LOCUS       sequence-46150-e       14796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 14796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1295
FEATURES             Location/Qualifiers
     source          1..14796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             345..1052
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1059..1766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1773..2480
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             complement(2938..3741)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILIDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3742..3957)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      4188..5352
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     complement(5369..5388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5488..5510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5548..5566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5634..5738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5739..5807,5808..6599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5957..5976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6770..7358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7259..7278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatcccctg aaaaggctgt ttaagttggg taaaccgctc ccagccgacg acgagcaaga
       61 cgaagacaga agacgcgctc tgctagatga aacaaaggcg tggtttagag taggtataac
      121 aggcacttta gcagtggccg tgacgacccg gtatgaggta gacaatatta cacctgtcct
      181 actggcattg agaacttttg cccagagcaa aagagcattc caagccatca gaggggaaat
      241 aaagcatctc tacggtggtc ctaaatagtc agcatagtac atttcatctg actaatacta
      301 caacaccacc acctctagag aagcttcgta cgctgcaggt cgacatggtg agcaagggcg
      361 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      421 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      481 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      541 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      601 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      661 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      721 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      781 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      841 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      901 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      961 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     1021 actccaccgg cggcatggac gagctgtaca agctcgacat ggtgagcaag ggcgaggagg
     1081 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1141 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1201 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1261 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1321 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1381 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1441 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1501 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1561 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1621 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1681 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1741 ccggcggcat ggacgagctg tacaagctcg acatggtgag caagggcgag gaggataaca
     1801 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1861 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1921 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1981 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2041 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2101 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2161 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2221 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2281 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2341 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2401 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2461 gcatggacga gctgtacaag ctcgagtgag cgcgccactt cgccttgggc ccaatgatcc
     2521 gaccagcaaa actcgatgta cttccgagga actgatgtgc ataatgcatc aggctggtac
     2581 attagatccc cgcttaccgc gggcaatata gcaacactaa aaactcgatg tacttccgag
     2641 gaagcgcagt gcataatgct gcgcagtgtt gccacataac cactatatta accatttatc
     2701 tagcggacgc caaaaactca atgtatttct gaggaagcgt ggtgcataat gccacgcagc
     2761 gtctgcataa cttttattat ttcttttatt aatcaacaaa attttgtttt taacatttca
     2821 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aggggaattc ctcgattaat taagcggccg
     2881 ctcgagagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     2941 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     3001 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     3061 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     3121 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     3181 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     3241 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     3301 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     3361 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     3421 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     3481 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     3541 atcaatcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     3601 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     3661 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     3721 cttatatgta gctttcgaca tgatttatct tcgtttcggt ttttgttctg tgcagttggg
     3781 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     3841 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     3901 ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagcactt
     3961 gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta
     4021 tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt
     4081 cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg
     4141 ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg
     4201 tgcttcattt tgtagaacaa aaaagcaacg cgagagcgct aatttttcaa acaaagaatc
     4261 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     4321 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     4381 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     4441 aagaatctat acttcctttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     4501 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     4561 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctatcttctc
     4621 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     4681 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     4741 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     4801 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     4861 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     4921 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     4981 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     5041 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     5101 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     5161 tactttctag ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
     5221 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
     5281 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
     5341 gcttaaatgc gttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5401 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5521 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5581 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5641 cccctatttg tttatttttc taaatacctt caaatatgta tccgctcatg agacaataac
     5701 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5761 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5821 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5881 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5941 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     6001 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6061 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6121 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6181 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6241 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6301 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6361 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6421 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6481 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6541 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6601 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6661 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6721 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6901 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7141 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcgt
     7381 atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     7441 atacgattta ggggacacta tagattgacg gcgtagtaca cactattgaa tcaaacagcc
     7501 gaccaattgc actaccatca caatggagaa gccagtagta aacgtagacg tagaccccca
     7561 gagtccgttt gtcgtgcaac tgcaaaaaag cttcccgcaa tttgaggtag tagcacagca
     7621 ggtcactcca aatgaccatg ctaatgccag agcattttcg catctggcca gtaaactaat
     7681 cgagctggag gttcctacca cagcgacgat cttggacata ggcagcgcac cggctcgtag
     7741 aatgttttcc gagcaccagt atcattgtgt ctgccccatg cgtagtccag aagacccgga
     7801 ccgcatgatg aaatacgcca gtaaactggc ggaaaaagcg tgcaagatta caaacaagaa
     7861 cttgcatgag aagattaagg atctccggac cgtacttgat acgccggatg ctgaaacacc
     7921 atcgctctgc tttcacaacg atgttacctg caacatgcgt gccgaatatt ccgtcatgca
     7981 ggacgtgtat atcaacgctc ccggaactat ctatcatcag gctatgaaag gcgtgcggac
     8041 cctgtactgg attggcttcg acaccaccca gttcatgttc tcggctatgg caggttcgta
     8101 ccctgcgtac aacaccaact gggccgacga gaaagtcctt gaagcgcgta acatcggact
     8161 ttgcagcaca aagctgagtg aaggtaggac aggaaaattg tcgataatga ggaagaagga
     8221 gttgaagccc gggtcgcggg tttatttctc cgtaggatcg acactttatc cagaacacag
     8281 agccagcttg cagagctggc atcttccatc ggtgttccac ttgaatggaa agcagtcgta
     8341 cacttgccgc tgtgatacag tggtgagttg cgaaggctac gtagtgaaga aaatcaccat
     8401 cagtcccggg atcacgggag aaaccgtggg atacgcggtt acacacaata gcgagggctt
     8461 cttgctatgc aaagttactg acacagtaaa aggagaacgg gtatcgttcc ctgtgtgcac
     8521 gtacatcccg gccaccatat gcgatcagat gactggtata atggccacgg atatatcacc
     8581 tgacgatgca caaaaacttc tggttgggct caaccagcga attgtcatta acggtaggac
     8641 taacaggaac accaacacca tgcaaaatta ccttctgccg atcatagcac aagggttcag
     8701 caaatgggct aaggagcgca aggatgatct tgataacgag aaaatgctgg gtactagaga
     8761 acgcaagctt acgtatggct gcttgtgggc gtttcgcact aagaaagtac attcgtttta
     8821 tcgcccacct ggaacgcaga cctgcgtaaa agtcccagcc tcttttagcg cttttcccat
     8881 gtcgtccgta tggacgacct ctttgcccat gtcgctgagg cagaaattga aactggcatt
     8941 gcaaccaaag aaggaggaaa aactgctgca ggtctcggag gaattagtca tggaggccaa
     9001 ggctgctttt gaggatgctc aggaggaagc cagagcggag aagctccgag aagcacttcc
     9061 accattagtg gcagacaaag gcatcgaggc agccgcagaa gttgtctgcg aagtggaggg
     9121 gctccaggcg gacatcggag cagcattagt tgaaaccccg cgcggtcacg taaggataat
     9181 acctcaagca aatgaccgta tgatcggaca gtatatcgtt gtctcgccaa actctgtgct
     9241 gaagaatgcc aaactcgcac cagcgcaccc gctagcagat caggttaaga tcataacaca
     9301 ctccggaaga tcaggaaggt acgcggtcga accatacgac gctaaagtac tgatgccagc
     9361 aggaggtgcc gtaccatggc cagaattcct agcactgagt gagagcgcca cgttagtgta
     9421 caacgaaaga gagtttgtga accgcaaact ataccacatt gccatgcatg gccccgccaa
     9481 gaatacagaa gaggagcagt acaaggttac aaaggcagag cttgcagaaa cagagtacgt
     9541 gtttgacgtg gacaagaagc gttgcgttaa gaaggaagaa gcctcaggtc tggtcctctc
     9601 gggagaactg accaaccctc cctatcatga gctagctctg gagggactga agacccgacc
     9661 tgcggtcccg tacaaggtcg aaacaatagg agtgataggc acaccggggt cgggcaagtc
     9721 agctattatc aagtcaactg tcacggcacg agatcttgtt accagcggaa agaaagaaaa
     9781 ttgtcgcgaa attgaggccg acgtgctaag actgaggggt atgcagatta cgtcgaagac
     9841 agtagattcg gttatgctca acggatgcca caaagccgta gaagtgctgt acgttgacga
     9901 agcgttcgcg tgccacgcag gagcactact tgccttgatt gctatcgtca ggccccgcaa
     9961 gaaggtagta ctatgcggag accccatgca atgcggattc ttcaacatga tgcaactaaa
    10021 ggtacatttc aatcaccctg aaaaagacat atgcaccaag acattctaca agtatatctc
    10081 ccggcgttgc acacagccag ttacagctat tgtatcgaca ctgcattacg atggaaagat
    10141 gaaaaccacg aacccgtgca agaagaacat tgaaatcgat attacagggg ccacaaagcc
    10201 gaagccaggg gatatcatcc tgacatgttt ccgcgggtgg gttaagcaat tgcaaatcga
    10261 ctatcccgga catgaagtaa tgacagccgc ggcctcacaa gggctaacca gaaaaggagt
    10321 gtatgccgtc cggcaaaaag tcaatgaaaa cccactgtac gcgatcacat cagagcatgt
    10381 gaacgtgttg ctcacccgca ctgaggacag gctagtgtgg aaaaccttgc agggcgaccc
    10441 atggattaag cagcccacta acatacctaa aggaaacttt caggctacta tagaggactg
    10501 ggaagctgaa cacaagggaa taattgctgc aataaacagc cccactcccc gtgccaatcc
    10561 gttcagctgc aagaccaacg tttgctgggc gaaagcattg gaaccgatac tagccacggc
    10621 cggtatcgta cttaccggtt gccagtggag cgaactgttc ccacagtttg cggatgacaa
    10681 accacattcg gccatttacg ccttagacgt aatttgcatt aagtttttcg gcatggactt
    10741 gacaagcgga ctgttttcta aacagagcat cccactaacg taccatcccg ccgattcagc
    10801 gaggccggta gctcattggg acaacagccc aggaacccgc aagtatgggt acgatcacgc
    10861 cattgccgcc gaactctccc gtagatttcc ggtgttccag ctagctggga agggcacaca
    10921 acttgatttg cagacgggga gaaccagagt tatctctgca cagcataacc tggtcccggt
    10981 gaaccgcaat cttcctcacg ccttagtccc cgagtacaag gagaagcaac ccggcccggt
    11041 caaaaaattc ttgaaccagt tcaaacacca ctcagtactt gtggtatcag aggaaaaaat
    11101 tgaagctccc cgtaagagaa tcgaatggat cgccccgatt ggcatagccg gtgcagataa
    11161 gaactacaac ctggctttcg ggtttccgcc gcaggcacgg tacgacctgg tgttcatcaa
    11221 cattggaact aaatacagaa accaccactt tcagcagtgc gaagaccatg cggcgacctt
    11281 aaaaaccctt tcgcgttcgg ccctgaattg ccttaaccca ggaggcaccc tcgtggtgaa
    11341 gtcctatggc tacgccgacc gcaacagtga ggacgtagtc accgctcttg ccagaaagtt
    11401 tgtcagggtg tctgcagcga gaccagattg tgtctcaagc aatacagaaa tgtacctgat
    11461 tttccgacaa ctagacaaca gccgtacacg gcaattcacc ccgcaccatc tgaattgcgt
    11521 gatttcgtcc gtgtatgagg gtacaagaga tggagttgga gccgcgccgt cataccgcac
    11581 caaaagggag aatattgctg actgtcaaga ggaagcagtt gtcaacgcag ccaatccgct
    11641 gggtagacca ggcgaaggag tctgccgtgc catctataaa cgttggccga ccagttttac
    11701 cgattcagcc acggagacag gcaccgcaag aatgactgtg tgcctaggaa agaaagtgat
    11761 ccacgcggtc ggccctgatt tccggaagca cccagaagca gaagccttga aattgctaca
    11821 aaacgcctac catgcagtgg cagacttagt aaatgaacat aacatcaagt ctgtcgccat
    11881 tccactgcta tctacaggca tttacgcagc cggaaaagac cgccttgaag tatcacttaa
    11941 ctgcttgaca accgcgctag acagaactga cgcggacgta accatctatt gcctggataa
    12001 gaagtggaag gaaagaatcg acgcggcact ccaacttaag gagtctgtaa cagagctgaa
    12061 ggatgaagat atggagatcg acgatgagtt agtatggatt catccagaca gttgcttgaa
    12121 gggaagaaag ggattcagta ctacaaaagg aaaattgtat tcgtacttcg aaggcaccaa
    12181 attccatcaa gcagcaaaag acatggcgga gataaaggtc ctgttcccta atgaccagga
    12241 aagtaatgaa caactgtgtg cctacatatt gggtgagacc atggaagcaa tccgcgaaaa
    12301 gtgcccggtc gaccataacc cgtcgtctag cccgcccaaa acgttgccgt gcctttgcat
    12361 gtatgccatg acgccagaaa gggtccacag acttagaagc aataacgtca aagaagttac
    12421 agtatgctcc tccacccccc ttcctaagca caaaattaag aatgttcaga aggttcagtg
    12481 cacgaaagta gtcctgttta atccgcacac tcccgcattc gttcccgccc gtaagtacat
    12541 agaagtgcca gaacagccta ccgctcctcc tgcacaggcc gaggaggccc ccgaagttgt
    12601 agcgacaccg tcaccatcta cagctgataa cacctcgctt gatgtcacag acatctcact
    12661 ggatatggat gacagtagcg aaggctcact tttttcgagc tttagcggat cggacaactc
    12721 tattactagt atggacagtt ggtcgtcagg acctagttca ctagagatag tagaccgaag
    12781 gcaggtggtg gtggctgacg ttcatgccgt ccaagagcct gcccctattc caccgccaag
    12841 gctaaagaag atggcccgcc tggcagcggc aagaaaagag cccactccac cggcaagcaa
    12901 tagctctgag tccctccacc tctcttttgg tggggtatcc atgtccctcg gatcaatttt
    12961 cgacggagag acggcccgcc aggcagcggt acaacccctg gcaacaggcc ccacggatgt
    13021 gcctatgtct ttcggatcgt tttccgacgg agagattgat gagctgagcc gcagagtaac
    13081 tgagtccgaa cccgtcctgt ttggatcatt tgaaccgggc gaagtgaact caattatatc
    13141 gtcccgatca gccgtatctt ttccactacg caagcagaga cgtagacgca ggagcaggag
    13201 gactgaatac tgactaaccg gggtaggtgg gtacatattt tcgacggaca caggccctgg
    13261 gcacttgcaa aagaagtccg ttctgcagaa ccagcttaca gaaccgacct tggagcgcaa
    13321 tgtcctggaa agaattcatg ccccggtgct cgacacgtcg aaagaggaac aactcaaact
    13381 caggtaccag atgatgccca ccgaagccaa caaaagtagg taccagtctc gtaaagtaga
    13441 aaatcagaaa gccataacca ctgagcgact actgtcagga ctacgactgt ataactctgc
    13501 cacagatcag ccagaatgct ataagatcac ctatccgaaa ccattgtact ccagtagcgt
    13561 accggcgaac tactccgatc cacagttcgc tgtagctgtc tgtaacaact atctgcatga
    13621 gaactatccg acagtagcat cttatcagat tactgacgag tacgatgctt acttggatat
    13681 ggtagacggg acagtcgcct gcctggatac tgcaaccttc tgccccgcta agcttagaag
    13741 ttacccgaaa aaacatgagt atagagcccc gaatatccgc agtgcggttc catcagcgat
    13801 gcagaacacg ctacaaaatg tgctcattgc cgcaactaaa agaaattgca acgtcacgca
    13861 gatgcgtgaa ctgccaacac tggactcagc gacattcaat gtcgaatgct ttcgaaaata
    13921 tgcatgtaat gacgagtatt gggaggagtt cgctcggaag ccaattagga ttaccactga
    13981 gtttgtcacc gcatatgtag ctagactgaa aggccctaag gccgccgcac tatttgcaaa
    14041 gacgtataat ttggtcccat tgcaagaagt gcctatggat agattcgtca tggacatgaa
    14101 aagagacgtg aaagttacac caggcacgaa acacacagaa gaaagaccga aagtacaagt
    14161 gatacaagcc gcagaacccc tggcgactgc ttacttatgc gggattcacc gggaattagt
    14221 gcgtaggctt acggccgtct tgcttccaaa cattcacacg ctttttgaca tgtcggcgga
    14281 ggattttgat gcaatcatag cagaacactt caagcaaggc gacccggtac tggagacgga
    14341 tatcgcatca ttcgacaaaa gccaagacga cgctatggcg ttaaccggtc tgatgatctt
    14401 ggaggacctg ggtgtggatc aaccactact cgacttgatc gagtgcgcct ttggagaaat
    14461 atcatccacc catctaccta cgggtactcg ttttaaattc ggggcgatga tgaaatccgg
    14521 aatgttcctc acactttttg tcaacacagt tttgaatgtc gttatcgcca gcagagtact
    14581 agaagagcgg cttaaaacgt ccagatgtgc agcgttcatt ggcgacgaca acatcataca
    14641 tggagtagta tctgacaaag aaatggctga gaggtgcgcc acctggctca acatggaggt
    14701 taagatcatc gacgcagtca tcggtgagag accaccttac ttctgcggcg gatttatctt
    14761 gcaagattcg gttacttcca cagcgtgccg cgtggc
//
