LOCUS       sequence-461275-        9611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Dou H, Huang C, Singh M, Carpenter PB, Yeh ET
  TITLE     Regulation of DNA repair through deSUMOylation and SUMOylation of 
            replication protein A complex.
  JOURNAL   Mol Cell. 2010 Aug 13. 39(3):333-45.
  PUBMED    20705237
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Aug 13. 39(3):333-45."
COMMENT     Sequence Label: 3xFLAG-SENP6
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     172..191
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    954..1088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(991..1010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1045..1064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1093..1112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1094..1112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1126..1142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1126..1142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1139..1161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1150..1166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1174..1180,1181..1198,1199..1204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1219..1240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1357..1374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1528..2116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1608..1627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2287..3078,3079..3147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2910..2929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3148..3252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3279..3734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3366..3385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3576..3597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3860..3882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3874..3891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3875..3891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4052..4431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4432..4635
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4586..4606
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4665..4730
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    8104..8726
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8303..8322)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(8750..8770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8768..9065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             join(9119..9611,1..302)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9173..9192)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
       61 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
      121 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
      181 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
      241 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
      301 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
      361 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
      421 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccgggct
      481 cgatcccctc gcgagttggt tcagctgctg cctgaggctg gacgacctcg cggagttcta
      541 ccggcagtgc aaatccgtcg gcatccagga aaccagcagc ggctatccgc gcatccatgc
      601 ccccgaactg caggagtggg gaggcacgat ggccgctttg gtcgacccgg acgggacgct
      661 cctgcgcctg atacagaacg aattgcttgc aggcatctca tgagtgtgtc ttcccgtttt
      721 ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg cgagctgctc
      781 accacccact cgccaagctg gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      841 ctccgccgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      901 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1081 tctggatcaa ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc
     1141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3241 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     3301 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3361 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3421 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3481 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3541 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3601 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3661 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3721 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3841 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
     3901 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     3961 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     4021 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     4081 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4141 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4201 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     4261 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     4321 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     4381 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     4441 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4501 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4561 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4621 tatataagca gagctcgttt agtgaaccgt cagaattaac catggactac aaagaccatg
     4681 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag cttgcggccg
     4741 cgaattcatc gatagatctg atatcggtac caatggcggc cggcaagagc ggcggtagcg
     4801 caggggagat tacttttctg gaagctttgg ctagatcaga gtctaagaga gatggaggtt
     4861 ttaaaaataa ttggagcttt gatcatgaag aagaaagtga aggagataca gataaagatg
     4921 ggacaaatct gctcagtgtg gatgaagatg aggattctga aacctcaaaa ggaaaaaagt
     4981 taaatcgtcg atctgaaatt gttgctaata gctctggtga attcatcttg aagacatatg
     5041 taagacgaaa caagtctgaa agttttaaaa ctttgaaagg caacccaatt ggacttaaca
     5101 tgttgagcaa caataagaaa ttgagtgaaa atacgcaaaa tacgtcatta tgttctggaa
     5161 ctgtagttca tggtagacgt tttcatcatg ctcatgcaca gataccagta gtaaaaacag
     5221 cagcccaaag gaaagaatac ccacctcatg tccaaaaagt tgaaattaat cctgtaaggt
     5281 taagtcggct ccaaggtgtt gaacgtataa tgaagaaaac agaagagtcc gaatcacaag
     5341 tggagcctga aattaagagg aaagtacaac agaaacggca ctgtagtacc tatcagccta
     5401 ctcctcctct atctcctgct tcaaaaaaat gtttaaccca tttagaggat ttgcaaagga
     5461 attgcagaca agctattact ttgaatgagt ctactggacc attattaaga acgtcaattc
     5521 atcagaattc tggaggacag aagtcacaaa acacaggatt aacaaccaag aagttttatg
     5581 gcaacaatgt ggaaaaggtt ccaattgata ttattgtgaa ttgtgatgac agtaaacaca
     5641 cttatttaca gactaatgga aaagtcattt tacctggggc aaaaataccc aaaatcacaa
     5701 acttgaaaga aaggaaaaca agtttgtcag acctaaatga tccaatcatt ttgtccagtg
     5761 atgatgatga tgacaacgac agaactaaca gaagagaaag catatctcct cagcctgctg
     5821 attcagcatg ttcttcccct gcaccatcca ctggaaaagt agaagcagcg ctaaatgaaa
     5881 atacttgcag agcagagcgt gaactacgaa gcattccaga agactcagag ttaaatacag
     5941 ttacattacc aagaaaagca agaatgaaag accagtttgg caattctatt atcaacacac
     6001 ctctgaaacg tcgtaaagtg ttttctcaag aacctccaga tgctttagct ttaagctgcc
     6061 aaagttcctt tgacagtgtc attttaaact gtcgaagtat acgagtagga acgctcttcc
     6121 ggctgttaat agagcctgta attttttgtt tagattttat caagatacag ctagacgaac
     6181 cagaccatga tcctgtagag attatattaa atacctctga tctaactaaa tgtgaatggt
     6241 gtaatgtccg aaaattacct gtagtgtttc ttcaagcaat tccagcagtt tatcaaaagc
     6301 tgagcatcca actgcaaatg aataaggagg ataaagtttg gaatgattgt aaaggagtaa
     6361 ataaattaac aaatttagaa gaacaatata taattttaat ttttcaaaat ggccttgatc
     6421 ctccggcaaa tatggtattt gaaagtatca ttaatgaaat tggtataaag aataacatct
     6481 ccaatttttt tgcgaaaatt ccctttgaag gagctaatgg cagacttgtt gcctgtacaa
     6541 gaacctatga agagagcatc aaaggaagtt gtgggcaaaa ggaaaacaaa attaaaactg
     6601 tatcatttga atctaaaata caacttagaa gcaaacaaga atttcagttt tttgatgaag
     6661 aagaagaaac tggagaaaac cacaccatct tcattggccc agtagaaaag ttgatagtat
     6721 atccaccacc tccagctaag ggaggcatct ctgttaccaa tgaggacctg cactgtctaa
     6781 atgaaggaga atttttaaat gatgttatta tagactttta tttgaaatac ttggtgcttg
     6841 aaaaactgaa gaaggaagac gctgaccgaa ttcatatatt cagttctttt ttctataaac
     6901 gccttaatca gagagagagg agaaatcatg aaacaactaa tctgtcaata cagcaaaaac
     6961 ggcatgggag agtaaaaaca tggacccggc acgtagatat ttttgagaag gattttattt
     7021 ttgtacccct taatgaagct gcacactggt ttttggctgt tgtttgtttc cccggtttgg
     7081 aaaaaccaaa gtatgaacct aatcctcatt accatgaaaa tgctgtcata cagaaatgtt
     7141 caactgtaga ggacagttgt atttcttctt cagccagtga aatggagagt tgttcacaaa
     7201 actcttctgc caagcctgta attaagaaga tgctaaacaa aaaacattgc atagctgtaa
     7261 ttgattccaa tcctgggcag gaagaaagtg accctcgtta taagagaaac atatgcagtg
     7321 taaaatacag tgtgaaaaaa ataaatcata ctgcgagtga aaatgaagaa ttcaataaag
     7381 gagaatctac atcccagaaa gttgctgata ggactaaaag tgagaatggc ctacagaatg
     7441 aaagtttaag ttccacacat catacagatg gcttaagcaa aatcagacta aactatagcg
     7501 atgaatcacc tgaagctggt aaaatgcttg aagatgaact cgtcgacttc tcagaaaatc
     7561 aggataacca ggatgatagc agtgacgatg gattcctcgc tgatgacaac tgcagttcag
     7621 aaataggaca gtggcattta aagcctacta tctgtgaaca accttgtatc ctacttatgg
     7681 actcactccg aggcccttct cggtcaaatg ttgtcaaaat tttaagagag tatttagaag
     7741 tggaatggga agttaaaaaa ggaagcaaaa gaagtttttc caaagatgtt atgaagggct
     7801 ctaatccaaa agtaccacag caaaacaact tcagtgactg tggtgtatat gtattgcagt
     7861 atgtagagag cttttttgag aatccaattc tcagttttga actacctatg aatttggcaa
     7921 actggtttcc tccaccaaga atgagaacaa aaagagaaga aatccgaaac ataattctga
     7981 agctacagga agatcagagc aaagagaaaa gaaagcataa ggacacttac tcaacagaag
     8041 cacctttagg cgaaggaaca gaacaatatg tcaatagtat ctcagattga tctagaggat
     8101 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     8161 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     8221 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     8281 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     8341 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     8401 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     8461 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     8521 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     8581 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     8641 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     8701 tccactcagt agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt
     8761 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8821 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8881 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9001 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9061 tttggaggaa ttgatcagct tgggatctga tcaagagaca ggatgaggat cgtttcgcat
     9121 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     9181 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     9241 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     9301 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     9361 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     9421 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     9481 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     9541 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     9601 gcatcagggg c
//
