LOCUS       sequence-461386-        9408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward 
            DI, Bustos F
  TITLE     RLIM-specific activity reporters define variant pathogenicity in 
            Tonne-Kalscheuer syndrome.
  JOURNAL   HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378.
  PUBMED    39482882
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "HGG Adv."; 
            date: "2024-10-31"; volume: "6(1):100378. doi"; pages: " 
            10.1016/j.xhgg.2024.100378"
COMMENT     Sequence Label: pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     979..998
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        979..997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1007..1386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1388..1663
                     /label=chicken beta-actin promoter
     intron          1664..2680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2604..2626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2688..2707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2743..3459
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    4352..4923
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4518..4535)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4745..4764
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4932..5642
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5077..5095)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    6003..6137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6040..6059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6094..6113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6142..6641
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6177..6200)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6574..6593
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6657..7256
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6657..6676)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7153..7173
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7592..7609)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7592..7608)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7601..7623)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7749..8204)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7886..7907)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8098..8117
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8231..8335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8336..8404,8405..9196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8554..8573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9367..9408,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      961 tttggccaag tcggcctcta atacgactca ctatagggag ctcgtcgaca ttgattattg
     1021 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1201 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1261 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1321 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1381 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1441 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1501 gggggggsgc gcgccaggsg gggggggggg gggsgagggg gggggggggg sgaggsggag
     1561 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1621 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1681 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1741 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1801 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1861 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1921 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1981 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     2041 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     2161 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     2221 gccgggcggg gggtggcggc aggkgggggt gccgggcggg gcggggccgc ckcgggccgg
     2281 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2461 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2521 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2581 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2641 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     2701 tattgtgctg tctcatcatt ttggcaaaga attcaactcg agatggtgag caagggcgcc
     2761 gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac
     2821 aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2881 ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac caccctgagc
     2941 tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag
     3001 agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac
     3061 tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg catcgagctg
     3121 accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac
     3181 aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa ggtgaacttc
     3241 aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta ccagcagaat
     3301 acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc
     3361 gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg cttcgtgacc
     3421 gccgccgcca tcacccacgg catggatgag ctgtacaagg gaggcggagg cagcatgaat
     3481 gaacaaaaaa tgaatgaaca aatgaagaaa acggcaaaga caagtggcca gaaagggccg
     3541 ggcggaagag ccctcgacag actgacccta aagcaagacg aggcaaggcc agtccagaat
     3601 accagagtgg aagctccccg tgtaacatac accatccggg atgaaagtga gattagcccc
     3661 gagactgagg aagatggctt ccctgacgga tacctagagt gccctccacg aggtgagttt
     3721 tccgaaccca ttctggaaga ggatttcctt tttaaatcct tcgaaagctt ggaggaagtg
     3781 gagcaaaacc tttctcgcca ggttctggaa gcgagttccc ttctcgagtc ttctttggag
     3841 tacatgacaa aggggacgaa gcaagagaag acagaggtca cgcaagagac ccctccgctg
     3901 agggttgggg cgagctcatt acttgcaggc ggccctgcag agaagccaga gggcggtgtg
     3961 tactgtggtg tcttatcgat gctggagtgt cctcaagccg ggtgcaagaa gaagctgagg
     4021 ggtaaaaccg ccctgaggaa gcacatgctt gtccacgggc cccggcggca cgtgtgtgca
     4081 gagtgtggca aagccttcac ggagagctcg aaactaaagc gacattttct ggtgcacacc
     4141 ggagagaagc cgtatcagtg cacgttcgaa ggctgtggga agcgtttctc cctggatttc
     4201 aacttgcgca cccacatccg catccacacc ggggagaggc gctttgtgtg tccttttgat
     4261 ggctgcgaga agagctttat tcagtcaaat aaccagaaga ttcacatcct aacccacgca
     4321 aaggcaggga agaaatgctg atgatctaga gcccctctcc ctcccccccc ctaacgttac
     4381 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4441 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     4501 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     4561 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     4621 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     4681 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     4741 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     4801 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     4861 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata
     4921 atacctccgg aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     4981 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5041 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     5101 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     5161 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     5221 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     5281 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     5341 acggccccgt aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc
     5401 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     5461 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     5521 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5581 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggatgag ctgtacaagt
     5641 gactcgagcc ttaattaagg gctagcaaat gcatgcggcc gccaattcca gctgagcgcc
     5701 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     5761 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg
     5821 atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc
     5881 ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc
     5941 gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc
     6001 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6121 cttatcatgt ctggatctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     6181 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     6241 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     6301 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     6361 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     6421 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     6481 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     6541 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     6601 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacaagctag cttaccatga
     6661 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6721 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6781 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6841 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6901 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6961 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7021 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7081 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7141 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7201 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     7261 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatttgcc
     7321 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7381 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7441 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7501 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     7561 cgcggccggc cgctgaccaa gtcagcttgg cactggccgt cgttttacaa cgtcgtgact
     7621 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7681 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7741 gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     7801 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     7861 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     7921 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     7981 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     8041 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     8101 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     8161 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     8221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaac
//
