LOCUS       sequence-461402-       10707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentivirus-mediated co-expression of GFP and FOXP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Shin D, Kim CN, Ross J, Hennick KM, Wu SR, Paranjape N, Leonard R, 
            Wang JC, Keefe MG, Pavlovic BJ, Donohue KC, Moreau C, Wigdor EM, 
            Larson HH, Allen DE, Cadwell CR, Bhaduri A, Popova G, Bearden CE, 
            Pollen AA, Jacquemont S, Sanders SJ, Haussler D, Wiita AP, Frost NA,
            Sohal VS, Nowakowski TJ
  TITLE     Thalamocortical organoids enable in vitro modeling of 22q11.2 
            microdeletion associated with neuropsychiatric disorders.
  JOURNAL   Cell Stem Cell. 2024 Mar 7;31(3):421-432.e8. doi: 
            10.1016/j.stem.2024.01.010. Epub 2024 Feb 20.
  PUBMED    38382530
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2024.01.010"; journalName: "Cell Stem Cell"; date: 
            "2024-03-7- 7"; volume: "31"; issue: "3"; pages: "421-432.e8"
COMMENT     Sequence Label: pSicoFlipped-CAG-GFP-2A-FOXP2-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(949..1147)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(974..994)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1149..1528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1700..1719
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1899..1967,1968..2759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3580..3760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4169..4548
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4550..4825
                     /label=chicken beta-actin promoter
     intron          4826..5842
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5766..5788
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      5874..5883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5880..5882,5883..5885,5886..6596)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5880..5882,5883..5885,5886..6596)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5925..5946)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6186..6205)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6533..6554
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6606..6659
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8919..9507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8972..8992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9390..9401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9630..9854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    9912..10029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10327..10371)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10556..10707,1..82)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
       61 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
      121 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
      181 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
      241 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      301 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      361 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      421 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      481 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      541 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      601 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      661 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      721 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      781 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      841 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      901 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
      961 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     1021 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1081 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1141 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1201 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1261 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1321 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1381 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1441 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1501 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     1561 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1621 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1681 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     1741 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgct gctagagatt ttccacactg
     3601 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3661 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3721 agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct
     3781 ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct
     3841 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat
     3901 tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca
     3961 gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg
     4021 tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca
     4081 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
     4141 ttaaaggtac tacatcgtag atcagctcga cattgattat tgactagtta ttaatagtaa
     4201 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     4261 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4321 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4381 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4441 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4501 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     4561 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     4621 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc gcgcgccagg
     4681 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     4741 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     4801 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     4861 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     4921 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     4981 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     5041 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     5101 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     5161 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     5221 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     5281 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     5341 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     5401 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     5461 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     5521 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     5581 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     5641 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     5701 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     5761 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     5821 tgccttcttc tttttcctac agctcctgct agtcgtaact agcgctaccg gtcgccacca
     5881 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5941 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6001 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6061 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     6121 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6181 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6241 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6301 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     6361 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     6421 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6481 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     6541 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggggt
     6601 ccggggaagg gcgcggttcc ttgcttacgt gtggtgacgt ggaggagaat cctgggcctg
     6661 aattcatgca ggaatctgcg acagagacaa taagcaacag ttcaatgaat caaaatggaa
     6721 tgagcactct aagcagccaa ttagatgctg gcagcagaga tggaagatca agtggtgaca
     6781 ccagctctga agtaagcaca gtagaactgc tgcatctgca acaacagcag gctctccagg
     6841 cagcaagaca acttctttta cagcagcaaa caagtggatt gaaatctcct aagagcagtg
     6901 ataaacagag accactgcag gaattgcttc cagaaacaaa attatgtatc tgtggccact
     6961 cttctggtga tgggcatcct cacaacacat ttgcagtgcc tgtgtcagtg gccatgatga
     7021 ctccccaggt gatcacccct cagcaaatgc agcagatcct tcagcaacaa gtcctgtctc
     7081 ctcagcagct acaagccctt ctccaacaac agcaggctgt catgctgcag cagcaacaac
     7141 tacaagagtt ttacaagaaa cagcaagagc agttacatct tcagcttttg cagcagcagc
     7201 agcaacagca gcagcagcaa caacagcagc aacaacagca gcagcaacaa caacaacaac
     7261 agcagcaaca acagcagcag cagcagcaac agcagcagca gcagcaacag catcctggaa
     7321 agcaagcgaa agagcagcag cagcagcagc agcagcaaca gcaattggca gcccagcagc
     7381 ttgtcttcca gcagcagctt ctccagatgc aacaactcca gcagcagcag catctgctca
     7441 gccttcagcg tcagggactc atctccattc cacctggcca ggcagcactt cctgtccaat
     7501 cgctgcctca agctggctta agtcctgctg agattcagca gttatggaaa gaagtgactg
     7561 gagttcacag tatggaagac aatggcatta aacatggagg gctagacctc actactaaca
     7621 attcctcctc gactacctcc tccaacactt ccaaagcatc accaccaata actcatcatt
     7681 ccatagtgaa tggacagtct tcagttctaa gtgcaagacg agacagctcg tcacatgagg
     7741 agactggggc ctctcacact ctctatggcc atggagtttg caaatggcca ggctgtgaaa
     7801 gcatttgtga agattttgga cagtttttaa agcaccttaa caatgaacac gcattggatg
     7861 accgaagcac tgctcagtgt cgagtgcaaa tgcaggtggt gcaacagtta gaaatacagc
     7921 tttctaaaga acgcgaacgt cttcaagcaa tgatgaccca cttgcacatg cgaccctcag
     7981 agcccaaacc atctcccaaa cctctaaatc tggtgtctag tgtcaccatg tcgaagaata
     8041 tgttggagac atccccacag agcttacctc aaacccctac cacaccaacg gccccagtca
     8101 ccccgattac ccagggaccc tcagtaatca ccccagccag tgtgcccaat gtgggagcca
     8161 tacgaaggcg acattcagac aaatacaaca ttcccatgtc atcagaaatt gccccaaact
     8221 atgaatttta taaaaatgca gatgtcagac ctccatttac ttatgcaact ctcataaggc
     8281 aggctatcat ggagtcatct gacaggcagt taacacttaa tgaaatttac agctggttta
     8341 cacggacatt tgcttacttc aggcgtaatg cagcaacttg gaagaatgca gtacgtcata
     8401 atcttagcct gcacaagtgt tttgttcgag tagaaaatgt taaaggagca gtatggactg
     8461 tggatgaagt agaataccag aagcgaaggt cacaaaagat aacaggaagt ccaaccttag
     8521 taaaaaatat acctaccagt ttaggctatg gagcagctct taatgccagt ttgcaggctg
     8581 ccttggcaga gagcagttta cctttgctaa gtaatcctgg actgataaat aatgcatcca
     8641 gtggcctact gcaggccgtc cacgaagacc tcaatggttc tctggatcac attgacagca
     8701 atggaaacag tagtccgggc tgctcacctc agccgcacat acattcaatc cacgtcaagg
     8761 aagagccagt gattgcagag gatgaagact gcccaatgtc cttagtgaca acagctaatc
     8821 acagtccaga attagaagac gacagagaga ttgaagaaga gcctttatct gaagatctgg
     8881 aatgaggccg ctacaaagct tatgccgacg tacgatccaa tcaacctctg gattacaaaa
     8941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9061 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9121 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9181 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9241 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9301 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9361 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9421 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9481 ggatctccct ttgggccgcc tccccgcgtc ctggacctcc ctagcaaact ggggcacaag
     9541 cgaattcatt ccgtgagctc acggattcat tccgtgagct cacggatcgt gccaacatgg
     9601 tgagctagcc gcacacgtgt ggactcgagc tgtgccttct agttgccagc catctgttgt
     9661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9721 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9781 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     9841 ggtgggctct atggctagag cgggcccggt actaaccaaa ctggatctct gctgtccctg
     9901 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     9961 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
    10021 cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga
    10081 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
    10141 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
    10201 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
    10261 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
    10321 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
    10381 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
    10441 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
    10501 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
    10561 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
    10621 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
    10681 ctgctgcact ataccagaca ataattg
//
