LOCUS       sequence-461399-       10419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of RLIM-GFP-IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward 
            DI, Bustos F
  TITLE     RLIM-specific activity reporters define variant pathogenicity in 
            Tonne-Kalscheuer syndrome.
  JOURNAL   HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378.
  PUBMED    39482882
REFERENCE   2  (bases 1 to 10419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "HGG Adv."; 
            date: "2024-10-31"; volume: "6(1):100378. doi"; pages: " 
            10.1016/j.xhgg.2024.100378"
COMMENT     Sequence Label: pCAGGS puro human-GFP-RLIM-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     979..998
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        979..997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1007..1386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1388..1663
                     /label=chicken beta-actin promoter
     intron          1664..2680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2604..2626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2688..2707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2743..3459
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    5362..5934
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5529..5546)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5756..5775
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5943..6653
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6088..6106)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    7014..7148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7051..7070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7105..7124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7153..7652
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7188..7211)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7585..7604
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7668..8267
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7668..7687)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8164..8184
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8603..8620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8603..8619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8612..8634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8760..9215)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8897..8918)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9109..9128
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9242..9346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9347..9415,9416..10207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9565..9584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10378..10419,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      961 tttggccaag tcggcctcta atacgactca ctatagggag ctcgtcgaca ttgattattg
     1021 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1201 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1261 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1321 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1381 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1441 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1501 gggggggggc gcgccaggsg gggsggggsg gggsgagggg sggggsgggg sgaggcggag
     1561 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1621 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1681 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1741 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1801 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1861 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1921 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1981 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     2041 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     2161 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     2221 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     2281 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2461 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2521 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2581 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2641 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     2701 tattgtgctg tctcatcatt ttggcaaaga attcaactcg agatggtgag caagggcgcc
     2761 gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac
     2821 aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2881 ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac caccctgagc
     2941 tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag
     3001 agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac
     3061 tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg catcgagctg
     3121 accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac
     3181 aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa ggtgaacttc
     3241 aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta ccagcagaat
     3301 acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc
     3361 gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg cttcgtgacc
     3421 gccgccgcca tcacccacgg catggatgag ctgtacaagg gaggcggagg cggatccatg
     3481 gaaaactcag attccaatga caaaggaagt ggtgatcagt ctgcagcaca gcgcagaagt
     3541 cagatggacc gattggatcg agaagaagct ttctatcaat ttgtaaataa cctgagtgaa
     3601 gaagattata ggcttatgag agataacaat ttgctaggca ccccaggtga aagtactgag
     3661 gaagagttgc tgagacgact acagcaaatt aaagaaggcc caccaccgca aaactcagat
     3721 gaaaatagag gaggagactc ttcagatgat gtgtctaatg gtgactctat aatagactgg
     3781 cttaactctg tcagacaaac tggaaataca acaagaagtg ggcaaagagg aaaccaatct
     3841 tggagagcag tgagtcggac taatccaaac agtggtgatt tcagattcag tttagagata
     3901 aatgttaacc gtaataatgg gagccaaaat tcagagaatg aaaatgagcc atctgcaaga
     3961 cgttctagtg gagaaaatgt ggaaaacaac agccaaaggc aagtggaaaa cccacgatct
     4021 gaatcaacat ctgcaaggcc atctagatca gaacgaaatt caactgaagc gttaacagag
     4081 gtcccaccta ccagaggtca gaggagggca agaagcagga gcccagacca tcggagaacc
     4141 agagcaagag ctgaaagaag taggtcacct ctgcatccaa tgagtgaaat tccacgaaga
     4201 tctcatcata gtatctcatc tcagactttt gaacatcctt tggtaaatga gacggaggga
     4261 agttctagaa cccggcacca tgtgacattg aggcagcaaa tatctgggcc tgagttgcta
     4321 agtagaggtc tttttgcagc ttctggaaca agaaatgctt ctcaaggagc aggttcttca
     4381 gacacagctg ccagtggtga atctacagga tcaggacaga gacctccaac catagtcctt
     4441 gatcttcaag taagaagagt tcgtcctgga gaatatcggc agagagatag catagccagc
     4501 agaactcggt ctaggtctca gacaccaaac aacactgtca cctatgaaag tgaacgagga
     4561 ggttttaggc gtacattttc acgttctgag cgggcaggtg tgagaaccta tgtcagtacc
     4621 atcagaattc ccattcgtag aatcttaaat actggtttaa gtgagactac atctgttgca
     4681 attcagacca tgttaaggca gataatgaca ggttttggtg agttaagcta ttttatgtac
     4741 agtgatagcg actcagagcc tactggctca gtctcaaatc gaaatatgga aagggcagag
     4801 tcacggagtg gaagaggagg ttctggtggt ggtagtagtt ctggttccag ttcgagttcc
     4861 agttccagtt cgagttccag ttccagttca agttccagtt ccagtcctag ttccagttcc
     4921 ggtggtgaaa gttcagaaac tagctcagat ttatttgaag gcagtaatga aggaagctca
     4981 tcatcaggct catcaggtgc caggcgagag ggtcgacata gggccccagt cacatttgat
     5041 gaaagtggct ctttgccctt ccttagcctg gctcagtttt tcctcttaaa tgaggatgat
     5101 gatgaccaac ctagaggact caccaaagaa cagattgaca acttggcaat gagaagtttt
     5161 ggtgaaaatg atgcattaaa aacctgtagt gtttgcatta cagaatatac agaaggcaac
     5221 aaacttcgta aactaccttg ttcccatgag taccatgtcc actgcatcga tcgctggtta
     5281 tctgagaatt ctacctgtcc tatttgtcgc agagcagtct tagcttctgg taacagagaa
     5341 agtgttgtgt aaggatccta gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     5401 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5461 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5521 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5581 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5641 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5701 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     5761 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     5821 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     5881 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aatacctccg
     5941 gaatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6001 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6061 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6121 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     6181 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     6241 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     6301 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6361 taatgcagaa gaaaaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     6421 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     6481 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     6541 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6601 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tgactcgagc
     6661 cttaattaag ggctagcaaa tgcatgcggc cgccaattcc agctgagcgc cggtcgctac
     6721 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
     6781 tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
     6841 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
     6901 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
     6961 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggaacttgt
     7021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7141 tctggatcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     7201 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     7261 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     7321 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     7381 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     7441 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     7501 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     7561 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     7621 tgttctcctc ttcctcatct ccgggccttt cgacaagcta gcttaccatg accgagtaca
     7681 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7741 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     7801 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7861 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7921 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7981 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8041 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8101 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8161 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8221 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     8281 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatttgc cagccatctg
     8341 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     8401 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8461 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8521 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg acgcggccgg
     8581 ccgctgacca agtcagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8641 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8701 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     8761 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8821 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8881 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8941 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9001 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9061 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9121 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9181 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     9241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9361 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9421 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9481 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9541 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9601 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9661 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9721 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9781 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9841 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9901 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9961 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    10021 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    10081 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    10141 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10201 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10381 agatcctttt tttctgcgcg taatctgctg cttgcaaac
//
