LOCUS       sequence-461361-        9352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral helper virus for G-deleted rabies tracing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Shin D, Urbanek ME, Larson HH, Moussa AJ, Lee KY, Baker DL, 
            Standen-Bloom E, Ramachandran S, Bogdanoff D, Cadwell CR, Nowakowski
            TJ
  TITLE     High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping 
            Using Single-Cell and Single-Nucleus Sequencing.
  JOURNAL   bioRxiv [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167.
  PUBMED    39713304
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 
            10.1101/2024.10.01.616167."
COMMENT     Sequence Label: pSico-syn-GFP-TVA-oG-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..9352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             337..381
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    679..796
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    854..1078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1193..1781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1299..1310
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1708..1728
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3407..3466)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(3953..4006)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(4016..4726,4727..4729,4730..4732))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4016..4726,4727..4729,4730..4732))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4058..4079)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4407..4426
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4666..4687
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4729..4738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4753..5200)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     LTR             5593..5773
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5835..6423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5915..5934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6594..7385,7386..7454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7217..7236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7455..7559)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7634..7653)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7825..8204
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8206..8404
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8359..8379
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8422..8602
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8649..8774
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(9271..9352,1..152)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
       61 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
      121 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
      181 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
      241 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
      301 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
      361 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
      421 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
      481 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
      541 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      601 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
      661 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      721 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      781 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
      841 gggcccgctc tagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
      901 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
      961 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     1021 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagct
     1081 cgagtagaca tgtcgagcta gccaatgatc atacggcgcg ccgaaggtac ccttggatcc
     1141 ctacctaggt gggaattcgc ttgtgcccca gtttgctagg gaggtccagg acgcggggag
     1201 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     1261 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     1321 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca aggaaaggac
     1381 gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     1441 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     1501 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     1561 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     1621 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     1681 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     1741 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tggatcgtac gtcggcataa
     1801 gctttgtagc ggccgctaca aattacagtc gggtctcacc accagactta tggctctccc
     1861 aggaagatat gattttcccg gattgtggag ttacagacac ttcccgacct gtccctcgca
     1921 ggttatgttg ggttggttcg gatctgttca ctctccgcca gcacgtcatg aggaatatta
     1981 tcagcatgag ggcggtcaac gctccggcac tgagaagaac gtacttgccc cagtttggaa
     2041 gcccgaggtc taccccactg attcgttcat gtacatctgg caagtggacc tctacaaagt
     2101 cttcggcctc atcaccattt ttgaataccg tactggggtc tgcgagagga tgcataagtg
     2161 gtatgacgga agaaacgagc aactccatgt gttgttgcaa aagggagctt tgcatctctg
     2221 gaatcagcac gttgccgtcg gggccaagga taataccatt gaagaatacg ccgtttacgt
     2281 gggggtggca tctaccacca acccgcaagc accccttgga tggtatgatc tcgttccaag
     2341 tccgaacaga cttgtaatgc gcgtcagcct ccatgagagt tttgttgaaa attgtatatg
     2401 ctttcccaaa gcctggtaca agtttcctaa gatgggacaa ccggcggaaa gaaacagatt
     2461 tagtagtcat aatagattcg agagcgtcca ggcactcttc tctcttcttc acaagttctt
     2521 caacgaccaa atgttctatt tcatcactcc ggaagtcgtg gagattcacc agctggcctg
     2581 gtggacacca ctttgtctcg ttggaagttt gcatcgccac ccaggttcca tccatcaacc
     2641 gaagaccaag gacgccgcag agtttgagtt tgcaggcacc tttcaatgat ttgtaaaggc
     2701 ccctttcatc tacaaatcca cacgtctcac tacccttgga cgcccgcttg ccgcgagagt
     2761 tagtgaatat gtcacaactc atacccaacc tgggattctc cggcatccag atcgtgtaat
     2821 catggttggt gctgcaatag gtggaactca ccgctacgcc gctacaattg ccgcccggga
     2881 acacaggact gtgcaggctc cggtcgtaag ggtccagatc tgcgactgag ggactaatga
     2941 ttacgaggga ttcttttgtc gttttcacag ttcgcaacca atggtaatct gggtagggat
     3001 tatgcagtga ctcttcatac ctcggatcgc cagccatttt ccaattgtaa gctgctcggc
     3061 acgcgtcagg cgttggtcta aaatgttttc ttttaaacgt ggtggttaca tacccgacaa
     3121 agttagtgta tgtctcagct tcagttacaa cacctgtaca cgtaaaacca ttcattttga
     3181 tggctgaaat ataccctacc ttgagctcca tataggagaa cccactcaag ttggtgcatc
     3241 cctcatcttc tactaccagg ttgttagggc aagacaaatg gtggatgtcg ataggagacc
     3301 acggaccgag cttatccggt atggtgtaaa tagggaattt accaaagcac agtgggaaca
     3361 ccaacagagg aacgaacaac agcgcttggg gcaccatggt gcctgcagga cctgggtttg
     3421 actcgacgtc tccggccaat ttgagcaagg cataattcgt gcactgtcct gaaccactga
     3481 acaggtccgc ctgactctta tccgggacga gaagttcctt agaagctgac tccaatgaaa
     3541 aaatatcaga gcggctcttt gctttggact tgccccatgc ggctatcccc ccgacggcta
     3601 cgagacaaca gagcaaaacc gctgttatca gcatccacat ccgtccgtga ttccgtgcgg
     3661 gaagcgcccg acctggagct ggagccgtgg gtgcctcagt tccgttatcc gtaggcaccg
     3721 caggaattgc acttgtacca cagccccatt catctcttcc atcgtcgcag tctggatgcc
     3781 cgtcgcaaag ccaatcctgt gggtaacatt ccccatgtgc gcccgggggc tcactacacc
     3841 tgaattgacc tggtggacac cgggacaatg atccattacc tgagccgttc cccgttacat
     3901 tgcctggcag caggagcaga agaagggctg gaagaagcct tgccatcgtg cgaggcccag
     3961 gattctcctc cacgtcacca cacgtaagca aggaaccgcg cccttccccg gaccccttgt
     4021 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     4081 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     4141 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     4201 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     4261 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     4321 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     4381 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     4441 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     4501 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     4561 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     4621 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     4681 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga
     4741 ccggtttctc gactgcgctc tcaggcacga cacgactcct ccgctgccca ccgcagactg
     4801 aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc
     4861 gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac caaggtggcc
     4921 gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg
     4981 gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg aagctggcag
     5041 tgcgcacgcg cctcgccgca tcctgtttcc cctccccctc tctgataggg gatgcgcaat
     5101 ttggggaatg ggggttgggt gcttgtccag tgggtcgggg tcggtcgtca ggtaggcacc
     5161 cccaccccgc ctcatcctgg tcctaaaacc cacttgcact ggtgaccttt aagaccaatg
     5221 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5281 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5341 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     5401 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     5461 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5521 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     5581 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5761 aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6241 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7561 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     7621 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7681 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7741 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7801 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     7861 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7921 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7981 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8041 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     8101 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     8161 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     8221 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     8281 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8341 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     8401 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8461 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8521 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8581 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8641 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8701 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8761 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8821 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8881 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     8941 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9001 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9061 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     9121 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     9181 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9241 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9301 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca ga
//
