LOCUS       sequence-46156-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for 
            ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(E123A)-mCherry
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg gacctggatg
       61 ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag ccccaagctc
      121 gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt ggcccctagt
      181 tctgggggca gctctagagc ggtaccggat ccccgggtac cggtgccacc atggattatg
      241 gaggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct gtggtggtga
      301 acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt gaatctcgcg
      361 gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca ggattcagca
      421 ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc tgggaggaga
      481 tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt gagtttaaga
      541 atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc tatgcagcct
      601 ggctgctcac ttgtcctgtc atccttatcc acctgagcaa cctcaccggc ctgagcaacg
      661 actacagcag gagaaccatg ggactccttg tctcagacat cgggactatc gtgtgggggg
      721 ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt ggattgtgct
      781 atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat catactgtgc
      841 caaagggtcg gtgccgccag gtcgtgaccg gcatgg
//
