LOCUS       sequence-461244-       10897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sparse.FRT10 p65.AD for HACK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Xu C, Luo L
  TITLE     The sparse driver system for in vivo single-cell labeling and 
            manipulation in Drosophila.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507.
  PUBMED    39677673
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507."
COMMENT     Sequence Label: pHACK-Sparse.FRT10-p65.AD
FEATURES             Location/Qualifiers
     source          1..10897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    676..745
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_RNA        complement(1192..1267)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1288..1708)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(1709..1784)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1804..2600)
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     protein_bind    complement(2616..2649)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(2659..3003)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    4038..4071
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4249..4959
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4394..4412)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    5079..5200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5264..5297
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5755..6126)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             complement(6577..6597)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6628..6681)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6682..6729)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    7551..7685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7957..7977)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8107..8172
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    complement(8292..8339)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(9096..9533)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    complement(9573..9606)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9676..9694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9762..9866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9867..9935,9936..10727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10085..10104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt taccgtcgac
      661 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
      721 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      781 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggtgc
      841 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      901 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaacat attaattaag
      961 catacgcatt aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac
     1021 acacgtcaga tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat
     1081 acataccaaa ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac
     1141 gaaacatttt tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact
     1201 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
     1261 ctaaaactcc agctttctca gaatacacga cgttaaattg aaaataggtc tatatatacg
     1321 aactgagtct ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc
     1381 tgcttggcga gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg
     1441 taaaattaat gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa
     1501 aacgtatgaa atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg
     1561 tatttttttt aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct
     1621 gtaaaataaa tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga
     1681 gtaggagcaa tcacaggtga gcaaaaaagc accgactcgg tgccactttt tcaagttgat
     1741 aacggactag ccttatttta acttgctatt tctagctcta aaacgttgtg gtacgctgca
     1801 catcgaagtt cacccggata tctttcctat atatactgta ctcttgcaga atatgtactt
     1861 gggaatcata aggatgcgaa ggacgtacgc gctgtgtctg taactaaggg gttgctttcg
     1921 ggtacctacc atgccaatga tgggactacc gccggatagg aatccctttt gggtgcacac
     1981 cgtcacctcc agttccgtct gcctgagttc ggcaatggaa atcaggtact caaaggatgc
     2041 atcgtagacg gggttgcagt tgtccttgat cacgctcgtc ttgcgtttcg actccttggt
     2101 gcgtccaggc aacagataca gcttaacata cggatcgggg atattgctgg gatcgcgaag
     2161 tggtatcttc tggattttgt gtatggtcac gtctagtttt tgacgctggg cgctgtagcg
     2221 gatcgacaac tgcattcgtc caaggccatg ttcgccgggt gaggaggtgg agtctggaaa
     2281 gcggtgggtc agcacagagg tggccagctc gctgatgggt ttttcgctgg aggtggaggc
     2341 cgacatggcc ggggagattt tcgtagccgc cacaggatct tcttcactga tgggcgattc
     2401 ggcggacaaa cgcttgctgg atgcctgaga ttcctaaaag caatggcaaa ttagattcaa
     2461 ttaaaacaaa aattgtttat ataacaaagt aaaagaaaaa aaaaaagaat cacagattct
     2521 ccccgacggg gaattgaacc ccggtctccc gcgtgacagg cggggatact aaccactata
     2581 ctatcgagga cgttgaaaat gaattcagat cactagaagt tcctatactt tctagagaat
     2641 aggaacttca ctagtctatt actctttttt tgggtttggt ggggtatctt catcatcgaa
     2701 tagatagtta tatacatcat ccattgtagt ggtattaaac atccctgtag tgattccaaa
     2761 cgcgttatac gcagtttggt ccgtccaacc aggtgacagt ggttttgaat tattaccatc
     2821 atcaatttta ctagccgtga tttcattatt catgaagtta tcatgaacgt tagaggaggc
     2881 aattggttgt gaaagcgctt gagaatttgt ttgagttgtt atgaggttcg gaccgttgct
     2941 actgttagtg aaagtgaagg acaatgagct atcagcaata ttcccacttt gattaaaatt
     3001 ggcgccacca aacaaagcag acggggtcag tggcactaat gattgcagct gttgctgttg
     3061 ccctagaaaa ggcgtgactg agcgatgcga aggtgtgctt cttggtattg tcactggaga
     3121 gttacgagag ggtggacggt tagataacag cttgactaga tcactgaaac ttgctcctga
     3181 tttcaatggc acaggtgaag gccctactga gccaggagaa acatatttaa cactgatatt
     3241 gttgacattt tcctccggaa gagtagggta ttgggcgata gttgcagaac cgacaatatt
     3301 tttaatggcg ctaccattac tattgttata actgatatgc ggtaatggga ttgcacactg
     3361 tgataacaga aacggcgcac atacctcttc cagtacttga atgtattttt cacaagtctg
     3421 gattttaaaa gtggccagtt tttttaatag catcagaaca gtgttaattt gttgtaataa
     3481 ttgtgcggtc tcgttattct cagcattcga ttttgagttt gagagtagag tctttatggg
     3541 tactaggact gcattgaaca agtaataaga acaattccag gcaaaatatg gggtgacatt
     3601 atgattgtcc atatagctac ttacagacat aacagttctt tgtgctgcat cgcttaacat
     3661 gatggagcat cgtttaactt cataactttg atgatcattt tgatcctgtt ctagttgtga
     3721 ctttttctgg gtaaaattag tgaaaaaatc tcttaataca taaatgataa gagacaactg
     3781 tttccacttc agttcgaatc ttgtaaagga tagccaaggg tgttccttca acaaattggt
     3841 tagagcggtg gtggaaatat ccatttgtaa aaactttggt gcctgtctcg aaacctcctc
     3901 aatctcatta caaatcatca agcatttttt tgcacatata ggactttttt ctgcagttac
     3961 tgttttgtct agttcataga tttttgtgaa aacttgtaag agccttgctg tttcaatgat
     4021 gccatgacta gtgatctata acttcgtata atgtatgcta tacgaagtta tgacgtcatt
     4081 caattagaga ctaattcaat tagagctaat tcaattagcg ccggagtata aatagaggcg
     4141 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     4201 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atcggcccat ggtgagcaag
     4261 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4321 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4381 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4441 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4501 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4561 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4621 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaaa
     4681 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4741 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4801 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4861 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4921 cgccactcca ccggcggcat ggacgagctg tacaagtaag cgccgcgact ctgatcataa
     4981 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5041 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5101 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5161 attctagttg tggtttgtcc aaactcatca atgtatctta gctggatctt ataaaaaatt
     5221 caattatact tgctaaacta acggttaatc gtcgagtggt accataactt cgtataatgt
     5281 atgctatacg aagttatgca tgcggtggat ctaaacgagt ttttaagcaa actcactccc
     5341 tgacaataaa aacgcaatta tctttaaata aatatattct atttattaac caagtagcta
     5401 atatagctaa aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt
     5461 acattctttt ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccagg
     5521 aactgaataa ttttctattt ggctttagtc gacggtatcg ataagctttt acttgccgcc
     5581 gcccaggggg ccgtagcggg tgcggtactg gctcacgcgg ttgcgcaggc gctgcacgcg
     5641 ctggcgcagc tcggccacgc gggtctccag ggcggtgttc tcgcgctcca ggaaggcggc
     5701 ctcgatctcc agactagtac ctccacctcc acctccacca cctcctccgc tagcggagct
     5761 tatctggctc agcaaagcgg agaaatccat atcagcgatg ctggagaaat cctcatcgcc
     5821 gctgaggagg ccattgggca aacccggggc acccagtggg gcaggggctg gatcgggtgg
     5881 tcgctgtgcg ccggtcacca gtcgcgtgat agcctccggg tattccatca gcatgggctc
     5941 tgtggtatga ggtgcgaccg ggattccctg attcaagagc tgctgaaact cgctgttgtc
     6001 cacggatgcg agatcggtga atacggcagg gtctgtgctg ttacccagca gggctcccaa
     6061 gtcttcgtca tcgaactgaa gctgcaagag ggcctcgctc agggttcctt cgcccgcctg
     6121 cgttggtttc ggagcaggag gggcaactgc ctgtgggggt ccaggcgcca gcacggggac
     6181 tggagcgggg gcctgggcca gagcactcac cattgcggga gccggtgccg gagcgggcgc
     6241 ctgggggagg acttgcggtg gggccggagc cagtgccgat gcctgggaaa tttgtccact
     6301 ggggaacacc atcgtgggga actcatcata gttaatcgtg ctaagggagg aagtgaacgg
     6361 gtagggctgc ggtgcgggtt tggggacgct ggcactgctg cgcgaaggaa ccgcaatacg
     6421 acggggcggg gggcgcgggt ccgtggggcc cgagaagggg gactttttca tgatggactt
     6481 gaaggtttca taggttcgct tccgcttttc ttcgatacgg tgacggtcat ccgtatcggg
     6541 caggtactgg aactccatcg gcgtggaacc caattcgacc tttctcttct tttttggagg
     6601 ctcgggaatt aattccgctt tatccatggg cccggggttc tcctccacat cgccgcaggt
     6661 cagcaggctg ccgcggccct cgaagttcct atactttcta gagaaaagga acttcggaat
     6721 aggaacttca agatcccccg gatcctcgca tggttttgcc taattgtttc agatttatgg
     6781 ggcttacata catacataat tactttatac aacgaaaatt actaaataga atcgttcctg
     6841 taagcaggac tgacacattt gcttgcgttg attgctatta tatcgaccta ccatagtcac
     6901 ataaatatgt acatatataa atatgtatgt atgtacaatc gcatgcttga cggcagttaa
     6961 gaaatgaagt caccatttcg gtgagctggc aacgattatg ctgtggcccc gaaaggaatg
     7021 actcatcgtc ccagagaatc gataccaacg gtggaaagcc aagtgcatgc cacactgcgt
     7081 gcacagcctg ctgctacact tgccacaaac agcaggcgga aaagcgcagc agcggcccga
     7141 atcggggaaa atagaaagcg ctaattatag acgaagtgaa cggcagctct gctttcccgc
     7201 agtcgaaact ccgtgcaatg accccgttct ttcaccacga aatgaatgat cgctgggaga
     7261 aagaaaaggc aaagaaaaac atagtggggt tttcttattt ctgacaacac tgaatctggc
     7321 cgattttatt cttgtgtaca caacttatcg ccgagttaac ttggcttttc tgctatacgt
     7381 gtctttgtgg ataaaatact ttatggtttc tcgtacaatc ttcatcgttt tcgtatgctt
     7441 ttcagtgttg cagtgaattt tttgttgcga ttggaagcgt aaacactgat ttcgacggtt
     7501 accccgccca ccaatgacgc tcccatcgag cgttgaagtg gcgcgacgca gatccagaca
     7561 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7621 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7681 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     7741 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa
     7801 ggcactatac atcaaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
     7861 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
     7921 acttagcaat tctgaaggaa agtcattggg gtcttctacc tttctcttct tttttggagg
     7981 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
     8041 aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag ttccataggt
     8101 tggaatctaa aatacacaaa caattagaat cagtagttta acacattata cacttaaaaa
     8161 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
     8221 acagaagtaa ggttccttca caaagatcct ctagcactag tgcgcgaatt ctctagcttc
     8281 aaaagcgctc tgaagttcct atactttcta gagaaaagga acttcggaat aggaacttca
     8341 cgcgaggatc caagcaggga caattggatc tcccaagagt agacagacca ccaaattcgg
     8401 cgtctttgtt ccagaatgct gctatcactg aaggacgagg ggaggtccct attcaagccc
     8461 aatgatatgg ccattcttat ggaaaagctg tgaaaattat agctagtatt tgttttctgc
     8521 ctccactgtg tatatcgcga cagaagatgt agggctgtca ccaaaattat ggaacctgac
     8581 tcgaagacct tgctcgtcaa atgagattta gcattttgat agtaaaaaac atctatatca
     8641 gtagattccc cctctataca ccaggctcca atggctaata tgcagttaaa aaggatttgc
     8701 cattgatcct tcgacgcgat ttcaatctgg ttattataca acatcattag cgtcggtgag
     8761 tgcacgatag ggcagtaggg gtgaaaatta ttgagataac tttgaagtaa acgggatgtt
     8821 gtggatctag aagccaacgt gtatctatcc gtaatcatgg tcgggagcct gttaacgtta
     8881 gagttcgtgt aattttccgg tttaaagcca atagatcgaa gaatacataa gagagaaccg
     8941 tcgccaaaga acccattatt gttggggtcc gttttcagga agggcaagcc atccgacatg
     9001 tcatcctctt cagaccaatc aaatccatga agagcatccc tgggcataaa atccaacgga
     9061 attgtggagt tatcatgatg agctgccgag tcaatcgata cagtcaactg tctttgacct
     9121 ttgttactac tctcttccga tgatgatgtc gcacttattc tatgctgtct caatgttaga
     9181 ggcatatcag tctccactga agccaatcta tctgtgacgg catctttatt cacattatct
     9241 tgtacaaata atcctgttaa caatgctttt atatcctgta aagaatccat tttcaaaatc
     9301 atgtcaaggt cttctcgagg aaaaatcagt agaaatagct gttccagtct ttctagcctt
     9361 gattccactt ctgtcagatg tgccctagtc agcggagacc ttttggtttt gggagagtag
     9421 cgacactccc agttgttctt cagacacttg gcgcacttcg gtttttcttt ggagcacttg
     9481 agctttttaa gtcggcaaat atcgcatgct tgttcgatag aagacagtag ctttcatcat
     9541 taggatccag atctattacc ctgttatccc tagaagttcc tatactttct agagaatagg
     9601 aacttcagat ctcgcgttac gtagaagttt ctattctcta gaaagtatag aaacttcgta
     9661 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     9721 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10081 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10141 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10201 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10261 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10321 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10381 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10441 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10501 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10561 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10621 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10681 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10861 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
