LOCUS       sequence-461357-       13214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express METTL1 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:METTL1_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(255..271)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(255..271)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(326..344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(354..370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(363..385)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(511..966)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(648..669)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     860..879
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        993..1097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1098..1166,1167..1958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1316..1335)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2129..2717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2618..2637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2871..2888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3005..3026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3041..3046,3047..3064,3065..3071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3084..3106
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3103..3119
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3103..3119
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3138..3158
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3140..3158
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    10978..11112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11133..11267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(11569..12282,12283..12285,12286..12288))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(11572..12282,12283..12285,12286..12288))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11614..11635)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     11963..11982
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12222..12243
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
       61 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
      121 tgtgcattgt ggtaatagat gtgacatgat tgcattcatt ttatgtttca ggttcagggg
      181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      241 gatcctctag atcagatcca ctagttctag agcggccatt accctgttat ccctaggccg
      301 ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
      361 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      421 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      481 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
      541 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
      601 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
      661 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
      721 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
      781 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
      841 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
      901 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
      961 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1021 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1081 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1141 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1201 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1261 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1321 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1381 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1441 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1501 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1561 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1621 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1681 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1741 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1801 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1861 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1921 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1981 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2041 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2101 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2161 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2221 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2281 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2341 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2401 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2461 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2521 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2581 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2641 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2701 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2761 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2821 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2881 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2941 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3001 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3061 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     3121 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg ggtaccgggc
     3181 cccccctcga attaccctgt tatccctatc gagcaaaatc aatcctaatg taaaatagta
     3241 tacgttcatc attataatgc aaacactgta gaactgttgt gttgtgcctt gcatttcatt
     3301 ctcagtttct tatcgtctgt ctctttttac gacagagcta gaaaaaacta gccttcattt
     3361 gccatcgccg tatgcaaatc cactccagaa ctcaacaggt ttgttctgcg gcgggtgtaa
     3421 gtgtgaacac cggcatgtaa gtgcgcttga gcaggagaat aaagcgctca gagacaccgg
     3481 aggaatcctt tattttgcgt tgcgcctcag gggattcgct gagccttctc ttcatggccc
     3541 tgctcttcag acacaggtgc tccgctgttt gtgtttttca ctgttcttca ggcactcgtg
     3601 gttacgggtt tttgttttgc actcttgcac acacatctct cggcctccaa aacaaacaca
     3661 caactgcacg agggctcgaa gcccccaccg aaaaacaaaa acattatgct aatgaggaag
     3721 gcacagctgt tcccttcttc actctctctc tcgttctttc aacaacagtc gcctaatttc
     3781 tctcgttcct ttaaagagcc gccaagcaga aacagttaat gcaaatataa gcctgcggga
     3841 aataaggaga atttttacaa aatccgagct tcccctcctg ccaaaatgtg atgtgtgact
     3901 cacgcattcg atatttggtg taatgagagt gaatcattct gctccacacc gaacccgatg
     3961 ccacagacca ctcagatgca caccgaggag actattttaa cagcacatta catccagact
     4021 gtcacagatg cattaccaag agtgtgattt cagtgtcgaa tgcaggtctt aaaggaaaat
     4081 ctgttgcgat tgcatgtgcg ctaactagac acgaccaacg aatgggaaca atctgtgaaa
     4141 tattatacaa tcacaggcaa ttaaaagatg tttaaagttc aatttagttt taattaatat
     4201 gttatataaa actaatctgt tttaaatgta tatttatgat tttagcttta gctagcctct
     4261 tgaccagcag ctgaagtgtc cggcaagtac ctaaaaggga gaccacatgg gaaagctagg
     4321 ttgctcccgg aagaggtgtt agtaaaccag caggtctacc tgtggtctgt gtgggtccta
     4381 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     4441 taaaccagca ggtctacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
     4501 gtcaacctgt agtctgtgtg ggtcctaatg ccccagtata aaccagcagg tctacctgtg
     4561 gtctgtgtgg gtcctaatgc cccagtataa accaacaggt caacctgtga tctgtgtggg
     4621 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
     4681 cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc
     4741 agcaggtcaa cgtgtggtct gtgtgggtcc taatgccctg gtataaacca gcaggtcaac
     4801 ttgtggtctg tgtgggtcct aatgccccgg tataaaccag catgtcaacc tgtggtctgt
     4861 gtgggtccta atgccccggt ataaaccagc agatcaacct gtggtctgtg tgggtcctaa
     4921 tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccggtac
     4981 aaaccagcat gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg
     5041 tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt ctacctgtgg
     5101 tctgtgtggg tcctaatgcc ccggtacaaa ccagcatgtc aacctgtggt ctgtgtgggt
     5161 cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc
     5221 agtataaacc agcaggtcta cctgtggtct gtgtgggtcc taatgcccca gtataaacca
     5281 gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtctacc
     5341 tgtggtctgt gtgggtccta atgcaccagt ataaaccagc aggtcaacct gtggtctgtg
     5401 tgggttctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtccaaat
     5461 gccccagtac aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
     5521 aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccggtacaa accagcatgt
     5581 caacctgtgg tctgtgtggg tcctaatgcc ccggtataaa ccagcagatc aacctgtggt
     5641 ctgtgtgggt cctaatgccc cagtataaac cagcagatca acctgtggtc tgtgtgggtc
     5701 ctaaagtccc aggataaacc agcatgtcaa cctgtggtct gtgtgggtcc taatgccccg
     5761 gtataaacca gcagatcaac ctgtggtctg tgtgggtcct aatgccccag gataaaccag
     5821 caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtcaacct
     5881 gtggtctgtg tgggtcctaa tgccccagta tattgatgga gactctatac tgctcggtga
     5941 gctccatctt tcggataaga tgttaaaaca aggtcccgac tctctgtggt ggtaaaaaat
     6001 tcccaggatg tccttcctac cctggcatcc tggacaaatt tgctcactgt cctctgtcca
     6061 tcatggcctc ctaaccatcc ccatatcata attggcttta tcactctagc tggggccgaa
     6121 atcgcatact tccattttat gaggtacgct aaaatcagta tgcgagccaa gtaatatgtc
     6181 cgaatacttc cggcgagatt ctagagtgtg cattcgatgc acgctacgct attccatgat
     6241 gccccgtgag agaattcttg aatcggagag aagcaacgca actcatgcgg gtaggttacg
     6301 tgacgatgac atattggcgg atgtagtacg tccaagttcc attcatactg ctcacattca
     6361 tattttatag aatgttcttt tcccacagcc aagtagtaag tttaaattca aatacagtac
     6421 ctactaagta gtagtagacg gtttcggacg cagcccctgt ctcctctcca ccaatatact
     6481 ggtgtgtagt gtgcggtctg gtgcaatatg gctgccatcg cgtcatccag gtggatgctg
     6541 cacactggtg gttgatgagg agattttgcc caaaaaagtg ttttgagttt ccagaaaagc
     6601 gcgatataaa tgtaaggaat tatcattagc ttattataat ggaactttct ggataacttg
     6661 tgccagagct aggggccatt aaaactttgc cctggggtac ctcagggagc tgcaaggaag
     6721 tgctataggg ctaatgaaat gtttggggga aaaaaaaaaa ttttataagt aaaaataaaa
     6781 ctaaaataat taataatttc attgttgaaa attatagtaa aattgataaa caagataagc
     6841 agcattagta taaaaaacta aaaacaaacg taatttaaaa taattaaatt gttttaaata
     6901 aattatacaa aataatgttt acaacgacac ggtggtgcag taggtagtgc tgtcgccagc
     6961 aaagcaagaa ggtcgctggt tcgagcctca gctggatcac ttgatgtttc tgtgtggagc
     7021 attcgcgtgc gtttaacccc agattaataa agagactaag ccgaaaagaa aataaatgta
     7081 tgaaaatgtt tacgtaaaat gtaaataaat gcatgattaa taaaatatat gaattttaaa
     7141 gattgaggta gaattgtttt attattcaca catttggtta tttttaatca gtgacaactt
     7201 gtgatgctct ttatattgtt tattacgcta ctttgaatat tcagtctcaa attgcatgta
     7261 aatacgactt caaagcagaa ttagtttaat ttaattgcct gtaaacagtg ttgtactttg
     7321 atggtgattg tctgataata atatgatttc actatttaaa atttgaaaaa aaaaaatctg
     7381 aaaaatactc accggccact ttattaggta cacctgtcta actgcttgtt aacgtaaatt
     7441 tctaatcagc caatcaccag gcagcaactc agtgcattta ggcatgtaga catggtcaag
     7501 acgatctgct gcagttcaaa gcgagcatca gaatggagaa gcaaggtgat ttaagtgact
     7561 ttaaacgtga cgtgtttgtt ggtgccagac gggctggtct gagtatttca gaaactgctg
     7621 atttactggg gttttcacac acaaacatct ctggggttta cagagaatgg tccaaaaaaa
     7681 gataatatcc agtgagcggc agttctgtgg gggcaaatgt cttgttgatg ccagaggtca
     7741 gaggagaatg gcctgactgg ttccagctga tagaaaggca acagtaattc aaataaccac
     7801 tcgttagaac tgaggcatgc agaagagcac ctttgaacac aacatgtcga accttgaggc
     7861 gggtgggcta cagcagctca agaccacatc gggtgccact cctgtcagct aagaacagga
     7921 aactgaggct acaattcgca caggctcaac ataagtagac aatagaagat tgaacattat
     7981 gttgcctggt cagatggata cattctacct tgtatcaaca gttcaggctg gtgatggtgg
     8041 tgtaatggtg tgggggatat attcttggca cactttgggc ccattagtac caattaacca
     8101 tcgtgtcaac gccacagcct aactgagtat tgttgctgac catgttcatt tctttttgac
     8161 cacagtgtac ccatcttctg atggctactt cctgcaggat aatgcaccat gtcataaagc
     8221 gtgaatgatc tcagactggt ttcttgaaca tgacaatgag ttcactgtgc tcgaatgacc
     8281 tccacagtca ccagatctca atccaataga gcgcctttgg ggtgtggtag aatggaagat
     8341 tcgcatcctg gatgtgcagc cgacaaatct gcatgatgct agcatgtcaa tatggaccaa
     8401 aatctctgag gaattttcca gtacctggtt gaaatgcaaa aggaagggaa tctatgctaa
     8461 ggcaaaaaga ggtccaaccc agtactagtg gggtgtacat aataaagtgg ccggtgagtg
     8521 tatattgtta aggatatcta gataatccaa ataatttaca gcactattta aaaaaaaaac
     8581 tttatgtcac caagtctgca tttatttgat cacaattaca gtcgaaaaag caatatcggg
     8641 atacattgtt tccatttaaa ataactatat tatattttaa atatttttga caatgttatt
     8701 tctttaatgc caaagctaaa ttgtattcaa tctgaatttt agtgtcacat gatccttcag
     8761 aaaatattaa cacattgcta aaaatgtata ttattatcaa tttggatttt tttttttttt
     8821 ttgtatatta acctatgcta ttggttacta tacaaaagta ttactgtgaa tatatgcata
     8881 tacagaaccg tatcaagcgc ggggggtggt tgggttatgt ccccatttgg aaagacctgg
     8941 aaacattcat aatgactaaa attgcacgga aaaatataat atctatgagt attgctgatc
     9001 agcagcacaa aaccagaact ccatattaat aaacagccaa tgatgcagtg agtttgtgca
     9061 tgttttcatt caaactcgtg tgattgtgct caaacacctg ttgtgagcag cagttgcaaa
     9121 cagtaaacct caagaggagc cagtcaacct tcacaaacac tcaacctctg gctcactcac
     9181 taaacaattc gcgttaatat ctaaaataaa aatttccagc accaaacagc tctcagattt
     9241 cggagtacac tgaccaagag gtaagaagta acgttacatt actaggacga ctattgtaag
     9301 actagatatg taatatttgg aagcgtacgt gttttaagtg acatatagag tagaggaact
     9361 gatggaaata cgatttgcat aatgtaaaat gatatatatt aaaatatagg ctacagtagg
     9421 taacgtggtt tacatataac gttactgtgt taatgtagtg ctcagttact cgtgttgaca
     9481 gttattatta gtgcttgcta atttgtttgt taatgcttct cttgcgtatt tgattacctt
     9541 caggcgaatc taaaacatat cagatgtaga aacggcagaa aaatgttgaa gaaaatacga
     9601 gaatagtagc ttttggagca catagaaaag tacatcagat aaagcctacc gtttaaacgc
     9661 agacgccgct ctcgcactga atgctgggta ctgtagtcct tatattgtat gcttaatgtg
     9721 cgtctcaacg ggaaactaga gttaggaaaa ctacaatttc ctgtggaaaa tgtttctgtt
     9781 ttcggggaaa cagcgaaaga aagatatggt tccgcgtttt tttcttactt tgaataataa
     9841 aataagacct aactacacac ttcgcttact cttatgctaa tttgaagcac tagcattctt
     9901 tgatacaatg ataatcattt aaacatcaca gttatactac aaatactatg gttaaactat
     9961 gtctagtgta gtgtaacact atggttgcca ttgttatctt taagtttaag tattatctgt
    10021 ggtaaagcca tggttggttt ttataagagg taataaaatt aacattgtac aattaaatga
    10081 ctataaatta atgttttttt ttattttttt tacacagcta ccttcaaaaa gggatccatg
    10141 gcagccgaga ctcggaacgt ggccggagca gaggccccac cgccccagaa gcgctactac
    10201 cggcaacgtg ctcactccaa ccccatggcg gaccacacgc tgcgctaccc tgtgaagcca
    10261 gaggagatgg actggtctga gctataccca gagttcttcg ctccactcac tcaaaatcag
    10321 agccacgatg acccaaagga taagaaagaa aagagagctc aggcccaagt ggagtttgca
    10381 gacataggct gtggctatgg tggcctgtta gtggaactgt caccgctgtt cccagacaca
    10441 cttattctgg gtctggagat ccgggtgaag gtctcagact atgtacaaga ccggattcgg
    10501 gccctacgcg cagctcctgc aggtggcttc cagaacatcg cctgtctccg tagcaatgcc
    10561 atgaagcacc ttcctaactt cttctacaag ggccagctga caaagatgtt cttcctcttc
    10621 cccgacccac atttcaagcg gacaaagcac aagtggcgaa tcatcagtcc caccctgcta
    10681 gcagaatatg cctacgtgct aagagttggg gggctggtgt ataccataac cgatgtgctg
    10741 gagctacacg actggatgtg cactcatttc gaagagcacc cactgtttga gcgtgtgcct
    10801 ctggaggacc tgagtgaaga ccccgttgtg ggacatctag gcacctcaac tgaggagggg
    10861 aagaaagttc tacgtaatgg agggaagaat ttcccagcca tcttccgaag aatacaagat
    10921 cccgtcctcc aggcagtgac ctcccaaacc agcctgcctg gtcactgaat cgatgatgat
    10981 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
    11041 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
    11101 aataaacaag ttaacaacaa caattcgatg atgatccaga catgataaga tacattgatg
    11161 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
    11221 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
    11281 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
    11341 acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctct tgtttattgc
    11401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11521 ctacgtaata cgactcacta tagttctaga ggctcgagag gggccgcttt acttgtacag
    11581 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
    11641 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
    11701 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
    11761 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
    11821 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
    11881 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
    11941 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
    12001 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
    12061 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
    12121 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
    12181 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
    12241 caggatgggc accaccccgg tgaacagctc ttcgcccttg ctcaccatgg tcactgtctg
    12301 ctttgctgtt ggtctgggct cctgggtcac tggcttacta atggagtctt tatgtatgag
    12361 gactcttatc aattgttctt ctataaaggt ctgcagtgtt tctgttcgtc ccctacatgg
    12421 acacccagag cctcctaaat acaggagccc tgataactgc acaagtgctc agattccagc
    12481 agggtggaaa tgagataaag tgtgcagatg gggaggggga cgtgaatgag agatttgagg
    12541 gatgaaaagg atggatgaac gcattgaaaa tagccccttt cacaaaataa taccagtaaa
    12601 ttgtcatata attaatgacc agacctttac ctgtaaatga tgtgatgttc atatacagaa
    12661 gaatgccaac aatttacaga tgattttaca aattcttaat tttccttttc taaatggatt
    12721 tgttcacaca ttatctcttc aaagcaattg aattttctgg aaaagattgg ctgtgtgtgt
    12781 aaaaggtgct actgaggaat ttaatgtgac atggaatgaa gcaaacagca gcctaagaca
    12841 ggatggcagg aaaaatgtca catgtttaaa ataagagtga aaccaacgac ctggacttat
    12901 agagactgtg tgtccttagg caaagctctt gtacaaacag tgcttgtgac tgtgaaattc
    12961 ggggtttgcc tggattgtgt taaatgtgtg cttggatgta tcataaaaaa gaatcggttc
    13021 tctgtatttg acattttctg taatttatct aaagttacaa ctggctatgc ctgattttta
    13081 tcactattga atcaatcatt ttaaagaatg ttaagtctgc tgatttggag agctcattta
    13141 catttatttt gaatgtctct tatttaacac aactgattta agctttagat ctgcgaagat
    13201 acggccacgg gtgc
//
