LOCUS       sequence-461259-       18264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bigMamAct assembled plasmid for 6colors cellular labelling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18264)
  AUTHORS   Pelosse M, Marcia M
  TITLE     biGMamAct: efficient CRISPR/Cas9-mediated docking of large 
            functional DNA cargoes at the ACTB locus
  JOURNAL   Synthetic Biology 2025.02.13
REFERENCE   2  (bases 1 to 18264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology 2025.02.13"
COMMENT     Sequence Label: pDONOR 6colors
FEATURES             Location/Qualifiers
     source          1..18264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      564..573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             570..1247
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             570..1247
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             1272..1292
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1296..1316
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1320..1340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1350..1403
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1410..2435
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1410..2435
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    2483..2564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2704..2722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2778..2796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2815..2833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2852..2870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2889..2907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2926..2944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2963..2981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3175..3249
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3265..3972
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3407..3425)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    4162..4296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4484..4502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4521..4539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4558..4576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4595..4613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4632..4650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4669..4687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4706..4724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4743..4761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      4965..4974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4971..4973,4974..4976,4977..5687)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5016..5037)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5277..5296)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5624..5645
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7262..7396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7584..7602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7621..7639
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7658..7676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7695..7713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7732..7750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7769..7787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7806..7824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7843..7861
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             8049..8426
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      8439..8448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9588..9722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    9910..9928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9947..9965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9984..10002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10021..10039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10058..10076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10095..10113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10132..10150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10169..10187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             10642..11337
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    11536..11670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    11858..11876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11895..11913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11932..11950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11969..11987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12006..12024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12043..12061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12080..12098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12117..12135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      12322..12331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12328..13035
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     polyA_signal    14371..14505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      14873..15461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15362..15381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15521..16054)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(16224..16229,16230..16246,16247..16252))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(16280..16313)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    16384..16518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(16567..17166)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     17147..17166
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(17170..17226)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(17227..17232,17233..17268,17269..17271,
                     17272..17307,17308..17310,17311..17346,17347..17352,
                     17353..17970))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(17971..18024)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gctctggcta actagagaac ccactgctta ctggcttatc
      541 gaaatataac cgtcagatcc gctgccacca tggtgagcgt gatcaagccc gagatgaaga
      601 tcaagctgtg catgaggggc accgtgaacg gccacaactt cgtgatcgag ggcgagggca
      661 agggcaaccc ctacgagggc acccagatcc tggacctgaa cgtgaccgag ggcgcccccc
      721 tgcccttcgc ctacgacatc ctgaccaccg tgttccagta cggcaacagg gccttcacca
      781 agtaccccgc cgacatccag gactacttca agcagacctt ccccgagggc taccactggg
      841 agaggagcat gacctacgag gaccagggca tctgcaccgc caccagcaac atcagcatga
      901 ggggcgactg cttcttctac gacatcaggt tcgacggcac caacttcccc cccaacggcc
      961 ccgtgatgca gaagaagacc ctgaagtggg agcccagcac cgagaagatg tacgtggagg
     1021 acggcgtgct gaagggcgac gtgaacatga ggctgctgct ggagggcggc ggccactaca
     1081 ggtgcgactt caagaccacc tacaaggcca agaaggaggt gaggctgccc gacgcccaca
     1141 agatcgacca caggatcgag atcctgaagc acgacaagga ctacaacaag gtgaagctgt
     1201 acgagaacgc cgtggccagg tactccatgc tgcccagcca ggccaagtcc ggactcagat
     1261 ctcgagctga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc
     1321 caaaaaagaa gagaaaggta ggatccaccg agggcagagg aagtctgcta acatgcggtg
     1381 acgtcgagga gaatcctggc cccgtataca tgaaaaagcc tgaactcacc gcgacgtctg
     1441 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     1501 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     1561 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     1621 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     1681 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     1741 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     1801 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     1861 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     1921 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     1981 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     2041 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     2101 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     2161 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     2221 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     2281 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     2341 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     2401 gacgccccag cactcgtccg agggcaaagg aatagcacct ccccctgaac ctgaaacaaa
     2461 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2521 caatagcatc acaaatttca caaataaagc atttttttca ctgctgatca ctgcttgagc
     2581 ctagaagagt tcggtcccag ggctggcact ctgtcaagtg aacgctctat ggtctaaaga
     2641 tttaaatcga cctactccgg aatattaata gatcactcga gggatccaag ctggccgcac
     2701 gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa
     2761 ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta
     2821 tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg
     2881 tctcgagctc cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata
     2941 gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacccc ctatataagc
     3001 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     3061 catagaagac accgggaccg atccagcctc cgcggtggcg gccatcacaa gtcataccgt
     3121 cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa ttcaaagggc tagcatgtcc
     3181 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
     3241 cgcgccaaga tccattcgtt gggggtgagc aagggcgagg aggataacat ggccatcatc
     3301 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3361 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3421 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3481 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3541 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3601 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3661 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3721 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3781 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3841 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3901 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3961 ctgtacaagt aacctacgtc gacgagctca ctagtcgcgg ccgctttcga atctagagcc
     4021 tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     4081 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4141 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     4201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4261 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
     4321 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     4381 actatcataa ccatttaaat aaactggata ctattgcacg tttaaatcga cctactccgg
     4441 aatattaata gatcactcga gggatccaag ctggccgcac gtctccctat cagtgataga
     4501 gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc
     4561 ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacg
     4621 tctagaacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc cctatcagtg
     4681 atagagaagg tacgtctaga acgtctccct atcagtgata gagaagtcga cacgtctcga
     4741 gctccctatc agtgatagag aaggtacccc ctatataagc agagctcgtt tagtgaaccg
     4801 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     4861 atccagcctc cgcggtggcg gccatcacaa gtcataccgt cccaccatcg ggcgcggatc
     4921 ccggtccgaa gcgcgcggaa ttcaaagggc tagcgctacc ggtcgccacc atggtgagca
     4981 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5041 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5101 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5161 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     5221 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5281 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5341 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5401 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5461 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5521 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     5581 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5641 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     5701 ctcgagtgcg tgagtgcatc tccatccacg ttggccaggc tggtgtccag attggcaatg
     5761 cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag atgccaagtg
     5821 acaagaccat tgggggagga gatgactcct tcaacacctt cttcagtgag acgggcgctg
     5881 gcaagcacgt gccccgggct gtgtttgtag acttggaacc cacagtcatt gatgaagttc
     5941 gcactggcac ctaccgccag ctcttccacc ctgagcagct catcacaggc aaggaagatg
     6001 ctgccaataa ctatgcccga gggcactaca ccattggcaa ggagatcatt gaccttgtgt
     6061 tggaccgaat tcgcaagctg gctgaccagt gcaccggtct tcagggcttc ttggttttcc
     6121 acagctttgg tgggggaact ggttctgggt taacctccct gctcatggaa cgtctctcag
     6181 ttgattatgg caagaagtcc aagctggagt tctccattta cccagcaccc caggtttcca
     6241 cagctgtagt tgagccctac aactccatcc tcaccaccca caccaccctg gagcactctg
     6301 attgtgcctt catggtagac aatgaggcca tctatgacat ctgtcgtaga aacctcgata
     6361 tcgagcgccc aacctacact aaccttaacc gccttattag ccagattgtg tcctccatca
     6421 ctgcttccct gagatttgat ggagccctga atgttgacct gacagaattc cagaccaacc
     6481 tggtgcccta cccccgcatc cacttccctc tggccacata tgcccctgtc atctctgctg
     6541 agaaagccta ccatgaacag ctttctgtag cagagatcac caatgcttgc tttgagccag
     6601 ccaaccagat ggtgaaatgt gaccctcgcc atggtaaata catggcttgc tgcctgttgt
     6661 accgtggtga cgtggttccc aaagatgtca atgctgccat tgccaccatc aaaaccaagc
     6721 gcagcatcca gtttgtggat tggtgcccca ctggcttcaa ggttggcatc aactaccagc
     6781 ctcccactgt ggtgcctggt ggagacctgg ccaaggtaca gagagctgtg tgcatgctga
     6841 gcaacaccac agccattgct gaggcctggg ctcgcctgga ccacaagttt gacctgatgt
     6901 atgccaagcg tgcctttgtt cactggtacg tgggtgaggg gatggaggaa ggcgagtttt
     6961 cagaggcccg tgaagatatg gctgcccttg agaaggatta tgaggaggtt ggtgtggatt
     7021 ctgttgaagg agagggtgag gaagaaggag aggaatacta aggatccacc ggcctacgtc
     7081 gacgagctca ctagtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt
     7141 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7261 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7381 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
     7441 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa ccatttaaat
     7501 aaacctaatg atgcctgatg tttaaatcga cctactccgg aatattaata gatcactcga
     7561 gggatccaag ctggccgcac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
     7621 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
     7681 gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca
     7741 gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg tacgtctaga
     7801 acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag
     7861 aaggtacccc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     7921 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggtggcg
     7981 gccatcacaa gtcataccgt cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa
     8041 ttcaaaggat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg
     8101 cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct
     8161 attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca
     8221 aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg
     8281 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg
     8341 ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg
     8401 ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggcc gaagggagcg
     8461 tggcaagaca gcccgatctg ctgacatgcg acgacgaacc aatccacatt ccaggagcta
     8521 tccagcctca tggactgctg ctggctctgg ccgctgacat gactatcgtg gcaggctctg
     8581 ataacctgcc agagctgacc gggctggcta tcggagcact gattggccgg tccgcagccg
     8641 acgtgttcga ttctgagact cacaatagac tgaccatcgc cctggctgaa cctggggctg
     8701 cagtgggagc cccaattacc gtcggcttca caatgcgaaa ggacgctggc tttatcgggt
     8761 cctggcaccg ccatgatcag ctgatttttc tggagctgga accacctcag agagacgtgg
     8821 cagagccaca ggctttcttt cggagaacaa acagcgccat caggcgcctg caggccgctg
     8881 agactctgga atccgcttgc gcagccgctg cacaggaagt gaggaagatc accgggttcg
     8941 atcgcgtcat gatctacaga ttcgcctcag actttagcgg agaagtgatc gcagaagatc
     9001 gctgtgccga ggtcgaaagt aaactgggcc tgcattaccc agcctcaacc gtgcctgcac
     9061 aggcacgacg gctgtataca atcaaccccg tgcggatcat tcccgacatt aattacagac
     9121 cagtgcccgt cacacctgac ctgaacccag tgactgggag gcccatcgat ctgagcttcg
     9181 ccattctgcg cagcgtgtcc cctgtccacc tggagtttat gaggaatatc ggaatgcatg
     9241 gcaccatgtc tatcagtatt ctgagaggcg aaaggctgtg ggggctgatc gtgtgccacc
     9301 atcggacacc ttactatgtg gacctggacg gcaggcaggc ttgtgaactg gtggctcagg
     9361 tgctggcttg gcagattggc gtgatgaagg aggaataacc tacgtcgacg agctcactag
     9421 tcgcggccgc tttcgaatct agagcctgca gtctcgacaa gcttgtcgag aagtactaga
     9481 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9541 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     9601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9721 tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag
     9781 ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaac ggttcacata
     9841 gcttagttta aatcgaccta ctccggaata ttaatagatc actcgaggga tccaagctgg
     9901 ccgcacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
     9961 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
    10021 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
    10081 gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca
    10141 gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat
    10201 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
    10261 gacctccata gaagacaccg ggaccgatcc agcctccgcg gtggcggcca tcacaagtca
    10321 taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggatgagg
    10381 cttcgtgaac ccttgttgtc aggctctgct gccatgcccg gtgccagcct gcaaagggca
    10441 tgtcgtcttc tggttgcggt gtgtgcattg catctcgggg tgactcttgt gtattatctt
    10501 gcgggaagag atctctcccg gcttccacag ttggtgggcg tgagtacccc cttgcaaggc
    10561 ggttcaaata gcgctgcagc tattggacaa agcagcggcg aactgaggac aggcggcgct
    10621 aaagacccac cagtagcgac tagcgagctg attaaggaga acatgcacat gaagctgtac
    10681 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
    10741 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
    10801 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
    10861 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
    10921 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
    10981 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
    11041 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
    11101 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
    11161 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
    11221 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
    11281 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgga
    11341 tcctagccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt
    11401 ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
    11461 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    11521 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    11581 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11641 ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc
    11701 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc
    11761 ataaccattt aaataaacac tgacattgac ttggtttaaa tcgacctact ccggaatatt
    11821 aatagatcac tcgagggatc caagctggcc gcacgtctcc ctatcagtga tagagaagtc
    11881 gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca
    11941 gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg tacgtctaga
    12001 acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag
    12061 aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc
    12121 tatcagtgat agagaaggta ccccctatat aagcagagct cgtttagtga accgtcagat
    12181 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
    12241 cctccgcggt ggcggccatc acaagtcata ccgtcccacc atcgggcgcg gatcccggtc
    12301 cgaagcgcgc ggaattcaaa ggccgccatg gtgagcaagg gcgaggagac cacaatgggc
    12361 gtaatcaagc ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa tggccacgcc
    12421 ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg gcaccaacac catcaacctg
    12481 gaggtgaagg agggagcccc cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc
    12541 tacggcaaca gggccttcac caagtacccc gacgacatcc ccaactactt caagcagtcc
    12601 ttccccgagg gctactcttg ggagcgcacc atgaccttcg aggacaaggg catcgtgaag
    12661 gtgaagtccg acatctccat ggaggaggac tccttcatct acgagataca cctcaagggc
    12721 gagaacttcc cccccaacgg ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc
    12781 accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca caagctgctg
    12841 ctggagggcg gcggccacca ccgcgttgac ttcaagacca tctacagggc caagaaggcg
    12901 gtgaagctgc ccgactatca ctttgtggac caccgcatcg agatcctgaa ccacgacaag
    12961 gactacaaca aggtgaccgt ttacgagagc gccgtggccc gcaactccac cgacggcatg
    13021 gacgagctgt acaagggatc aggatcagga tcaatggatg atgatatcgc cgcgctcgtc
    13081 gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg gcgacgatgc cccccgggcc
    13141 gtcttcccct ccatcgtggg gcgccccagg caccagggcg tgatggtggg catgggtcag
    13201 aaggattcct atgtgggcga cgaggcccag agcaagagag gcatcctcac cctgaagtac
    13261 cccatcgagc acggcatcgt caccaactgg gacgacatgg agaaaatctg gcaccacacc
    13321 ttctacaatg agctgcgtgt ggctcccgag gagcaccccg tgctgctgac cgaggccccc
    13381 ctgaacccca aggccaaccg cgagaagatg acccagatca tgtttgagac cttcaacacc
    13441 ccagccatgt acgttgctat ccaggctgtg ctatccctgt acgcctctgg ccgtaccact
    13501 ggcatcgtga tggactccgg tgacggggtc acccacactg tgcccatcta cgaggggtat
    13561 gccctccccc atgccatcct gcgtctggac ctggctggcc gggacctgac tgactacctc
    13621 atgaagatcc tcaccgagcg cggctacagc ttcaccacca cggccgagcg ggaaatcgtg
    13681 cgtgacatta aggagaagct gtgctacgtc gccctggact tcgagcaaga gatggccacg
    13741 gctgcttcca gctcctccct ggagaagagc tacgagctgc ctgacggcca ggtcatcacc
    13801 attggcaatg agcggttccg ctgccctgag gcactcttcc agccttcctt cctgggcatg
    13861 gagtcctgtg gcatccacga aactaccttc aactccatca tgaagtgtga cgtggacatc
    13921 cgcaaagacc tgtacgccaa cacagtgctg tctggcggca ccaccatgta ccctggcatt
    13981 gccgacagga tgcagaagga gatcactgcc ctggcaccca gcacaatgaa gatcaagatc
    14041 attgctcctc ctgagcgcaa gtactccgtg tggatcggcg gctccatcct ggcctcgctg
    14101 tccaccttcc agcagatgtg gatcagcaag caggagtatg acgagtccgg cccctccatc
    14161 gtccaccgca aatgcttctg acctacgtcg acgagctcac tagtcgcggc cgctttcgaa
    14221 tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat
    14281 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    14341 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    14401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    14461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
    14521 cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
    14581 tgagcaataa ctatcataac catttaaata aatctatatc tcaatcgggg ttacgtcgat
    14641 accccaccga gaccccattg ggaccaatac gcccgcgttt cttccttttc cccaccccaa
    14701 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aagccctgcc
    14761 atagccacta cgggttaatt aaaaactata tctcaatcgg ggtttaaaca ggagatccgg
    14821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    14881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    14941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    15001 cgcagatacc aaatactatt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    15061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    15121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    15181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    15241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    15301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    15361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    15421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    15481 ttttacggtt cctggccccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
    15541 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
    15601 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
    15661 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
    15721 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
    15781 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
    15841 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
    15901 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
    15961 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
    16021 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
    16081 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
    16141 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
    16201 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
    16261 gtgccttcgc cgcggtacca taacttcgta tagcatacat tatacgaagt tatctgggtt
    16321 taaacgctct atggtctaaa gtttttatcg aggctaactg aaacacggaa caagcagtga
    16381 tcagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    16441 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    16501 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
    16561 gaggtgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
    16621 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgt ggggcgcgga
    16681 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
    16741 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
    16801 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
    16861 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
    16921 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
    16981 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
    17041 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
    17101 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
    17161 ggtcatccca ggaccggggt tttcttccac gtctcctgct tgctttaaca gagagaagtt
    17221 cgtggccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc
    17281 aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat cgtcaagggc
    17341 gtcggccggc ccgccgcttt cgcactttag ctgtttctcc aggccacata tgattagttc
    17401 caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa ttgcttgtct
    17461 cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg ctacttgatg
    17521 ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca gagcgtacag
    17581 tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt ccagggtttc
    17641 gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc gatgtgagag
    17701 gagagcacag cggaatgact tggcgttgtt ccgcagaaag tcttgccatg actcgccttc
    17761 cagggggcag aagtgggtat gatgcctgtc cagcatctcg attggcaggg catcgagcag
    17821 ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca gcttttgagc
    17881 gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca gagcgccgtt
    17941 tatgactttg ctcttgtcca gtctagacat ggggccagga ttctcctcga cgtcaccgca
    18001 tgttagcaga cttcctctgc cctctcccga tccggccccg aagcatttgc ggtggacgat
    18061 ggaggggccg gactcgtcat actcctgctt gctgatccac atctgctgga aggtggacag
    18121 cgaggccagg atggagccgc cgatccacac ggagtacttg cgctcaggag gagcaatgat
    18181 ctgaggaggg aaggggacag gcagtgagga ccctggatgt gacagctccc cacacaccct
    18241 gtggtgtgtg gggagctgtc tcat
//
