat gatatggcca ttcttatgga aaagctgtga
    10081 aaattatagc tagtatttgt tttctgcctc cactgtgtat atcgcgacag aagatgtagg
    10141 gctgtcacca aaattatgga acctgactcg aagaccttgc tcgtcaaatg agatttagca
    10201 ttttgatagt aaaaaacatc tatatcagta gattccccct ctatacacca ggctccaatg
    10261 gctaatatgc agttaaaaag gatttgccat tgatccttcg acgcgatttc aatctggtta
    10321 ttatacaaca tcattagcgt cggtgagtgc acgatagggc agtaggggtg aaaattattg
    10381 agataacttt gaagtaaacg ggatgttgtg gatctagaag ccaacgtgta tctatccgta
    10441 atcatggtcg ggagcctgtt aacgttagag ttcgtgtaat tttccggttt aaagccaata
    10501 gatcgaagaa tacataagag agaaccgtcg ccaaagaacc cattattgtt ggggtccgtt
    10561 ttcaggaagg gcaagccatc cgacatgtca tcctcttcag accaatcaaa tccatgaaga
    10621 gcatccctgg gcataaaatc caacggaatt gtggagttat catgatgagc tgccgagtca
    10681 atcgatacag tcaactgtct ttgacctttg ttactactct cttccgatga tgatgtcgca
    10741 cttattctat gctgtctcaa tgttagaggc atatcagtct ccactgaagc caatctatct
    10801 gtgacggcat ctttattcac attatcttgt acaaataatc ctgttaacaa tgcttttata
    10861 tcctgtaaag aatccatttt caaaatcatg tcaaggtctt ctcgaggaaa aatcagtaga
    10921 aatagctgtt ccagtctttc tagccttgat tccacttctg tcagatgtgc cctagtcagc
    10981 ggagaccttt tggttttggg agagtagcga cactcccagt tgttcttcag acacttggcg
    11041 cacttcggtt tttctttgga gcacttgagc tttttaagtc ggcaaatatc gcatgcttgt
    11101 tcgatagaag acagtagctt tcatcattag gatccagatc tattaccctg ttatccctag
    11161 aagttcctat actttctaga gaataggaac ttcagatctc gcgttacgta gaagtttcta
    11221 ttctctagaa agtatagaaa cttcgtactt gaagacgaaa gggcctcgtg atacgcctat
    11281 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
    11341 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11401 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    11461 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11521 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    11581 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    11641 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    11701 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11761 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11821 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11881 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11941 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    12001 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    12061 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    12121 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    12181 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    12241 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12301 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    12361 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12421 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12481 cttc
//
