LOCUS       sequence-461413-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD 
            (186-320) with all aromatic amino acids mutated to W keeping the 
            hexapeptide fibril core (SYNDFG) intact..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, 
            Mittag T
  TITLE     Metastable condensates suppress conversion to amyloid fibrils.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569.
  PUBMED    38464104
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569."
COMMENT     Sequence Label: A1-LCD allW
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     156..175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        156..174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             206..228
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             248..265
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    275..299
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             302..322
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(738..758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
       61 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
      121 cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta tagggagacc
      181 acaacggttt ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgtc
      241 gtactaccat caccatcacc atcacctcga atcaacaagt ttgtacaaaa aagcaggctt
      301 cgagaacctg tattttcagg gtagcatggc aagcgcaagc agcagccagc gtggtcgtag
      361 cggtagcggt aattggggtg gtggtcgcgg tggtggttgg ggtggcaatg ataactgggg
      421 tcgtggtggt aattggagcg gtcgcggagg ctggggtggt tcaagaggtg gtggcggatg
      481 gggtggtagt ggtgatggtt ggaatggttg gggcaatgat ggtagcaact ggggtggcgg
      541 tggcagctat aatgactttg gcaattggaa taatcagagc agcaattggg gtccgatgaa
      601 aggtggtaac tggggaggtc gtagcagcgg tccgtgggga ggtggcggtc agtggtgggc
      661 aaaaccgcgt aatcaaggcg gttggggagg tagctctagc agcagcagct ggggttcagg
      721 tcgtcgttgg taagacccag ctttcttgta caaagtgg
//
