LOCUS       sequence-46151-e        5232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1320
FEATURES             Location/Qualifiers
     source          1..5232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     184..203
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             335..1366
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(1701..1719)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      1731..1920
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1936..1952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1936..1952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1949..1971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1960..1976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1984..1990,1991..2008,2009..2014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2029..2050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2285..2302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2419..2963)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2499..2518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3024..3683)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHP*FRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3598..3617
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3684..3786)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4173..4195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4188..4204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4304..5182)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(5209..5232,1..261)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc
       61 cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca
      121 aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt
      181 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc
      241 aactctctac tgtttctcca tacccgtttt tttgggctag caggaggaat tcaccatggt
      301 acccggggat cctctagagt cgacctgcag atctatgtcc aatttactga ccgtacacca
      361 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
      421 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
      481 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
      541 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
      601 gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga
      661 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
      721 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
      781 ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa
      841 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
      901 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
      961 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
     1021 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
     1081 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
     1141 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     1201 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     1261 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     1321 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattagccat taacgcgtaa
     1381 atgattgcta taattatttg atatttatgg tgacatatga gaaaggattt caacatcgac
     1441 ggaaaatatg tagtgctgtc tgtaagcact aatattcagt cgccagccgt cattgtcact
     1501 gtaaagctga gcgatagaat gcctgatatt gactcaatat ccgttgcgtt tcctgtcaaa
     1561 agtatgcgta gtgctgaaca tttcgtgatg aatgccaccg aggaagaagc acggcgcggt
     1621 tttgcntaaa gtgatgtctg agtttggcga actcttgggt aaggttggaa ttgtcgacct
     1681 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     1741 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     1801 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     1861 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     1921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctagcg
     2161 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     2221 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     2281 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     2341 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     2401 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     2461 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct
     2521 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     2581 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     2641 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     2701 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     2761 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     2821 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     2881 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     2941 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagg
     3001 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     3061 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     3121 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     3181 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     3241 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     3301 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     3361 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     3421 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaatta cggatgagca
     3481 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     3541 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     3601 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     3661 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     3721 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     3781 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     3841 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
     3901 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
     3961 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
     4021 cggcaaaagc accgccggac atcagcgcca ttcgccattc aggctgcgca actgttggga
     4081 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     4141 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4201 cagtctcgag acctgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     4261 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     4321 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     4381 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     4441 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     4501 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     4561 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     4621 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     4681 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     4741 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     4801 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     4861 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     4921 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     4981 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     5041 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     5101 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     5161 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     5221 gtccatattg ca
//
