LOCUS       sequence-461337-        5976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish FGFR1a receptor kinase domain fused to VfLOV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Iannucci LE, Thomas VSM, Murphy MR, Donahue CET, Anderson WK, Rogers
            CE, Saul AJ, Rogers KW
  TITLE     
  JOURNAL   bioRxiv [Preprint]. 2025 Apr 19:2025.04.17.649426. doi: 
            10.1101/2025.04.17.649426.
  PUBMED    40568079
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Apr 19:2025.04.17.649426. doi: 
            10.1101/2025.04.17.649426."
COMMENT     Sequence Label: pCS2-Opto-zFGFR1a
FEATURES             Location/Qualifiers
     source          1..5976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..147
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1969..1995
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2028..2162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2052..2071)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2106..2125
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2275..2295)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2275..2293)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2314..2330)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2330)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2327..2349)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2338..2354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2362..2368,2369..2386,2387..2392))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2407..2428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2545..2562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2716..3304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2796..2815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3475..4266,4267..4335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4098..4117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4336..4440)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4466..4921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4603..4624)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4815..4834
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4992..5976
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg ccaccatggg gagtagcaag
      121 agcaagccta aggaccccag ccagcgcggt ggaggaggtt ctggaggcgg tggaagtggt
      181 ggcggaggta gcctggccaa aatgcacagc tctgccaaga aaagcgactt caacagccag
      241 ctggccgtcc acaagctggc caagagcatc cccctgcgca gacaggtaac agtgtctgtg
      301 gactccagct catctatgca ttcgggtggg atgttggtcc gtccatcccg tctgtcctcc
      361 agtggctccc caatgctctc aggggtctcc gaatacgagc ttccccagga cccacgctgg
      421 gaggtgcaac gagacaggct ggttctcggg aaacctcttg gcgaaggctg ctttggacag
      481 gtgatgatgg ccgaggcgat ggggatggat aaagaaaaac ccaatagaat caccaaagtg
      541 gccgtcaaga tgctcaaatc ggatgccacg gagaaagacc tgtcagacct gatctctgag
      601 atggagatga tgaagattat tggcaaacac aagaacatca tcaacctgct gggagcctgc
      661 acacaagacg gtccgctgta cgtcatcgtg gagtttgctg ctaaagggaa cctgcgggag
      721 tatctgcgcg tacggcgtcc accagggatg gagtactgtt ataaccctga ccaggtgcca
      781 gtggagaaca tgtccattaa agacctggtg tcctgcgcat atcaggtggc ccgaggaatg
      841 gagtatctcg catccaagaa gtgtattcat cgagacctgg ctgctcggaa tgtgctggtg
      901 acggaagata acgttatgaa gatcgcagac tttggcctgg ccagagacat ccatcatatt
      961 gattactaca agaagaccac caatggtcgt ttgccggtga aatggatggc tcccgaagct
     1021 ctgtttgacc gcatatacac ccatcaaagt gacgtctggt cttttggggt gctgctgtgg
     1081 gagatcttca ctctgggggg ctctccgtac cccggcgtcc ccgtcgagga gctctttaag
     1141 ctgctgaagg aaggacaccg catggaccga ccctccacat gcacacatga gctgtatatg
     1201 atgatgaggg attgttggca cgccgtcccg tctcagagac ccacttttaa acagctggtg
     1261 gaggatctgg accgcaccct ctccatgacg tccaatcagg agtatctgga cctgtccgta
     1321 tctctggacc agttttctcc aaacttcccg gacactcgca gctccacctg ctcctcaggt
     1381 gaagactcag tgttttctca tgacgccgga gccgatgagc cctgtttgcc caaattccca
     1441 ccccatccca accgaggagt ggcctttaaa aagcgcggtg gaggaggttc tggaggcggt
     1501 ggaagtggtg gcggaggtag ccctgactac agtctcgtga aggctctgca aatggcacaa
     1561 cagaattttg tcattacaga cgcctccctc ccagacaacc ctatcgtcta cgccagtaga
     1621 gggtttctga cactgacagg ctattctctc gaccagatcc tgggcaggaa ctgcaggttt
     1681 ctgcaagggc cagaaacaga cccaagagct gtggataaga tcaggaatgc catcaccaaa
     1741 ggcgttgata ccagtgtctg tctgctgaat tatagacagg atggcacaac cttctggaat
     1801 ctcttcttcg tggctggact cagagattct aagggcaata ttgtcaacta cgtcggagtg
     1861 cagtcaaagg tgagcgaaga ttatgccaag ctgctggtca acgagcagaa cattgagtac
     1921 aaaggtgtgc gcaccagtaa catgctgcgc agaaagcccg ggtctagtta tccgtacgac
     1981 gtaccagact acgcataatc tagaactata gtgagtcgta ttacgtagat ccagacatga
     2041 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2101 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2161 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2221 tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta
     2281 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2341 ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg
     2401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4441 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     4501 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4561 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4621 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4681 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4741 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     4801 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4861 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4921 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4981 ctattacgcc agtcgaccgc caattcaata tggcgtatat ggactcatgc caattcaata
     5041 tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata
     5101 tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata
     5161 tggcggacct ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga
     5221 ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg
     5281 cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc
     5341 caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca
     5401 tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata
     5461 tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat
     5521 atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca
     5581 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc
     5641 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc
     5701 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta
     5761 acttggcaag tacattacta ttggaagtac gccagggtac attggcagta ctcccattga
     5821 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg
     5881 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa
     5941 tgggaggtct atataagcaa tgctcgttta gggaac
//
