LOCUS       sequence-461246-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sparse.FRT10 GAL4.DBD for HACK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Xu C, Luo L
  TITLE     The sparse driver system for in vivo single-cell labeling and 
            manipulation in Drosophila.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507.
  PUBMED    39677673
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 4:2024.12.02.626507. doi: 
            10.1101/2024.12.02.626507."
COMMENT     Sequence Label: pHACK-Sparse.FRT10-GAL4.DBD
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    676..745
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_RNA        complement(1192..1267)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1288..1708)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(1709..1784)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1804..2600)
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     protein_bind    complement(2616..2649)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(2659..3003)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    4038..4071
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4249..4959
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4394..4412)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    5079..5200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5264..5297
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5572..6012)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             complement(6199..6252)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6253..6300)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    7122..7256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7528..7548)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7678..7743
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    complement(7863..7910)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(8667..9104)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    complement(9144..9177)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9247..9265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9333..9437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9438..9506,9507..10298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9656..9675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt taccgtcgac
      661 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
      721 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      781 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggtgc
      841 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      901 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaacat attaattaag
      961 catacgcatt aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac
     1021 acacgtcaga tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat
     1081 acataccaaa ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac
     1141 gaaacatttt tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact
     1201 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
     1261 ctaaaactcc agctttctca gaatacacga cgttaaattg aaaataggtc tatatatacg
     1321 aactgagtct ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc
     1381 tgcttggcga gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg
     1441 taaaattaat gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa
     1501 aacgtatgaa atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg
     1561 tatttttttt aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct
     1621 gtaaaataaa tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga
     1681 gtaggagcaa tcacaggtga gcaaaaaagc accgactcgg tgccactttt tcaagttgat
     1741 aacggactag ccttatttta acttgctatt tctagctcta aaacgttgtg gtacgctgca
     1801 catcgaagtt cacccggata tctttcctat atatactgta ctcttgcaga atatgtactt
     1861 gggaatcata aggatgcgaa ggacgtacgc gctgtgtctg taactaaggg gttgctttcg
     1921 ggtacctacc atgccaatga tgggactacc gccggatagg aatccctttt gggtgcacac
     1981 cgtcacctcc agttccgtct gcctgagttc ggcaatggaa atcaggtact caaaggatgc
     2041 atcgtagacg gggttgcagt tgtccttgat cacgctcgtc ttgcgtttcg actccttggt
     2101 gcgtccaggc aacagataca gcttaacata cggatcgggg atattgctgg gatcgcgaag
     2161 tggtatcttc tggattttgt gtatggtcac gtctagtttt tgacgctggg cgctgtagcg
     2221 gatcgacaac tgcattcgtc caaggccatg ttcgccgggt gaggaggtgg agtctggaaa
     2281 gcggtgggtc agcacagagg tggccagctc gctgatgggt ttttcgctgg aggtggaggc
     2341 cgacatggcc ggggagattt tcgtagccgc cacaggatct tcttcactga tgggcgattc
     2401 ggcggacaaa cgcttgctgg atgcctgaga ttcctaaaag caatggcaaa ttagattcaa
     2461 ttaaaacaaa aattgtttat ataacaaagt aaaagaaaaa aaaaaagaat cacagattct
     2521 ccccgacggg gaattgaacc ccggtctccc gcgtgacagg cggggatact aaccactata
     2581 ctatcgagga cgttgaaaat gaattcagat cactagaagt tcctatactt tctagagaat
     2641 aggaacttca ctagtctatt actctttttt tgggtttggt ggggtatctt catcatcgaa
     2701 tagatagtta tatacatcat ccattgtagt ggtattaaac atccctgtag tgattccaaa
     2761 cgcgttatac gcagtttggt ccgtccaacc aggtgacagt ggttttgaat tattaccatc
     2821 atcaatttta ctagccgtga tttcattatt catgaagtta tcatgaacgt tagaggaggc
     2881 aattggttgt gaaagcgctt gagaatttgt ttgagttgtt atgaggttcg gaccgttgct
     2941 actgttagtg aaagtgaagg acaatgagct atcagcaata ttcccacttt gattaaaatt
     3001 ggcgccacca aacaaagcag acggggtcag tggcactaat gattgcagct gttgctgttg
     3061 ccctagaaaa ggcgtgactg agcgatgcga aggtgtgctt cttggtattg tcactggaga
     3121 gttacgagag ggtggacggt tagataacag cttgactaga tcactgaaac ttgctcctga
     3181 tttcaatggc acaggtgaag gccctactga gccaggagaa acatatttaa cactgatatt
     3241 gttgacattt tcctccggaa gagtagggta ttgggcgata gttgcagaac cgacaatatt
     3301 tttaatggcg ctaccattac tattgttata actgatatgc ggtaatggga ttgcacactg
     3361 tgataacaga aacggcgcac atacctcttc cagtacttga atgtattttt cacaagtctg
     3421 gattttaaaa gtggccagtt tttttaatag catcagaaca gtgttaattt gttgtaataa
     3481 ttgtgcggtc tcgttattct cagcattcga ttttgagttt gagagtagag tctttatggg
     3541 tactaggact gcattgaaca agtaataaga acaattccag gcaaaatatg gggtgacatt
     3601 atgattgtcc atatagctac ttacagacat aacagttctt tgtgctgcat cgcttaacat
     3661 gatggagcat cgtttaactt cataactttg atgatcattt tgatcctgtt ctagttgtga
     3721 ctttttctgg gtaaaattag tgaaaaaatc tcttaataca taaatgataa gagacaactg
     3781 tttccacttc agttcgaatc ttgtaaagga tagccaaggg tgttccttca acaaattggt
     3841 tagagcggtg gtggaaatat ccatttgtaa aaactttggt gcctgtctcg aaacctcctc
     3901 aatctcatta caaatcatca agcatttttt tgcacatata ggactttttt ctgcagttac
     3961 tgttttgtct agttcataga tttttgtgaa aacttgtaag agccttgctg tttcaatgat
     4021 gccatgacta gtgatctata acttcgtata atgtatgcta tacgaagtta tgacgtcatt
     4081 caattagaga ctaattcaat tagagctaat tcaattagcg ccggagtata aatagaggcg
     4141 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     4201 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atcggcccat ggtgagcaag
     4261 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4321 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4381 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4441 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4501 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4561 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4621 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaaa
     4681 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4741 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4801 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4861 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4921 cgccactcca ccggcggcat ggacgagctg tacaagtaag cgccgcgact ctgatcataa
     4981 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5041 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5101 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5161 attctagttg tggtttgtcc aaactcatca atgtatctta gctggatctt ataaaaaatt
     5221 caattatact tgctaaacta acggttaatc gtcgagtggt accataactt cgtataatgt
     5281 atgctatacg aagttatgca tgcggtggat ctaaacgagt ttttaagcaa actcactccc
     5341 tgacaataaa aacgcaatta tctttaaata aatatattct atttattaac caagtagcta
     5401 atatagctaa aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt
     5461 acattctttt ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccagg
     5521 aactgaataa ttttctattt ggctttagtc gacggtatcg ataagctttt acgataccgt
     5581 cagttgccgt tgacccttat tggaactttc ttcactactg ctggtagccg agatgcgatg
     5641 ctgccgcaag gtaagtggca tatctgtctc caccgaggca aggcggtccg tcacggcatc
     5701 cttgttgacg ttatcctgaa caaacaggcc agtgagcagg gccttaatat cttggagact
     5761 atccatcttc agtatcatgt cgaggtcttc acggggaaaa atgaggagga acaactgctc
     5821 aaggcgctcc aggcgactct ccacttcagt caaatgagcg cgtgtcaaag ggcttcgctt
     5881 cgtctttggc gagtaccggc actcccaatt gttcttaaga catttggcgc acttcggctt
     5941 ttccttgctg cacttcagct tcttaagtcg gcatatgtca cacgcttgtt caatactact
     6001 cagcagcttc atactagtac ctccacctcc acctccacca cctcctccgc tagccttgcc
     6061 gccgcccagg gggccgtagc gggtctcgta ctggctcacc tcgttctcca ggcgctgcac
     6121 ctcctgctcc agctcggcca cctcggtgcg cagggcggtg ttgcgctggc gcaggaaggc
     6181 ggcgcggatc tccagcatgg gcccggggtt ctcctccaca tcgccgcagg tcagcaggct
     6241 gccgcggccc tcgaagttcc tatactttct agagaaaagg aacttcggaa taggaacttc
     6301 aagatccccc ggatcctcgc atggttttgc ctaattgttt cagatttatg gggcttacat
     6361 acatacataa ttactttata caacgaaaat tactaaatag aatcgttcct gtaagcagga
     6421 ctgacacatt tgcttgcgtt gattgctatt atatcgacct accatagtca cataaatatg
     6481 tacatatata aatatgtatg tatgtacaat cgcatgcttg acggcagtta agaaatgaag
     6541 tcaccatttc ggtgagctgg caacgattat gctgtggccc cgaaaggaat gactcatcgt
     6601 cccagagaat cgataccaac ggtggaaagc caagtgcatg ccacactgcg tgcacagcct
     6661 gctgctacac ttgccacaaa cagcaggcgg aaaagcgcag cagcggcccg aatcggggaa
     6721 aatagaaagc gctaattata gacgaagtga acggcagctc tgctttcccg cagtcgaaac
     6781 tccgtgcaat gaccccgttc tttcaccacg aaatgaatga tcgctgggag aaagaaaagg
     6841 caaagaaaaa catagtgggg ttttcttatt tctgacaaca ctgaatctgg ccgattttat
     6901 tcttgtgtac acaacttatc gccgagttaa cttggctttt ctgctatacg tgtctttgtg
     6961 gataaaatac tttatggttt ctcgtacaat cttcatcgtt ttcgtatgct tttcagtgtt
     7021 gcagtgaatt ttttgttgcg attggaagcg taaacactga tttcgacggt taccccgccc
     7081 accaatgacg ctcccatcga gcgttgaagt ggcgcgacgc agatccagac atgataagat
     7141 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7201 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7261 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     7321 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata
     7381 catcaaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta
     7441 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa
     7501 ttctgaagga aagtcattgg ggtcttctac ctttctcttc ttttttggag gagtagaatg
     7561 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt
     7621 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta
     7681 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt
     7741 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
     7801 aggttccttc acaaagatcc tctagcacta gtgcgcgaat tctctagctt caaaagcgct
     7861 ctgaagttcc tatactttct agagaaaagg aacttcggaa taggaacttc acgcgaggat
     7921 ccaagcaggg acaattggat ctcccaagag tagacagacc accaaattcg gcgtctttgt
     7981 tccagaatgc tgctatcact gaaggacgag gggaggtccc tattcaagcc caatgatatg
     8041 gccattctta tggaaaagct gtgaaaatta tagctagtat ttgttttctg cctccactgt
     8101 gtatatcgcg acagaagatg tagggctgtc accaaaatta tggaacctga ctcgaagacc
     8161 ttgctcgtca aatgagattt agcattttga tagtaaaaaa catctatatc agtagattcc
     8221 ccctctatac accaggctcc aatggctaat atgcagttaa aaaggatttg ccattgatcc
     8281 ttcgacgcga tttcaatctg gttattatac aacatcatta gcgtcggtga gtgcacgata
     8341 gggcagtagg ggtgaaaatt attgagataa ctttgaagta aacgggatgt tgtggatcta
     8401 gaagccaacg tgtatctatc cgtaatcatg gtcgggagcc tgttaacgtt agagttcgtg
     8461 taattttccg gtttaaagcc aatagatcga agaatacata agagagaacc gtcgccaaag
     8521 aacccattat tgttggggtc cgttttcagg aagggcaagc catccgacat gtcatcctct
     8581 tcagaccaat caaatccatg aagagcatcc ctgggcataa aatccaacgg aattgtggag
     8641 ttatcatgat gagctgccga gtcaatcgat acagtcaact gtctttgacc tttgttacta
     8701 ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag aggcatatca
     8761 gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc ttgtacaaat
     8821 aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat catgtcaagg
     8881 tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct tgattccact
     8941 tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta gcgacactcc
     9001 cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt gagcttttta
     9061 agtcggcaaa tatcgcatgc ttgttcgata gaagacagta gctttcatca ttaggatcca
     9121 gatctattac cctgttatcc ctagaagttc ctatactttc tagagaatag gaacttcaga
     9181 tctcgcgtta cgtagaagtt tctattctct agaaagtata gaaacttcgt acttgaagac
     9241 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9301 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9661 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9961 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10441 accccgtaga aaagatcaaa ggatcttc
//
