LOCUS       sequence-461249-       11852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express mCherry-53BP1 fragment to monitor 53BP1 foci..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-CMV-mCherry-53BP1
FEATURES             Location/Qualifiers
     source          1..11852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             922..1102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(2856..3563)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3131..3150)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3400..3418
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(3597..3800)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3626..3646)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3801..4104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4203..4320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4613..4657)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4842..5075
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5568..5693
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5740..6373
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6541..6675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6565..6584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6619..6638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7100..7120)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7250..7315
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8449..8778)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8463..8598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8763..8783
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8853..8870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9024..9612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9104..9123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9783..10574,10575..10643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10406..10425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10644..10748)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10816..10834
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10872..10894)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10994..11013
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11227..11246
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cacaaaatct gcagtcaact taattgaatg cagtattcta cttgtgttaa taacaaactt
       61 gttttgacaa attgtgataa taaggattgt tttctactct aatgatacac atttctatgt
      121 tcccttttta aaagtaaatt acatttataa tattgataat cttcaataat atttaattag
      181 ttttatcaag ttccaacaat tatttgaagt catatttaag gttatattgt atagtcttgt
      241 tgccagaaaa atttatccct tgctttatgc atttgaatcc ctttctaacc aagttacttg
      301 ttaaaacttg ttaaatgaca actgttattc ttatcctcac ttctcttctt cttgaagcta
      361 ttcaggtttg caaacaattt cttctctcaa tctactcagt atttactata taaaagggta
      421 atttgttact tagcataatg tccataactt tgatatattc gactgagtca tagggtgagg
      481 agtaaatgga atttgtgtca gaagatcata gagtcgtaac taacggatgc tgtaattgtg
      541 ggaaaattac ttcaacatct ttccctctca gtttcctctt gtgttaaatg gggataatag
      601 taatcatctc ttatgatatt tgaggattaa ttaatatctg tcagaatagt aaattagata
      661 agtttatact ttaaactata aatcattata taaatgttag taaacttaat gtaatcctta
      721 ggatctggga ttagccaggt atgtgcagaa tcgtgagtat ctctggtggc ttcttccaag
      781 tacttctgca gctttgcctg tgtgctccac tcctagttca attcctgcta aatctattat
      841 aatgtcaagg cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
      901 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
      961 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1021 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1081 tctggtctaa ccagagagac ccagtacaag caaaaagcag ctgcttatat gcagcatctc
     1141 tcgacgattt tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     1201 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     1261 gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt
     1321 agctgctgta ttgctacttg tgattgctcc atgtttttct aggtctcgag tcattcaccg
     1381 gtgttgtctc cactctcaca ggggctcaca aactctcctg cccctactgc accaggtttt
     1441 actgtggcag acttgcgacc tcgcttggca ggggaccgtt cctcttcaga agtgataagt
     1501 tttcttttgc ctgagagaac tcccatggag gcacgaggac tttctgtgat ctttcgggta
     1561 ggggttgtgc tgctgctact ggaggcagta ggggtggctg gggagctgac gttactgcgc
     1621 cgtttccgct tcccttccac caaattgtct aagctgatat ctgctgcctt tgtaagaggt
     1681 gttactgctt cataggggcc aagcccatac tgctctctca gtctgtttcc ttgctccaag
     1741 gacaggatga cagccattcg cttataccac tttctttggc cttctttttc aatgctgtag
     1801 tacagttccc cagactcctt cctatgtcct ttcaccactc ctgcactgaa atactcatcc
     1861 tccgagaggg ccgtcacttc agtgtccagc gggatggggt cacataacag aatgtctttg
     1921 cccaacacat cacattcgta cccatcatca aagagcaatt tatacttccc agctccgaca
     1981 tctcgtgtga ttttcccaga gtaaaagtag ccattggatg accacttggc tacaacacgg
     2041 agccctacaa agctatttcc tggagaggag gcatctaagc catcagaagg gccagcagca
     2101 gcctggaaag gaatttctgg agagtcacta cgacgcaaag caccagcacc cacatctgtt
     2161 cgtctggtgg agtctggcac tcggggcacg acacggctga aggatttatc atctggtgac
     2221 aagttaggtg aaatgtcctc tatgcccaag gggccaggca cagctgtttc tctggttcca
     2281 gtggtccgag aaggtgggcg gccccttcgc ccacgcccac gaggcgtgac tggagccttc
     2341 cctccctgtc tgatgccaag gcctgcatca ccatcctcct cacacactgg cgtccctgtc
     2401 tgactgaccc cttttctagg actcagtttt cctgggcctc ctcgggagct gggtaaggca
     2461 aaatctgcgg gttctgtccc gctggttttc cctctgagtg gtccggctcc tttccctgag
     2521 cttccactgc tgtgcatagc tgagagactt gtcccacttg atgtgcggtg taagctggat
     2581 gccttggagg agaaggagct gatatccccc aggtcacctg aggagccccc agtctgtgaa
     2641 ggggaaactt cagtttcaca ctcctgacac tctacaattg gctcttcagt ctcctcagtt
     2701 acttttcttt ctacttctgt tccatccaca taatacacat ctgtaatgac acgagtgaca
     2761 agtgtgcgta cttcccggat tgttctcatg tgacgatgta agacatggcc atgtggaggc
     2821 tgaggcatat caaactcttc ttctccctgg gatcccttgt acagctcgtc catgccgccg
     2881 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
     2941 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
     3001 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
     3061 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
     3121 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
     3181 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
     3241 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
     3301 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
     3361 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
     3421 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
     3481 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
     3541 tcctcctcgc ccttgctcac catactagcc cgcgggatct gacggttcac taaaccagct
     3601 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     3661 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     3721 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     3781 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     3841 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     3901 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     3961 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     4021 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     4081 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     4141 gtaattgatt actatacgcg tatcagccaa agtggatctc tgctgtccct gtaataaacc
     4201 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     4261 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     4321 ttgtggatga atactgccat ttgtgaattc ggcgataccg tcgagatccg ttcactaatc
     4381 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     4441 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     4501 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     4561 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     4621 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     4681 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     4741 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     4801 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     4861 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     4921 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     4981 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     5041 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     5101 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     5161 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     5221 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     5281 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     5341 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     5401 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     5461 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     5521 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     5581 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     5641 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     5701 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     5761 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5821 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5881 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     5941 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct
     6001 ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac
     6061 tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct
     6121 aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg
     6181 ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac
     6241 catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg
     6301 aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttct ttgggagtga
     6361 attagccctt ccaactacta agtttgtagt acatatttaa caaatacaat ttctttaaaa
     6421 tgaaaataat tcagaggaat cacaggttta gagtaaatga aaccacaggt aattggcagt
     6481 ggtaataggg tatggggtgg gaagtttggg atgattttgg ttagcttgag ttatccagtt
     6541 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6601 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6661 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6721 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     6781 ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc
     6841 taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag
     6901 taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat
     6961 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
     7021 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
     7081 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
     7141 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
     7201 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac
     7261 aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag
     7321 agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct
     7381 tcacaaagat ccggggccca ctcataaatc cagttgccgc cacggtagcc aatcaccgta
     7441 tcgtataaat catcgtcggt acgttcggca tcgctcatca caatacgtgc ctggacgtcg
     7501 aggatttcgc gtgggtcaat gccgcgccag atccacatca gacggttaat catgcgatac
     7561 cagtgaggga tggttttacc atcaagggcc gactgcacag gcggttgtgc gccgtgatta
     7621 aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg
     7681 ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa ctattgtaac
     7741 ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag attagcgacc
     7801 ggagattggc gggacgaata cgacgcccat atcccacggc tgttcaatcc aggtatcttg
     7861 cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg cgaagatggt
     7921 gacaaagtgc gcttttggat acatttcacg aatcgcaacc gcagtaccac cggtatccac
     7981 caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca gcactttaag
     8041 ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat gacgaatacc
     8101 cagttcacgc gccagtaacg cacccggtac agaccgccac ggcttacggc aataatgcct
     8161 ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg
     8221 tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct
     8281 taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa
     8341 accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa
     8401 agtggtattc tcaaacatat ctcgcaagcc tgtcttgtgt ccaagctttt tgcaaaagcc
     8461 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     8521 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     8581 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     8641 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     8701 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     8761 acaccctaac tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga
     8821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10741 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    10801 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    10861 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    10921 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    10981 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
    11041 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
    11101 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
    11161 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
    11221 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
    11281 ggccacgatg cgtccggcgt agaggatctg gctagattac ttataatacc taatagattg
    11341 taaatgctaa ataaagttgt tatactgtgc tgtttttctg atttgtattt ttttattgtt
    11401 gtattgctat ttttaatttt ttcccaatat ttttgatcca cagttggttg gatttttgtt
    11461 gtggaacctg cagatgagga aggctgactt tatatgataa tatcaaactg gaaaaattga
    11521 tgttagaata aagatgtgtt tctgagatta aattagattt taataaaaat taccccagtc
    11581 tcaggttttc tatctgcaat gtgaaatgat ctcagagaag aataaatttt aaccatttac
    11641 attattaaat tgaaatcact aaaagtaaaa caaaggtgtg agactatata atagattgtt
    11701 ttccttattt cagagtcttt ggaacaaatt cttccaagat aaagaacttc gatcaatgat
    11761 tgaacaagat gtcaaaagaa cgtatgtaga actatttaca tgttactttc aataattcaa
    11821 attgttgtta tctctttcta attttaacaa aa
//
