LOCUS       sequence-461419-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD 
            (186-320) D262N with all aromatic amino acids mutated to W keeping 
            the hexapeptide fibril core (SYNDFG) intact..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, 
            Mittag T
  TITLE     Metastable condensates suppress conversion to amyloid fibrils.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569.
  PUBMED    38464104
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569."
COMMENT     Sequence Label: A1-LCD allW D262N
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             222..242
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(655..679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
       61 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata tacatatgtc gtactaccat caccatcacc
      181 atcacctcga atcaacaagt ttgtacaaaa aagcaggctt cgagaacctg tattttcagg
      241 gtagcatggc aagcgcaagc agcagccagc gtggtcgtag cggtagcggt aattggggtg
      301 gtggtcgcgg tggtggttgg ggtggcaatg ataactgggg tcgtggtggt aattggagcg
      361 gtcgcggagg ctggggtggt tcaagaggtg gtggcggatg gggtggtagt ggtgatggtt
      421 ggaatggttg gggcaatgat ggtagcaact ggggtggcgg tggcagctat aataactttg
      481 gcaattggaa taatcagagc agcaattggg gtccgatgaa aggtggtaac tggggaggtc
      541 gtagcagcgg tccgtgggga ggtggcggtc agtggtgggc aaaaccgcgt aatcaaggcg
      601 gttggggagg tagctctagc agcagcagct ggggttcagg tcgtcgttgg taagacccag
      661 ctttcttgta caaagtggtt gattcgaggc tgctaacaaa gcccg
//
