LOCUS       sequence-461403-        9379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward 
            DI, Bustos F
  TITLE     RLIM-specific activity reporters define variant pathogenicity in 
            Tonne-Kalscheuer syndrome.
  JOURNAL   HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378.
  PUBMED    39482882
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "HGG Adv."; 
            date: "2024-10-31"; volume: "6(1):100378. doi"; pages: " 
            10.1016/j.xhgg.2024.100378"
COMMENT     Sequence Label: pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     979..998
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        979..997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1007..1386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1388..1663
                     /label=chicken beta-actin promoter
     intron          1664..2680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2604..2626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2688..2707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2743..3459
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    4322..4894
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4489..4506)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4716..4735
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4903..5613
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5048..5066)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    5974..6108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6011..6030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6065..6084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6113..6612
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6148..6171)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6545..6564
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6628..7227
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6628..6647)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7124..7144
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7563..7580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7563..7579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7572..7594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7720..8175)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7857..7878)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8069..8088
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8202..8306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8307..8375,8376..9167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8525..8544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9338..9379,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      961 tttggccaag tcggcctcta atacgactca ctatagggag ctcgtcgaca ttgattattg
     1021 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1201 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1261 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1321 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1381 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     1441 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     1501 gggggggsgc gcgccaggsg gggggggggg gggsgagggg sggggsgggg sgaggcggag
     1561 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1621 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1681 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1741 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1801 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1861 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1921 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1981 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     2041 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     2161 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     2221 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     2281 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2461 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2521 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2581 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2641 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     2701 tattgtgctg tctcatcatt ttggcaaaga attcaactcg agatggtgag caagggcgcc
     2761 gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac
     2821 aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2881 ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac caccctgagc
     2941 tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag
     3001 agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac
     3061 tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg catcgagctg
     3121 accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac
     3181 aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa ggtgaacttc
     3241 aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta ccagcagaat
     3301 acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc
     3361 gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg cttcgtgacc
     3421 gccgccgcca tcacccacgg catggatgag ctgtacaagg gaggcggagg cagcatgaat
     3481 gaacaaaaaa tgaatgaaca aatgaagaaa acggcaaaga caagtggcca gaaagggccg
     3541 ggcggaagag ccctcgacag actgacccta aagcaagacg aggcaaggcc agtccagaat
     3601 accagagtgg aagctccccg tgtaacatac accatccggg atgaaagtga gattagcccc
     3661 gagactgagg aagatggctt ccctgacgga tccgaaccca ttctggaaga ggatttcctt
     3721 tttaaatcct tcgaaagctt ggaggaagtg gagcaaaacc tttctcgcca ggttctggaa
     3781 gcgagttccc ttctcgagtc ttctttggag tacatgacaa aggggacgaa gcaagagaag
     3841 acagaggtca cgcaagagac ccctccgctg agggttgggg cgagctcatt acttgcaggc
     3901 ggccctgcag agaagccaga gggcggtgtg tactgtggtg tcttatcgat gctggagtgt
     3961 cctcaagccg ggtgcaagaa gaagctgagg ggtaaaaccg ccctgaggaa gcacatgctt
     4021 gtccacgggc cccggcggca cgtgtgtgca gagtgtggca aagccttcac ggagagctcg
     4081 aaactaaagc gacattttct ggtgcacacc ggagagaagc cgtatcagtg cacgttcgaa
     4141 ggctgtggga agcgtttctc cctggatttc aacttgcgca cccacatccg catccacacc
     4201 ggggagaggc gctttgtgtg tccttttgat ggctgcgaga agagctttat tcagtcaaat
     4261 aaccagaaga ttcacatcct aacccacgca aaggcaggga agaaatgctg atgatctaga
     4321 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4381 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4441 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4501 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4561 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4621 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4681 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4741 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4801 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     4861 gggacgtggt tttcctttga aaaacacgat aatacctccg gaatggtgag caagggcgag
     4921 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     4981 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5041 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     5101 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     5161 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5221 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     5281 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaaaaccatg
     5341 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     5401 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     5461 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     5521 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     5581 tccaccggcg gcatggacga gctgtacaag tgactcgagc cttaattaag ggctagcaaa
     5641 tgcatgcggc cgccaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
     5701 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
     5761 tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt
     5821 ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat
     5881 cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca
     5941 accgctgttt ggtctgcttt ctgacaaact cggaacttgt ttattgcagc ttataatggt
     6001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcta ccgggtaggg
     6121 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     6181 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     6241 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     6301 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     6361 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     6421 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     6481 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     6541 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     6601 ccgggccttt cgacaagcta gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6661 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6721 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6781 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6841 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6901 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6961 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7021 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7081 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7141 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7201 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     7261 aggagcgcac gaccccatgc atcgatttgc cagccatctg ttgtttgccc ctcccccgtg
     7321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7501 tctgaggcgg aaagaaccag ctggggctcg acgcggccgg ccgctgacca agtcagcttg
     7561 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7621 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7681 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt
     7741 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7801 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7861 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7921 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     7981 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8041 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8101 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8161 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9361 taatctgctg cttgcaaac
//
