LOCUS       sequence-46131-e       18321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 18321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pBT272_(pRosa26-GTET)
FEATURES             Location/Qualifiers
     source          1..18321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1183..1486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1488..1765
                     /label=chicken beta-actin promoter
     intron          1767..2784
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2708..2730
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2792..2811
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2871..2880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2922..2943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    3170..3203
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3204..3237)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3238..3271)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3280..3313
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3320..3353)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3381..3485
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3487..3844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3487..3678
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3510..3530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3695..3830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3879..4673
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3879..4673
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3933..3952)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4543..4562
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4861..4880)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4905..4952
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(5144..5177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5297..5911,5912..5917,5918..5953,5954..5956,5957..5992,
                     5993..5995,5996..6031,6032..6040)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    6162..6283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6253..6272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    join(6807..6825,6826..6848,6849..6867,6868..6890,6891..6909,
                     6910..6932,6933..6951,6952..6974,6975..6993,6994..7016,
                     7017..7035,7036..7058,7059..7077)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        7110..7148
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7145..7169
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7273..7282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7279..8706
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             8716..8745
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8752..8781
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8788..8817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    8955..9036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9555..13825
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        13874..14367
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     14300..14319
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          15042..15107
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(15175..15192)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15181..15405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(15473..15493)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15473..15491)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15512..15528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15512..15528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15525..15547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15536..15552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15560..15566,15567..15584,15585..15590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15605..15626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15743..15760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15914..16502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15994..16013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16673..17464,17465..17533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17296..17315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17534..17638)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(17664..18119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17801..17822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     18013..18032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     18246..18268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18260..18277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18261..18277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18287..18306
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18287..18305
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagcct taattaaccg tttaaacaat tctgcaggaa tctagttatt aatagtaatc
     1141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1501 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1561 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg
     1621 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     1681 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     1741 taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg ccccgtgccc
     1801 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1861 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1921 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1981 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     2041 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2101 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     2161 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     2221 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2281 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     2341 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     2401 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     2461 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     2521 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2581 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2641 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2701 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     2761 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     2821 cattttggca aagaattgat ttgataccgc gggcccggga tccgatatcc gccaccatgg
     2881 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2941 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3001 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3061 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3121 acgacttctt caagtccgcc atgcccgaag gttggtatca aagatctccg aagttcctat
     3181 tctctagaaa gtataggaac ttcataactt cgtatagtac acattatacg aagttatata
     3241 acttcgtata ggatacttta tacgaagtta tggatctccg aagttcctat tctctagaaa
     3301 gtataggaac ttcggatcta taacttcgta tagcatacat tatacgaagt tattcaggtg
     3361 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3481 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3661 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3841 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     3901 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3961 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4021 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4081 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4141 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4201 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4261 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4321 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4381 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     4441 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4501 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4561 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4621 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4681 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4741 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4801 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     4861 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4921 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     4981 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     5041 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     5101 ggcaggccct gccatagcct caggttactc aggatctgga tctataactt cgtatagcat
     5161 acattatacg aagttatggt tacaagacag gtttaaggag accaatagaa actgggcatg
     5221 tggagacaga gaagactctt gggtttctga taggcactga ctctctctgc ctattggtct
     5281 attttcccac ccttagtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     5341 actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5401 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc
     5461 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5521 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
     5581 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5641 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5701 cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga
     5761 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     5821 gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     5881 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
     5941 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6001 cgctcttgac gattttgacc ttgacatgct ccccggatga gaattctaga taactgatca
     6061 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6121 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatttaaat ggggacagcc
     6301 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     6361 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     6421 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     6481 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgagtgcac
     6541 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     6601 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     6661 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     6721 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     6781 ctgaagatct gattcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6841 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     6901 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     6961 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     7021 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     7081 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     7141 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     7201 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgag ctcggtaccc
     7261 ggggatcggg ccgccaccat ggtgagtaag ggcgaggagg tcatcaaaga gttcatgcgc
     7321 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     7381 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     7441 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     7501 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     7561 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     7621 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     7681 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     7741 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     7801 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     7861 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7921 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc
     7981 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     8041 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     8101 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     8161 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     8221 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     8281 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     8341 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     8401 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     8461 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     8521 cgctacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     8581 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     8641 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     8701 tacaagagat ccttagagca gaaactcatt agcgaggagg acctgaacag cgaacagaaa
     8761 ctcatttccg aagaggatct caactccgag cagaagctga tcagcgagga ggacctgaga
     8821 tcctgagaat tcgacgatat ctctagagga tcataatcag ccataccaca tttgtagagg
     8881 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     8941 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9001 tcacaaattt cacaaataaa gcattttttt cactgcctcg aatcactagt ggggacagcc
     9061 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     9121 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     9181 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     9241 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgagtgcac
     9301 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     9361 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     9421 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     9481 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     9541 ctgaggcgcg ccggctagaa gatgggcggg agtcttctgg gcaggcttaa aggctaacct
     9601 ggtgtgtggg cgttgtcctg caggggaatt gaacaggtgt aaaattggag ggacaagact
     9661 tcccacagat tttcggtttt gtcgggaagt tttttaatag gggcaaatag gaaaatggag
     9721 gataggagtc atctggggtt tatgcagcaa aactacaggt atattgcttg tgatccgcct
     9781 cggagatttc catgaggaga taaagacatg tcacccgagt ttatactctc ctgcttagat
     9841 cctactacag tatgaaatac agtgtygcga ggtagactat gtaagcagat ttaatcattt
     9901 taaagagccc agtacttcat atccatttct cccgctcctt ctgcagcctt atcaaaaggt
     9961 atttagaaca ctcattttag ccccattttc atttattata ctggcttatc caacccctag
    10021 acagagcatt ggcattttcc ctttcctgat cttagaagtc tgatgactca tgaaaccaga
    10081 cagattagtt acatacacca caaatcgagg ctgtagctgg ggcctcaaca ctgcagttct
    10141 tttataactc cttagtacac tttttgttga tcctttgcct tgatccttaa ttttcagtgt
    10201 ctatcacctc tcccgtcagg tggtgttcca catttgggcc tattctcagt ccagggagtt
    10261 ttacaacaat agatgtattg agaatccaac ctaaagctta actttccact cccatgaatg
    10321 cctctctcct ttttctccat tataactgag ctatwaccat taatggtttc aggtggatgt
    10381 ctcctccccc aatatacctg atgtatctac atattgccag gctgatattt taagacatwa
    10441 aaggtatatt tcattattga gccacatggt attgattact gctactaaaa ttttgtcatt
    10501 gtacacatct gtaaaaggtg gttccttttg gaatgcaaag ttcaggtgtt tgttgtcttt
    10561 cctgacctaa ggtcttgtga gcttgtattt tttctattta agcagtgctt tctcttggac
    10621 tggcttgact catggcattc tacacgttat tgctggtcta aatgtgattt tgccaagctt
    10681 cttcaggacc tataattttg cttgacttgt agccaaacac aagtaaaatg attaagcaac
    10741 aaatgtattt gtgaagcttg gtttttaggt tgttgtgttg tgtgtgcttg tgctctataa
    10801 taatactatc caggggctgg agaggtggct cggagttcaa gagcacagac tgctcttcca
    10861 gaagtcctga gttcaattcc cagcaaccac atggtggctc acaaccatct gtaatgggat
    10921 ctgatgccct cttctggtgt gtctgaagac cacaagtgta ttcacattaa ataaataatc
    10981 ctccttcttc ttcttttttt ttttttaaag agaatwctgt ctccagtaga attactgaag
    11041 taatgaaata ctttgtgttt gttccaatat ggwagccaat aatcaaatac tcttwagcac
    11101 tggaaatgta ccaaggaact attttattta agtgwactgt ggacagagga gccataactg
    11161 cagacttgtg ggatacagaa gaccaatgca gacttaatgt cttttctctt acactaagca
    11221 ataaagaaat aaaaattgaa cttctagtat cctatttgtt aaactgctag ctttactaac
    11281 ttttgtgctt catctataca aagctgaaag ctaagtctgc agccattact aaacatgaaa
    11341 gcaagtaatg ataattttgg atttcaaaaa tgtagggcca gagtttagcc agccagtggt
    11401 ggtgcttgcc tttatgcctt aatcccagca ctctggaggc agagacaggc agatctctga
    11461 gtttgagccc agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca
    11521 gaaaccctgt tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct
    11581 aatgagccac tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat
    11641 tttttctgtc tctgctgttg gttgggtctt ttgacactgt gggctttctt aaagcctcct
    11701 tccctgccat gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt
    11761 ttgccttcta agggcagctg ctgagwtttg cagcctgatt tccagggtgg ggttgggaaa
    11821 tctttcaaac actaaaattg tcctttaatt tttttttaaa aaatgggtta tataataaac
    11881 ctcataaaat agttatgagg agtgaggtgg actaatatta atgagtccct cccctataaa
    11941 agagctatta aggctttttg tcttatacta actttttttt taaatgtggt atctttagaa
    12001 ccaagggtct tagagtttta gtatacagaa actgttgcat cgcttaatca gattttctag
    12061 tttcaaatcc agagaatcca aattcttcac agccaaagtc aaattaagaa tttctgactt
    12121 taatgttatt tgctactgtg aatataaaat gatagctttt cctgaggcag ggtctcacta
    12181 tgtatctctg cctgatctgc aacaagatat gtagactaaa gttctgcctg cttttgtctc
    12241 ctgaatacta aggttaaaat gtagtaatac ttttggaact tgcaggtcag attcttttat
    12301 aggggacaca ctaagggagc ttgggtgata gttggtaaat gtgtttaagt gatgaaaact
    12361 tgaattatta tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc
    12421 taagggatcc ctaggacttg ctgagcacac aagagtagta cttggcaggc tcctggtgag
    12481 agcatatttc aaaaaacaag gcagacaacc aagaaactac agtaaggtta cctgtcttta
    12541 accatctgca tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc
    12601 ccccatcaaa ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa
    12661 tgttggtttt gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag
    12721 aacaaagctg ctatttcgag gtctcttggt tatactcaga agcacttctt tgggtttccc
    12781 tgcactatcc tgatcatgtg ctaggcctwc cttaggctga ttgttgttca aataacttaa
    12841 gtttcctgtc aggtgatgtc atatgatttc atatatcaag gcaaaacatg ttatatatgt
    12901 taaacatttg kacttaatgt gaaagttagg tctttgtggg ttttgatttt aatttcaaaa
    12961 cctgagctaa ataagtcatt ttacatgtct tacatttggt gaattgtata ttgtggtttg
    13021 caggcaagac tctctgacct agtaaccctc ctatagagca ctttgctggg tcacaagtct
    13081 aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact agttatatgt
    13141 tggaggacat gtttatccag aagatattca ggactatttt tgactgggct aaggaattga
    13201 ttctgattag cactgttagt gagcattgag tggcctttag gcttgaattg gagtcacttg
    13261 tatatctcaa ataatgctgg ccttttttwa aaagcccttg ttctttatca ccctgttttc
    13321 tacataattt ttgttcaaag aaatacttgt ttggatctcc ttttgacaac aatagcatgt
    13381 tttcaagcca tatttttttt cctttttttt tttttttttg gtttttcgag acagggtttc
    13441 tctgtatagc cctggctgtc ctggaactca ctttgtagac caggctggcc tcgaactcag
    13501 aaatccgcct gcctctgcct cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg
    13561 ctaagttgga tattttgtat ataactataa ccaatactaa ctccactggg tggattttta
    13621 attcagtcag tagtcttaag tggtctttat tggcccttat taaaatctac tgttcactct
    13681 aacagaggct gttggactag tggsactaag caacttccta cggatatact agcagataag
    13741 ggtcagggat agaaactagt ctagcgtttt gtatacctac cagcttatac taccttgttc
    13801 tgatagaaat atttaggaca tctagcttat cgatccgtcg acggtatcga taagcttgat
    13861 atcgaattct accgggtagg ggaggcgctt ttccaaggca gtctgagcat gcgcttagca
    13921 gccccgctgg cacttggcgc tacacaagtg gcctytggcc tcgcacacat tccacatcca
    13981 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctwctcctc
    14041 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtsaggacgt gacaaatgga
    14101 agtagcacgt ctcactagtc tcgtcagatg gacagcaccg ctgagcaatg gaagcgggta
    14161 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
    14221 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
    14281 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
    14341 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
    14401 ttttgttatt cttctaatct tttgtatgga aaacttttct tcgtaccacg ggactaaacc
    14461 tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa
    14521 ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata
    14581 ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta
    14641 tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga
    14701 gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa
    14761 aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc
    14821 tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat
    14881 taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc
    14941 ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga
    15001 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    15061 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    15121 gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga tcagcctcga
    15181 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    15241 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    15301 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    15361 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    15421 gaaccagctg gggctcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
    15481 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    15541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    15601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    15661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    15721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    15781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    15841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    15901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    15961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    16021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    16081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    16141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    16201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    16261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    16321 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    16381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    16441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    16501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    16561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    16621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    16681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    16741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    16801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    16861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    16921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    16981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    17041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    17101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    17161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    17221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    17281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    17341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    17401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    17461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    17521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    17581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    17641 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
    17701 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
    17761 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    17821 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    17881 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    17941 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    18001 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
    18061 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
    18121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    18181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    18241 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
    18301 atagggcgaa ttggagctcc a
//
