LOCUS       sequence-46149-e       12640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1260
FEATURES             Location/Qualifiers
     source          1..12640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(782..1585)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILIDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(1586..1801)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      2032..3196
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     complement(3213..3232)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3332..3354
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3392..3410)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3478..3582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3583..3651,3652..4443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3801..3820)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4614..5202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5103..5122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatcccctg aaaaggctgt ttaagttggg taaaccgctc ccagccgacg acgagcaaga
       61 cgaagacaga agacgcgctc tgctagatga aacaaaggcg tggtttagag taggtataac
      121 aggcacttta gcagtggccg tgacgacccg gtatgaggta gacaatatta cacctgtcct
      181 actggcattg agaacttttg cccagagcaa aagagcattc caagccatca gaggggaaat
      241 aaagcatctc tacggtggtc ctaaatagtc agcatagtac atttcatctg actaatacta
      301 caacaccacc acctctagac gcgtagatct cacgtgagca tgcaggcctt gggcccaatg
      361 atccgaccag caaaactcga tgtacttccg aggaactgat gtgcataatg catcaggctg
      421 gtacattaga tccccgctta ccgcgggcaa tatagcaaca ctaaaaactc gatgtacttc
      481 cgaggaagcg cagtgcataa tgctgcgcag tgttgccaca taaccactat attaaccatt
      541 tatctagcgg acgccaaaaa ctcaatgtat ttctgaggaa gcgtggtgca taatgccacg
      601 cagcgtctgc ataactttta ttatttcttt tattaatcaa caaaattttg tttttaacat
      661 ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagggga attcctcgat taattaagcg
      721 gccgctcgag agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt
      781 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
      841 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
      901 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
      961 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     1021 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     1081 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca
     1141 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa
     1201 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     1261 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     1321 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     1381 aaaaatcaat caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     1441 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     1501 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     1561 gttccttata tgtagctttc gacatgattt atcttcgttt cggtttttgt tctgtgcagt
     1621 tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc
     1681 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
     1741 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc
     1801 acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat
     1861 agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt
     1921 atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc
     1981 ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca
     2041 tctgtgcttc attttgtaga acaaaaaagc aacgcgagag cgctaatttt tcaaacaaag
     2101 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     2161 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     2221 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     2281 aacaaagaat ctatacttcc tttttgttct acaaaaatgc atcccgagag cgctattttt
     2341 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     2401 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctatct
     2461 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     2521 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     2581 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     2641 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     2701 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     2761 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     2821 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     2881 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     2941 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     3001 cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     3061 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     3121 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     3181 ttatgcttaa atgcgttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3241 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3301 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3361 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3421 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     3841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4741 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4981 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     5041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcgagc
     5221 tcgtatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     5281 acacatacga tttaggggac actatagatt gacggcgtag tacacactat tgaatcaaac
     5341 agccgaccaa ttgcactacc atcacaatgg agaagccagt agtaaacgta gacgtagacc
     5401 cccagagtcc gtttgtcgtg caactgcaaa aaagcttccc gcaatttgag gtagtagcac
     5461 agcaggtcac tccaaatgac catgctaatg ccagagcatt ttcgcatctg gccagtaaac
     5521 taatcgagct ggaggttcct accacagcga cgatcttgga cataggcagc gcaccggctc
     5581 gtagaatgtt ttccgagcac cagtatcatt gtgtctgccc catgcgtagt ccagaagacc
     5641 cggaccgcat gatgaaatac gccagtaaac tggcggaaaa agcgtgcaag attacaaaca
     5701 agaacttgca tgagaagatt aaggatctcc ggaccgtact tgatacgccg gatgctgaaa
     5761 caccatcgct ctgctttcac aacgatgtta cctgcaacat gcgtgccgaa tattccgtca
     5821 tgcaggacgt gtatatcaac gctcccggaa ctatctatca tcaggctatg aaaggcgtgc
     5881 ggaccctgta ctggattggc ttcgacacca cccagttcat gttctcggct atggcaggtt
     5941 cgtaccctgc gtacaacacc aactgggccg acgagaaagt ccttgaagcg cgtaacatcg
     6001 gactttgcag cacaaagctg agtgaaggta ggacaggaaa attgtcgata atgaggaaga
     6061 aggagttgaa gcccgggtcg cgggtttatt tctccgtagg atcgacactt tatccagaac
     6121 acagagccag cttgcagagc tggcatcttc catcggtgtt ccacttgaat ggaaagcagt
     6181 cgtacacttg ccgctgtgat acagtggtga gttgcgaagg ctacgtagtg aagaaaatca
     6241 ccatcagtcc cgggatcacg ggagaaaccg tgggatacgc ggttacacac aatagcgagg
     6301 gcttcttgct atgcaaagtt actgacacag taaaaggaga acgggtatcg ttccctgtgt
     6361 gcacgtacat cccggccacc atatgcgatc agatgactgg tataatggcc acggatatat
     6421 cacctgacga tgcacaaaaa cttctggttg ggctcaacca gcgaattgtc attaacggta
     6481 ggactaacag gaacaccaac accatgcaaa attaccttct gccgatcata gcacaagggt
     6541 tcagcaaatg ggctaaggag cgcaaggatg atcttgataa cgagaaaatg ctgggtacta
     6601 gagaacgcaa gcttacgtat ggctgcttgt gggcgtttcg cactaagaaa gtacattcgt
     6661 tttatcgccc acctggaacg cagacctgcg taaaagtccc agcctctttt agcgcttttc
     6721 ccatgtcgtc cgtatggacg acctctttgc ccatgtcgct gaggcagaaa ttgaaactgg
     6781 cattgcaacc aaagaaggag gaaaaactgc tgcaggtctc ggaggaatta gtcatggagg
     6841 ccaaggctgc ttttgaggat gctcaggagg aagccagagc ggagaagctc cgagaagcac
     6901 ttccaccatt agtggcagac aaaggcatcg aggcagccgc agaagttgtc tgcgaagtgg
     6961 aggggctcca ggcggacatc ggagcagcat tagttgaaac cccgcgcggt cacgtaagga
     7021 taatacctca agcaaatgac cgtatgatcg gacagtatat cgttgtctcg ccaaactctg
     7081 tgctgaagaa tgccaaactc gcaccagcgc acccgctagc agatcaggtt aagatcataa
     7141 cacactccgg aagatcagga aggtacgcgg tcgaaccata cgacgctaaa gtactgatgc
     7201 cagcaggagg tgccgtacca tggccagaat tcctagcact gagtgagagc gccacgttag
     7261 tgtacaacga aagagagttt gtgaaccgca aactatacca cattgccatg catggccccg
     7321 ccaagaatac agaagaggag cagtacaagg ttacaaaggc agagcttgca gaaacagagt
     7381 acgtgtttga cgtggacaag aagcgttgcg ttaagaagga agaagcctca ggtctggtcc
     7441 tctcgggaga actgaccaac cctccctatc atgagctagc tctggaggga ctgaagaccc
     7501 gacctgcggt cccgtacaag gtcgaaacaa taggagtgat aggcacaccg gggtcgggca
     7561 agtcagctat tatcaagtca actgtcacgg cacgagatct tgttaccagc ggaaagaaag
     7621 aaaattgtcg cgaaattgag gccgacgtgc taagactgag gggtatgcag attacgtcga
     7681 agacagtaga ttcggttatg ctcaacggat gccacaaagc cgtagaagtg ctgtacgttg
     7741 acgaagcgtt cgcgtgccac gcaggagcac tacttgcctt gattgctatc gtcaggcccc
     7801 gcaagaaggt agtactatgc ggagacccca tgcaatgcgg attcttcaac atgatgcaac
     7861 taaaggtaca tttcaatcac cctgaaaaag acatatgcac caagacattc tacaagtata
     7921 tctcccggcg ttgcacacag ccagttacag ctattgtatc gacactgcat tacgatggaa
     7981 agatgaaaac cacgaacccg tgcaagaaga acattgaaat cgatattaca ggggccacaa
     8041 agccgaagcc aggggatatc atcctgacat gtttccgcgg gtgggttaag caattgcaaa
     8101 tcgactatcc cggacatgaa gtaatgacag ccgcggcctc acaagggcta accagaaaag
     8161 gagtgtatgc cgtccggcaa aaagtcaatg aaaacccact gtacgcgatc acatcagagc
     8221 atgtgaacgt gttgctcacc cgcactgagg acaggctagt gtggaaaacc ttgcagggcg
     8281 acccatggat taagcagccc actaacatac ctaaaggaaa ctttcaggct actatagagg
     8341 actgggaagc tgaacacaag ggaataattg ctgcaataaa cagccccact ccccgtgcca
     8401 atccgttcag ctgcaagacc aacgtttgct gggcgaaagc attggaaccg atactagcca
     8461 cggccggtat cgtacttacc ggttgccagt ggagcgaact gttcccacag tttgcggatg
     8521 acaaaccaca ttcggccatt tacgccttag acgtaatttg cattaagttt ttcggcatgg
     8581 acttgacaag cggactgttt tctaaacaga gcatcccact aacgtaccat cccgccgatt
     8641 cagcgaggcc ggtagctcat tgggacaaca gcccaggaac ccgcaagtat gggtacgatc
     8701 acgccattgc cgccgaactc tcccgtagat ttccggtgtt ccagctagct gggaagggca
     8761 cacaacttga tttgcagacg gggagaacca gagttatctc tgcacagcat aacctggtcc
     8821 cggtgaaccg caatcttcct cacgccttag tccccgagta caaggagaag caacccggcc
     8881 cggtcaaaaa attcttgaac cagttcaaac accactcagt acttgtggta tcagaggaaa
     8941 aaattgaagc tccccgtaag agaatcgaat ggatcgcccc gattggcata gccggtgcag
     9001 ataagaacta caacctggct ttcgggtttc cgccgcaggc acggtacgac ctggtgttca
     9061 tcaacattgg aactaaatac agaaaccacc actttcagca gtgcgaagac catgcggcga
     9121 ccttaaaaac cctttcgcgt tcggccctga attgccttaa cccaggaggc accctcgtgg
     9181 tgaagtccta tggctacgcc gaccgcaaca gtgaggacgt agtcaccgct cttgccagaa
     9241 agtttgtcag ggtgtctgca gcgagaccag attgtgtctc aagcaataca gaaatgtacc
     9301 tgattttccg acaactagac aacagccgta cacggcaatt caccccgcac catctgaatt
     9361 gcgtgatttc gtccgtgtat gagggtacaa gagatggagt tggagccgcg ccgtcatacc
     9421 gcaccaaaag ggagaatatt gctgactgtc aagaggaagc agttgtcaac gcagccaatc
     9481 cgctgggtag accaggcgaa ggagtctgcc gtgccatcta taaacgttgg ccgaccagtt
     9541 ttaccgattc agccacggag acaggcaccg caagaatgac tgtgtgccta ggaaagaaag
     9601 tgatccacgc ggtcggccct gatttccgga agcacccaga agcagaagcc ttgaaattgc
     9661 tacaaaacgc ctaccatgca gtggcagact tagtaaatga acataacatc aagtctgtcg
     9721 ccattccact gctatctaca ggcatttacg cagccggaaa agaccgcctt gaagtatcac
     9781 ttaactgctt gacaaccgcg ctagacagaa ctgacgcgga cgtaaccatc tattgcctgg
     9841 ataagaagtg gaaggaaaga atcgacgcgg cactccaact taaggagtct gtaacagagc
     9901 tgaaggatga agatatggag atcgacgatg agttagtatg gattcatcca gacagttgct
     9961 tgaagggaag aaagggattc agtactacaa aaggaaaatt gtattcgtac ttcgaaggca
    10021 ccaaattcca tcaagcagca aaagacatgg cggagataaa ggtcctgttc cctaatgacc
    10081 aggaaagtaa tgaacaactg tgtgcctaca tattgggtga gaccatggaa gcaatccgcg
    10141 aaaagtgccc ggtcgaccat aacccgtcgt ctagcccgcc caaaacgttg ccgtgccttt
    10201 gcatgtatgc catgacgcca gaaagggtcc acagacttag aagcaataac gtcaaagaag
    10261 ttacagtatg ctcctccacc ccccttccta agcacaaaat taagaatgtt cagaaggttc
    10321 agtgcacgaa agtagtcctg tttaatccgc acactcccgc attcgttccc gcccgtaagt
    10381 acatagaagt gccagaacag cctaccgctc ctcctgcaca ggccgaggag gcccccgaag
    10441 ttgtagcgac accgtcacca tctacagctg ataacacctc gcttgatgtc acagacatct
    10501 cactggatat ggatgacagt agcgaaggct cacttttttc gagctttagc ggatcggaca
    10561 actctattac tagtatggac agttggtcgt caggacctag ttcactagag atagtagacc
    10621 gaaggcaggt ggtggtggct gacgttcatg ccgtccaaga gcctgcccct attccaccgc
    10681 caaggctaaa gaagatggcc cgcctggcag cggcaagaaa agagcccact ccaccggcaa
    10741 gcaatagctc tgagtccctc cacctctctt ttggtggggt atccatgtcc ctcggatcaa
    10801 ttttcgacgg agagacggcc cgccaggcag cggtacaacc cctggcaaca ggccccacgg
    10861 atgtgcctat gtctttcgga tcgttttccg acggagagat tgatgagctg agccgcagag
    10921 taactgagtc cgaacccgtc ctgtttggat catttgaacc gggcgaagtg aactcaatta
    10981 tatcgtcccg atcagccgta tcttttccac tacgcaagca gagacgtaga cgcaggagca
    11041 ggaggactga atactgacta accggggtag gtgggtacat attttcgacg gacacaggcc
    11101 ctgggcactt gcaaaagaag tccgttctgc agaaccagct tacagaaccg accttggagc
    11161 gcaatgtcct ggaaagaatt catgccccgg tgctcgacac gtcgaaagag gaacaactca
    11221 aactcaggta ccagatgatg cccaccgaag ccaacaaaag taggtaccag tctcgtaaag
    11281 tagaaaatca gaaagccata accactgagc gactactgtc aggactacga ctgtataact
    11341 ctgccacaga tcagccagaa tgctataaga tcacctatcc gaaaccattg tactccagta
    11401 gcgtaccggc gaactactcc gatccacagt tcgctgtagc tgtctgtaac aactatctgc
    11461 atgagaacta tccgacagta gcatcttatc agattactga cgagtacgat gcttacttgg
    11521 atatggtaga cgggacagtc gcctgcctgg atactgcaac cttctgcccc gctaagctta
    11581 gaagttaccc gaaaaaacat gagtatagag ccccgaatat ccgcagtgcg gttccatcag
    11641 cgatgcagaa cacgctacaa aatgtgctca ttgccgcaac taaaagaaat tgcaacgtca
    11701 cgcagatgcg tgaactgcca acactggact cagcgacatt caatgtcgaa tgctttcgaa
    11761 aatatgcatg taatgacgag tattgggagg agttcgctcg gaagccaatt aggattacca
    11821 ctgagtttgt caccgcatat gtagctagac tgaaaggccc taaggccgcc gcactatttg
    11881 caaagacgta taatttggtc ccattgcaag aagtgcctat ggatagattc gtcatggaca
    11941 tgaaaagaga cgtgaaagtt acaccaggca cgaaacacac agaagaaaga ccgaaagtac
    12001 aagtgataca agccgcagaa cccctggcga ctgcttactt atgcgggatt caccgggaat
    12061 tagtgcgtag gcttacggcc gtcttgcttc caaacattca cacgcttttt gacatgtcgg
    12121 cggaggattt tgatgcaatc atagcagaac acttcaagca aggcgacccg gtactggaga
    12181 cggatatcgc atcattcgac aaaagccaag acgacgctat ggcgttaacc ggtctgatga
    12241 tcttggagga cctgggtgtg gatcaaccac tactcgactt gatcgagtgc gcctttggag
    12301 aaatatcatc cacccatcta cctacgggta ctcgttttaa attcggggcg atgatgaaat
    12361 ccggaatgtt cctcacactt tttgtcaaca cagttttgaa tgtcgttatc gccagcagag
    12421 tactagaaga gcggcttaaa acgtccagat gtgcagcgtt cattggcgac gacaacatca
    12481 tacatggagt agtatctgac aaagaaatgg ctgagaggtg cgccacctgg ctcaacatgg
    12541 aggttaagat catcgacgca gtcatcggtg agagaccacc ttacttctgc ggcggattta
    12601 tcttgcaaga ttcggttact tccacagcgt gccgcgtggc
//
