LOCUS       sequence-461400-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of REX1-GFP-IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward 
            DI, Bustos F
  TITLE     RLIM-specific activity reporters define variant pathogenicity in 
            Tonne-Kalscheuer syndrome.
  JOURNAL   HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378.
  PUBMED    39482882
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "HGG Adv."; 
            date: "2024-10-31"; volume: "6(1):100378. doi"; pages: " 
            10.1016/j.xhgg.2024.100378"
COMMENT     Sequence Label: pCAGGS puro GFP-REX1-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     979..998
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        979..997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1007..1386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1388..1663
                     /label=chicken beta-actin promoter
     intron          1664..2680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2604..2626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2688..2707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2743..3459
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    4352..4924
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4519..4536)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4746..4765
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4933..5643
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5078..5096)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    6004..6138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6041..6060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6095..6114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6143..6642
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6178..6201)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6575..6594
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6658..7257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6658..6677)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7154..7174
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7593..7610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7593..7609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7602..7624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7750..8205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7887..7908)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8099..8118
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8232..8336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8337..8405,8406..9197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8555..8574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9368..9409,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      961 tttggccaag tcggcctcta atacgactca ctatagggag ctcgtcgaca ttgattattg
     1021 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1201 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1261 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1321 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1381 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1441 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1501 gggggggsgc gcgccaggsg gggsggggsg gggsgagggg sggggsgggg sgaggcggag
     1561 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1621 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1681 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1741 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1801 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1861 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1921 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1981 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     2041 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     2161 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     2221 gccgggcggg gggtggcggc aggkgggggt gccgggcggg gcggggccgc ckcgggccgg
     2281 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2461 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2521 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2581 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2641 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     2701 tattgtgctg tctcatcatt ttggcaaaga attcaactcg agatggtgag caagggcgcc
     2761 gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac
     2821 aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2881 ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac caccctgagc
     2941 tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag
     3001 agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac
     3061 tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg catcgagctg
     3121 accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac
     3181 aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa ggtgaacttc
     3241 aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta ccagcagaat
     3301 acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc
     3361 gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg cttcgtgacc
     3421 gccgccgcca tcacccacgg catggatgag ctgtacaagg gaggcggagg cagcatgaat
     3481 gaacaaaaaa tgaatgaaca aatgaagaaa acggcaaaga caagtggcca gaaagggccg
     3541 ggcggaagag ccctcgacag actgacccta aagcaagacg aggcaaggcc agtccagaat
     3601 accagagtgg aagctccccg tgtaacatac accatccggg atgaaagtga gattagcccc
     3661 gagactgagg aagatggctt ccctgacgga tacctagagt gcatcatacg aggtgagttt
     3721 tccgaaccca ttctggaaga ggatttcctt tttaaatcct tcgaaagctt ggaggaagtg
     3781 gagcaaaacc tttctcgcca ggttctggaa gcgagttccc ttctcgagtc ttctttggag
     3841 tacatgacaa aggggacgaa gcaagagaag acagaggtca cgcaagagac ccctccgctg
     3901 agggttgggg cgagctcatt acttgcaggc ggccctgcag agaagccaga gggcggtgtg
     3961 tactgtggtg tcttatcgat gctggagtgt cctcaagccg ggtgcaagaa gaagctgagg
     4021 ggtaaaaccg ccctgaggaa gcacatgctt gtccacgggc cccggcggca cgtgtgtgca
     4081 gagtgtggca aagccttcac ggagagctcg aaactaaagc gacattttct ggtgcacacc
     4141 ggagagaagc cgtatcagtg cacgttcgaa ggctgtggga agcgtttctc cctggatttc
     4201 aacttgcgca cccacatccg catccacacc ggggagaggc gctttgtgtg tccttttgat
     4261 ggctgcgaga agagctttat tcagtcaaat aaccagaaga ttcacatcct aacccacgca
     4321 aaggcaggga agaaatgctg atgatctaga gcccctctcc ctcccccccc cctaacgtta
     4381 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4441 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4501 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4561 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4621 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4681 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4741 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4801 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4861 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4921 aatacctccg gaatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     4981 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5041 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5101 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5161 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5221 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5281 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5341 gacggccccg taatgcagaa gaaaaccatg ggctgggagg cctcctccga gcggatgtac
     5401 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5461 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5521 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5581 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5641 tgactcgagc cttaattaag ggctagcaaa tgcatgcggc cgccaattcc agctgagcgc
     5701 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     5761 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
     5821 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt
     5881 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
     5941 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
     6001 cggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6121 tcttatcatg tctggatcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     6181 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     6241 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     6301 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     6361 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     6421 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     6481 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     6541 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     6601 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacaagcta gcttaccatg
     6661 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6721 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6781 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6841 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6901 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6961 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7021 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7081 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7141 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7201 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     7261 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatttgc
     7321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7381 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7441 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7501 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     7561 acgcggccgg ccgctgacca agtcagcttg gcactggccg tcgttttaca acgtcgtgac
     7621 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7681 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7741 ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7801 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7861 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7921 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7981 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8041 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8101 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8161 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     8221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaac
//
