LOCUS       sequence-461363-       11103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentivirus-mediated co-expression of GFP and DGCR8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Shin D, Kim CN, Ross J, Hennick KM, Wu SR, Paranjape N, Leonard R, 
            Wang JC, Keefe MG, Pavlovic BJ, Donohue KC, Moreau C, Wigdor EM, 
            Larson HH, Allen DE, Cadwell CR, Bhaduri A, Popova G, Bearden CE, 
            Pollen AA, Jacquemont S, Sanders SJ, Haussler D, Wiita AP, Frost NA,
            Sohal VS, Nowakowski TJ
  TITLE     Thalamocortical organoids enable in vitro modeling of 22q11.2 
            microdeletion associated with neuropsychiatric disorders.
  JOURNAL   Cell Stem Cell. 2024 Mar 7;31(3):421-432.e8. doi: 
            10.1016/j.stem.2024.01.010. Epub 2024 Feb 20.
  PUBMED    38382530
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2024.01.010"; journalName: "Cell Stem Cell"; date: 
            "2024-03-7- 7"; volume: "31"; issue: "3"; pages: "421-432.e8"
COMMENT     Sequence Label: pSicoFlipped-CAG-GFP-2A-DGCR8-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..11103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        693..1072
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             1481..1661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(1723..2311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1803..1822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2482..3273,3274..3342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3105..3124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3343..3447)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3522..3541)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3713..4092
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4094..4292
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4247..4267
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4310..4490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4537..4662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5159..5392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5577..5621
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5919..6036
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    6094..6318
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6420..7008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6526..6537
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6935..6955
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9370..9423)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(9433..10143,10144..10146,10147..10149))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9433..10143,10144..10146,10147..10149))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9475..9496)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9824..9843
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10083..10104
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      10146..10155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10241..10263)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cgacggcaca ggaccgaggg gcacccacga agcggaggag ggctgggacg cggccagaga
       61 aaagtcacac gccatcttta aaagaagggc ggaggacagg cggggggggg agggcggtgc
      121 cgcacgctcg cgtgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgagggg
      181 gsggcggagc ggggcgcggg gcgaaggcag cgagcgggga ctgccgcccg gggcgggcgg
      241 cggtttttat agggccgccg ccgcccccgc cccgccataa aacgaaacct tcggagcggc
      301 ccgctctggg tggccgccgc cgcccctcac cgcccccgcc cccccccccc cccccccccc
      361 cccccccccc ccccgcgccc ccggagcgag scgcccnccc ccacgcgccc gctbttyytt
      421 tttgagggsg cccccccccc cccccccccm cccaaaaaag aaaaaccyty csggggcgcg
      481 ccgccccctg tcgtntgygs cccccccccc cccccccccc cccccccccc cccccccccc
      541 cccccccccc ccccccccct ggngcgcgcc cccccccccc cccccgcccc catcgctgca
      601 caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg
      661 agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag
      721 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
      781 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
      841 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
      901 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
      961 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     1021 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgagctgat
     1081 ctacgatgta gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     1141 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     1201 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     1261 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     1321 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     1381 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     1441 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     1501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1621 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca
     1681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     3481 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     3541 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     3601 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     3661 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     3721 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3781 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3841 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3901 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3961 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4021 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4081 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4141 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4201 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4261 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     4321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     4501 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     4561 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     4621 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     4681 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     4741 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     4801 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     4861 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     4921 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     4981 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     5041 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     5101 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     5161 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     5221 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     5281 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     5341 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     5401 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     5461 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     5521 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     5581 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     5641 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     5701 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     5761 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     5821 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     5881 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5941 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     6001 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     6061 gatccagttt ggttagtacc gggcccgctc tagccataga gcccaccgca tccccagcat
     6121 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     6181 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     6241 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     6301 ggcaactaga aggcacagct cgagtgcgtg cggattagtc tagcggtgaa gtggctgaag
     6361 atcctcccgc gcagtaagcg aattcgcttg tgccccagtt tgctagggag gtccaggacg
     6421 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     6481 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     6541 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     6601 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     6661 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     6721 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     6781 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     6841 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     6901 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     6961 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgg atcgtacgtc
     7021 ggcataagct ttgtagcggc ctcacacgtc cacggtgcac aggggctcac cgccaggctg
     7081 ggcggacgcc acaatggaca tcttgggctt ctttcgagtc tcctcccttt cctcagctag
     7141 cctcttcatc tcctcttgga gcttgctgag gatgtgcagg ttgggcttgt tcttcttggc
     7201 atactgctgc agctcaatca cactcttgtc cgatgtctcc tgcttgacca tcttgctgct
     7261 ctcacggcca tacatgcgca gtaaagaccc ccagttcttg acatgtgggt gcagcagctg
     7321 aaggatcttc tgtgaggcta actgctttcc aactctcttg ttcttacacc acccgcgcac
     7381 tgtgtgcttg ccacacgcca tgacgtattc actcttctgg tttttcccag gaaccacttc
     7441 aaacttgata gacgtgtcac ccatcccatg gtttctttta aggcactcgt ggaggatctg
     7501 atatggagac aacagcccag ccttgctggt cagctcgtag acccgcgagt cctcgatgct
     7561 gatgtggtta aaatactcga gttcttcact gtctttgggc ttctcttcag aggtctgttt
     7621 aacaaagtca gggatgagga tttccagtgt agctcgggca gctttattct tcgcaagttt
     7681 tttgctgctt gcagttccag atccgtaagt cacaccatca atggtcaccg aggcaccaaa
     7741 aggctcactt gggttctcac attcaaagaa attatagaca gggcggacct tgaggacacg
     7801 ctgcatgtac tcgtgcagga tgcagacctc ggatttcccg ttggggttaa taacaaactc
     7861 tttctttgtg ggtgcatctt gcactgataa agtaatgagc ttctgattgg ctggcaagat
     7921 gggcctctcg gactccgcct gcttccgctt catttcccga ttgaattgcc gccgctcagc
     7981 ccaagtcctg aattttttca cagtaacttg ctcaaagtca aaacgcttct ccaggtagct
     8041 tcgaaattcc tcgagatcaa cggattcatc tttgcacacc tcgactttgg ccttcacctg
     8101 ccccagggcc ccaggggctg cctcagcccc tagtgggtct ttctcgtccg ggggccccgg
     8161 gtcagcaccc atagagtcag gctcatccag gggaaactcc agctctgcag atcggctcag
     8221 gggcttgacg ggggacacat ccccactagg ggtgaggtca ctgctttgct cccgttcctc
     8281 gttgtccttc attttcttat aatgcagaca agggatgcta ctcagaggag ggtcgtgttt
     8341 ccgtatgctt cccgttccca agaagtatgg cctggaccag gtgaccaccc gagactctct
     8401 gtgtaggtac accgggactc cagagttatg gaatgtcatg atccacccgt cgggcagcgg
     8461 ctctgtaggt gggcggccac gacttttgag cactgtttta atcttggtca tcatcggctg
     8521 cacacttgtc tctccatcgg acggatggtc gctgtctccg ccatattttt cctctgttcg
     8581 cctctttttg ggggcacaaa ggccttcttc cagcagagca tccacatcgt tgtcaaagtc
     8641 atcctcgtag gggaaattca aggcctcttc atccactctg tctctctgaa cgattgcttt
     8701 agccgtgaac ccgcctgctc cttcttcatc tagctccaaa ttgtcagtaa aatcttctaa
     8761 ctcatcgagc actgcatact ccactctctt ttcctgatcc agctcattct cctcatcctt
     8821 cttatcagca ctctcacccc ctatgcctac cccgtcacca acactcccgc caaagggaca
     8881 agcatgcacg tccccactga cagggctaag gagcagaccg cactccgccc gcacgtcgcg
     8941 ctctgctcct gtgtacagca ccttcctgtc cttactcctg cagctctcgg taaagctcac
     9001 gctaatcttt acatccttaa gcaacttaag gtcaggggag aacttccgga ccgcaggtgc
     9061 gtgccgggcg gtgcgcgggc tgtggccact acagttcggg tctatgagga ggcggccctt
     9121 agagaaggat cctttggaga gaagagaagc tccgtagaag ttgaaggggt cctcagcagg
     9181 gagttcggac tgtccatcac caccagagcc aacgtccatt acctctgcac cactggacgt
     9241 ttgcagggga ggtggtggag actgctcacg gggcagcgct tggaaggggc gagctcggct
     9301 ctccatcacc gcttctcctg cgggcccaca cgggagcgga gaggggctct catctgtctc
     9361 catgtcgaca ggcccaggat tctcctccac gtcaccacac gtaagcaagg aaccgcgccc
     9421 ttccccggac cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     9481 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     9541 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     9601 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     9661 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     9721 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     9781 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     9841 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     9901 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     9961 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
    10021 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
    10081 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
    10141 gctcaccatg gtggcgaccg gtagcgctag ttacgactag caggagctgt aggaaaaaga
    10201 agaaggcatg aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga
    10261 accccgccct gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgaggct
    10321 ggagagggag aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct
    10381 tcctgccggc gccgcaccgc ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc
    10441 cagatttcgg ctccgcacag atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt
    10501 accataaaag gcaatggctg cggctcgccg cgcctcgaca gccgccggcg ctccgggggc
    10561 cgccgcgccc ctcccccgag ccctcccccc gcccgcacgg cggccccccc ccccccgccc
    10621 cccccccccc cccccccccc cccccccccc cccccgcccc cccccccccc cccccccccg
    10681 cgcgcccccc ccccccaccc cgccgcccgc ccccccccnc cccgcgcgcg nngggggggc
    10741 gcgggggggg gssgccgcct cgcccccccc cccccccccc cccccccccc cccccccccc
    10801 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccgcgctgg
    10861 cgcgggggcg ctgcggcggc ggcgaggggg ccgggccgcg ggcgggtggc gcagcgcacg
    10921 gacggtgcga ggcgccgccc ggcgcgccca ccggggacac aggggccgcg aggcggccct
    10981 caggggtgtg ccgcggccgc agagcagaaa ctagccgtca gtagcccacg cgcgagtgga
    11041 ggcggggggg agaagggggg tcccgcgggc tgagcggggg gaggagcggg gtcggtcagg
    11101 ggg
//
