LOCUS       sequence-46148-e       13678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1338
FEATURES             Location/Qualifiers
     source          1..13678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1308..1317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1314..1316,1317..1319,1320..2033)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1314..1316,1317..1319,1320..2030)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1359..1380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1620..1639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1967..1988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2074..2662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2127..2147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2545..2556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2665..2681)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2666..2682)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3187..3367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3393..3410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3399..3623
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3669..4097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3756..3775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3966..3987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4106..4126)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4111..4440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4291..4426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4353..4372
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4450..5049
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4450..4469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4946..4966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5110..5478
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     polyA_signal    5608..5729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5645..5664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5699..5718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5778..5794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5778..5794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5791..5813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5802..5818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5826..5832,5833..5850,5851..5856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5871..5892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6009..6026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6180..6768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6260..6279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6939..7730,7731..7799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7562..7581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7800..7904)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7972..7990
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8028..8050)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8150..8169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8186..9350
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9656..9689
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(9755..9952)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(10762..11105)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    11173..11206
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        11311..11690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11692..11890
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11845..11865
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11908..12088
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12135..12260
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12753..12986
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13171..13215
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13513..13630
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(13675..13678,1..1286)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
ORIGIN
        1 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
       61 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
      121 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
      181 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
      241 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
      301 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
      361 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
      421 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
      481 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
      541 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
      601 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
      661 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
      721 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
      781 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
      841 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
      901 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc
      961 ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca cacagtcctg
     1021 cagtattgtg tatataaggc cagggcaaag gaggagcagg ttttaaagtg aaaggcaggc
     1081 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
     1141 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
     1201 gaagccccaa gctcgtcagt caagccggtt ctccgtttgc actcaggagc acgggcaggc
     1261 gagtggcccc tagttctggg ggcagcgggg atccccgggt accggtcgcc accatggtga
     1321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1501 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1741 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1801 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1921 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     2041 gcgactctag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2461 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2641 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     2701 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     2761 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     2821 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     2881 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     2941 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     3001 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     3061 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     3121 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     3181 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3361 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3421 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3481 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3541 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3601 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3661 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3841 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3901 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3961 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4021 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4081 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4201 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4261 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4321 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4381 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4441 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4501 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4561 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4621 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     4681 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     4741 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     4801 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     4861 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     4921 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     4981 gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc
     5041 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     5101 gccagtccca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5161 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5221 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     5281 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     5341 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     5401 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     5461 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     5521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7921 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7981 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8041 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8101 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     8161 cagattgtac tgagagtgca ccataacgca tttaagcata aacacgcact atgccgttct
     8221 tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
     8281 gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
     8341 tcctattccg aagttcctat tctgatccta gaaagtatag gaacttcaga gcgcttttga
     8401 aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg
     8461 agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct
     8521 atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt
     8581 gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa
     8641 aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt
     8701 tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg
     8761 aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag
     8821 aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag
     8881 taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc
     8941 cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag
     9001 cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga
     9061 tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc
     9121 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     9181 cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct
     9241 ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     9301 gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata
     9361 tgctattgaa gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt
     9421 acctatgcaa tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta
     9481 aagcgctaga ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact
     9541 cccaggttcg gatgttcaaa attcaatgat gggtaacaag tagcttttca attcaattca
     9601 tcattttttt tttattcttt ttttcttcgt acgctgcagg tcgacaaccc ttaatataac
     9661 ttcgtataat gtatgctata cgaagttatt aggtctagag atctgaattc gagctcgttt
     9721 aaactggatg gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac
     9781 gattgacgca tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga
     9841 ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa
     9901 aaaactatac aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtacctatta
     9961 atggggagcg ctgattctct tttggtacgc ttcccatcca gcatttctgt atctttcacc
    10021 ttcaacctta ggatctctac ccttggcgaa aagtcctctg ccaacaatga tgatatctga
    10081 tccaccactt acaacttcgt cgacggttct gtactgctga cccaatgcat cgcctttgtc
    10141 gtctaaacct acacctgggg tcatgattag ccaatcaaac ccttcttctc ttcctcccat
    10201 atcgttctga gcaatgaacc caataacgaa atctttatca ctctttgcaa tatcaacggt
    10261 acccttagta tattcaccgt gtgctagaga acccttggaa gacaattcag caagcatcaa
    10321 taatcccctt ggttctttgg tgacctcttg cgcaccttgt ttcaagccag caacaatacc
    10381 agcaccagta accccgtggg cgttggtgat atcagaccat tctgcgatac ggtaaacgcc
    10441 cgatgtatat tgtaatttga ctgtgttacc gatatcggcg aattttctgt cctcaaatat
    10501 caagaacttg tatttctctg ccaatgcttt caatggaacg acagtaccct cataactgaa
    10561 atcatccaag atatcaacgt gtgttttcaa aaggcaaatg tatggaccca acgtttcaac
    10621 aagtttcaat agctcatcag tcgaacgaac gtcaagggaa gcacacaaat tggtcttctt
    10681 ttcatccatt aaacgtaaaa gtttcgatgc aaccggactt gcatgagtct cagctctact
    10741 ggtatatgat tttgtggaca tggttgttta tgttcggatg tgatgtgaga actgtatcct
    10801 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
    10861 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
    10921 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
    10981 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
    11041 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
    11101 atgtcgctgg ccgggtgacc cggcggggac gaggcaagct aaacagatcc aagcttctcg
    11161 agaaccctta atataacttc gtataatgta tgctatacga agttattagg tgatatcaga
    11221 tccactagtg gcctatgcat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    11281 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
    11341 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    11401 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    11461 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    11521 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    11581 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    11641 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    11701 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    11761 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    11821 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    11881 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    11941 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    12001 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    12061 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    12121 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    12181 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    12241 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    12301 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    12361 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    12421 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    12481 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    12541 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    12601 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
    12661 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    12721 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    12781 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    12841 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    12901 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    12961 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    13021 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    13081 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    13141 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    13201 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    13261 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    13321 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    13381 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    13441 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
    13501 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    13561 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    13621 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaacatt
//
