LOCUS       sequence-461251-       10727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: p18sheLL
FEATURES             Location/Qualifiers
     source          1..10727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    255..376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(266..285)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     320..339
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(506..874)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     primer_bind     2804..2824
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2833..3261)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2943..2964)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3155..3174
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3307..3531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3520..3537
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    3574..4162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3680..3691
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4089..4109
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      7805..7814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7883..7907)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7917..7935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7938..7956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(7958..8161)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7987..8007)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8162..8541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8713..8732
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8807..8911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8912..8980,8981..9772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9130..9149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9943..10531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10432..10451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10685..10702
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
      241 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
      301 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      361 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
      421 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
      481 tggaatcgaa atctcgtagc acgtgtcagt cctgctcctc ggccacgaag tgcacgcagt
      541 tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca
      601 tggccggccc ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca
      661 gctcgtccag gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct
      721 ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca
      781 cgaagtcccg ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc
      841 gcgcggtgag caccggaacg gcactggtca acttggtact ttctttccac ataaatttgt
      901 agtcaatatg ttcaccccaa aaaagctgtt tgttaacttg ccaacctcat tctaaaatgt
      961 atatagaagc ccaaaagaca ataacaaaaa tattcttgta gaacaaaatg ggaaagaatg
     1021 ttccactaaa tatcaagatt tagagcaaag catgagatgt gtggggatag acagtgaggc
     1081 tgataaaata gagtagagct cagaaacaga cccattgata tatgtaagtg acctatgaaa
     1141 aaaatatggc attttacaat gggaaaatga tgatcttttt cttttttaga aaaacaggga
     1201 aatatattta tatgtaaaaa ataaaaggga acccatatgt cataccatac acacaaaaaa
     1261 attccagtga attataagtc taaatggaga aggcaaaact ttaaatcttt tagaaaataa
     1321 tatagaagca tgccatcatg acttcagtgt agagaaaaat ttcttatgac tcaaagtcct
     1381 aaccacaaag aaaagattgt taattagatt gcatgaatat taagacttat ttttaaaatt
     1441 aaaaaaccat taagaaaagt caggccatag aatgacagaa aatatttgca acaccccagt
     1501 aaagagaatt gtaatatgca gattataaaa agaagtctta caaatcagta aaaaataaaa
     1561 ctagacaaaa atttgaacag atgaaagaga aactctaaat aatcattaca catgagaaac
     1621 tcaatctcag aaatcagaga actatcattg catatacact aaattagaga aatattaaaa
     1681 ggctaagtaa catctgtggc aatattgatg gtatataacc ttgatatgat gtgatgagaa
     1741 cagtacttta ccccatgggc ttcctcccca aacccttacc ccagtataaa tcatgacaaa
     1801 tatactttaa aaaccattac cctatatcta accagtactc ctcaaaactg tcaaggtcat
     1861 caaaaataag aaaagtctga ggaactgtca aaactaagag gaacccaagg agacatgaga
     1921 attatatgta atgtggcatt ctgaatgaga tcccagaaca gaaaaagaac agtagctaaa
     1981 aaactaatga aatataaata aagtttgaac tttagttttt ttttaaaaaa gagtagcatt
     2041 aacacggcaa agccattttc atatttttct tgaacattaa gtacaagtct ataattaaaa
     2101 attttttaaa tgtagtctgg aacattgcca gaaacagaag tacagcagct atctgtgctg
     2161 tcgcctaact atccatagct gattggtcta aaatgagata catcaacgct cctccatgtt
     2221 ttttgttttc tttttaaatg aaaaacttta ttttttaaga ggagtttcag gttcatagca
     2281 aaattgagag gaaggtacat tcaagctgag gaagttttcc tctattccta gtttactgag
     2341 agattgcatc atgaatgggt gttaaatttt gtcaaatgct ttttctgtgt ctatcaatat
     2401 gaccatgtga ttttcttctt taacctgttg atgggacaaa ttacgttaat tgattttcaa
     2461 acgttgaacc acccttacat atctggaata aattctactt ggttgtggtg tatatttttt
     2521 gatacattct tggattcttt ttgctaatat tttgttgaaa atgtttgtat ctttgttcat
     2581 gagagatatt ggtctgttgt tttcttttct tgtaatgtca ttttctagtt ccggtattaa
     2641 ggtaatgctg gcctagttga atgatttagg aagtattccc tctgcttctg tcttctgaaa
     2701 gagattgtag aaagttgata caattttttt ttctttaaat atttgataga attctgcatg
     2761 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     2821 ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2881 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2941 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3001 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3061 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3121 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3181 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3241 atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct
     3301 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     3361 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     3421 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     3481 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     3541 atcagcgggt ttaaacgggc cgacggtatc gatgcgggga ggcggcccaa agggagatcc
     3601 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     3661 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca
     3721 gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca
     3781 ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg
     3841 cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac
     3901 aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc
     3961 cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca
     4021 aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag
     4081 cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa
     4141 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcctgc agcccggggg
     4201 atctcaagcg gcgacgaacc cgtcggcgaa gaagtatggc accctcttcc tcttcttggg
     4261 gatgaagtag tacaggggcc agaggtagta gtgggtgccg tggatgccga tgtattgggt
     4321 gctagcggga gcggttgggc tcacgattgg ccacacgctt gtggtgctgg gcagggtgat
     4381 gtcggggccg gtatacacgg ggacgtccca gctacttgtc aggggcacgg tcacgttgga
     4441 gtaactgcta gcgctgctga tggttgggct gtacttgaag aaggcgaagc ttgtggtgct
     4501 cctgctgggc acggggacgg cgggatccat atcgtcggcg tagatgtcga agagatcgtt
     4561 atcttcggta gcgctcacga ggggttggag ctcgatgtac tcggggctgg gggcgatggg
     4621 ggagatgtcg tgatagaaat gcacgcgggc gccgatctgg gtgccagacc ttgtgaacat
     4681 ggtggctctc tggcccagcc gggagaacct cacggtgccc ctgcggctgg tcagggcggg
     4741 gcggtgcaac ctaatgatgt ccatgaagtc gctgtcgggc acgtcggacc tggggtcgaa
     4801 ggtcagggtg gtatcgacgg gttcgaaagc ggggttatcg taggtgatca ggctgctggg
     4861 cctggtcagg aattcgggat tggcgacgct gacttgctga taagcgcggg aatacaacct
     4921 tgggccggcc acccttctga cggtggggag gggtgtggag gagattggct cttcgccggt
     4981 gccgctgctg gcgaaggtct gcagggggat ctcttcgtag ccgtgggtgc cgctggtggg
     5041 tgtgcccacg aacacgttgc cggcgacttc gccggtctgg ggcacctcga tgatgctggg
     5101 gtcgctgaag gcggggtttg tgaagtttgt ggtactgatg ctgacgcttg tgctgctggg
     5161 ggtaatgtcg agcacggcgg gggtggtggt gccggcgctg gtgatgtcga agccgctggt
     5221 cccggtgaag gtggggcggg gggcgccgct ggtcacgacg gagctatctt cgatcagggt
     5281 cacgatgctg ggatcggtag gcccgacggg ctcgatcacg acgggaggcc tggtggggcc
     5341 cacgtcgacg acggtgttgc tcctgccccc gagggggata tagccggtcc tgccgccggt
     5401 gccggacccg gtgccgatcc ccaagccgcc gaggaagatg cccaaagagc tccactggag
     5461 gatcttgtcg gccagggttg tcccttcgac tttgggcacc acgtcggggg ggcaggtgcc
     5521 gctctgcttg caggtcttgt acagatcggt cacgctggct ctcttcctcc tggcagccct
     5581 atggctgacc atgcggccgc aatccaattc gctttatgat aacaatctgt gattgtcacc
     5641 ataagcagcc acaataaaat aaaaggaaac acggacaccc aaagtagtcg gttccgccac
     5701 ggacttgcgc gttacgacag gccaatcact ggtttgtgac cacctgctcc gaggttggga
     5761 ttagccgcat tcaggggccg gaggattctt atgtagctca ataggctctt cacaccttgt
     5821 tcacaactag cgtcccatgg cgttagccat aggtaggccg ccaacgcagc ctggaccacc
     5881 gtcaccggtg agggatgtcc agactcatca gcctaagcta cactctgggg ttgagtgctg
     5941 agcgcaacgc atcgaagatt ccgaggtggt actgggcttc tcgaagtaca taagcggata
     6001 acggatccgt cgctttcaac cacgcaagca gtctatacga catcaccggg gaaacagaag
     6061 tgcttgttcg tggtggtact ggtttgtacc cccttctatt gaacttggtt ttgtgcgtct
     6121 aagttacggg aagggagtat aaaacaggcg tacaagggta ccgcaatacc ggagtactag
     6181 ccgccacgtg ggcctctggg gtgggtacaa ccccagagct gttttaagct tctgcaggtc
     6241 gacccatcga attcctgcag cccgggggat ccactagttc tagctcattt gcgtgccctc
     6301 actctgaccc tttttgcggg cttgctgctg gtggttgcgc tgggggcgct cctcttcctg
     6361 ggcccgattg ttggttttct cctgaggccg gcttgcacga ggaacttcct gcccaatggg
     6421 tactggtcca gatccaggct gaatttctct ttcaggtcga cgttccagaa tttcagtttg
     6481 tcgtatgggt ctttgttctc ggcgggggcg gcgtctttct ggcatgtgat ggccacgctc
     6541 tgcacgaatc tgtaggtgtc gacgagggag gtggtagggg gtggtggcac gccgaaattc
     6601 cagtcttcca ggatggagga gttcatggag tggatgtagc tcatcacgtc ggcggtgagg
     6661 gtgatggtgc agagttggaa gatgaattgg aggtcgtact cttccacgtg ccgagagtat
     6721 tgtttgaact ttgtggcgtc gtactggccg ggcacggggc tttgggtgct ggcgcagatg
     6781 gtcaggtttg tggacctggt tgtgtccacg acggtcacga acagctggtt gtgccaacac
     6841 accccgttat tgtggccttg ggctttgtgg agccagtagg gcttgttgaa gagttggcta
     6901 tcgcttgtga cgatgctccc ggaggggctt gggctgtaga cgcaggaccc ggggctggcc
     6961 ctcattccgg tccccttgat gtacaggctc tgggggactg tatctcccat tgtgccggcc
     7021 cggttccaga agtggcgggc gaacaattgt tcccttctga gacagaagaa catgctgtcg
     7081 ccgtaggggt cggcgctcat ctggaggtag tcggggtact tgcagatgct ttggcagatg
     7141 tcgaggggca cttcgcactt ggtgtcctgg agggtggaga aatccatagc gccgtagccg
     7201 gtgtccacca tgtcgccgtc ctcgagcacg gtattcttca actccagggg tggacagtcc
     7261 ccttggctca ggggcctgct cttgcaggcg gtccccttgg cccaatgctc gccgatggcg
     7321 ggtgcgcatc ccaggatgca cagttgggtt tgtttgtagt ccacgctgac gttatcgcgc
     7381 acatcttcgg agacgttgct ggttgctgcg tggctggact cggtatcgtc cagcttgttg
     7441 tagaaggggt ggccggacaa ccccacgccc aggggttggc ccctcccgat ctcgacgccg
     7501 gcgcaagccc agaccagcct ctgggtctcg gggttgtaga tggaggtgtc gggcaggccg
     7561 aacttgttgg gatcgggcag ttggacgcgg aacacccggt actgataggc gctgactttg
     7621 gggatgtctt gtttgtttcc gccgccggcg ggcacgcgga agtaggggtt gcccacggtc
     7681 agcagccggc tggacccggc gtggtagaag attgaggttc tggtgacgta gtcgtctgtg
     7741 ttcaccaccc gggcgacgct gggtgggggc aagtacactg tgttatcgga tggtctccag
     7801 agggccatgg tggcgaccgg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac
     7861 agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctcgt cgacgatctc
     7921 tatcactgat agggagatct ctatcactga tagggagagc tctgcttata tagacctccc
     7981 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     8041 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     8101 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     8161 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     8221 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     8281 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     8341 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     8401 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     8461 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     8521 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     8581 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     8641 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     8701 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     8761 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     9061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     9121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    10021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    10081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    10141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    10201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10621 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10681 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgca
//
